Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

CTD kinase subunit beta

Gene

CTK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cyclin subunit of the CTDK-I complex, which hyperphosphorylates the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit. CTDK-I phosphorylates 'Ser-5' if the CTD substrate is not phosphorylated at 'Ser-5', but will phosphorylate 'Ser-2' of a CTD substrate if 'Ser-5' is already phosphorylated. CTDK-I is also more reactive toward substrates that are prephosphorylated at 'Ser-2' or 'Ser-5' compared with an unphosphorylated CTD substrate, therefore efficiently creating doubly phosphorylated CTD repeats. Involved in RNA polymerase II transcriptional elongation, and as part of the CTDK-I complex, pre-mRNA 3'-end processing and SET2 mediated H3K36 methylation. Together with CTK3, required for CTK1 CTD kinase activation. Required for DNA damage induced transcription. Involved in the adaptation to alternative carbon sources, including galactose, glycerol and ethanol, but not raffinose. Required for the integrity of the rDNA locus.6 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Keywords - Biological processi

DNA damage, mRNA processing, Transcription

Enzyme and pathway databases

BioCyciYEAST:G3O-31484-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CTD kinase subunit beta
Short name:
CTDK-I subunit beta
Alternative name(s):
CTD kinase 38 kDa subunit
CTD kinase subunit 2
Gene namesi
Name:CTK2
Ordered Locus Names:YJL006C
ORF Names:J1390
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL006C.
SGDiS000003543. CTK2.

Subcellular locationi

GO - Cellular componenti

  • CTDK-1 complex Source: SGD
  • cytosol Source: SGD
  • nucleolus Source: SGD
  • nucleoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Null mutants are viable, but grow more slowly than wild-type cells at 30 degrees Celsius. They are cold-sensitive, failing to grow at 12 degrees Celsius. They display flocculent growth in liquid media and they show abnormal cell morphologies, for example, a significant fraction of the cells are greatly enlarged. Deletion mutant is sensitive to the DNA synthesis inhibitor hydroxyurea (HU) and UV irradiation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805071 – 323CTD kinase subunit betaAdd BLAST323

Post-translational modificationi

Phosphorylated. Ubiquitinated. Phosphorylation and ubiquitination lead to degradation in growth-related way by the ubiquitin-proteasome pathway. Neither phosphorylation nor degradation requires association with CTK1.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP46962.
PRIDEiP46962.

PTM databases

iPTMnetiP46962.

Interactioni

Subunit structurei

CTDK-I consists of three subunits, CTK1, CTK2 and CTK3 (also called alpha, beta and gamma). Interacts with CTK1. Heterodimerization with CTK3 is required to protect this subunit from degradation.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CTK1Q0395710EBI-5236,EBI-5230
CTK3P4696310EBI-5236,EBI-5241

Protein-protein interaction databases

BioGridi33750. 180 interactors.
DIPiDIP-2105N.
IntActiP46962. 6 interactors.
MINTiMINT-563465.

Structurei

3D structure databases

ProteinModelPortaliP46962.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cyclin family.Curated

Phylogenomic databases

HOGENOMiHOG000112044.
InParanoidiP46962.
KOiK15564.
OMAiFYINSFD.
OrthoDBiEOG092C3794.

Family and domain databases

Gene3Di1.10.472.10. 1 hit.
InterProiIPR013763. Cyclin-like.
IPR006671. Cyclin_N.
[Graphical view]
PfamiPF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.

Sequencei

Sequence statusi: Complete.

P46962-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSTFESQLF FSRPFLSKRQ IQRAQKNTIS DYRNYNQKKL AVFKFLSDLC
60 70 80 90 100
VQLKFPRKTL ETAVYFYQRY HLFNRFETEV CYTVATSCLT LGCKEVETIK
110 120 130 140 150
KTNDICTLSL RLRNVVKINT DILENFKKRV FQIELRILES CSFDYRVNNY
160 170 180 190 200
VHIDEYVIKI GRELSFDYKL CNLAWVIAYD ALKLETILVI PQHSIALAIL
210 220 230 240 250
KIAYELLDNK NWSSKRYSLF ETDEKSVNEA YFDIVNFYIN SFDMCDLQRH
260 270 280 290 300
LPADLLPIGV ERFMELKKNA GPESGLPQIP DHLLNADPYI TITRDNNVQE
310 320
RRYVLSLELI NGESSINSST RHA
Length:323
Mass (Da):37,905
Last modified:November 1, 1995 - v1
Checksum:i7C18FA672778D8FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30295 Genomic DNA. Translation: AAC49077.1.
Z49281 Genomic DNA. Translation: CAA89296.1.
BK006943 Genomic DNA. Translation: DAA08785.1.
PIRiS56777.
RefSeqiNP_012528.1. NM_001181440.1.

Genome annotation databases

EnsemblFungiiYJL006C; YJL006C; YJL006C.
GeneIDi853450.
KEGGisce:YJL006C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30295 Genomic DNA. Translation: AAC49077.1.
Z49281 Genomic DNA. Translation: CAA89296.1.
BK006943 Genomic DNA. Translation: DAA08785.1.
PIRiS56777.
RefSeqiNP_012528.1. NM_001181440.1.

3D structure databases

ProteinModelPortaliP46962.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33750. 180 interactors.
DIPiDIP-2105N.
IntActiP46962. 6 interactors.
MINTiMINT-563465.

PTM databases

iPTMnetiP46962.

Proteomic databases

MaxQBiP46962.
PRIDEiP46962.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL006C; YJL006C; YJL006C.
GeneIDi853450.
KEGGisce:YJL006C.

Organism-specific databases

EuPathDBiFungiDB:YJL006C.
SGDiS000003543. CTK2.

Phylogenomic databases

HOGENOMiHOG000112044.
InParanoidiP46962.
KOiK15564.
OMAiFYINSFD.
OrthoDBiEOG092C3794.

Enzyme and pathway databases

BioCyciYEAST:G3O-31484-MONOMER.

Miscellaneous databases

PROiP46962.

Family and domain databases

Gene3Di1.10.472.10. 1 hit.
InterProiIPR013763. Cyclin-like.
IPR006671. Cyclin_N.
[Graphical view]
PfamiPF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiCTK2_YEAST
AccessioniPrimary (citable) accession number: P46962
Secondary accession number(s): D6VWG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1590 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.