P46967 (DAD1_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit dad1
Short name=Oligosaccharyl transferase subunit dad1
EC=2.4.1.119

Alternative name(s):
Defender against cell death 1
Short name=DAD-1

Gene names

Name:

dad1

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	113 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity. Loss of the DAD1 protein triggers apoptosis.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the DAD/OST2 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Gene Ontology (GO)
Biological process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW protein glycosylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW oligosaccharyltransferase complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 113	113	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit dad1		PRO_0000124016
Regions
	Transmembrane	31 – 51	21	Helical; Potential
	Transmembrane	53 – 73	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P46967 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: B8B4FB18524D59B2	FASTA	113	12,596
	10 20 30 40 50 60 MSVSVFSVVS RFLDEYVSST PQRLKLLDAY LLYILLTGAL QFLYCLLVGT FPFNSFLSGF 70 80 90 100 110 ISSVGSFILA VCLRIQINPQ NKSDFQGISP ERAFADFLFA NTILHLVVVN FIG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells." Nakashima T., Sekiguchi T., Kuraoka A., Fukushima K., Shibata Y., Komiyama S., Nishimoto T. Mol. Cell. Biol. 13:6367-6374(1993) [PubMed: 8413235] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D15059 mRNA. Translation: BAA03652.1. BC078613 mRNA. Translation: AAH78613.1.
PIR	B54437.
RefSeq	NP_001081227.1. NM_001087758.1.
UniGene	Xl.43587.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	397721.
KEGG	xla:397721.
Organism-specific databases
CTD	1603.
Xenbase	XB-GENE-922833. dad1.
Phylogenomic databases
HOVERGEN	HBG004452.
Family and domain databases
InterPro	IPR003038. DAD. [Graphical view]
KO	K12668.
PANTHER	PTHR10705. DAD. 1 hit.
Pfam	PF02109. DAD. 1 hit. [Graphical view]
PIRSF	PIRSF005588. DAD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DAD1_XENLA

Accession

Primary (citable) accession number: P46967
Secondary accession number(s): Q66KN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program