P46956 (PHO86_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic phosphate transporter PHO86

Gene names

Name:	PHO86
Ordered Locus Names:	YJL117W
ORF Names:	J0744

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the uptake of inorganic phosphate.
Subcellular location	Membrane; Multi-pass membrane protein Potential.

Ontologies

Keywords
Biological process	Phosphate transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from mutant phenotype PubMed 10655492. Source: SGD phosphate ion transport Inferred from electronic annotation. Source: UniProtKB-KW protein folding Inferred from mutant phenotype PubMed 15623581. Source: SGD regulation of phosphate transport Inferred from mutant phenotype PubMed 10655492. Source: SGD
Cellular_component	endoplasmic reticulum Inferred from direct assay PubMed 10655492. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	unfolded protein binding Inferred from mutant phenotype PubMed 15623581. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YMR134W	P40207	3	EBI-13337,EBI-27272

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Inorganic phosphate transporter PHO86		PRO_0000058404
Regions
	Topological domain	1 – 70	70	Cytoplasmic Potential
	Transmembrane	71 – 91	21	Helical; Potential
	Topological domain	92 – 117	26	Extracellular Potential
	Transmembrane	118 – 138	21	Helical; Potential
	Topological domain	139 – 311	173	Cytoplasmic Potential
Experimental info
	Sequence conflict	8	1	K → R in AAS56203. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P46956 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 1CB19C9184BF1C48	FASTA	311	34,882
	10 20 30 40 50 60 MAVQQRKKKE GRKSDKNAPS VPQVDASLDK PLDIDAPPTI YSVNLKPEYG TAALNLSADF 70 80 90 100 110 120 IRQEQALANK YLFFHPVILV VLTIGLLIYL TPRIVFPIRN TGSVAGWFYQ LARINKKVVL 130 140 150 160 170 180 SGLVFTAIGA SFLFTLLSRV SDSYFKSKIN QLVGSKGEKV FGINLNDLVA RHETKDPVVN 190 200 210 220 230 240 NTHIIVYRET PIALISLAPN MTLSTDENLV MSVTTVGCRR VYVKSGIIED LIDWAMLHSK 250 260 270 280 290 300 NIRSSGKYGE TMKLLIDVYS FDSTLKEILK KKGFTYIQSI RVSENRLLGG LFGVKKELWG 310 LQFHFKAEHK D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two new genes, PHO86 and PHO87, involved in inorganic phosphate uptake in Saccharomyces cerevisiae." Bun-Ya M., Shikata K., Nakade S., Yompakdee C., Harashima S., Oshima Y. Curr. Genet. 29:344-351(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[2]	"Sequencing analysis of a 40.2 kb fragment of yeast chromosome X reveals 19 open reading frames including URA2 (5' end), TRK1, PBS2, SPT10, GCD14, RPE1, PHO86, NCA3, ASF1, CCT7, GZF3, two tRNA genes, three remnant delta elements and a Ty4 transposon." Cziepluch C., Kordes E., Pujol A., Jauniaux J.-C. Yeast 12:1471-1474(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D63817 Genomic DNA. Translation: BAA09885.1. Z49392 Genomic DNA. Translation: CAA89412.1. AY557877 Genomic DNA. Translation: AAS56203.1. BK006943 Genomic DNA. Translation: DAA08683.1.
PIR	S56898.
RefSeq	NP_012418.1. NM_001181550.1.
3D structure databases
ProteinModelPortal	P46956.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2769N.
IntAct	P46956. 35 interactions.
MINT	MINT-521701.
STRING	4932.YJL117W.
Proteomic databases
PaxDb	P46956.
PeptideAtlas	P46956.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL117W; YJL117W; YJL117W.
GeneID	853325.
KEGG	sce:YJL117W.
Organism-specific databases
CYGD	YJL117w.
SGD	S000003653. PHO86.
Phylogenomic databases
eggNOG	NOG47096.
HOGENOM	HOG000000832.
OMA	YRETPIA.
OrthoDB	EOG4J40SC.
Gene expression databases
Genevestigator	P46956.
GermOnline	YJL117W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR024297. Pho86. [Graphical view]
Pfam	PF11124. Pho86. 1 hit. [Graphical view]
ProDom	PD130611. PD130611. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other
NextBio	973681.

Entry information

Entry name

PHO86_YEAST

Accession

Primary (citable) accession number: P46956
Secondary accession number(s): D6VW67, E9P8T7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program