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P46953

- 3HAO_RAT

UniProt

P46953 - 3HAO_RAT

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Protein
3-hydroxyanthranilate 3,4-dioxygenase
Gene
Haao
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate By similarity.UniRule annotation

Catalytic activityi

3-hydroxyanthranilate + O2 = 2-amino-3-carboxymuconate semialdehyde.UniRule annotation

Cofactori

Fe2+ ion By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431Dioxygen By similarity
Metal bindingi47 – 471Iron; catalytic By similarity
Metal bindingi53 – 531Iron; catalytic By similarity
Binding sitei53 – 531Substrate By similarity
Metal bindingi91 – 911Iron; catalytic By similarity
Binding sitei95 – 951Substrate By similarity
Binding sitei105 – 1051Substrate By similarity

GO - Molecular functioni

  1. 3-hydroxyanthranilate 3,4-dioxygenase activity Source: RGD
  2. ferrous iron binding Source: UniProtKB-HAMAP
  3. iron ion binding Source: RGD
  4. oxygen binding Source: RGD

GO - Biological processi

  1. 'de novo' NAD biosynthetic process from tryptophan Source: UniProtKB-HAMAP
  2. anthranilate metabolic process Source: UniProtKB-HAMAP
  3. neuron cellular homeostasis Source: Ensembl
  4. quinolinate biosynthetic process Source: UniProtKB-HAMAP
  5. quinolinate metabolic process Source: RGD
  6. response to cadmium ion Source: Ensembl
  7. response to zinc ion Source: Ensembl
  8. tryptophan catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_222206. Tryptophan catabolism.
UniPathwayiUPA00253; UER00330.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxyanthranilate 3,4-dioxygenase (EC:1.13.11.6)
Alternative name(s):
3-hydroxyanthranilate oxygenase
Short name:
3-HAO
3-hydroxyanthranilic acid dioxygenase
Short name:
HAD
Gene namesi
Name:Haao
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 6

Organism-specific databases

RGDi71071. Haao.

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. cytosol Source: Ensembl
  3. mitochondrial membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2862863-hydroxyanthranilate 3,4-dioxygenaseUniRule annotation
PRO_0000064374Add
BLAST

Proteomic databases

PaxDbiP46953.
PRIDEiP46953.

PTM databases

PhosphoSiteiP46953.

Expressioni

Gene expression databases

GenevestigatoriP46953.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

MINTiMINT-4569623.

Structurei

3D structure databases

ProteinModelPortaliP46953.
SMRiP46953. Positions 2-286.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 160160Domain A (catalytic) By similarity
Add
BLAST
Regioni161 – 17717Linker By similarity
Add
BLAST
Regioni178 – 286109Domain B By similarity
Add
BLAST

Sequence similaritiesi

Belongs to the 3-HAO family.

Phylogenomic databases

eggNOGiNOG77058.
GeneTreeiENSGT00390000013008.
HOGENOMiHOG000218448.
HOVERGENiHBG000018.
KOiK00452.
OrthoDBiEOG7FFMS4.
PhylomeDBiP46953.
TreeFamiTF300246.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00825. 3_HAO.
InterProiIPR010329. 3hydroanth_dOase.
IPR016700. 3hydroanth_dOase_met.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR15497. PTHR15497. 1 hit.
PfamiPF06052. 3-HAO. 1 hit.
[Graphical view]
PIRSFiPIRSF017681. 3hydroanth_dOase_animal. 1 hit.
SUPFAMiSSF51182. SSF51182. 2 hits.
TIGRFAMsiTIGR03037. anthran_nbaC. 1 hit.

Sequencei

Sequence statusi: Complete.

