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Protein

CTD kinase subunit beta

Gene

CTK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cyclin subunit of the CTDK-I complex, which hyperphosphorylates the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit. CTDK-I phosphorylates 'Ser-5' if the CTD substrate is not phosphorylated at 'Ser-5', but will phosphorylate 'Ser-2' of a CTD substrate if 'Ser-5' is already phosphorylated. CTDK-I is also more reactive toward substrates that are prephosphorylated at 'Ser-2' or 'Ser-5' compared with an unphosphorylated CTD substrate, therefore efficiently creating doubly phosphorylated CTD repeats. Involved in RNA polymerase II transcriptional elongation, and as part of the CTDK-I complex, pre-mRNA 3'-end processing and SET2 mediated H3K36 methylation. Together with CTK3, required for CTK1 CTD kinase activation. Required for DNA damage induced transcription. Involved in the adaptation to alternative carbon sources, including galactose, glycerol and ethanol, but not raffinose. Required for the integrity of the rDNA locus.6 Publications

Miscellaneous

Present with 1590 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCyclin
Biological processDNA damage, mRNA processing, Transcription

Enzyme and pathway databases

BioCyciYEAST:G3O-31484-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
CTD kinase subunit beta
Short name:
CTDK-I subunit beta
Alternative name(s):
CTD kinase 38 kDa subunit
CTD kinase subunit 2
Gene namesi
Name:CTK2
Ordered Locus Names:YJL006C
ORF Names:J1390
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL006C
SGDiS000003543 CTK2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Null mutants are viable, but grow more slowly than wild-type cells at 30 degrees Celsius. They are cold-sensitive, failing to grow at 12 degrees Celsius. They display flocculent growth in liquid media and they show abnormal cell morphologies, for example, a significant fraction of the cells are greatly enlarged. Deletion mutant is sensitive to the DNA synthesis inhibitor hydroxyurea (HU) and UV irradiation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805071 – 323CTD kinase subunit betaAdd BLAST323

Post-translational modificationi

Phosphorylated. Ubiquitinated. Phosphorylation and ubiquitination lead to degradation in growth-related way by the ubiquitin-proteasome pathway. Neither phosphorylation nor degradation requires association with CTK1.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP46962
PaxDbiP46962
PRIDEiP46962

PTM databases

iPTMnetiP46962

Interactioni

Subunit structurei

CTDK-I consists of three subunits, CTK1, CTK2 and CTK3 (also called alpha, beta and gamma). Interacts with CTK1. Heterodimerization with CTK3 is required to protect this subunit from degradation.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33750, 203 interactors
DIPiDIP-2105N
IntActiP46962, 6 interactors
MINTiP46962
STRINGi4932.YJL006C

Structurei

3D structure databases

ProteinModelPortaliP46962
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cyclin family.Curated

Phylogenomic databases

HOGENOMiHOG000112044
InParanoidiP46962
KOiK15564
OMAiFDIMSTP
OrthoDBiEOG092C3794

Family and domain databases

CDDicd00043 CYCLIN, 1 hit
InterProiView protein in InterPro
IPR013763 Cyclin-like
IPR036915 Cyclin-like_sf
IPR006671 Cyclin_N
PfamiView protein in Pfam
PF00134 Cyclin_N, 1 hit
SMARTiView protein in SMART
SM00385 CYCLIN, 1 hit
SUPFAMiSSF47954 SSF47954, 2 hits

Sequencei

Sequence statusi: Complete.

P46962-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSTFESQLF FSRPFLSKRQ IQRAQKNTIS DYRNYNQKKL AVFKFLSDLC
60 70 80 90 100
VQLKFPRKTL ETAVYFYQRY HLFNRFETEV CYTVATSCLT LGCKEVETIK
110 120 130 140 150
KTNDICTLSL RLRNVVKINT DILENFKKRV FQIELRILES CSFDYRVNNY
160 170 180 190 200
VHIDEYVIKI GRELSFDYKL CNLAWVIAYD ALKLETILVI PQHSIALAIL
210 220 230 240 250
KIAYELLDNK NWSSKRYSLF ETDEKSVNEA YFDIVNFYIN SFDMCDLQRH
260 270 280 290 300
LPADLLPIGV ERFMELKKNA GPESGLPQIP DHLLNADPYI TITRDNNVQE
310 320
RRYVLSLELI NGESSINSST RHA
Length:323
Mass (Da):37,905
Last modified:November 1, 1995 - v1
Checksum:i7C18FA672778D8FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30295 Genomic DNA Translation: AAC49077.1
Z49281 Genomic DNA Translation: CAA89296.1
BK006943 Genomic DNA Translation: DAA08785.1
PIRiS56777
RefSeqiNP_012528.1, NM_001181440.1

Genome annotation databases

EnsemblFungiiYJL006C; YJL006C; YJL006C
GeneIDi853450
KEGGisce:YJL006C

Similar proteinsi

Entry informationi

Entry nameiCTK2_YEAST
AccessioniPrimary (citable) accession number: P46962
Secondary accession number(s): D6VWG9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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