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P46970 (NMD5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nonsense-mediated mRNA decay protein 5
Alternative name(s):
Karyopherin-119
Gene names
Name:NMD5
Synonyms:KAP119
Ordered Locus Names:YJR132W
ORF Names:J2112
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1048 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Active in protein import into the nucleus. Its major import substrate is transcription elongation factor TFIIS. Ref.4

Subunit structure

GTP-bound Ran dissociates the isolated NMD5/TFIIS complex. Ref.4

Subcellular location

Nucleus. Cytoplasm Ref.4 Ref.5.

Miscellaneous

Present with 13500 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 importin N-terminal domain.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCytoplasm
Nucleus
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein import into nucleus

Inferred from mutant phenotype PubMed 9755161. Source: SGD

   Cellular_componentcytoplasm

Inferred from direct assay Ref.4. Source: SGD

nucleus

Inferred from direct assay Ref.4. Source: SGD

   Molecular_functionprotein transporter activity

Inferred from direct assay Ref.4. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10481048Nonsense-mediated mRNA decay protein 5
PRO_0000096880

Regions

Domain24 – 10481Importin N-terminal

Amino acid modifications

Modified residue9771Phosphoserine Ref.7 Ref.8

Experimental info

Sequence conflict2581A → S in CAA89663. Ref.2
Sequence conflict5171V → I in AAA74490. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P46970 [UniParc].

Last modified September 21, 2011. Version 2.
Checksum: 90312C1AAE515949

FASTA1,048119,967
        10         20         30         40         50         60 
MDITELLQCF ACTLDHNAAV RTNAETHLKN ASKVPGFLGA CLDIIAADEV PENIKLSASL 

        70         80         90        100        110        120 
YFKNKITYGW SAGARQGSNE LLDSHVDPDE KPVVKDMLIK TMVSVSKTSP RCIRVLKSAL 

       130        140        150        160        170        180 
TVIISEDYPS KKWGNLLPNS LELLANEDIT VTYVGLLCLA EIFRTYRWKN NDERQDLEEL 

       190        200        210        220        230        240 
ILNYFPALLN YGANVLFQDG KYMNNEQIGE LVKLIIKIYK FVSYHDLPFT LQRSESFTPW 

       250        260        270        280        290        300 
ACFFVSIIQQ PLPQEVLAIS DIEVRSKNPW VKCKKWALAN LYRLFQRYAS TSLTRKFQYD 

       310        320        330        340        350        360 
EFKQMYCEEF LTQFLQVVFD QIEKWGTGQL WLSDECLYYI LNFVEQCVVQ KTTWKLVGPH 

       370        380        390        400        410        420 
YNVILQHVIF PLLKPTAETL EAFDNDPQEY INRNMDFWDV GYSPDLAALA LLTTCVTKRG 

       430        440        450        460        470        480 
KTTLQPTLEF MVSTLQSAVG DYNNIMLDNA LQIESCLRIF SSIIDRLITK DSPFASEMEK 

       490        500        510        520        530        540 
FILTYVLPFF KSQYGFLQSR VCDICSKLGS MDFKDPVITS TIYEGVMNCL NNSSNSLPVE 

       550        560        570        580        590        600 
LTAALALQTF ISDDQFNMKL SEHVVPTMQK LLSLSNDFES DVISGVMQDF VEQFAEQLQP 

       610        620        630        640        650        660 
FGVELMNTLV QQFLKLAIDL HETSNLDPDS FTNVDSIPDE SDKQMAALGI LSTTISILLS 

       670        680        690        700        710        720 
FENSPEILKN LEQSFYPAAE FILKNDIEDF YRECCEFVEN STFLLRDITP ISWKILELIG 

       730        740        750        760        770        780 
ECNRKPDSMV SYYLSDFMLA LNNILIYGRN ELKKNEFYTK IIFEIYQKAV TAEDNSLDDL 

       790        800        810        820        830        840 
RVVFDLSQEL VLALDDSLPQ QYRERLLADV VGSILTQKNE LKTNVVFSVT AFNVVISNMI 

       850        860        870        880        890        900 
TEPLITLQYL KQQGCLEIFF QTWITDYIPN YKRCYDIKLS VLALLKIILK LESNDYSVLN 

       910        920        930        940        950        960 
LENLVPQLGS IVTQLASRLP TALRQLANQR KEFSSSGFEE DTKWDENFLD VGDDDENDDE 

       970        980        990       1000       1010       1020 
GDLTEKYLEL IKNRADSLDF VDGYDAKETF DDLEEDPLTG SILDTVDVYK VFKESIANLQ 

      1030       1040 
HVDSNRYQGI LRHLTPADQE LFMGIMNA 

« Hide

References

« Hide 'large scale' references
[1]He F., Jacobson A.
Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 258.
Strain: ATCC 204508 / S288c.
[4]"A novel nuclear import pathway for the transcription factor TFIIS."
Albertini M., Pemberton L.F., Rosenblum J.S., Blobel G.
J. Cell Biol. 143:1447-1455(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-977, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-977, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U31375 Genomic DNA. Translation: AAA74490.1.
Z49632 Genomic DNA. Translation: CAA89663.1.
BK006943 Genomic DNA. Translation: DAA08916.2.
PIRS57155.
RefSeqNP_012666.2. NM_001181790.2.

3D structure databases

ProteinModelPortalP46970.
SMRP46970. Positions 136-162.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33887. 73 interactions.
DIPDIP-2326N.
IntActP46970. 36 interactions.
MINTMINT-561722.
STRING4932.YJR132W.

Proteomic databases

PaxDbP46970.
PeptideAtlasP46970.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR132W; YJR132W; YJR132W.
GeneID853596.
KEGGsce:YJR132W.

Organism-specific databases

CYGDYJR132w.
SGDS000003893. NMD5.

Phylogenomic databases

eggNOGCOG5656.
GeneTreeENSGT00550000074736.
HOGENOMHOG000248078.
OMAPECWELL.
OrthoDBEOG7QK0MD.

Enzyme and pathway databases

BioCycYEAST:G3O-31750-MONOMER.

Gene expression databases

GenevestigatorP46970.

Family and domain databases

Gene3D1.25.10.10. 2 hits.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR001494. Importin-beta_N.
[Graphical view]
PfamPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
PROSITEPS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974412.

Entry information

Entry nameNMD5_YEAST
AccessionPrimary (citable) accession number: P46970
Secondary accession number(s): D6VWV0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: September 21, 2011
Last modified: March 19, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families