tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P46959 (TRM61_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61
EC=2.1.1.220

Alternative name(s):
General control non-derepressible protein 14
Short name=Protein GCD14
tRNA(m1A58)-methyltransferase subunit TRM61
Short name=tRNA(m1A58)MTase subunit TRM61

Gene names

Name:	GCD14
Synonyms:	TRM61
Ordered Locus Names:	YJL125C
ORF Names:	J0710

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. GCD14 is also required for repression of GCN4 mRNA translation by the upstream open reading frames (uORFs) under conditions of amino acid sufficiency. Ref.5 Ref.6 Ref.7
Catalytic activity	S-adenosyl-L-methionine + adenine₅₈ in tRNA = S-adenosyl-L-homocysteine + N(1)-methyladenine₅₈ in tRNA.
Subunit structure	tRNA (adenine-N(1)-)-methyltransferase is a heterodimer of TRM6/GCD10 and TRM61/GCD14. Ref.5
Subcellular location	Nucleus Ref.5.
Miscellaneous	Present with 7220 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the methyltransferase superfamily. TRM61 family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Repressor Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA methylation Inferred from direct assay Ref.7. Source: SGD
Cellular_component	nucleus Inferred from direct assay Ref.5. Source: SGD tRNA (m1A) methyltransferase complex Inferred from direct assay Ref.7. Source: SGD
Molecular_function	tRNA (adenine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GCD10	P41814	3	EBI-7416,EBI-8995

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 383

383

tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61

PRO_0000087437

Sites

Binding site

121

S-adenosyl-L-methionine By similarity

Binding site

139

S-adenosyl-L-methionine By similarity

Binding site

168

S-adenosyl-L-methionine By similarity

Binding site

203

S-adenosyl-L-methionine By similarity

Amino acid modifications

Modified residue

302

Phosphoserine Ref.9

Sequences

Sequence

Length

Mass (Da)

Tools

P46959 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: A8402DF77A628FD3
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FASTA

383

43,920

        10         20         30         40         50         60 
MSTNCFSGYK DLIKEGDLTL IWVSRDNIKP VRMHSEEVFN TRYGSFPHKD IIGKPYGSQI 

        70         80         90        100        110        120 
AIRTKGSNKF AFVHVLQPTP ELWTLSLPHR TQIVYTPDSS YIMQRLNCSP HSRVIEAGTG 

       130        140        150        160        170        180 
SGSFSHAFAR SVGHLFSFEF HHIRYEQALE EFKEHGLIDD NVTITHRDVC QGGFLIKKGD 

       190        200        210        220        230        240 
TTSYEFGNNE TAASLNANVV FLDLPAPWDA IPHLDSVISV DEKVGLCCFS PCIEQVDKTL 

       250        260        270        280        290        300 
DVLEKYGWTD VEMVEIQGRQ YESRRQMVRS LNDALERLRD IKRHKLQGVE RRKRMFNNTI 

       310        320        330        340        350        360 
DSNDEKVGKR NEDGVPLTEK AKFNPFGKGS RIKEGDSNYK WKEVTKMEAE IKSHTSYLTF 

       370        380 
AFKVVNRSRD DEKVNEILRS TEK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Cuesta R. Thesis (1996), University of Salamanca, Spain Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[2]	"Sequencing analysis of a 40.2 kb fragment of yeast chromosome X reveals 19 open reading frames including URA2 (5' end), TRK1, PBS2, SPT10, GCD14, RPE1, PHO86, NCA3, ASF1, CCT7, GZF3, two tRNA genes, three remnant delta elements and a Ty4 transposon." Cziepluch C., Kordes E., Pujol A., Jauniaux J.-C. Yeast 12:1471-1474(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA." Anderson J., Phan L., Cuesta R., Carlson B.A., Pak M., Asano K., Bjoerk G.R., Tamame M., Hinnebusch A.G. Genes Dev. 12:3650-3662(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A TRNA METHYLTRANSFERASE, SUBUNIT, SUBCELLULAR LOCATION.
[6]	"Identification of GCD14 and GCD15, novel genes required for translational repression of GCN4 mRNA in Saccharomyces cerevisiae." Cuesta R., Hinnebusch A.G., Tamame M. Genetics 148:1007-1020(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN TRANSLATIONAL REPRESSION.
[7]	"The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae." Anderson J., Phan L., Hinnebusch A.G. Proc. Natl. Acad. Sci. U.S.A. 97:5173-5178(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A TRNA METHYLTRANSFERASE.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z54149 Genomic DNA. Translation: CAA90863.1. Z49400 Genomic DNA. Translation: CAA89420.1. BK006943 Genomic DNA. Translation: DAA08677.1.
PIR	S56906.
RefSeq	NP_012410.1. NM_001181558.1.
3D structure databases
ProteinModelPortal	P46959.
SMR	P46959. Positions 11-272.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5421N.
IntAct	P46959. 17 interactions.
MINT	MINT-540985.
STRING	4932.YJL125C.
Proteomic databases
PaxDb	P46959.
PeptideAtlas	P46959.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL125C; YJL125C; YJL125C.
GeneID	853317.
KEGG	sce:YJL125C.
Organism-specific databases
CYGD	YJL125c.
SGD	S000003661. GCD14.
Phylogenomic databases
eggNOG	COG2519.
GeneTree	ENSGT00510000048063.
HOGENOM	HOG000173282.
KO	K07442.
OMA	VEIQGRQ.
OrthoDB	EOG4ZCXDD.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16691.
Gene expression databases
Genevestigator	P46959.
GermOnline	YJL125C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR014816. tRNA_MeTrfase_GCD14. IPR025809. tRNA_MeTrfase_GCD14_euk. [Graphical view]
Pfam	PF08704. GCD14. 1 hit. [Graphical view]
PIRSF	PIRSF017269. GCD14. 1 hit.
ProtoNet	Search...
Other
NextBio	973663.

Entry information

Entry name

TRM61_YEAST

Accession

Primary (citable) accession number: P46959
Secondary accession number(s): D6VW61

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents