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P46965

- SPC1_YEAST

UniProt

P46965 - SPC1_YEAST

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Protein

Signal peptidase complex subunit SPC1

Gene

SPC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Nonessential component of the signal peptidase complex (SPC), which catalyzes the cleavage of N-terminal signal sequences of proteins targeted to the endoplasmic reticulum. Signal peptide cleavage occurs during the translocation (cotranslationally or post-translationally) through the translocon pore into the endoplasmic reticulum. SPC3 is not required for signal peptidase activity.1 Publication

GO - Molecular functioni

  1. peptidase activity Source: InterPro

GO - Biological processi

  1. protein targeting to ER Source: SGD
  2. signal peptide processing Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31655-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal peptidase complex subunit SPC1
Alternative name(s):
Microsomal signal peptidase subunit 1
Gene namesi
Name:SPC1
Ordered Locus Names:YJR010C-A
ORF Names:YJR010BW
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJR010c-a.
SGDiS000003770. SPC1.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. signal peptidase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 9493Signal peptidase complex subunit SPC1PRO_0000215162Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP46965.
PaxDbiP46965.

Expressioni

Gene expression databases

GenevestigatoriP46965.

Interactioni

Subunit structurei

Component of the signal peptidase complex (SPC), which consists of SPC1, SPC2, SPC3 and SEC11. SPC associates with the translocon complex. SPC1 interacts with SBH1 and SEB2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SEC11P153674EBI-17823,EBI-16513

Protein-protein interaction databases

BioGridi33767. 88 interactions.
DIPiDIP-2770N.
IntActiP46965. 17 interactions.
MINTiMINT-482750.
STRINGi4932.YJR010C-A.

Structurei

3D structure databases

ProteinModelPortaliP46965.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 2827CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini50 – 501LumenalSequence Analysis
Topological domaini72 – 9423CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei29 – 4921HelicalSequence AnalysisAdd
BLAST
Transmembranei51 – 7121HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the SPCS1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG267017.
HOGENOMiHOG000000815.
InParanoidiP46965.
KOiK12946.
OMAiFAMICDA.
OrthoDBiEOG74R240.

Family and domain databases

InterProiIPR009542. SPC12.
[Graphical view]
PANTHERiPTHR13202. PTHR13202. 1 hit.
PfamiPF06645. SPC12. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46965-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEILQDVQR KLVFPIDFPS QRKTEKFQQL SLMIGALVAC ILGFAQQSLK
60 70 80 90
VLLTAYGISC VITLICVLPA YPWYNKQKLR WAQPKIEINV DQYD
Length:94
Mass (Da):10,819
Last modified:November 1, 1995 - v1
Checksum:i814D7C7A49F49D6D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U26257 Genomic DNA. Translation: AAC49366.1.
Z49510 Genomic DNA. Translation: CAA89533.1.
Z49511 Genomic DNA. Translation: CAA89535.1.
AY557861 Genomic DNA. Translation: AAS56187.1.
BK006943 Genomic DNA. Translation: DAA08802.1.
PIRiS61934.
RefSeqiNP_012544.1. NM_001181667.1.

Genome annotation databases

EnsemblFungiiYJR010C-A; YJR010C-A; YJR010C-A.
GeneIDi853467.
KEGGisce:YJR010C-A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U26257 Genomic DNA. Translation: AAC49366.1 .
Z49510 Genomic DNA. Translation: CAA89533.1 .
Z49511 Genomic DNA. Translation: CAA89535.1 .
AY557861 Genomic DNA. Translation: AAS56187.1 .
BK006943 Genomic DNA. Translation: DAA08802.1 .
PIRi S61934.
RefSeqi NP_012544.1. NM_001181667.1.

3D structure databases

ProteinModelPortali P46965.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33767. 88 interactions.
DIPi DIP-2770N.
IntActi P46965. 17 interactions.
MINTi MINT-482750.
STRINGi 4932.YJR010C-A.

Proteomic databases

MaxQBi P46965.
PaxDbi P46965.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJR010C-A ; YJR010C-A ; YJR010C-A .
GeneIDi 853467.
KEGGi sce:YJR010C-A.

Organism-specific databases

CYGDi YJR010c-a.
SGDi S000003770. SPC1.

Phylogenomic databases

eggNOGi NOG267017.
HOGENOMi HOG000000815.
InParanoidi P46965.
KOi K12946.
OMAi FAMICDA.
OrthoDBi EOG74R240.

Enzyme and pathway databases

BioCyci YEAST:G3O-31655-MONOMER.

Miscellaneous databases

NextBioi 974059.
PROi P46965.

Gene expression databases

Genevestigatori P46965.

Family and domain databases

InterProi IPR009542. SPC12.
[Graphical view ]
PANTHERi PTHR13202. PTHR13202. 1 hit.
Pfami PF06645. SPC12. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The homologue of mammalian SPC12 is important for efficient signal peptidase activity in Saccharomyces cerevisiae."
    Fang H., Panzner S., Mullins C., Hartmann E., Green N.
    J. Biol. Chem. 271:16460-16465(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: AB320 / ATCC 37323.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "The yeast SPC22/23 homolog Spc3p is essential for signal peptidase activity."
    Meyer H.A., Hartmann E.
    J. Biol. Chem. 272:13159-13164(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE SIGNAL PEPTIDASE COMPLEX.
  6. "Interactions between Spc2p and other components of the endoplasmic reticulum translocation sites of the yeast Saccharomyces cerevisiae."
    Antonin W., Meyer H.A., Hartmann E.
    J. Biol. Chem. 275:34068-34072(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SBH1 AND SEB2.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSPC1_YEAST
AccessioniPrimary (citable) accession number: P46965
Secondary accession number(s): D6VWI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5550 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3