P46970 (NMD5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nonsense-mediated mRNA decay protein 5

Alternative name(s):
Karyopherin-119

Gene names

Name:	NMD5
Synonyms:	KAP119
Ordered Locus Names:	YJR132W
ORF Names:	J2112

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1048 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Active in protein import into the nucleus. Its major import substrate is transcription elongation factor TFIIS. Ref.4
Subunit structure	GTP-bound Ran dissociates the isolated NMD5/TFIIS complex. Ref.4
Subcellular location	Nucleus. Cytoplasm Ref.4 Ref.5.
Miscellaneous	Present with 13500 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Contains 1 importin N-terminal domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein import into nucleus Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay Ref.4. Source: SGD nucleus Inferred from direct assay Ref.4. Source: SGD
Molecular function	binding Inferred from electronic annotation. Source: InterPro protein transporter activity Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1048	1048	Nonsense-mediated mRNA decay protein 5		PRO_0000096880
Regions
	Domain	24 – 104	81	Importin N-terminal
Amino acid modifications
	Modified residue	977	1	Phosphoserine Ref.7 Ref.8
Experimental info
	Sequence conflict	258	1	A → S in CAA89663. Ref.2
	Sequence conflict	517	1	V → I in AAA74490. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P46970 [UniParc]. Last modified September 21, 2011. Version 2. Checksum: 90312C1AAE515949	FASTA	1,048	119,967
	10 20 30 40 50 60 MDITELLQCF ACTLDHNAAV RTNAETHLKN ASKVPGFLGA CLDIIAADEV PENIKLSASL 70 80 90 100 110 120 YFKNKITYGW SAGARQGSNE LLDSHVDPDE KPVVKDMLIK TMVSVSKTSP RCIRVLKSAL 130 140 150 160 170 180 TVIISEDYPS KKWGNLLPNS LELLANEDIT VTYVGLLCLA EIFRTYRWKN NDERQDLEEL 190 200 210 220 230 240 ILNYFPALLN YGANVLFQDG KYMNNEQIGE LVKLIIKIYK FVSYHDLPFT LQRSESFTPW 250 260 270 280 290 300 ACFFVSIIQQ PLPQEVLAIS DIEVRSKNPW VKCKKWALAN LYRLFQRYAS TSLTRKFQYD 310 320 330 340 350 360 EFKQMYCEEF LTQFLQVVFD QIEKWGTGQL WLSDECLYYI LNFVEQCVVQ KTTWKLVGPH 370 380 390 400 410 420 YNVILQHVIF PLLKPTAETL EAFDNDPQEY INRNMDFWDV GYSPDLAALA LLTTCVTKRG 430 440 450 460 470 480 KTTLQPTLEF MVSTLQSAVG DYNNIMLDNA LQIESCLRIF SSIIDRLITK DSPFASEMEK 490 500 510 520 530 540 FILTYVLPFF KSQYGFLQSR VCDICSKLGS MDFKDPVITS TIYEGVMNCL NNSSNSLPVE 550 560 570 580 590 600 LTAALALQTF ISDDQFNMKL SEHVVPTMQK LLSLSNDFES DVISGVMQDF VEQFAEQLQP 610 620 630 640 650 660 FGVELMNTLV QQFLKLAIDL HETSNLDPDS FTNVDSIPDE SDKQMAALGI LSTTISILLS 670 680 690 700 710 720 FENSPEILKN LEQSFYPAAE FILKNDIEDF YRECCEFVEN STFLLRDITP ISWKILELIG 730 740 750 760 770 780 ECNRKPDSMV SYYLSDFMLA LNNILIYGRN ELKKNEFYTK IIFEIYQKAV TAEDNSLDDL 790 800 810 820 830 840 RVVFDLSQEL VLALDDSLPQ QYRERLLADV VGSILTQKNE LKTNVVFSVT AFNVVISNMI 850 860 870 880 890 900 TEPLITLQYL KQQGCLEIFF QTWITDYIPN YKRCYDIKLS VLALLKIILK LESNDYSVLN 910 920 930 940 950 960 LENLVPQLGS IVTQLASRLP TALRQLANQR KEFSSSGFEE DTKWDENFLD VGDDDENDDE 970 980 990 1000 1010 1020 GDLTEKYLEL IKNRADSLDF VDGYDAKETF DDLEEDPLTG SILDTVDVYK VFKESIANLQ 1030 1040 HVDSNRYQGI LRHLTPADQE LFMGIMNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	He F., Jacobson A. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 258. Strain: ATCC 204508 / S288c.
[4]	"A novel nuclear import pathway for the transcription factor TFIIS." Albertini M., Pemberton L.F., Rosenblum J.S., Blobel G. J. Cell Biol. 143:1447-1455(1998) [PubMed: 9852143] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-977, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-977, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U31375 Genomic DNA. Translation: AAA74490.1. Z49632 Genomic DNA. Translation: CAA89663.1. BK006943 Genomic DNA. Translation: DAA08916.2.
PIR	S57155.
RefSeq	NP_012666.2. NM_001181790.2.
3D structure databases
ProteinModelPortal	P46970.
SMR	P46970. Positions 136-164.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2326N.
IntAct	P46970. 40 interactions.
MINT	MINT-561722.
STRING	P46970.
Proteomic databases
PeptideAtlas	P46970.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	853596.
KEGG	sce:YJR132W.
NMPDR	fig|4932.3.peg.3641.
Organism-specific databases
CYGD	YJR132w.
SGD	S000003893. NMD5.
Phylogenomic databases
eggNOG	fuNOG05523.
GeneTree	EFGT00050000003792.
HOGENOM	HBG397193.
OMA	TSSKEMS.
OrthoDB	EOG4V9XZR.
Gene expression databases
ArrayExpress	P46970.
Genevestigator	P46970.
GermOnline	YJR132W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR001494. Importin-beta_N. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 2 hits.
Pfam	PF03810. IBN_N. 1 hit. [Graphical view]
SMART	SM00913. IBN_N. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50166. IMPORTIN_B_NT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974412.

Entry information

Entry name

NMD5_YEAST

Accession

Primary (citable) accession number: P46970
Secondary accession number(s): D6VWV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	September 21, 2011
Last modified:	January 25, 2012
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program