Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P46962

- CTK2_YEAST

UniProt

P46962 - CTK2_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

CTD kinase subunit beta

Gene

CTK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cyclin subunit of the CTDK-I complex, which hyperphosphorylates the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit. CTDK-I phosphorylates 'Ser-5' if the CTD substrate is not phosphorylated at 'Ser-5', but will phosphorylate 'Ser-2' of a CTD substrate if 'Ser-5' is already phosphorylated. CTDK-I is also more reactive toward substrates that are prephosphorylated at 'Ser-2' or 'Ser-5' compared with an unphosphorylated CTD substrate, therefore efficiently creating doubly phosphorylated CTD repeats. Involved in RNA polymerase II transcriptional elongation, and as part of the CTDK-I complex, pre-mRNA 3'-end processing and SET2 mediated H3K36 methylation. Together with CTK3, required for CTK1 CTD kinase activation. Required for DNA damage induced transcription. Involved in the adaptation to alternative carbon sources, including galactose, glycerol and ethanol, but not raffinose. Required for the integrity of the rDNA locus.6 Publications

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: UniProtKB-KW
  2. mRNA 3'-end processing Source: SGD
  3. positive regulation of DNA-templated transcription, elongation Source: SGD
  4. positive regulation of transcription from RNA polymerase I promoter Source: SGD
  5. positive regulation of translational fidelity Source: SGD
  6. protein phosphorylation Source: SGD
  7. regulation of cyclin-dependent protein serine/threonine kinase activity Source: InterPro
  8. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Keywords - Biological processi

DNA damage, mRNA processing, Transcription

Enzyme and pathway databases

BioCyciYEAST:G3O-31484-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CTD kinase subunit beta
Short name:
CTDK-I subunit beta
Alternative name(s):
CTD kinase 38 kDa subunit
CTD kinase subunit 2
Gene namesi
Name:CTK2
Ordered Locus Names:YJL006C
ORF Names:J1390
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL006c.
SGDiS000003543. CTK2.

Subcellular locationi

Nucleusnucleolus 2 Publications

GO - Cellular componenti

  1. cytosol Source: SGD
  2. nucleolus Source: SGD
  3. nucleoplasm Source: SGD
  4. nucleus Source: SGD
  5. trimeric positive transcription elongation factor complex b Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Null mutants are viable, but grow more slowly than wild-type cells at 30 degrees Celsius. They are cold-sensitive, failing to grow at 12 degrees Celsius. They display flocculent growth in liquid media and they show abnormal cell morphologies, for example, a significant fraction of the cells are greatly enlarged. Deletion mutant is sensitive to the DNA synthesis inhibitor hydroxyurea (HU) and UV irradiation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323CTD kinase subunit betaPRO_0000080507Add
BLAST

Post-translational modificationi

Phosphorylated. Ubiquitinated. Phosphorylation and ubiquitination lead to degradation in growth-related way by the ubiquitin-proteasome pathway. Neither phosphorylation nor degradation requires association with CTK1.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP46962.
PaxDbiP46962.

Expressioni

Gene expression databases

GenevestigatoriP46962.

Interactioni

Subunit structurei

CTDK-I consists of three subunits, CTK1, CTK2 and CTK3 (also called alpha, beta and gamma). Interacts with CTK1. Heterodimerization with CTK3 is required to protect this subunit from degradation.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CTK1Q0395710EBI-5236,EBI-5230
CTK3P4696310EBI-5236,EBI-5241

Protein-protein interaction databases

BioGridi33750. 180 interactions.
DIPiDIP-2105N.
IntActiP46962. 6 interactions.
MINTiMINT-563465.
STRINGi4932.YJL006C.

Structurei

3D structure databases

ProteinModelPortaliP46962.
SMRiP46962. Positions 44-215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cyclin family.Curated

Phylogenomic databases

eggNOGiCOG5333.
HOGENOMiHOG000112044.
InParanoidiP46962.
KOiK15564.
OMAiIRERRYV.
OrthoDBiEOG7Z95X4.

Family and domain databases

Gene3Di1.10.472.10. 1 hit.
InterProiIPR013763. Cyclin-like.
IPR015429. Cyclin_C/H/T/L.
IPR006671. Cyclin_N.
[Graphical view]
PANTHERiPTHR10026. PTHR10026. 1 hit.
PfamiPF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.

Sequencei

Sequence statusi: Complete.

P46962-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPSTFESQLF FSRPFLSKRQ IQRAQKNTIS DYRNYNQKKL AVFKFLSDLC
60 70 80 90 100
VQLKFPRKTL ETAVYFYQRY HLFNRFETEV CYTVATSCLT LGCKEVETIK
110 120 130 140 150
KTNDICTLSL RLRNVVKINT DILENFKKRV FQIELRILES CSFDYRVNNY
160 170 180 190 200
VHIDEYVIKI GRELSFDYKL CNLAWVIAYD ALKLETILVI PQHSIALAIL
210 220 230 240 250
KIAYELLDNK NWSSKRYSLF ETDEKSVNEA YFDIVNFYIN SFDMCDLQRH
260 270 280 290 300
LPADLLPIGV ERFMELKKNA GPESGLPQIP DHLLNADPYI TITRDNNVQE
310 320
RRYVLSLELI NGESSINSST RHA
Length:323
Mass (Da):37,905
Last modified:November 1, 1995 - v1
Checksum:i7C18FA672778D8FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30295 Genomic DNA. Translation: AAC49077.1.
Z49281 Genomic DNA. Translation: CAA89296.1.
BK006943 Genomic DNA. Translation: DAA08785.1.
PIRiS56777.
RefSeqiNP_012528.1. NM_001181440.1.

Genome annotation databases

EnsemblFungiiYJL006C; YJL006C; YJL006C.
GeneIDi853450.
KEGGisce:YJL006C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30295 Genomic DNA. Translation: AAC49077.1 .
Z49281 Genomic DNA. Translation: CAA89296.1 .
BK006943 Genomic DNA. Translation: DAA08785.1 .
PIRi S56777.
RefSeqi NP_012528.1. NM_001181440.1.

3D structure databases

ProteinModelPortali P46962.
SMRi P46962. Positions 44-215.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33750. 180 interactions.
DIPi DIP-2105N.
IntActi P46962. 6 interactions.
MINTi MINT-563465.
STRINGi 4932.YJL006C.

Proteomic databases

MaxQBi P46962.
PaxDbi P46962.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL006C ; YJL006C ; YJL006C .
GeneIDi 853450.
KEGGi sce:YJL006C.

Organism-specific databases

CYGDi YJL006c.
SGDi S000003543. CTK2.

Phylogenomic databases

eggNOGi COG5333.
HOGENOMi HOG000112044.
InParanoidi P46962.
KOi K15564.
OMAi IRERRYV.
OrthoDBi EOG7Z95X4.

Enzyme and pathway databases

BioCyci YEAST:G3O-31484-MONOMER.

Miscellaneous databases

NextBioi 974009.
PROi P46962.

Gene expression databases

Genevestigatori P46962.

Family and domain databases

Gene3Di 1.10.472.10. 1 hit.
InterProi IPR013763. Cyclin-like.
IPR015429. Cyclin_C/H/T/L.
IPR006671. Cyclin_N.
[Graphical view ]
PANTHERi PTHR10026. PTHR10026. 1 hit.
Pfami PF00134. Cyclin_N. 1 hit.
[Graphical view ]
SMARTi SM00385. CYCLIN. 1 hit.
[Graphical view ]
SUPFAMi SSF47954. SSF47954. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast carboxyl-terminal repeat domain kinase CTDK-I is a divergent cyclin-cyclin-dependent kinase complex."
    Sterner D.E., Lee J.M., Hardin S.E., Greenleaf A.L.
    Mol. Cell. Biol. 15:5716-5724(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 217-225, CTD KINASE ACTIVITY, SUBUNIT, DISRUPTION PHENOTYPE.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Modulation of RNA polymerase II elongation efficiency by C-terminal heptapeptide repeat domain kinase I."
    Lee J.M., Greenleaf A.L.
    J. Biol. Chem. 272:10990-10993(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN RNA POLYMERASE II TRANSCRIPTION.
  5. "The yeast C-type cyclin Ctk2p is phosphorylated and rapidly degraded by the ubiquitin-proteasome pathway."
    Hautbergue G., Goguel V.
    Mol. Cell. Biol. 19:2527-2534(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION, UBIQUITINATION.
  6. "Activation of the cyclin-dependent kinase CTDK-I requires the heterodimerization of two unstable subunits."
    Hautbergue G., Goguel V.
    J. Biol. Chem. 276:8005-8013(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CTK1 ACTIVATION, INTERACTION WITH CTK1 AND CTK3.
  7. "Budding yeast CTDK-I is required for DNA damage-induced transcription."
    Ostapenko D., Solomon M.J.
    Eukaryot. Cell 2:274-283(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  8. "Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast."
    Xiao T., Hall H., Kizer K.O., Shibata Y., Hall M.C., Borchers C.H., Strahl B.D.
    Genes Dev. 17:654-663(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN H3 METHYLATION.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "C-terminal repeat domain kinase I phosphorylates Ser2 and Ser5 of RNA polymerase II C-terminal domain repeats."
    Jones J.C., Phatnani H.P., Haystead T.A., MacDonald J.A., Alam S.M., Greenleaf A.L.
    J. Biol. Chem. 279:24957-24964(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE CTDK-I COMPLEX.
  12. Cited for: SUBCELLULAR LOCATION.
  13. "Glucose deprivation mediates interaction between CTDK-I and Snf1 in Saccharomyces cerevisiae."
    Van Driessche B., Coddens S., Van Mullem V., Vandenhaute J.
    FEBS Lett. 579:5318-5324(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN ADAPTATION TO ALTERNATIVE CARBON SOURCES.
  14. "CTD kinase I is required for the integrity of the rDNA tandem array."
    Grenetier S., Bouchoux C., Goguel V.
    Nucleic Acids Res. 34:4996-5006(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN THE INTEGRITY OF RDNA.

Entry informationi

Entry nameiCTK2_YEAST
AccessioniPrimary (citable) accession number: P46962
Secondary accession number(s): D6VWG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1590 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3