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P46947

- CWC26_YEAST

UniProt

P46947 - CWC26_YEAST

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Protein

Pre-mRNA-splicing factor CWC26

Gene

BUD13

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Required for efficient splicing and pre-mRNA nuclear retention. May also be involved in positioning the proximal bud pole signal.4 Publications

GO - Biological processi

  1. mRNA export from nucleus Source: SGD
  2. mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-30662-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor CWC26
Alternative name(s):
Bud site selection protein 13
Complexed with CEF1 protein 26
Synthetic lethal with CLF1 protein 7
Gene namesi
Name:BUD13
Synonyms:CWC26, SLC7
Ordered Locus Names:YGL174W
ORF Names:G1642
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL174w.
SGDiS000003142. BUD13.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: SGD
  3. RES complex Source: SGD
  4. spliceosomal complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 266266Pre-mRNA-splicing factor CWC26PRO_0000079610Add
BLAST

Proteomic databases

MaxQBiP46947.
PaxDbiP46947.
PeptideAtlasiP46947.

Expressioni

Gene expression databases

GenevestigatoriP46947.

Interactioni

Subunit structurei

Belongs to the pre-mRNA retention and splicing (RES) complex composed of at least BUD13, IST3 and PML1. May also belong to the CWC complex (or CEF1-associated complex) composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with IST3 and PML1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
IST3P405655EBI-24073,EBI-25387

Protein-protein interaction databases

BioGridi33079. 178 interactions.
DIPiDIP-2075N.
IntActiP46947. 9 interactions.
MINTiMINT-389797.
STRINGi4932.YGL174W.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2MKCNMR-C215-245[»]
4UQTNMR-B222-256[»]
ProteinModelPortaliP46947.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi15 – 2511Poly-LysAdd
BLAST

Sequence similaritiesi

Belongs to the CWC26 family.Curated

Phylogenomic databases

eggNOGiNOG317350.
HOGENOMiHOG000112094.
InParanoidiP46947.
KOiK13106.
OMAiETNELSH.
OrthoDBiEOG7G7M2F.

Family and domain databases

InterProiIPR018609. Bud13.
[Graphical view]
PfamiPF09736. Bud13. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46947-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALHQYLSET YGPTKPKNKT KKKKKESKSD ANSDKTSLIV KERLSTLQQE
60 70 80 90 100
QEKSGVASFS KFDKQKSKNI WKNLETNELS HAITHPSASS ITGNESKNDL
110 120 130 140 150
KEIRAQEPLV TVADKSKTRK TIYRDAQGHK IQEDSKIDDS SFSRSKYEDE
160 170 180 190 200
KAAEREQYLK NLNMGDVQKL GINVDAHDKK KNQTASSLTI EDPAITFTHD
210 220 230 240 250
KERTVKTSLL GRKLYDKPAP ENRFAIMPGS RWDGVHRSNG FEEKWFAKQN
260
EINEKKVQSY TLQEDY
Length:266
Mass (Da):30,472
Last modified:November 1, 1995 - v1
Checksum:i1584B390121325CD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X84705 Genomic DNA. Translation: CAA59179.1.
Z72696 Genomic DNA. Translation: CAA96886.1.
AY558504 Genomic DNA. Translation: AAS56830.1.
BK006941 Genomic DNA. Translation: DAA07939.1.
PIRiS59237.
RefSeqiNP_011341.3. NM_001181039.3.

Genome annotation databases

EnsemblFungiiYGL174W; YGL174W; YGL174W.
GeneIDi852701.
KEGGisce:YGL174W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X84705 Genomic DNA. Translation: CAA59179.1 .
Z72696 Genomic DNA. Translation: CAA96886.1 .
AY558504 Genomic DNA. Translation: AAS56830.1 .
BK006941 Genomic DNA. Translation: DAA07939.1 .
PIRi S59237.
RefSeqi NP_011341.3. NM_001181039.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2MKC NMR - C 215-245 [» ]
4UQT NMR - B 222-256 [» ]
ProteinModelPortali P46947.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33079. 178 interactions.
DIPi DIP-2075N.
IntActi P46947. 9 interactions.
MINTi MINT-389797.
STRINGi 4932.YGL174W.

Proteomic databases

MaxQBi P46947.
PaxDbi P46947.
PeptideAtlasi P46947.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL174W ; YGL174W ; YGL174W .
GeneIDi 852701.
KEGGi sce:YGL174W.

Organism-specific databases

CYGDi YGL174w.
SGDi S000003142. BUD13.

Phylogenomic databases

eggNOGi NOG317350.
HOGENOMi HOG000112094.
InParanoidi P46947.
KOi K13106.
OMAi ETNELSH.
OrthoDBi EOG7G7M2F.

Enzyme and pathway databases

BioCyci YEAST:G3O-30662-MONOMER.

Miscellaneous databases

NextBioi 972047.

Gene expression databases

Genevestigatori P46947.

Family and domain databases

InterProi IPR018609. Bud13.
[Graphical view ]
Pfami PF09736. Bud13. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of an 11.1 kb fragment on the left arm of Saccharomyces cerevisiae chromosome VII reveals six open reading frames including NSP49, KEM1 and four putative new genes."
    Bertani I., Coglievina M., Zaccaria P., Klima R., Bruschi C.V.
    Yeast 11:1187-1194(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Genetic analysis of the bipolar pattern of bud site selection in the yeast Saccharomyces cerevisiae."
    Zahner J.E., Harkins H.A., Pringle J.R.
    Mol. Cell. Biol. 16:1857-1870(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae."
    Ni L., Snyder M.
    Mol. Biol. Cell 12:2147-2170(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
    Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
    Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  8. "Genetic interactions with CLF1 identify additional pre-mRNA splicing factors and a link between activators of yeast vesicular transport and splicing."
    Vincent K., Wang Q., Jay S., Hobbs K., Rymond B.C.
    Genetics 164:895-907(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. "Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing."
    Dziembowski A., Ventura A.-P., Rutz B., Caspary F., Faux C., Halgand F., Laprevote O., Seraphin B.
    EMBO J. 23:4847-4856(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH IST3 AND PML1.

Entry informationi

Entry nameiCWC26_YEAST
AccessioniPrimary (citable) accession number: P46947
Secondary accession number(s): D6VTX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3