Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2

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Reviewed, UniProtKB/Swiss-Prot P46964 (OST2_YEAST)

Last modified November 3, 2009. Version 83. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2
      Short name=Oligosaccharyl transferase subunit OST2
    EC=2.4.1.119
Alternative name(s):
    Oligosaccharyl transferase subunit epsilon
    Oligosaccharyl transferase 16 kDa subunit

Gene names

Name:	OST2
Ordered Locus Names:	YOR103C
ORF Names:	YOR3211C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	130 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyl transferase (OST) complex, which apears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. WBP1, SWP1 and OST2 probably form a subcomplex. May interact directly with OST1, OST3, OST4, OST5, OST6, WBP1 and SWP1. Interacts with SEC61 and SSS1. Ref.10 Ref.11
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the DAD/OST2 family.

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Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation Ref.5 Inferred from physical interaction. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell oligosaccharyltransferase complex Ref.5 Ref.7 Inferred from physical interaction. Source: SGD
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Ref.5 Inferred from physical interaction. Source: SGD identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
itself		1	EBI-12673,EBI-12673
SBH1	P52870	1	EBI-12673,EBI-16410
SEC61	P32915	1	EBI-12673,EBI-16400

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.1

Chain

2 – 130

129

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2

PRO_0000124032

Regions

Topological domain

2 – 46

Cytoplasmic Potential

Transmembrane

47 – 67

Potential

Topological domain

68 – 71

Lumenal Potential

Transmembrane

72 – 92

Potential

Topological domain

93 – 109

Cytoplasmic Potential

Transmembrane

110 – 129

Potential

Topological domain

130

Lumenal Potential

Experimental info

Mutagenesis

S → P in OST2-3; ts; reduced activity.

Mutagenesis

E → G in OST2-3; ts; reduced activity.

Mutagenesis

K → M in OST2-1; ts; reduced activity.

Mutagenesis

D → V in OST2-2; ts; reduced activity.

Mutagenesis

Q → R in OST2-3; ts; reduced activity.

Mutagenesis

C → S in OST2-1; ts; reduced activity.

Mutagenesis

R → C in OST2-6; ts; reduced activity.

Mutagenesis

G → E in OST2-1; ts; reduced activity.

Mutagenesis

G → R in OST2-4; ts; reduced activity.

Mutagenesis

112

A → S in OST2-6; ts; reduced activity.

Mutagenesis

113

E → K in OST2-6; ts; reduced activity.

Mutagenesis

113

E → V in OST2-5; ts; reduced activity.

Mutagenesis

119

L → S in OST2-2; ts; reduced activity.

Mutagenesis

123

F → L in OST2-2; ts; reduced activity.

Mutagenesis

127

H → Y in OST2-1; ts; reduced activity.

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Sequences

Sequence

Length

Mass (Da)

Tools

P46964-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: B317E20D32B3573D
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FASTA

130

14,698

        10         20         30         40         50         60 
MAKAPKANTP KVTSTSSAVL TDFQETFKTS KRAYFAQIEK YPKLKLIDTF CFFLVLLGVI 

        70         80         90        100        110        120 
QCTFIILIRD NFPFNAFLAG FIICVGQFVL LMSLRLQLCN SFPGISKNRA FAEFIVASLI 

       130 
LHFVCLHFIN

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The essential OST2 gene encodes the 16-kD subunit of the yeast oligosaccharyltransferase, a highly conserved protein expressed in diverse eukaryotic organisms." Silberstein S., Collins P.G., Kelleher D.J., Gilmore R. J. Cell Biol. 131:371-383(1995) [PubMed: 7593165] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-28 AND 33-40.
[2]	"DNA sequencing and analysis of 130 kb from yeast chromosome XV." Voss H., Benes V., Andrade M.A., Valencia A., Rechmann S., Teodoru C., Schwager C., Paces V., Sander C., Ansorge W. Yeast 13:655-672(1997) [PubMed: 9200815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"The Saccharomyces cerevisiae oligosaccharyltransferase is a protein complex composed of Wbp1p, Swp1p, and four additional polypeptides." Kelleher D.J., Gilmore R. J. Biol. Chem. 269:12908-12917(1994) [PubMed: 8175708] [Abstract] Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
[5]	"The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p." Karaoglu D., Kelleher D.J., Gilmore R. J. Biol. Chem. 272:32513-32520(1997) [PubMed: 9405463] [Abstract] Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
[6]	"The oligosaccharyltransferase complex from yeast." Knauer R., Lehle L. Biochim. Biophys. Acta 1426:259-273(1999) [PubMed: 9878773] [Abstract] Cited for: REVIEW ON OLIGOSACCHARYL TRANSFERASE.
[7]	"Yeast oligosaccharyltransferase consists of two functionally distinct sub-complexes, specified by either the Ost3p or Ost6p subunit." Schwarz M., Knauer R., Lehle L. FEBS Lett. 579:6564-6568(2005) [PubMed: 16297388] [Abstract] Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[8]	"The 3.4-kDa Ost4 protein is required for the assembly of two distinct oligosaccharyltransferase complexes in yeast." Spirig U., Bodmer D., Wacker M., Burda P., Aebi M. Glycobiology 15:1396-1406(2005) [PubMed: 16096346] [Abstract] Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[9]	"Two oligosaccharyl transferase complexes exist in yeast and associate with two different translocons." Yan A., Lennarz W.J. Glycobiology 15:1407-1415(2005) [PubMed: 16096345] [Abstract] Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[10]	"Subunits of the translocon interact with components of the oligosaccharyl transferase complex." Chavan M., Yan A., Lennarz W.J. J. Biol. Chem. 280:22917-22924(2005) [PubMed: 15831493] [Abstract] Cited for: INTERACTION WITH SEC61 AND SSS1.
[11]	"Studies of yeast oligosaccharyl transferase subunits using the split-ubiquitin system: topological features and in vivo interactions." Yan A., Wu E., Lennarz W.J. Proc. Natl. Acad. Sci. U.S.A. 102:7121-7126(2005) [PubMed: 15886282] [Abstract] Cited for: TOPOLOGY, INTERACTION WITH OST1; OST3; OST4; OST5; OST6; WBP1 AND SWP1.
[12]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	U32307 Genomic DNA. Translation: AAC49086.1. X94335 Genomic DNA. Translation: CAA64024.1. Different initiation. Z75010 Genomic DNA. Translation: CAA99300.1. Different initiation.
PIR	S61662.
RefSeq	NP_014746.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2455N.
IntAct	P46964. 5 interactions.
STRING	P46964.
Proteomic databases
PeptideAtlas	P46964.
PRIDE	P46964.
Genome annotation databases
Ensembl	YOR103C; YOR103C; YOR103C; Saccharomyces cerevisiae. [Genome view]
GeneID	854270.
GenomeReviews	Gene locus YOR103C in contig Y13140_GR.
KEGG	sce:YOR103C.
NMPDR	fig\|4932.3.peg.5848.
Organism-specific databases
CYGD	YOR103c.
SGD	S000005629. OST2.
Phylogenomic databases
HOGENOM	P46964.
OMA	YCCLVGT.
Enzyme and pathway databases
BRENDA	2.4.1.119. 250.
Gene expression databases
ArrayExpress	P46964.
Genevestigator	P46964.
GermOnline	YOR103C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003038. DAD. [Graphical view]
PANTHER	PTHR10705. DAD. 1 hit.
Pfam	PF02109. DAD. 1 hit. [Graphical view]
PIRSF	PIRSF005588. DAD. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	976221.

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Entry information

Entry name

OST2_YEAST

Accession

Primary (citable) accession number: P46964

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents