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P46947 (CWC26_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pre-mRNA-splicing factor CWC26
Alternative name(s):
Bud site selection protein 13
Complexed with CEF1 protein 26
Synthetic lethal with CLF1 protein 7
Gene names
Name:BUD13
Synonyms:CWC26, SLC7
Ordered Locus Names:YGL174W
ORF Names:G1642
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length266 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for efficient splicing and pre-mRNA nuclear retention. May also be involved in positioning the proximal bud pole signal. Ref.5 Ref.6 Ref.8 Ref.10

Subunit structure

Belongs to the pre-mRNA retention and splicing (RES) complex composed of at least BUD13, IST3 and PML1. May also belong to the CWC complex (or CEF1-associated complex) composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with IST3 and PML1. Ref.7 Ref.10

Subcellular location

Cytoplasm. Nucleus Ref.6 Ref.9.

Sequence similarities

Belongs to the CWC26 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

IST3P405655EBI-24073,EBI-25387

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 266266Pre-mRNA-splicing factor CWC26
PRO_0000079610

Regions

Compositional bias15 – 2511Poly-Lys

Sequences

Sequence LengthMass (Da)Tools
P46947 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 1584B390121325CD

FASTA26630,472
        10         20         30         40         50         60 
MALHQYLSET YGPTKPKNKT KKKKKESKSD ANSDKTSLIV KERLSTLQQE QEKSGVASFS 

        70         80         90        100        110        120 
KFDKQKSKNI WKNLETNELS HAITHPSASS ITGNESKNDL KEIRAQEPLV TVADKSKTRK 

       130        140        150        160        170        180 
TIYRDAQGHK IQEDSKIDDS SFSRSKYEDE KAAEREQYLK NLNMGDVQKL GINVDAHDKK 

       190        200        210        220        230        240 
KNQTASSLTI EDPAITFTHD KERTVKTSLL GRKLYDKPAP ENRFAIMPGS RWDGVHRSNG 

       250        260 
FEEKWFAKQN EINEKKVQSY TLQEDY 

« Hide

References

« Hide 'large scale' references
[1]"The sequence of an 11.1 kb fragment on the left arm of Saccharomyces cerevisiae chromosome VII reveals six open reading frames including NSP49, KEM1 and four putative new genes."
Bertani I., Coglievina M., Zaccaria P., Klima R., Bruschi C.V.
Yeast 11:1187-1194(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Genetic analysis of the bipolar pattern of bud site selection in the yeast Saccharomyces cerevisiae."
Zahner J.E., Harkins H.A., Pringle J.R.
Mol. Cell. Biol. 16:1857-1870(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae."
Ni L., Snyder M.
Mol. Biol. Cell 12:2147-2170(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[8]"Genetic interactions with CLF1 identify additional pre-mRNA splicing factors and a link between activators of yeast vesicular transport and splicing."
Vincent K., Wang Q., Jay S., Hobbs K., Rymond B.C.
Genetics 164:895-907(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]"Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing."
Dziembowski A., Ventura A.-P., Rutz B., Caspary F., Faux C., Halgand F., Laprevote O., Seraphin B.
EMBO J. 23:4847-4856(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH IST3 AND PML1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X84705 Genomic DNA. Translation: CAA59179.1.
Z72696 Genomic DNA. Translation: CAA96886.1.
AY558504 Genomic DNA. Translation: AAS56830.1.
BK006941 Genomic DNA. Translation: DAA07939.1.
PIRS59237.
RefSeqNP_011341.3. NM_001181039.3.

3D structure databases

ProteinModelPortalP46947.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33079. 177 interactions.
DIPDIP-2075N.
IntActP46947. 9 interactions.
MINTMINT-389797.
STRING4932.YGL174W.

Proteomic databases

MaxQBP46947.
PaxDbP46947.
PeptideAtlasP46947.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL174W; YGL174W; YGL174W.
GeneID852701.
KEGGsce:YGL174W.

Organism-specific databases

CYGDYGL174w.
SGDS000003142. BUD13.

Phylogenomic databases

eggNOGNOG317350.
HOGENOMHOG000112094.
KOK13106.
OMAETNELSH.
OrthoDBEOG7G7M2F.

Enzyme and pathway databases

BioCycYEAST:G3O-30662-MONOMER.

Gene expression databases

GenevestigatorP46947.

Family and domain databases

InterProIPR018609. Bud13.
[Graphical view]
PfamPF09736. Bud13. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972047.

Entry information

Entry nameCWC26_YEAST
AccessionPrimary (citable) accession number: P46947
Secondary accession number(s): D6VTX8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families