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Protein

Ribulose-phosphate 3-epimerase

Gene

RPE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate (By similarity). Involved in the protective response to oxidative stress.By similarity1 Publication

Catalytic activityi

D-ribulose 5-phosphate = D-xylulose 5-phosphate.

Cofactori

Co2+By similarity, Fe2+By similarity, Mn2+By similarity, Zn2+By similarityNote: Binds 1 divalent metal cation per subunit. Active with Co2+, Fe2+, Mn2+ and Zn2+.By similarity

Pathwayi: pentose phosphate pathway

This protein is involved in step 1 of the subpathway that synthesizes D-xylulose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage).
Proteins known to be involved in this subpathway in this organism are:
  1. Ribulose-phosphate 3-epimerase (RPE1)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-xylulose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9SubstrateBy similarity1
Metal bindingi34Divalent metal cationBy similarity1
Active sitei36Proton acceptorBy similarity1
Metal bindingi36Divalent metal cationBy similarity1
Metal bindingi80Divalent metal cationBy similarity1
Binding sitei80SubstrateBy similarity1
Active sitei185Proton donorBy similarity1
Metal bindingi185Divalent metal cationBy similarity1
Binding sitei187Substrate; via amide nitrogenBy similarity1

GO - Molecular functioni

  • metal ion binding Source: GO_Central
  • ribulose-phosphate 3-epimerase activity Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Cobalt, Iron, Manganese, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YJL121C-MONOMER.
ReactomeiR-SCE-71336. Pentose phosphate pathway (hexose monophosphate shunt).
UniPathwayiUPA00115; UER00411.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose-phosphate 3-epimerase (EC:5.1.3.1)
Alternative name(s):
Pentose-5-phosphate 3-epimerase
Short name:
PPE
RPE
Gene namesi
Name:RPE1
Synonyms:EPI1, POS18
Ordered Locus Names:YJL121C
ORF Names:J0731
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL121C.
SGDiS000003657. RPE1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001715951 – 238Ribulose-phosphate 3-epimeraseAdd BLAST238

Proteomic databases

MaxQBiP46969.
PRIDEiP46969.

Interactioni

Protein-protein interaction databases

BioGridi33635. 62 interactors.
MINTiMINT-4490966.

Structurei

3D structure databases

ProteinModelPortaliP46969.
SMRiP46969.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni156 – 159Substrate bindingBy similarity4
Regioni207 – 208Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000001447.
HOGENOMiHOG000259349.
InParanoidiP46969.
KOiK01783.
OMAiKTIDVCA.
OrthoDBiEOG092C4JF2.

Family and domain databases

CDDicd00429. RPE. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR026019. Ribul_P_3_epim.
IPR000056. Ribul_P_3_epim-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PANTHERiPTHR11749. PTHR11749. 1 hit.
PfamiPF00834. Ribul_P_3_epim. 1 hit.
[Graphical view]
PIRSFiPIRSF001461. RPE. 1 hit.
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01163. rpe. 1 hit.
PROSITEiPS01085. RIBUL_P_3_EPIMER_1. 1 hit.
PS01086. RIBUL_P_3_EPIMER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46969-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKPIIAPSI LASDFANLGC ECHKVINAGA DWLHIDVMDG HFVPNITLGQ
60 70 80 90 100
PIVTSLRRSV PRPGDASNTE KKPTAFFDCH MMVENPEKWV DDFAKCGADQ
110 120 130 140 150
FTFHYEATQD PLHLVKLIKS KGIKAACAIK PGTSVDVLFE LAPHLDMALV
160 170 180 190 200
MTVEPGFGGQ KFMEDMMPKV ETLRAKFPHL NIQVDGGLGK ETIPKAAKAG
210 220 230
ANVIVAGTSV FTAADPHDVI SFMKEEVSKE LRSRDLLD
Length:238
Mass (Da):25,967
Last modified:November 1, 1995 - v1
Checksum:i7EEFA5EBE1D3DF8F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83571 Genomic DNA. Translation: CAA58554.1.
Z49396 Genomic DNA. Translation: CAA89415.1.
AY557858 Genomic DNA. Translation: AAS56184.1.
BK006943 Genomic DNA. Translation: DAA08681.1.
PIRiS72623.
RefSeqiNP_012414.1. NM_001181554.1.

Genome annotation databases

EnsemblFungiiYJL121C; YJL121C; YJL121C.
GeneIDi853322.
KEGGisce:YJL121C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83571 Genomic DNA. Translation: CAA58554.1.
Z49396 Genomic DNA. Translation: CAA89415.1.
AY557858 Genomic DNA. Translation: AAS56184.1.
BK006943 Genomic DNA. Translation: DAA08681.1.
PIRiS72623.
RefSeqiNP_012414.1. NM_001181554.1.

3D structure databases

ProteinModelPortaliP46969.
SMRiP46969.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33635. 62 interactors.
MINTiMINT-4490966.

Proteomic databases

MaxQBiP46969.
PRIDEiP46969.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL121C; YJL121C; YJL121C.
GeneIDi853322.
KEGGisce:YJL121C.

Organism-specific databases

EuPathDBiFungiDB:YJL121C.
SGDiS000003657. RPE1.

Phylogenomic databases

GeneTreeiENSGT00390000001447.
HOGENOMiHOG000259349.
InParanoidiP46969.
KOiK01783.
OMAiKTIDVCA.
OrthoDBiEOG092C4JF2.

Enzyme and pathway databases

UniPathwayiUPA00115; UER00411.
BioCyciYEAST:YJL121C-MONOMER.
ReactomeiR-SCE-71336. Pentose phosphate pathway (hexose monophosphate shunt).

Miscellaneous databases

PROiP46969.

Family and domain databases

CDDicd00429. RPE. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR026019. Ribul_P_3_epim.
IPR000056. Ribul_P_3_epim-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PANTHERiPTHR11749. PTHR11749. 1 hit.
PfamiPF00834. Ribul_P_3_epim. 1 hit.
[Graphical view]
PIRSFiPIRSF001461. RPE. 1 hit.
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01163. rpe. 1 hit.
PROSITEiPS01085. RIBUL_P_3_EPIMER_1. 1 hit.
PS01086. RIBUL_P_3_EPIMER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPE_YEAST
AccessioniPrimary (citable) accession number: P46969
Secondary accession number(s): D6VW65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3310 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.