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P46959

- TRM61_YEAST

UniProt

P46959 - TRM61_YEAST

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Protein

tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61

Gene

GCD14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. GCD14 is also required for repression of GCN4 mRNA translation by the upstream open reading frames (uORFs) under conditions of amino acid sufficiency.3 Publications

Catalytic activityi

S-adenosyl-L-methionine + adenine(58) in tRNA = S-adenosyl-L-homocysteine + N(1)-methyladenine(58) in tRNA.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei121 – 1211S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei139 – 1391S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei168 – 1681S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei203 – 2031S-adenosyl-L-methioninePROSITE-ProRule annotation

GO - Molecular functioni

  1. tRNA (adenine-N1-)-methyltransferase activity Source: InterPro

GO - Biological processi

  1. tRNA methylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Repressor, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16691.
YEAST:G3O-31576-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 (EC:2.1.1.220)
Alternative name(s):
General control non-derepressible protein 14
Short name:
Protein GCD14
tRNA(m1A58)-methyltransferase subunit TRM61
Short name:
tRNA(m1A58)MTase subunit TRM61
Gene namesi
Name:GCD14
Synonyms:TRM61
Ordered Locus Names:YJL125C
ORF Names:J0710
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL125c.
SGDiS000003661. GCD14.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: SGD
  2. tRNA (m1A) methyltransferase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 383383tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61PRO_0000087437Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei302 – 3021Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP46959.
PaxDbiP46959.
PeptideAtlasiP46959.

Expressioni

Gene expression databases

GenevestigatoriP46959.

Interactioni

Subunit structurei

tRNA (adenine-N(1)-)-methyltransferase is a heterodimer of TRM6/GCD10 and TRM61/GCD14.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GCD10P418143EBI-7416,EBI-8995

Protein-protein interaction databases

BioGridi33631. 73 interactions.
DIPiDIP-5421N.
IntActiP46959. 17 interactions.
MINTiMINT-540985.
STRINGi4932.YJL125C.

Structurei

3D structure databases

ProteinModelPortaliP46959.
SMRiP46959. Positions 11-272.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM61 family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG2519.
GeneTreeiENSGT00510000048063.
HOGENOMiHOG000173282.
InParanoidiP46959.
KOiK07442.
OMAiVEIQGRQ.
OrthoDBiEOG7B8SCW.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR014816. tRNA_MeTrfase_Gcd14.
[Graphical view]
PfamiPF08704. GCD14. 1 hit.
[Graphical view]
PIRSFiPIRSF017269. GCD14. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51620. SAM_TRM61. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46959-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTNCFSGYK DLIKEGDLTL IWVSRDNIKP VRMHSEEVFN TRYGSFPHKD
60 70 80 90 100
IIGKPYGSQI AIRTKGSNKF AFVHVLQPTP ELWTLSLPHR TQIVYTPDSS
110 120 130 140 150
YIMQRLNCSP HSRVIEAGTG SGSFSHAFAR SVGHLFSFEF HHIRYEQALE
160 170 180 190 200
EFKEHGLIDD NVTITHRDVC QGGFLIKKGD TTSYEFGNNE TAASLNANVV
210 220 230 240 250
FLDLPAPWDA IPHLDSVISV DEKVGLCCFS PCIEQVDKTL DVLEKYGWTD
260 270 280 290 300
VEMVEIQGRQ YESRRQMVRS LNDALERLRD IKRHKLQGVE RRKRMFNNTI
310 320 330 340 350
DSNDEKVGKR NEDGVPLTEK AKFNPFGKGS RIKEGDSNYK WKEVTKMEAE
360 370 380
IKSHTSYLTF AFKVVNRSRD DEKVNEILRS TEK
Length:383
Mass (Da):43,920
Last modified:November 1, 1995 - v1
Checksum:iA8402DF77A628FD3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z54149 Genomic DNA. Translation: CAA90863.1.
Z49400 Genomic DNA. Translation: CAA89420.1.
BK006943 Genomic DNA. Translation: DAA08677.1.
PIRiS56906.
RefSeqiNP_012410.1. NM_001181558.1.

Genome annotation databases

EnsemblFungiiYJL125C; YJL125C; YJL125C.
GeneIDi853317.
KEGGisce:YJL125C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z54149 Genomic DNA. Translation: CAA90863.1 .
Z49400 Genomic DNA. Translation: CAA89420.1 .
BK006943 Genomic DNA. Translation: DAA08677.1 .
PIRi S56906.
RefSeqi NP_012410.1. NM_001181558.1.

3D structure databases

ProteinModelPortali P46959.
SMRi P46959. Positions 11-272.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33631. 73 interactions.
DIPi DIP-5421N.
IntActi P46959. 17 interactions.
MINTi MINT-540985.
STRINGi 4932.YJL125C.

Proteomic databases

MaxQBi P46959.
PaxDbi P46959.
PeptideAtlasi P46959.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL125C ; YJL125C ; YJL125C .
GeneIDi 853317.
KEGGi sce:YJL125C.

Organism-specific databases

CYGDi YJL125c.
SGDi S000003661. GCD14.

Phylogenomic databases

eggNOGi COG2519.
GeneTreei ENSGT00510000048063.
HOGENOMi HOG000173282.
InParanoidi P46959.
KOi K07442.
OMAi VEIQGRQ.
OrthoDBi EOG7B8SCW.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16691.
YEAST:G3O-31576-MONOMER.

Miscellaneous databases

NextBioi 973663.
PROi P46959.

Gene expression databases

Genevestigatori P46959.

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR014816. tRNA_MeTrfase_Gcd14.
[Graphical view ]
Pfami PF08704. GCD14. 1 hit.
[Graphical view ]
PIRSFi PIRSF017269. GCD14. 1 hit.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS51620. SAM_TRM61. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cuesta R.
    Thesis (1996), University of Salamanca, Spain
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / GRF88.
  2. "Sequencing analysis of a 40.2 kb fragment of yeast chromosome X reveals 19 open reading frames including URA2 (5' end), TRK1, PBS2, SPT10, GCD14, RPE1, PHO86, NCA3, ASF1, CCT7, GZF3, two tRNA genes, three remnant delta elements and a Ty4 transposon."
    Cziepluch C., Kordes E., Pujol A., Jauniaux J.-C.
    Yeast 12:1471-1474(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA."
    Anderson J., Phan L., Cuesta R., Carlson B.A., Pak M., Asano K., Bjoerk G.R., Tamame M., Hinnebusch A.G.
    Genes Dev. 12:3650-3662(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A TRNA METHYLTRANSFERASE, SUBUNIT, SUBCELLULAR LOCATION.
  6. "Identification of GCD14 and GCD15, novel genes required for translational repression of GCN4 mRNA in Saccharomyces cerevisiae."
    Cuesta R., Hinnebusch A.G., Tamame M.
    Genetics 148:1007-1020(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TRANSLATIONAL REPRESSION.
  7. "The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae."
    Anderson J., Phan L., Hinnebusch A.G.
    Proc. Natl. Acad. Sci. U.S.A. 97:5173-5178(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A TRNA METHYLTRANSFERASE.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTRM61_YEAST
AccessioniPrimary (citable) accession number: P46959
Secondary accession number(s): D6VW61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7220 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3