3-hydroxyanthranilate 3,4-dioxygenase - Rattus norvegicus (Rat)

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Reviewed, UniProtKB/Swiss-Prot P46953 (3HAO_RAT)

Last modified June 16, 2009. Version 66. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    3-hydroxyanthranilate 3,4-dioxygenase
    EC=1.13.11.6
Alternative name(s):
    3-hydroxyanthranilic acid dioxygenase
      Short name=HAD
    3-hydroxyanthranilate oxygenase
      Short name=3-HAO

Gene names

Name:

Haao

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate By similarity.
Catalytic activity	3-hydroxyanthranilate + O₂ = 2-amino-3-carboxymuconate semialdehyde.
Cofactor	Fe²⁺ ion By similarity.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; pyridine-2,3-dicarboxylate from L-kynurenine: step 3/3.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the 3-HAO family.

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Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW quinolinate metabolic process Inferred from direct assay. Source: RGD
Cellular component	mitochondrial membrane Inferred from direct assay. Source: RGD soluble fraction Inferred from direct assay. Source: RGD
Molecular function	3-hydroxyanthranilate 3,4-dioxygenase activity Inferred from direct assay. Source: RGD iron ion binding Inferred from direct assay. Source: RGD oxygen binding Inferred from direct assay. Source: RGD
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 286

286

3-hydroxyanthranilate 3,4-dioxygenase

PRO_0000064374

Sites

Metal binding

Iron; catalytic By similarity

Metal binding

Iron; catalytic By similarity

Metal binding

Iron; catalytic By similarity

Binding site

Dioxygen By similarity

Binding site

Substrate By similarity

Binding site

Substrate By similarity

Binding site

105

Substrate By similarity

Amino acid modifications

Modified residue

Phosphoserine By similarity

Experimental info

Sequence conflict

R → Q in AAH85739. Ref.2

Sequence conflict

K → S AA sequence Ref.3

Sequence conflict

199

S → F AA sequence Ref.3

Sequence conflict

204

G → C AA sequence Ref.3

Sequence conflict

214

H → Y AA sequence Ref.3

Sequence conflict

229

W → P AA sequence Ref.3

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Sequences

Sequence

Length

Mass (Da)

Tools

P46953-1 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: B4F535AD8949DAB7
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FASTA

286

32,582

        10         20         30         40         50         60 
MERCVRVKSW VEENRASFQP PVCNKLMHRE QLKIMFVGGP NTRKDYHIEE GEEVFYQLEG 

        70         80         90        100        110        120 
DMVLRVLEQG EHRDVVIRQG EIFLLPARVP HSPQRFANTM GLVIERRRME TELDGLRYYV 

       130        140        150        160        170        180 
GDTEDVLFEK WFHCKDLGTQ LAPIIQEFFH SEQYRTGKPN PDQLLKEPPF PLSTRSVMEP 

       190        200        210        220        230        240 
MSLKAWLESH SRELQAGTSL SLFGDSYETQ VIAHGQGSSK GPRQDVDVWL WQLEGSSKVT 

       250        260        270        280 
MGGQCVALAP DDSLLVPAGF SYMWERAQGS VALSVTQDPA CKKPLG

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Increase in the level of mRNA for 3-hydroxyanthranilate 3,4-dioxygenase in brain of epilepsy-prone El mice." Nakagawa Y., Asai H., Mori H., Kitoh J., Nakano K. Biosci. Biotechnol. Biochem. 59:2191-2192(1995) [PubMed: 8541664] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	"Molecular cloning and functional expression of human 3-hydroxyanthranilic-acid dioxygenase." Malherbe P., Kohler C., da Prada M., Lang G., Kiefer V., Schwarcz R., Lahm H., Cesura A.M. J. Biol. Chem. 269:13792-13797(1994) [PubMed: 7514594] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] OF 44-236, PARTIAL PROTEIN SEQUENCE.

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Cross-references

Sequence databases
	D44494 mRNA. Translation: BAA07937.1. BC085739 mRNA. Translation: AAH85739.1. D28339 mRNA. Translation: BAA21019.1.
IPI	IPI00339188.
RefSeq	NP_064461.1.
UniGene	Rn.48675
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P46953.
Genome annotation databases
Ensembl	ENSRNOG00000031263. Rattus norvegicus. [Contig view]
GeneID	56823.
KEGG	rno:56823.
Organism-specific databases
RGD	71071. Haao.
Phylogenomic databases
HOVERGEN	P46953.
Enzyme and pathway databases
BRENDA	1.13.11.6. 248.
Gene expression databases
ArrayExpress	P46953.
GermOnline	ENSRNOG00000031263. Rattus norvegicus.
Family and domain databases
InterPro	IPR010329. 3hydroanth_dOase. IPR016700. 3hydroanth_dOase_met. [Graphical view]
Pfam	PF06052. 3-HAO. 1 hit. [Graphical view]
PIRSF	PIRSF017681. 3hydroanth_dOase_animal. 1 hit.
TIGRFAMs	TIGR03037. anthran_nbaC. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	611253.

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Entry information

Entry name

3HAO_RAT

Accession

Primary (citable) accession number: P46953
Secondary accession number(s): P70474, Q5RKK0, Q64556

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	December 15, 1998
Last modified:	June 16, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents