ID   PHO86_YEAST             Reviewed;         311 AA.
AC   P46956; D6VW67; E9P8T7;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   27-MAR-2024, entry version 160.
DE   RecName: Full=Inorganic phosphate transporter PHO86;
GN   Name=PHO86; OrderedLocusNames=YJL117W; ORFNames=J0744;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=S288c / GRF88;
RX   PubMed=8598055; DOI=10.1007/s002940050055;
RA   Bun-Ya M., Shikata K., Nakade S., Yompakdee C., Harashima S., Oshima Y.;
RT   "Two new genes, PHO86 and PHO87, involved in inorganic phosphate uptake in
RT   Saccharomyces cerevisiae.";
RL   Curr. Genet. 29:344-351(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   PubMed=8948101;
RX   DOI=10.1002/(sici)1097-0061(199611)12:14<1471::aid-yea30>3.0.co;2-4;
RA   Cziepluch C., Kordes E., Pujol A., Jauniaux J.-C.;
RT   "Sequencing analysis of a 40.2 kb fragment of yeast chromosome X reveals 19
RT   open reading frames including URA2 (5' end), TRK1, PBS2, SPT10, GCD14,
RT   RPE1, PHO86, NCA3, ASF1, CCT7, GZF3, two tRNA genes, three remnant delta
RT   elements and a Ty4 transposon.";
RL   Yeast 12:1471-1474(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA   Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA   Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA   Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA   Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA   Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA   Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA   Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA   Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA   To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA   von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL   EMBO J. 15:2031-2049(1996).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [6]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 208353 / W303-1A;
RX   PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA   Kim H., Melen K., Oesterberg M., von Heijne G.;
RT   "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC   -!- FUNCTION: Involved in the uptake of inorganic phosphate.
CC   -!- INTERACTION:
CC       P46956; P40207: ERG29; NbExp=3; IntAct=EBI-13337, EBI-27272;
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; D63817; BAA09885.1; -; Genomic_DNA.
DR   EMBL; Z49392; CAA89412.1; -; Genomic_DNA.
DR   EMBL; AY557877; AAS56203.1; -; Genomic_DNA.
DR   EMBL; BK006943; DAA08683.1; -; Genomic_DNA.
DR   PIR; S56898; S56898.
DR   RefSeq; NP_012418.1; NM_001181550.1.
DR   AlphaFoldDB; P46956; -.
DR   BioGRID; 33637; 291.
DR   DIP; DIP-2769N; -.
DR   IntAct; P46956; 46.
DR   MINT; P46956; -.
DR   STRING; 4932.YJL117W; -.
DR   MaxQB; P46956; -.
DR   PaxDb; 4932-YJL117W; -.
DR   PeptideAtlas; P46956; -.
DR   EnsemblFungi; YJL117W_mRNA; YJL117W; YJL117W.
DR   GeneID; 853325; -.
DR   KEGG; sce:YJL117W; -.
DR   AGR; SGD:S000003653; -.
DR   SGD; S000003653; PHO86.
DR   VEuPathDB; FungiDB:YJL117W; -.
DR   eggNOG; ENOG502QSU5; Eukaryota.
DR   HOGENOM; CLU_076919_0_0_1; -.
DR   InParanoid; P46956; -.
DR   OMA; ELWGVQF; -.
DR   OrthoDB; 2048847at2759; -.
DR   BioCyc; YEAST:G3O-31571-MONOMER; -.
DR   BioGRID-ORCS; 853325; 1 hit in 10 CRISPR screens.
DR   PRO; PR:P46956; -.
DR   Proteomes; UP000002311; Chromosome X.
DR   RNAct; P46956; Protein.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:SGD.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0051082; F:unfolded protein binding; IMP:SGD.
DR   GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IMP:SGD.
DR   GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0006457; P:protein folding; IMP:SGD.
DR   GO; GO:0010966; P:regulation of phosphate transport; IMP:SGD.
DR   InterPro; IPR024297; Pho86.
DR   Pfam; PF11124; Pho86; 1.
PE   1: Evidence at protein level;
KW   Membrane; Phosphate transport; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..311
FT                   /note="Inorganic phosphate transporter PHO86"
FT                   /id="PRO_0000058404"
FT   TOPO_DOM        1..70
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        71..91
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        92..117
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        118..138
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        139..311
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          1..31
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..16
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        8
FT                   /note="K -> R (in Ref. 5; AAS56203)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   311 AA;  34882 MW;  1CB19C9184BF1C48 CRC64;
     MAVQQRKKKE GRKSDKNAPS VPQVDASLDK PLDIDAPPTI YSVNLKPEYG TAALNLSADF
     IRQEQALANK YLFFHPVILV VLTIGLLIYL TPRIVFPIRN TGSVAGWFYQ LARINKKVVL
     SGLVFTAIGA SFLFTLLSRV SDSYFKSKIN QLVGSKGEKV FGINLNDLVA RHETKDPVVN
     NTHIIVYRET PIALISLAPN MTLSTDENLV MSVTTVGCRR VYVKSGIIED LIDWAMLHSK
     NIRSSGKYGE TMKLLIDVYS FDSTLKEILK KKGFTYIQSI RVSENRLLGG LFGVKKELWG
     LQFHFKAEHK D
//