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Protein

3-hydroxyanthranilate 3,4-dioxygenase

Gene

Haao

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.UniRule annotation1 Publication

Catalytic activityi

3-hydroxyanthranilate + O2 = 2-amino-3-carboxymuconate semialdehyde.UniRule annotation1 Publication

Cofactori

Fe2+UniRule annotation1 Publication

Kineticsi

  1. KM=3.6 µM for 3-hydroxyanthranilate1 Publication
  1. Vmax=73.7 pmol/h/mg enzyme1 Publication

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes quinolinate from L-kynurenine.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Kynurenine 3-monooxygenase (Kmo)
  2. Kynureninase (Kynu)
  3. 3-hydroxyanthranilate 3,4-dioxygenase (Haao), 3-hydroxyanthranilate 3,4-dioxygenase (Haao)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from L-kynurenine, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43DioxygenUniRule annotation1
Metal bindingi47Iron; catalyticUniRule annotation1
Metal bindingi53Iron; catalyticUniRule annotation1
Binding sitei53SubstrateUniRule annotation1
Metal bindingi91Iron; catalyticUniRule annotation1
Binding sitei95SubstrateUniRule annotation1
Binding sitei105SubstrateUniRule annotation1

GO - Molecular functioni

  • 3-hydroxyanthranilate 3,4-dioxygenase activity Source: RGD
  • ferrous iron binding Source: RGD
  • iron ion binding Source: RGD
  • oxygen binding Source: RGD

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processPyridine nucleotide biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00253; UER00330

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxyanthranilate 3,4-dioxygenaseUniRule annotation (EC:1.13.11.6UniRule annotation1 Publication)
Alternative name(s):
3-hydroxyanthranilate oxygenaseUniRule annotation
Short name:
3-HAOUniRule annotation
3-hydroxyanthranilic acid dioxygenaseUniRule annotation
Short name:
HADUniRule annotation
Gene namesi
Name:Haao
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi71071 Haao

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3108658

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000643741 – 2863-hydroxyanthranilate 3,4-dioxygenaseAdd BLAST286

Proteomic databases

PaxDbiP46953
PRIDEiP46953

PTM databases

iPTMnetiP46953
PhosphoSitePlusiP46953

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043835

Chemistry databases

BindingDBiP46953

Structurei

3D structure databases

ProteinModelPortaliP46953
SMRiP46953
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 160Domain A (catalytic)UniRule annotationAdd BLAST160
Regioni161 – 177LinkerUniRule annotationAdd BLAST17
Regioni178 – 286Domain BUniRule annotationAdd BLAST109

Sequence similaritiesi

Belongs to the 3-HAO family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3995 Eukaryota
ENOG4111GH8 LUCA
HOGENOMiHOG000218448
HOVERGENiHBG000018
InParanoidiP46953
KOiK00452
PhylomeDBiP46953
TreeFamiTF300246

Family and domain databases

Gene3Di2.60.120.101 hit
HAMAPiMF_00825 3_HAO, 1 hit
InterProiView protein in InterPro
IPR010329 3hydroanth_dOase
IPR016700 3hydroanth_dOase_met
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PANTHERiPTHR15497 PTHR15497, 1 hit
PfamiView protein in Pfam
PF06052 3-HAO, 1 hit
PIRSFiPIRSF017681 3hydroanth_dOase_animal, 1 hit
SUPFAMiSSF51182 SSF51182, 2 hits
TIGRFAMsiTIGR03037 anthran_nbaC, 1 hit

Sequencei

Sequence statusi: Complete.

P46953-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERCVRVKSW VEENRASFQP PVCNKLMHRE QLKIMFVGGP NTRKDYHIEE
60 70 80 90 100
GEEVFYQLEG DMVLRVLEQG EHRDVVIRQG EIFLLPARVP HSPQRFANTM
110 120 130 140 150
GLVIERRRME TELDGLRYYV GDTEDVLFEK WFHCKDLGTQ LAPIIQEFFH
160 170 180 190 200
SEQYRTGKPN PDQLLKEPPF PLSTRSVMEP MSLKAWLESH SRELQAGTSL
210 220 230 240 250
SLFGDSYETQ VIAHGQGSSK GPRQDVDVWL WQLEGSSKVT MGGQCVALAP
260 270 280
DDSLLVPAGF SYMWERAQGS VALSVTQDPA CKKPLG
Length:286
Mass (Da):32,582
Last modified:December 15, 1998 - v2
Checksum:iB4F535AD8949DAB7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29R → Q in AAH85739 (PubMed:15489334).Curated1
Sequence conflicti44K → S AA sequence (PubMed:7514594).Curated1
Sequence conflicti199S → F AA sequence (PubMed:7514594).Curated1
Sequence conflicti204G → C AA sequence (PubMed:7514594).Curated1
Sequence conflicti214H → Y AA sequence (PubMed:7514594).Curated1
Sequence conflicti229W → P AA sequence (PubMed:7514594).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D44494 mRNA Translation: BAA07937.1
BC085739 mRNA Translation: AAH85739.1
D28339 mRNA Translation: BAA21019.1
RefSeqiNP_064461.1, NM_020076.2
UniGeneiRn.48675

Genome annotation databases

GeneIDi56823
KEGGirno:56823
UCSCiRGD:71071 rat

Similar proteinsi

Entry informationi

Entry namei3HAO_RAT
AccessioniPrimary (citable) accession number: P46953
Secondary accession number(s): P70474, Q5RKK0, Q64556
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 15, 1998
Last modified: March 28, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome