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P46962

- CTK2_YEAST

UniProt

P46962 - CTK2_YEAST

Protein

CTD kinase subunit beta

Gene

CTK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Cyclin subunit of the CTDK-I complex, which hyperphosphorylates the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit. CTDK-I phosphorylates 'Ser-5' if the CTD substrate is not phosphorylated at 'Ser-5', but will phosphorylate 'Ser-2' of a CTD substrate if 'Ser-5' is already phosphorylated. CTDK-I is also more reactive toward substrates that are prephosphorylated at 'Ser-2' or 'Ser-5' compared with an unphosphorylated CTD substrate, therefore efficiently creating doubly phosphorylated CTD repeats. Involved in RNA polymerase II transcriptional elongation, and as part of the CTDK-I complex, pre-mRNA 3'-end processing and SET2 mediated H3K36 methylation. Together with CTK3, required for CTK1 CTD kinase activation. Required for DNA damage induced transcription. Involved in the adaptation to alternative carbon sources, including galactose, glycerol and ethanol, but not raffinose. Required for the integrity of the rDNA locus.6 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: UniProtKB-KW
    2. mRNA 3'-end processing Source: SGD
    3. positive regulation of DNA-templated transcription, elongation Source: SGD
    4. positive regulation of transcription from RNA polymerase I promoter Source: SGD
    5. positive regulation of translational fidelity Source: SGD
    6. protein phosphorylation Source: SGD
    7. regulation of cyclin-dependent protein serine/threonine kinase activity Source: InterPro
    8. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Cyclin

    Keywords - Biological processi

    DNA damage, mRNA processing, Transcription

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31484-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CTD kinase subunit beta
    Short name:
    CTDK-I subunit beta
    Alternative name(s):
    CTD kinase 38 kDa subunit
    CTD kinase subunit 2
    Gene namesi
    Name:CTK2
    Ordered Locus Names:YJL006C
    ORF Names:J1390
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL006c.
    SGDiS000003543. CTK2.

    Subcellular locationi

    Nucleusnucleolus 2 Publications

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. nucleolus Source: SGD
    3. nucleoplasm Source: SGD
    4. nucleus Source: SGD
    5. trimeric positive transcription elongation factor complex b Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Null mutants are viable, but grow more slowly than wild-type cells at 30 degrees Celsius. They are cold-sensitive, failing to grow at 12 degrees Celsius. They display flocculent growth in liquid media and they show abnormal cell morphologies, for example, a significant fraction of the cells are greatly enlarged. Deletion mutant is sensitive to the DNA synthesis inhibitor hydroxyurea (HU) and UV irradiation.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 323323CTD kinase subunit betaPRO_0000080507Add
    BLAST

    Post-translational modificationi

    Phosphorylated. Ubiquitinated. Phosphorylation and ubiquitination lead to degradation in growth-related way by the ubiquitin-proteasome pathway. Neither phosphorylation nor degradation requires association with CTK1.1 Publication

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiP46962.
    PaxDbiP46962.

    Expressioni

    Gene expression databases

    GenevestigatoriP46962.

    Interactioni

    Subunit structurei

    CTDK-I consists of three subunits, CTK1, CTK2 and CTK3 (also called alpha, beta and gamma). Interacts with CTK1. Heterodimerization with CTK3 is required to protect this subunit from degradation.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CTK1Q0395710EBI-5236,EBI-5230
    CTK3P4696310EBI-5236,EBI-5241

    Protein-protein interaction databases

    BioGridi33750. 179 interactions.
    DIPiDIP-2105N.
    IntActiP46962. 6 interactions.
    MINTiMINT-563465.
    STRINGi4932.YJL006C.

    Structurei

    3D structure databases

    ProteinModelPortaliP46962.
    SMRiP46962. Positions 44-215.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cyclin family.Curated

    Phylogenomic databases

    eggNOGiCOG5333.
    HOGENOMiHOG000112044.
    KOiK15564.
    OMAiIRERRYV.
    OrthoDBiEOG7Z95X4.

    Family and domain databases

    Gene3Di1.10.472.10. 1 hit.
    InterProiIPR013763. Cyclin-like.
    IPR015429. Cyclin_C/H/T/L.
    IPR006671. Cyclin_N.
    [Graphical view]
    PANTHERiPTHR10026. PTHR10026. 1 hit.
    PfamiPF00134. Cyclin_N. 1 hit.
    [Graphical view]
    SMARTiSM00385. CYCLIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF47954. SSF47954. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P46962-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSTFESQLF FSRPFLSKRQ IQRAQKNTIS DYRNYNQKKL AVFKFLSDLC    50
    VQLKFPRKTL ETAVYFYQRY HLFNRFETEV CYTVATSCLT LGCKEVETIK 100
    KTNDICTLSL RLRNVVKINT DILENFKKRV FQIELRILES CSFDYRVNNY 150
    VHIDEYVIKI GRELSFDYKL CNLAWVIAYD ALKLETILVI PQHSIALAIL 200
    KIAYELLDNK NWSSKRYSLF ETDEKSVNEA YFDIVNFYIN SFDMCDLQRH 250
    LPADLLPIGV ERFMELKKNA GPESGLPQIP DHLLNADPYI TITRDNNVQE 300
    RRYVLSLELI NGESSINSST RHA 323
    Length:323
    Mass (Da):37,905
    Last modified:November 1, 1995 - v1
    Checksum:i7C18FA672778D8FD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U30295 Genomic DNA. Translation: AAC49077.1.
    Z49281 Genomic DNA. Translation: CAA89296.1.
    BK006943 Genomic DNA. Translation: DAA08785.1.
    PIRiS56777.
    RefSeqiNP_012528.1. NM_001181440.1.

    Genome annotation databases

    EnsemblFungiiYJL006C; YJL006C; YJL006C.
    GeneIDi853450.
    KEGGisce:YJL006C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U30295 Genomic DNA. Translation: AAC49077.1 .
    Z49281 Genomic DNA. Translation: CAA89296.1 .
    BK006943 Genomic DNA. Translation: DAA08785.1 .
    PIRi S56777.
    RefSeqi NP_012528.1. NM_001181440.1.

    3D structure databases

    ProteinModelPortali P46962.
    SMRi P46962. Positions 44-215.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33750. 179 interactions.
    DIPi DIP-2105N.
    IntActi P46962. 6 interactions.
    MINTi MINT-563465.
    STRINGi 4932.YJL006C.

    Proteomic databases

    MaxQBi P46962.
    PaxDbi P46962.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL006C ; YJL006C ; YJL006C .
    GeneIDi 853450.
    KEGGi sce:YJL006C.

    Organism-specific databases

    CYGDi YJL006c.
    SGDi S000003543. CTK2.

    Phylogenomic databases

    eggNOGi COG5333.
    HOGENOMi HOG000112044.
    KOi K15564.
    OMAi IRERRYV.
    OrthoDBi EOG7Z95X4.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31484-MONOMER.

    Miscellaneous databases

    NextBioi 974009.
    PROi P46962.

    Gene expression databases

    Genevestigatori P46962.

    Family and domain databases

    Gene3Di 1.10.472.10. 1 hit.
    InterProi IPR013763. Cyclin-like.
    IPR015429. Cyclin_C/H/T/L.
    IPR006671. Cyclin_N.
    [Graphical view ]
    PANTHERi PTHR10026. PTHR10026. 1 hit.
    Pfami PF00134. Cyclin_N. 1 hit.
    [Graphical view ]
    SMARTi SM00385. CYCLIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47954. SSF47954. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The yeast carboxyl-terminal repeat domain kinase CTDK-I is a divergent cyclin-cyclin-dependent kinase complex."
      Sterner D.E., Lee J.M., Hardin S.E., Greenleaf A.L.
      Mol. Cell. Biol. 15:5716-5724(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 217-225, CTD KINASE ACTIVITY, SUBUNIT, DISRUPTION PHENOTYPE.
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Modulation of RNA polymerase II elongation efficiency by C-terminal heptapeptide repeat domain kinase I."
      Lee J.M., Greenleaf A.L.
      J. Biol. Chem. 272:10990-10993(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN RNA POLYMERASE II TRANSCRIPTION.
    5. "The yeast C-type cyclin Ctk2p is phosphorylated and rapidly degraded by the ubiquitin-proteasome pathway."
      Hautbergue G., Goguel V.
      Mol. Cell. Biol. 19:2527-2534(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION, UBIQUITINATION.
    6. "Activation of the cyclin-dependent kinase CTDK-I requires the heterodimerization of two unstable subunits."
      Hautbergue G., Goguel V.
      J. Biol. Chem. 276:8005-8013(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CTK1 ACTIVATION, INTERACTION WITH CTK1 AND CTK3.
    7. "Budding yeast CTDK-I is required for DNA damage-induced transcription."
      Ostapenko D., Solomon M.J.
      Eukaryot. Cell 2:274-283(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    8. "Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast."
      Xiao T., Hall H., Kizer K.O., Shibata Y., Hall M.C., Borchers C.H., Strahl B.D.
      Genes Dev. 17:654-663(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN H3 METHYLATION.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "C-terminal repeat domain kinase I phosphorylates Ser2 and Ser5 of RNA polymerase II C-terminal domain repeats."
      Jones J.C., Phatnani H.P., Haystead T.A., MacDonald J.A., Alam S.M., Greenleaf A.L.
      J. Biol. Chem. 279:24957-24964(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE CTDK-I COMPLEX.
    12. Cited for: SUBCELLULAR LOCATION.
    13. "Glucose deprivation mediates interaction between CTDK-I and Snf1 in Saccharomyces cerevisiae."
      Van Driessche B., Coddens S., Van Mullem V., Vandenhaute J.
      FEBS Lett. 579:5318-5324(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN ADAPTATION TO ALTERNATIVE CARBON SOURCES.
    14. "CTD kinase I is required for the integrity of the rDNA tandem array."
      Grenetier S., Bouchoux C., Goguel V.
      Nucleic Acids Res. 34:4996-5006(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN THE INTEGRITY OF RDNA.

    Entry informationi

    Entry nameiCTK2_YEAST
    AccessioniPrimary (citable) accession number: P46962
    Secondary accession number(s): D6VWG9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1590 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3