P46951 (YPP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cargo-transport protein YPP1

Alternative name(s):
Alpha-synuclein protective protein 1

Gene names

Name:	YPP1
Ordered Locus Names:	YGR198W
ORF Names:	G7594

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	817 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in endocytosis. Ref.8
Subunit structure	Interacts with STT4 and ribosomes. Ref.4 Ref.7
Subcellular location	Cytoplasmic granule. Cell membrane; Peripheral membrane protein; Cytoplasmic side Ref.4 Ref.5 Ref.8.
Miscellaneous	Present with 1640 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the YPP1 family.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cell membrane Membrane
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein targeting to vacuole Inferred from direct assay Ref.8. Source: SGD receptor-mediated endocytosis Inferred from direct assay Ref.8. Source: SGD response to pheromone Inferred from direct assay Ref.8. Source: SGD
Cellular component	actin cortical patch Inferred from direct assay Ref.8. Source: SGD cytosol Inferred from direct assay Ref.8. Source: SGD endosome Inferred from direct assay Ref.8. Source: SGD plasma membrane Inferred from direct assay Ref.4. Source: SGD
Molecular function	protein binding Inferred from physical interaction Ref.4. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
STT4	P37297	2	EBI-23455,EBI-18454

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 817	817	Cargo-transport protein YPP1		PRO_0000202843

Sequences

	Sequence		Length	Mass (Da)
	P46951 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: AC42730C8B9C3E4B	FASTA	817	95,366
	10 20 30 40 50 60 MPNSNVRIPP TVPSKIIDVV DQALRARLLG GSTFNSGFDS LDSVLNLQFR LHYHVIGSNG 70 80 90 100 110 120 PAKPVCDVLL KESQNLEKNM SMMEELNDYP EITKLVEKIL FNCLGILFFH RGQFQESQRC 130 140 150 160 170 180 LLHSLKIHNN TASQKTALME QYDRYLIVEN LYYRGLVSQD INIMQNVFYK ELLAHVDTIP 190 200 210 220 230 240 PESNGLLFEY ISLIVAKLRF NQIQDLAENF KTTVENPFIL FLYMIKKFQS PLKKHIDNDD 250 260 270 280 290 300 LYLKFGQNVL LKAKFPTASE TNDEALEHFN VFLQYYFKFT HIKKIKVNPS WYNFIISSME 310 320 330 340 350 360 KTFQSIEVSK TAMFLFQNLS DNSNDEIKKK TFKRESILNF VNFVKYNDKY YQLHDNSHRD 370 380 390 400 410 420 IISFIDAYSF ILQNSSKTDS IENVFDYDNT VSTFATSLNS FYKEYNLPLM SQSESLDWLE 430 440 450 460 470 480 NSTRCVYPGN ISKVLTNAWS TLYEIRKYQL DFLVSNNLTS YLCNAMMLST KEKDNADVEE 490 500 510 520 530 540 QEEGEEEKAL RELQFKYSYT LAQQRHIETA IKTLESLILS KNPNYYKAWH LLALCRSVQE 550 560 570 580 590 600 DKEMSYKIVC SVLEAMNESL QNNTLLLNDR WQFIHLKLTQ LALIEEIFGT LEALETLPEV 610 620 630 640 650 660 FELYATLFPD SQPELNSMGP KYSQTKEYLL QMVWIFAANM YMRTKDNDED AKAAIKEASN 670 680 690 700 710 720 VESKFKNLNC NIANGYLSII KDEPGVALKE FETVLYYDEN NLDALVGFAE LIFPEELGVE 730 740 750 760 770 780 ETNLERYYTL SLDKKPGKRA KLTFVNDTDR SAAYARLKFL LECAILESIE AYYSPEVWWY 790 800 810 LSLIYEKYQD DEYKNSLLKC IKYQELNPIR SLRYCNY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete sequence of a 9000 bp fragment of the right arm of Saccharomyces cerevisiae chromosome VII contains four previously unknown open reading frames." Guerreiro P., Maia e Silva A., Barreiros T., Arroyo J., Garcia-Gonzalez M., Garcia-Saez M.I., Rodrigues-Pousada C., Nombela C. Yeast 11:1087-1091(1995) [PubMed: 7502584] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Assigning function to yeast proteins by integration of technologies." Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M., Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E., Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N. Mol. Cell 12:1353-1365(2003) [PubMed: 14690591] [Abstract] Cited for: MASS SPECTROMETRY, INTERACTION WITH STT4, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes." Fleischer T.C., Weaver C.M., McAfee K.J., Jennings J.L., Link A.J. Genes Dev. 20:1294-1307(2006) [PubMed: 16702403] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH RIBOSOMES.
[8]	"YGR198w (YPP1) targets A30P alpha-synuclein to the vacuole for degradation." Flower T.R., Clark-Dixon C., Metoyer C., Yang H., Shi R., Zhang Z., Witt S.N. J. Cell Biol. 177:1091-1104(2007) [PubMed: 17576801] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X82775 Genomic DNA. Translation: CAA58017.1. Z72983 Genomic DNA. Translation: CAA97225.1. BK006941 Genomic DNA. Translation: DAA08291.1.
PIR	S53921.
RefSeq	NP_011714.1. NM_001181327.1.
3D structure databases
ProteinModelPortal	P46951.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6350N.
IntAct	P46951. 22 interactions.
MINT	MINT-2492312.
STRING	P46951.
Proteomic databases
PeptideAtlas	P46951.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR198W; YGR198W; YGR198W.
GeneID	853112.
KEGG	sce:YGR198W.
NMPDR	fig|4932.3.peg.2841.
Organism-specific databases
CYGD	YGR198w.
SGD	S000003430. YPP1.
Phylogenomic databases
eggNOG	fuNOG04965.
HOGENOM	HBG204422.
OMA	FIHIKLT.
OrthoDB	EOG45QMNC.
Gene expression databases
ArrayExpress	P46951.
Genevestigator	P46951.
GermOnline	YGR198W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011990. TPR-like_helical. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 3 hits.
ProtoNet	Search...
Other
NextBio	973130.

Entry information

Entry name

YPP1_YEAST

Accession

Primary (citable) accession number: P46951
Secondary accession number(s): D6VUY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program