P46947 (CWC26_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor CWC26

Alternative name(s):
Bud site selection protein 13
Complexed with CEF1 protein 26
Synthetic lethal with CLF1 protein 7

Gene names

Name:	BUD13
Synonyms:	CWC26, SLC7
Ordered Locus Names:	YGL174W
ORF Names:	G1642

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for efficient splicing and pre-mRNA nuclear retention. May also be involved in positioning the proximal bud pole signal. Ref.5 Ref.6 Ref.8 Ref.10
Subunit structure	Belongs to the pre-mRNA retention and splicing (RES) complex composed of at least BUD13, IST3 and PML1. May also belong to the CWC complex (or CEF1-associated complex) composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with IST3 and PML1. Ref.7 Ref.10
Subcellular location	Cytoplasm. Nucleus Ref.6 Ref.9.
Sequence similarities	Belongs to the CWC26 family.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Cytoplasm Nucleus Spliceosome
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular bud site selection Inferred from mutant phenotype Ref.6. Source: SGD mRNA export from nucleus Inferred from mutant phenotype Ref.10. Source: SGD mRNA splicing, via spliceosome Inferred from direct assay Ref.10. Source: SGD
Cellular_component	RES complex Inferred from direct assay Ref.10. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell spliceosomal complex Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IST3	P40565	7	EBI-24073,EBI-25387

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Pre-mRNA-splicing factor CWC26		PRO_0000079610
Regions
	Compositional bias	15 – 25	11	Poly-Lys

Sequences

	Sequence		Length	Mass (Da)
	P46947 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 1584B390121325CD	FASTA	266	30,472
	10 20 30 40 50 60 MALHQYLSET YGPTKPKNKT KKKKKESKSD ANSDKTSLIV KERLSTLQQE QEKSGVASFS 70 80 90 100 110 120 KFDKQKSKNI WKNLETNELS HAITHPSASS ITGNESKNDL KEIRAQEPLV TVADKSKTRK 130 140 150 160 170 180 TIYRDAQGHK IQEDSKIDDS SFSRSKYEDE KAAEREQYLK NLNMGDVQKL GINVDAHDKK 190 200 210 220 230 240 KNQTASSLTI EDPAITFTHD KERTVKTSLL GRKLYDKPAP ENRFAIMPGS RWDGVHRSNG 250 260 FEEKWFAKQN EINEKKVQSY TLQEDY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of an 11.1 kb fragment on the left arm of Saccharomyces cerevisiae chromosome VII reveals six open reading frames including NSP49, KEM1 and four putative new genes." Bertani I., Coglievina M., Zaccaria P., Klima R., Bruschi C.V. Yeast 11:1187-1194(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Genetic analysis of the bipolar pattern of bud site selection in the yeast Saccharomyces cerevisiae." Zahner J.E., Harkins H.A., Pringle J.R. Mol. Cell. Biol. 16:1857-1870(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae." Ni L., Snyder M. Mol. Biol. Cell 12:2147-2170(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs." Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L. Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE CWC COMPLEX.
[8]	"Genetic interactions with CLF1 identify additional pre-mRNA splicing factors and a link between activators of yeast vesicular transport and splicing." Vincent K., Wang Q., Jay S., Hobbs K., Rymond B.C. Genetics 164:895-907(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing." Dziembowski A., Ventura A.-P., Rutz B., Caspary F., Faux C., Halgand F., Laprevote O., Seraphin B. EMBO J. 23:4847-4856(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MASS SPECTROMETRY, INTERACTION WITH IST3 AND PML1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X84705 Genomic DNA. Translation: CAA59179.1. Z72696 Genomic DNA. Translation: CAA96886.1. AY558504 Genomic DNA. Translation: AAS56830.1. BK006941 Genomic DNA. Translation: DAA07939.1.
PIR	S59237.
RefSeq	NP_011341.3. NM_001181039.3.
3D structure databases
ProteinModelPortal	P46947.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2075N.
IntAct	P46947. 12 interactions.
MINT	MINT-389797.
STRING	4932.YGL174W.
Proteomic databases
PaxDb	P46947.
PeptideAtlas	P46947.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL174W; YGL174W; YGL174W.
GeneID	852701.
KEGG	sce:YGL170C. sce:YGL174W.
Organism-specific databases
CYGD	YGL174w.
SGD	S000003142. BUD13.
Phylogenomic databases
eggNOG	NOG317350.
HOGENOM	HOG000112094.
KO	K13106.
OMA	PTIVANE.
OrthoDB	EOG4V72S9.
Gene expression databases
Genevestigator	P46947.
GermOnline	YGL174W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018609. Bud13. [Graphical view]
Pfam	PF09736. Bud13. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972047.

Entry information

Entry name

CWC26_YEAST

Accession

Primary (citable) accession number: P46947
Secondary accession number(s): D6VTX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program