P46953-1 [UniParc]FASTAAdd to Basket

« Hide

MERCVRVKSW VEENRASFQP PVCNKLMHRE QLKIMFVGGP NTRKDYHIEE    50
GEEVFYQLEG DMVLRVLEQG EHRDVVIRQG EIFLLPARVP HSPQRFANTM 100
GLVIERRRME TELDGLRYYV GDTEDVLFEK WFHCKDLGTQ LAPIIQEFFH 150
SEQYRTGKPN PDQLLKEPPF PLSTRSVMEP MSLKAWLESH SRELQAGTSL 200
SLFGDSYETQ VIAHGQGSSK GPRQDVDVWL WQLEGSSKVT MGGQCVALAP 250
DDSLLVPAGF SYMWERAQGS VALSVTQDPA CKKPLG 286
Length:286
Mass (Da):32,582
Last modified:December 15, 1998 - v2
Checksum:iB4F535AD8949DAB7
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291R → Q in AAH85739. 1 Publication
Sequence conflicti44 – 441K → S AA sequence 1 Publication
Sequence conflicti199 – 1991S → F AA sequence 1 Publication
Sequence conflicti204 – 2041G → C AA sequence 1 Publication
Sequence conflicti214 – 2141H → Y AA sequence 1 Publication
Sequence conflicti229 – 2291W → P AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D44494 mRNA. Translation: BAA07937.1.
BC085739 mRNA. Translation: AAH85739.1.
D28339 mRNA. Translation: BAA21019.1.
RefSeqiNP_064461.1. NM_020076.2.
UniGeneiRn.48675.

Genome annotation databases

EnsembliENSRNOT00000045996; ENSRNOP00000043835; ENSRNOG00000031263.
GeneIDi56823.
KEGGirno:56823.
UCSCiRGD:71071. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D44494 mRNA. Translation: BAA07937.1 .
BC085739 mRNA. Translation: AAH85739.1 .
D28339 mRNA. Translation: BAA21019.1 .
RefSeqi NP_064461.1. NM_020076.2.
UniGenei Rn.48675.

3D structure databases

ProteinModelPortali P46953.
SMRi P46953. Positions 2-286.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4569623.

PTM databases

PhosphoSitei P46953.

Proteomic databases

PaxDbi P46953.
PRIDEi P46953.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000045996 ; ENSRNOP00000043835 ; ENSRNOG00000031263 .
GeneIDi 56823.
KEGGi rno:56823.
UCSCi RGD:71071. rat.

Organism-specific databases

CTDi 23498.
RGDi 71071. Haao.

Phylogenomic databases

eggNOGi NOG77058.
GeneTreei ENSGT00390000013008.
HOGENOMi HOG000218448.
HOVERGENi HBG000018.
KOi K00452.
OrthoDBi EOG7FFMS4.
PhylomeDBi P46953.
TreeFami TF300246.

Enzyme and pathway databases

UniPathwayi UPA00253 ; UER00330 .
Reactomei REACT_222206. Tryptophan catabolism.

Miscellaneous databases

NextBioi 611253.
PROi P46953.

Gene expression databases

Genevestigatori P46953.

Family and domain databases

Gene3Di 2.60.120.10. 2 hits.
HAMAPi MF_00825. 3_HAO.
InterProi IPR010329. 3hydroanth_dOase.
IPR016700. 3hydroanth_dOase_met.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view ]
PANTHERi PTHR15497. PTHR15497. 1 hit.
Pfami PF06052. 3-HAO. 1 hit.
[Graphical view ]
PIRSFi PIRSF017681. 3hydroanth_dOase_animal. 1 hit.
SUPFAMi SSF51182. SSF51182. 2 hits.
TIGRFAMsi TIGR03037. anthran_nbaC. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Increase in the level of mRNA for 3-hydroxyanthranilate 3,4-dioxygenase in brain of epilepsy-prone El mice."
    Nakagawa Y., Asai H., Mori H., Kitoh J., Nakano K.
    Biosci. Biotechnol. Biochem. 59:2191-2192(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  3. "Molecular cloning and functional expression of human 3-hydroxyanthranilic-acid dioxygenase."
    Malherbe P., Kohler C., da Prada M., Lang G., Kiefer V., Schwarcz R., Lahm H., Cesura A.M.
    J. Biol. Chem. 269:13792-13797(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] OF 44-236, PARTIAL PROTEIN SEQUENCE.

Entry informationi

Entry namei3HAO_RAT
AccessioniPrimary (citable) accession number: P46953
Secondary accession number(s): P70474, Q5RKK0, Q64556
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 15, 1998
Last modified: September 3, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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