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Protein

tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61

Gene

GCD14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. GCD14 is also required for repression of GCN4 mRNA translation by the upstream open reading frames (uORFs) under conditions of amino acid sufficiency.3 Publications

Catalytic activityi

S-adenosyl-L-methionine + adenine(58) in tRNA = S-adenosyl-L-homocysteine + N(1)-methyladenine(58) in tRNA.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei121S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei139S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei168S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei203S-adenosyl-L-methioninePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • tRNA methylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Repressor, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:G3O-31576-MONOMER.
YEAST:G3O-31576-MONOMER.
BRENDAi2.1.1.220. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 (EC:2.1.1.220)
Alternative name(s):
General control non-derepressible protein 14
Short name:
Protein GCD14
tRNA(m1A58)-methyltransferase subunit TRM61
Short name:
tRNA(m1A58)MTase subunit TRM61
Gene namesi
Name:GCD14
Synonyms:TRM61
Ordered Locus Names:YJL125C
ORF Names:J0710
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL125C.
SGDiS000003661. GCD14.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
  • tRNA (m1A) methyltransferase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000874371 – 383tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61Add BLAST383

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei302PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP46959.
PRIDEiP46959.

PTM databases

iPTMnetiP46959.

Interactioni

Subunit structurei

tRNA (adenine-N(1)-)-methyltransferase is a heterodimer of TRM6/GCD10 and TRM61/GCD14.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GCD10P418143EBI-7416,EBI-8995

Protein-protein interaction databases

BioGridi33631. 74 interactors.
DIPiDIP-5421N.
IntActiP46959. 17 interactors.
MINTiMINT-540985.

Structurei

Secondary structure

1383
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi18 – 24Combined sources7
Beta strandi27 – 32Combined sources6
Beta strandi38 – 41Combined sources4
Beta strandi44 – 47Combined sources4
Helixi48 – 50Combined sources3
Turni51 – 53Combined sources3
Beta strandi58 – 62Combined sources5
Beta strandi71 – 76Combined sources6
Helixi80 – 86Combined sources7
Helixi96 – 105Combined sources10
Beta strandi113 – 117Combined sources5
Helixi123 – 131Combined sources9
Beta strandi133 – 141Combined sources9
Helixi142 – 154Combined sources13
Turni158 – 161Combined sources4
Beta strandi162 – 166Combined sources5
Helixi169 – 172Combined sources4
Beta strandi180 – 182Combined sources3
Beta strandi197 – 202Combined sources6
Helixi207 – 209Combined sources3
Helixi211 – 216Combined sources6
Beta strandi220 – 222Combined sources3
Beta strandi224 – 232Combined sources9
Helixi233 – 245Combined sources13
Beta strandi249 – 267Combined sources19
Helixi271 – 283Combined sources13
Beta strandi341 – 347Combined sources7
Beta strandi357 – 363Combined sources7
Helixi371 – 375Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5EQJX-ray2.20B1-383[»]
5ERGX-ray2.20B1-383[»]
ProteinModelPortaliP46959.
SMRiP46959.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM61 family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00510000048063.
HOGENOMiHOG000173282.
InParanoidiP46959.
KOiK07442.
OMAiIMQRLNC.
OrthoDBiEOG092C4FRI.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR029063. SAM-dependent_MTases.
IPR014816. tRNA_MeTrfase_Gcd14.
[Graphical view]
PfamiPF08704. GCD14. 1 hit.
[Graphical view]
PIRSFiPIRSF017269. GCD14. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51620. SAM_TRM61. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46959-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTNCFSGYK DLIKEGDLTL IWVSRDNIKP VRMHSEEVFN TRYGSFPHKD
60 70 80 90 100
IIGKPYGSQI AIRTKGSNKF AFVHVLQPTP ELWTLSLPHR TQIVYTPDSS
110 120 130 140 150
YIMQRLNCSP HSRVIEAGTG SGSFSHAFAR SVGHLFSFEF HHIRYEQALE
160 170 180 190 200
EFKEHGLIDD NVTITHRDVC QGGFLIKKGD TTSYEFGNNE TAASLNANVV
210 220 230 240 250
FLDLPAPWDA IPHLDSVISV DEKVGLCCFS PCIEQVDKTL DVLEKYGWTD
260 270 280 290 300
VEMVEIQGRQ YESRRQMVRS LNDALERLRD IKRHKLQGVE RRKRMFNNTI
310 320 330 340 350
DSNDEKVGKR NEDGVPLTEK AKFNPFGKGS RIKEGDSNYK WKEVTKMEAE
360 370 380
IKSHTSYLTF AFKVVNRSRD DEKVNEILRS TEK
Length:383
Mass (Da):43,920
Last modified:November 1, 1995 - v1
Checksum:iA8402DF77A628FD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54149 Genomic DNA. Translation: CAA90863.1.
Z49400 Genomic DNA. Translation: CAA89420.1.
BK006943 Genomic DNA. Translation: DAA08677.1.
PIRiS56906.
RefSeqiNP_012410.1. NM_001181558.1.

Genome annotation databases

EnsemblFungiiYJL125C; YJL125C; YJL125C.
GeneIDi853317.
KEGGisce:YJL125C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54149 Genomic DNA. Translation: CAA90863.1.
Z49400 Genomic DNA. Translation: CAA89420.1.
BK006943 Genomic DNA. Translation: DAA08677.1.
PIRiS56906.
RefSeqiNP_012410.1. NM_001181558.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5EQJX-ray2.20B1-383[»]
5ERGX-ray2.20B1-383[»]
ProteinModelPortaliP46959.
SMRiP46959.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33631. 74 interactors.
DIPiDIP-5421N.
IntActiP46959. 17 interactors.
MINTiMINT-540985.

PTM databases

iPTMnetiP46959.

Proteomic databases

MaxQBiP46959.
PRIDEiP46959.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL125C; YJL125C; YJL125C.
GeneIDi853317.
KEGGisce:YJL125C.

Organism-specific databases

EuPathDBiFungiDB:YJL125C.
SGDiS000003661. GCD14.

Phylogenomic databases

GeneTreeiENSGT00510000048063.
HOGENOMiHOG000173282.
InParanoidiP46959.
KOiK07442.
OMAiIMQRLNC.
OrthoDBiEOG092C4FRI.

Enzyme and pathway databases

BioCyciMetaCyc:G3O-31576-MONOMER.
YEAST:G3O-31576-MONOMER.
BRENDAi2.1.1.220. 984.

Miscellaneous databases

PROiP46959.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR029063. SAM-dependent_MTases.
IPR014816. tRNA_MeTrfase_Gcd14.
[Graphical view]
PfamiPF08704. GCD14. 1 hit.
[Graphical view]
PIRSFiPIRSF017269. GCD14. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51620. SAM_TRM61. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRM61_YEAST
AccessioniPrimary (citable) accession number: P46959
Secondary accession number(s): D6VW61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7220 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.