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Protein

DNA polymerase delta small subunit

Gene

POL31

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA polymerase delta (DNA polymerase III) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  • DNA binding Source: InterPro
  • DNA-directed DNA polymerase activity Source: SGD

GO - Biological processi

  • base-excision repair Source: SGD
  • DNA replication, removal of RNA primer Source: SGD
  • lagging strand elongation Source: SGD
  • leading strand elongation Source: SGD
  • mismatch repair Source: SGD
  • nucleic acid phosphodiester bond hydrolysis Source: GOC
  • nucleotide-excision repair Source: SGD
  • postreplication repair Source: SGD
  • RNA-dependent DNA biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-31652-MONOMER.
BRENDAi2.7.7.7. 984.
ReactomeiR-SCE-69166. Removal of the Flap Intermediate.
R-SCE-69183. Processive synthesis on the lagging strand.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase delta small subunit (EC:2.7.7.7)
Alternative name(s):
Hydroxyurea-sensitive protein 2
Gene namesi
Name:POL31
Synonyms:HUS2, HYS2, SDP5
Ordered Locus Names:YJR006W
ORF Names:J1427, YJR83.7
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR006W.
SGDiS000003766. POL31.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • delta DNA polymerase complex Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 487487DNA polymerase delta small subunitPRO_0000096174Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources
Modified residuei20 – 201PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP46957.

PTM databases

iPTMnetiP46957.

Interactioni

Subunit structurei

DNA polymerase delta is a heterotrimer of POL3, POL32 and HYS2.

Binary interactionsi

WithEntry#Exp.IntActNotes
POL3P154363EBI-6080,EBI-6134

Protein-protein interaction databases

BioGridi33762. 43 interactions.
DIPiDIP-2525N.
IntActiP46957. 5 interactions.
MINTiMINT-477144.

Structurei

3D structure databases

ProteinModelPortaliP46957.
SMRiP46957. Positions 51-483.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000006780.
HOGENOMiHOG000189057.
InParanoidiP46957.
KOiK02328.
OMAiWQNIAPT.
OrthoDBiEOG092C3F88.

Family and domain databases

InterProiIPR007185. DNA_pol_alpha/epsilon_bsu.
IPR024826. DNA_pol_delta/II_ssu.
[Graphical view]
PANTHERiPTHR10416. PTHR10416. 2 hits.
PfamiPF04042. DNA_pol_E_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46957-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDALLTKFNE DRSLQDENLS QPRTRVRIVD DNLYNKSNPF QLCYKKRDYG
60 70 80 90 100
SQYYHIYQYR LKTFRERVLK ECDKRWDAGF TLNGQLVLKK DKVLDIQGNQ
110 120 130 140 150
PCWCVGSIYC EMKYKPNVLD EVINDTYGAP DLTKSYTDKE GGSDEIMLED
160 170 180 190 200
ESGRVLLVGD FIRSTPFITG VVVGILGMEA EAGTFQVLDI CYPTPLPQNP
210 220 230 240 250
FPAPIATCPT RGKIALVSGL NLNNTSPDRL LRLEILREFL MGRINNKIDD
260 270 280 290 300
ISLIGRLLIC GNSVDFDIKS VNKDELMISL TEFSKFLHNI LPSISVDIMP
310 320 330 340 350
GTNDPSDKSL PQQPFHKSLF DKSLESYFNG SNKEILNLVT NPYEFSYNGV
360 370 380 390 400
DVLAVSGKNI NDICKYVIPS NDNGESENKV EEGESNDFKD DIEHRLDLME
410 420 430 440 450
CTMKWQNIAP TAPDTLWCYP YTDKDPFVLD KWPHVYIVAN QPYFGTRVVE
460 470 480
IGGKNIKIIS VPEFSSTGMI ILLDLETLEA ETVKIDI
Length:487
Mass (Da):55,296
Last modified:November 1, 1995 - v1
Checksum:iF9E200BFE97A2C07
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti156 – 1561L → H in BAA08859 (PubMed:7567461).Curated
Sequence conflicti465 – 4651S → N in BAA08859 (PubMed:7567461).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50324 Genomic DNA. Translation: BAA08859.1.
X87611 Genomic DNA. Translation: CAA60928.1.
Z49506 Genomic DNA. Translation: CAA89528.1.
BK006943 Genomic DNA. Translation: DAA08797.1.
PIRiS55194.
RefSeqiNP_012539.1. NM_001181663.1.

Genome annotation databases

EnsemblFungiiYJR006W; YJR006W; YJR006W.
GeneIDi853462.
KEGGisce:YJR006W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50324 Genomic DNA. Translation: BAA08859.1.
X87611 Genomic DNA. Translation: CAA60928.1.
Z49506 Genomic DNA. Translation: CAA89528.1.
BK006943 Genomic DNA. Translation: DAA08797.1.
PIRiS55194.
RefSeqiNP_012539.1. NM_001181663.1.

3D structure databases

ProteinModelPortaliP46957.
SMRiP46957. Positions 51-483.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33762. 43 interactions.
DIPiDIP-2525N.
IntActiP46957. 5 interactions.
MINTiMINT-477144.

PTM databases

iPTMnetiP46957.

Proteomic databases

MaxQBiP46957.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR006W; YJR006W; YJR006W.
GeneIDi853462.
KEGGisce:YJR006W.

Organism-specific databases

EuPathDBiFungiDB:YJR006W.
SGDiS000003766. POL31.

Phylogenomic databases

GeneTreeiENSGT00390000006780.
HOGENOMiHOG000189057.
InParanoidiP46957.
KOiK02328.
OMAiWQNIAPT.
OrthoDBiEOG092C3F88.

Enzyme and pathway databases

BioCyciYEAST:G3O-31652-MONOMER.
BRENDAi2.7.7.7. 984.
ReactomeiR-SCE-69166. Removal of the Flap Intermediate.
R-SCE-69183. Processive synthesis on the lagging strand.

Miscellaneous databases

PROiP46957.

Family and domain databases

InterProiIPR007185. DNA_pol_alpha/epsilon_bsu.
IPR024826. DNA_pol_delta/II_ssu.
[Graphical view]
PANTHERiPTHR10416. PTHR10416. 2 hits.
PfamiPF04042. DNA_pol_E_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPOD2_YEAST
AccessioniPrimary (citable) accession number: P46957
Secondary accession number(s): D6VWI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 626 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.