P46950 (SNG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitrosoguanidine resistance protein SNG1

Gene names

Name:	SNG1
Ordered Locus Names:	YGR197C
ORF Names:	G7591

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	547 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as a N-methyl-N'nitro-N-nitrosoguanidine (MNNG) export permease.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Induction	Transcriptionally regulated by PDR8. Ref.5
Sequence similarities	To yeast YJR015W.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleobase-containing compound transport Inferred from sequence model PubMed 20424846. Source: SGD
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589PubMed 20424846. Source: SGD plasma membrane Inferred from direct assay PubMed 20424846. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 547	547	Nitrosoguanidine resistance protein SNG1		PRO_0000072007
Regions
	Transmembrane	109 – 129	21	Helical; Potential
	Transmembrane	159 – 179	21	Helical; Potential
	Transmembrane	318 – 338	21	Helical; Potential
	Transmembrane	363 – 383	21	Helical; Potential
	Transmembrane	394 – 414	21	Helical; Potential
	Transmembrane	418 – 438	21	Helical; Potential
	Transmembrane	457 – 477	21	Helical; Potential
	Transmembrane	488 – 508	21	Helical; Potential
Amino acid modifications
	Modified residue	45	1	Phosphoserine Ref.7
	Modified residue	91	1	Phosphothreonine Ref.6 Ref.7
Experimental info
	Sequence conflict	117	1	A → V in CAA52881. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P46950 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D4421BA2DED55420	FASTA	547	61,293
	10 20 30 40 50 60 MTKSVGDEES QYIEDPSFAA AAAFTGGRDG VSYSNQRFAE GSGHSSDLAK SLEDYRPPDE 70 80 90 100 110 120 KPSSLSSVGE GGANEEEKGG NDGGPLARIQ TGLFSPRLRN HRKKILSKFV LNNFFIACVC 130 140 150 160 170 180 VSLISIYWGA CYGTDRYFFK VKNIVVLQDA PSNTSVQSIS AIIPSLLASV PGTWHIYNAT 190 200 210 220 230 240 SFHRKFGTTN STEIDRKIVD LIYDERYWLA LNVKPNATDT LYNSLISQDA NSEFNSSIFF 250 260 270 280 290 300 ESVFESGRDP SSVKSTILPL MQQLEVRLQK YYVKEYLPSL MSNITSNDRD LNINMENWAI 310 320 330 340 350 360 AGQLLFTYND YRPFADRILM APLQVGLIYC ILLTVLQLSL YGKLHGEMAR VLKPKHILIY 370 380 390 400 410 420 RLLISWATYF LLSIGFCTVS AIFRIDFTPA FGRGGFVVYW MSTWLVMMAV GGANENVLSL 430 440 450 460 470 480 VIAYCPPYLS IWLMTWIILN ISASFYPMVL NNEFYRYGYI MPIHNAVDIY KVIFLNLTKR 490 500 510 520 530 540 KMGRNYGILV AWVALNTSLM PFCMKFAGKK MQKNAMQAAE AAVAAATQRA SRPAEANTDK NNNPPGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SNG1 -- a new gene involved in nitrosoguanidine resistance in Saccharomyces cerevisiae." Grey M., Pich C.T., Haase E., Brendel M. Mutat. Res. 346:207-214(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete sequence of a 9000 bp fragment of the right arm of Saccharomyces cerevisiae chromosome VII contains four previously unknown open reading frames." Guerreiro P., Maia e Silva A., Barreiros T., Arroyo J., Garcia-Gonzalez M., Garcia-Saez M.I., Rodrigues-Pousada C., Nombela C. Yeast 11:1087-1091(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"A general strategy to uncover transcription factor properties identifies a new regulator of drug resistance in yeast." Hikkel I., Lucau-Danila A., Delaveau T., Marc P., Devaux F., Jacq C. J. Biol. Chem. 278:11427-11432(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-91, MASS SPECTROMETRY. Strain: ADR376.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45 AND THR-91, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X74920 Genomic DNA. Translation: CAA52881.1. X82775 Genomic DNA. Translation: CAA58016.1. Z72982 Genomic DNA. Translation: CAA97224.1. BK006941 Genomic DNA. Translation: DAA08290.1.
PIR	S53920.
RefSeq	NP_011713.1. NM_001181326.1.
3D structure databases
ProteinModelPortal	P46950.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-8148N.
IntAct	P46950. 1 interaction.
STRING	4932.YGR197C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR197C; YGR197C; YGR197C.
GeneID	853111.
KEGG	sce:YGR197C.
Organism-specific databases
CYGD	YGR197c.
SGD	S000003429. SNG1.
Phylogenomic databases
eggNOG	NOG42045.
GeneTree	ENSGT00390000015455.
HOGENOM	HOG000141871.
OMA	EFYRYGY.
OrthoDB	EOG4TF3V7.
Gene expression databases
Genevestigator	P46950.
GermOnline	YGR197C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022703. DUF3533. [Graphical view]
Pfam	PF12051. DUF3533. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973127.

Entry information

Entry name

SNG1_YEAST

Accession

Primary (citable) accession number: P46950
Secondary accession number(s): D6VUX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program