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P46965

- SPC1_YEAST

UniProt

P46965 - SPC1_YEAST

Protein

Signal peptidase complex subunit SPC1

Gene

SPC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Nonessential component of the signal peptidase complex (SPC), which catalyzes the cleavage of N-terminal signal sequences of proteins targeted to the endoplasmic reticulum. Signal peptide cleavage occurs during the translocation (cotranslationally or post-translationally) through the translocon pore into the endoplasmic reticulum. SPC3 is not required for signal peptidase activity.1 Publication

    GO - Molecular functioni

    1. peptidase activity Source: InterPro
    2. protein binding Source: IntAct

    GO - Biological processi

    1. protein targeting to ER Source: SGD
    2. signal peptide processing Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31655-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Signal peptidase complex subunit SPC1
    Alternative name(s):
    Microsomal signal peptidase subunit 1
    Gene namesi
    Name:SPC1
    Ordered Locus Names:YJR010C-A
    ORF Names:YJR010BW
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR010c-a.
    SGDiS000003770. SPC1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. signal peptidase complex Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 9493Signal peptidase complex subunit SPC1PRO_0000215162Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP46965.
    PaxDbiP46965.

    Expressioni

    Gene expression databases

    GenevestigatoriP46965.

    Interactioni

    Subunit structurei

    Component of the signal peptidase complex (SPC), which consists of SPC1, SPC2, SPC3 and SEC11. SPC associates with the translocon complex. SPC1 interacts with SBH1 and SEB2.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SEC11P153674EBI-17823,EBI-16513

    Protein-protein interaction databases

    BioGridi33767. 88 interactions.
    DIPiDIP-2770N.
    IntActiP46965. 17 interactions.
    MINTiMINT-482750.
    STRINGi4932.YJR010C-A.

    Structurei

    3D structure databases

    ProteinModelPortaliP46965.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 2827CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini50 – 501LumenalSequence Analysis
    Topological domaini72 – 9423CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei29 – 4921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei51 – 7121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the SPCS1 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG267017.
    HOGENOMiHOG000000815.
    KOiK12946.
    OMAiFAMICDA.
    OrthoDBiEOG74R240.

    Family and domain databases

    InterProiIPR009542. SPC12.
    [Graphical view]
    PANTHERiPTHR13202. PTHR13202. 1 hit.
    PfamiPF06645. SPC12. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P46965-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEILQDVQR KLVFPIDFPS QRKTEKFQQL SLMIGALVAC ILGFAQQSLK   50
    VLLTAYGISC VITLICVLPA YPWYNKQKLR WAQPKIEINV DQYD 94
    Length:94
    Mass (Da):10,819
    Last modified:November 1, 1995 - v1
    Checksum:i814D7C7A49F49D6D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U26257 Genomic DNA. Translation: AAC49366.1.
    Z49510 Genomic DNA. Translation: CAA89533.1.
    Z49511 Genomic DNA. Translation: CAA89535.1.
    AY557861 Genomic DNA. Translation: AAS56187.1.
    BK006943 Genomic DNA. Translation: DAA08802.1.
    PIRiS61934.
    RefSeqiNP_012544.1. NM_001181667.1.

    Genome annotation databases

    EnsemblFungiiYJR010C-A; YJR010C-A; YJR010C-A.
    GeneIDi853467.
    KEGGisce:YJR010C-A.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U26257 Genomic DNA. Translation: AAC49366.1 .
    Z49510 Genomic DNA. Translation: CAA89533.1 .
    Z49511 Genomic DNA. Translation: CAA89535.1 .
    AY557861 Genomic DNA. Translation: AAS56187.1 .
    BK006943 Genomic DNA. Translation: DAA08802.1 .
    PIRi S61934.
    RefSeqi NP_012544.1. NM_001181667.1.

    3D structure databases

    ProteinModelPortali P46965.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33767. 88 interactions.
    DIPi DIP-2770N.
    IntActi P46965. 17 interactions.
    MINTi MINT-482750.
    STRINGi 4932.YJR010C-A.

    Proteomic databases

    MaxQBi P46965.
    PaxDbi P46965.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR010C-A ; YJR010C-A ; YJR010C-A .
    GeneIDi 853467.
    KEGGi sce:YJR010C-A.

    Organism-specific databases

    CYGDi YJR010c-a.
    SGDi S000003770. SPC1.

    Phylogenomic databases

    eggNOGi NOG267017.
    HOGENOMi HOG000000815.
    KOi K12946.
    OMAi FAMICDA.
    OrthoDBi EOG74R240.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31655-MONOMER.

    Miscellaneous databases

    NextBioi 974059.
    PROi P46965.

    Gene expression databases

    Genevestigatori P46965.

    Family and domain databases

    InterProi IPR009542. SPC12.
    [Graphical view ]
    PANTHERi PTHR13202. PTHR13202. 1 hit.
    Pfami PF06645. SPC12. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The homologue of mammalian SPC12 is important for efficient signal peptidase activity in Saccharomyces cerevisiae."
      Fang H., Panzner S., Mullins C., Hartmann E., Green N.
      J. Biol. Chem. 271:16460-16465(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: AB320 / ATCC 37323.
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "The yeast SPC22/23 homolog Spc3p is essential for signal peptidase activity."
      Meyer H.A., Hartmann E.
      J. Biol. Chem. 272:13159-13164(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE SIGNAL PEPTIDASE COMPLEX.
    6. "Interactions between Spc2p and other components of the endoplasmic reticulum translocation sites of the yeast Saccharomyces cerevisiae."
      Antonin W., Meyer H.A., Hartmann E.
      J. Biol. Chem. 275:34068-34072(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SBH1 AND SEB2.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSPC1_YEAST
    AccessioniPrimary (citable) accession number: P46965
    Secondary accession number(s): D6VWI6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5550 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3