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P46959

- TRM61_YEAST

UniProt

P46959 - TRM61_YEAST

Protein

tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61

Gene

GCD14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. GCD14 is also required for repression of GCN4 mRNA translation by the upstream open reading frames (uORFs) under conditions of amino acid sufficiency.3 Publications

    Catalytic activityi

    S-adenosyl-L-methionine + adenine(58) in tRNA = S-adenosyl-L-homocysteine + N(1)-methyladenine(58) in tRNA.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei121 – 1211S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei139 – 1391S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei168 – 1681S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei203 – 2031S-adenosyl-L-methioninePROSITE-ProRule annotation

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. tRNA (adenine-N1-)-methyltransferase activity Source: InterPro

    GO - Biological processi

    1. tRNA methylation Source: SGD

    Keywords - Molecular functioni

    Methyltransferase, Repressor, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16691.
    YEAST:G3O-31576-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 (EC:2.1.1.220)
    Alternative name(s):
    General control non-derepressible protein 14
    Short name:
    Protein GCD14
    tRNA(m1A58)-methyltransferase subunit TRM61
    Short name:
    tRNA(m1A58)MTase subunit TRM61
    Gene namesi
    Name:GCD14
    Synonyms:TRM61
    Ordered Locus Names:YJL125C
    ORF Names:J0710
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL125c.
    SGDiS000003661. GCD14.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: SGD
    2. tRNA (m1A) methyltransferase complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 383383tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61PRO_0000087437Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei302 – 3021Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP46959.
    PaxDbiP46959.
    PeptideAtlasiP46959.

    Expressioni

    Gene expression databases

    GenevestigatoriP46959.

    Interactioni

    Subunit structurei

    tRNA (adenine-N(1)-)-methyltransferase is a heterodimer of TRM6/GCD10 and TRM61/GCD14.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GCD10P418143EBI-7416,EBI-8995

    Protein-protein interaction databases

    BioGridi33631. 73 interactions.
    DIPiDIP-5421N.
    IntActiP46959. 17 interactions.
    MINTiMINT-540985.
    STRINGi4932.YJL125C.

    Structurei

    3D structure databases

    ProteinModelPortaliP46959.
    SMRiP46959. Positions 11-272.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM61 family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2519.
    GeneTreeiENSGT00510000048063.
    HOGENOMiHOG000173282.
    KOiK07442.
    OMAiVEIQGRQ.
    OrthoDBiEOG7B8SCW.

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR014816. tRNA_MeTrfase_Gcd14.
    [Graphical view]
    PfamiPF08704. GCD14. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017269. GCD14. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51620. SAM_TRM61. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46959-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTNCFSGYK DLIKEGDLTL IWVSRDNIKP VRMHSEEVFN TRYGSFPHKD    50
    IIGKPYGSQI AIRTKGSNKF AFVHVLQPTP ELWTLSLPHR TQIVYTPDSS 100
    YIMQRLNCSP HSRVIEAGTG SGSFSHAFAR SVGHLFSFEF HHIRYEQALE 150
    EFKEHGLIDD NVTITHRDVC QGGFLIKKGD TTSYEFGNNE TAASLNANVV 200
    FLDLPAPWDA IPHLDSVISV DEKVGLCCFS PCIEQVDKTL DVLEKYGWTD 250
    VEMVEIQGRQ YESRRQMVRS LNDALERLRD IKRHKLQGVE RRKRMFNNTI 300
    DSNDEKVGKR NEDGVPLTEK AKFNPFGKGS RIKEGDSNYK WKEVTKMEAE 350
    IKSHTSYLTF AFKVVNRSRD DEKVNEILRS TEK 383
    Length:383
    Mass (Da):43,920
    Last modified:November 1, 1995 - v1
    Checksum:iA8402DF77A628FD3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z54149 Genomic DNA. Translation: CAA90863.1.
    Z49400 Genomic DNA. Translation: CAA89420.1.
    BK006943 Genomic DNA. Translation: DAA08677.1.
    PIRiS56906.
    RefSeqiNP_012410.1. NM_001181558.1.

    Genome annotation databases

    EnsemblFungiiYJL125C; YJL125C; YJL125C.
    GeneIDi853317.
    KEGGisce:YJL125C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z54149 Genomic DNA. Translation: CAA90863.1 .
    Z49400 Genomic DNA. Translation: CAA89420.1 .
    BK006943 Genomic DNA. Translation: DAA08677.1 .
    PIRi S56906.
    RefSeqi NP_012410.1. NM_001181558.1.

    3D structure databases

    ProteinModelPortali P46959.
    SMRi P46959. Positions 11-272.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33631. 73 interactions.
    DIPi DIP-5421N.
    IntActi P46959. 17 interactions.
    MINTi MINT-540985.
    STRINGi 4932.YJL125C.

    Proteomic databases

    MaxQBi P46959.
    PaxDbi P46959.
    PeptideAtlasi P46959.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL125C ; YJL125C ; YJL125C .
    GeneIDi 853317.
    KEGGi sce:YJL125C.

    Organism-specific databases

    CYGDi YJL125c.
    SGDi S000003661. GCD14.

    Phylogenomic databases

    eggNOGi COG2519.
    GeneTreei ENSGT00510000048063.
    HOGENOMi HOG000173282.
    KOi K07442.
    OMAi VEIQGRQ.
    OrthoDBi EOG7B8SCW.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-16691.
    YEAST:G3O-31576-MONOMER.

    Miscellaneous databases

    NextBioi 973663.
    PROi P46959.

    Gene expression databases

    Genevestigatori P46959.

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR014816. tRNA_MeTrfase_Gcd14.
    [Graphical view ]
    Pfami PF08704. GCD14. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017269. GCD14. 1 hit.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS51620. SAM_TRM61. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cuesta R.
      Thesis (1996), University of Salamanca, Spain
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / GRF88.
    2. "Sequencing analysis of a 40.2 kb fragment of yeast chromosome X reveals 19 open reading frames including URA2 (5' end), TRK1, PBS2, SPT10, GCD14, RPE1, PHO86, NCA3, ASF1, CCT7, GZF3, two tRNA genes, three remnant delta elements and a Ty4 transposon."
      Cziepluch C., Kordes E., Pujol A., Jauniaux J.-C.
      Yeast 12:1471-1474(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA."
      Anderson J., Phan L., Cuesta R., Carlson B.A., Pak M., Asano K., Bjoerk G.R., Tamame M., Hinnebusch A.G.
      Genes Dev. 12:3650-3662(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A TRNA METHYLTRANSFERASE, SUBUNIT, SUBCELLULAR LOCATION.
    6. "Identification of GCD14 and GCD15, novel genes required for translational repression of GCN4 mRNA in Saccharomyces cerevisiae."
      Cuesta R., Hinnebusch A.G., Tamame M.
      Genetics 148:1007-1020(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN TRANSLATIONAL REPRESSION.
    7. "The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae."
      Anderson J., Phan L., Hinnebusch A.G.
      Proc. Natl. Acad. Sci. U.S.A. 97:5173-5178(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A TRNA METHYLTRANSFERASE.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTRM61_YEAST
    AccessioniPrimary (citable) accession number: P46959
    Secondary accession number(s): D6VW61
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7220 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3