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P46947

- CWC26_YEAST

UniProt

P46947 - CWC26_YEAST

Protein

Pre-mRNA-splicing factor CWC26

Gene

BUD13

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Required for efficient splicing and pre-mRNA nuclear retention. May also be involved in positioning the proximal bud pole signal.4 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. mRNA export from nucleus Source: SGD
    2. mRNA splicing, via spliceosome Source: SGD

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30662-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-mRNA-splicing factor CWC26
    Alternative name(s):
    Bud site selection protein 13
    Complexed with CEF1 protein 26
    Synthetic lethal with CLF1 protein 7
    Gene namesi
    Name:BUD13
    Synonyms:CWC26, SLC7
    Ordered Locus Names:YGL174W
    ORF Names:G1642
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL174w.
    SGDiS000003142. BUD13.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: SGD
    3. RES complex Source: SGD
    4. spliceosomal complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 266266Pre-mRNA-splicing factor CWC26PRO_0000079610Add
    BLAST

    Proteomic databases

    MaxQBiP46947.
    PaxDbiP46947.
    PeptideAtlasiP46947.

    Expressioni

    Gene expression databases

    GenevestigatoriP46947.

    Interactioni

    Subunit structurei

    Belongs to the pre-mRNA retention and splicing (RES) complex composed of at least BUD13, IST3 and PML1. May also belong to the CWC complex (or CEF1-associated complex) composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with IST3 and PML1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    IST3P405655EBI-24073,EBI-25387

    Protein-protein interaction databases

    BioGridi33079. 177 interactions.
    DIPiDIP-2075N.
    IntActiP46947. 9 interactions.
    MINTiMINT-389797.
    STRINGi4932.YGL174W.

    Structurei

    3D structure databases

    ProteinModelPortaliP46947.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi15 – 2511Poly-LysAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CWC26 family.Curated

    Phylogenomic databases

    eggNOGiNOG317350.
    HOGENOMiHOG000112094.
    KOiK13106.
    OMAiETNELSH.
    OrthoDBiEOG7G7M2F.

    Family and domain databases

    InterProiIPR018609. Bud13.
    [Graphical view]
    PfamiPF09736. Bud13. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46947-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALHQYLSET YGPTKPKNKT KKKKKESKSD ANSDKTSLIV KERLSTLQQE    50
    QEKSGVASFS KFDKQKSKNI WKNLETNELS HAITHPSASS ITGNESKNDL 100
    KEIRAQEPLV TVADKSKTRK TIYRDAQGHK IQEDSKIDDS SFSRSKYEDE 150
    KAAEREQYLK NLNMGDVQKL GINVDAHDKK KNQTASSLTI EDPAITFTHD 200
    KERTVKTSLL GRKLYDKPAP ENRFAIMPGS RWDGVHRSNG FEEKWFAKQN 250
    EINEKKVQSY TLQEDY 266
    Length:266
    Mass (Da):30,472
    Last modified:November 1, 1995 - v1
    Checksum:i1584B390121325CD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84705 Genomic DNA. Translation: CAA59179.1.
    Z72696 Genomic DNA. Translation: CAA96886.1.
    AY558504 Genomic DNA. Translation: AAS56830.1.
    BK006941 Genomic DNA. Translation: DAA07939.1.
    PIRiS59237.
    RefSeqiNP_011341.3. NM_001181039.3.

    Genome annotation databases

    EnsemblFungiiYGL174W; YGL174W; YGL174W.
    GeneIDi852701.
    KEGGisce:YGL174W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84705 Genomic DNA. Translation: CAA59179.1 .
    Z72696 Genomic DNA. Translation: CAA96886.1 .
    AY558504 Genomic DNA. Translation: AAS56830.1 .
    BK006941 Genomic DNA. Translation: DAA07939.1 .
    PIRi S59237.
    RefSeqi NP_011341.3. NM_001181039.3.

    3D structure databases

    ProteinModelPortali P46947.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33079. 177 interactions.
    DIPi DIP-2075N.
    IntActi P46947. 9 interactions.
    MINTi MINT-389797.
    STRINGi 4932.YGL174W.

    Proteomic databases

    MaxQBi P46947.
    PaxDbi P46947.
    PeptideAtlasi P46947.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL174W ; YGL174W ; YGL174W .
    GeneIDi 852701.
    KEGGi sce:YGL174W.

    Organism-specific databases

    CYGDi YGL174w.
    SGDi S000003142. BUD13.

    Phylogenomic databases

    eggNOGi NOG317350.
    HOGENOMi HOG000112094.
    KOi K13106.
    OMAi ETNELSH.
    OrthoDBi EOG7G7M2F.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30662-MONOMER.

    Miscellaneous databases

    NextBioi 972047.

    Gene expression databases

    Genevestigatori P46947.

    Family and domain databases

    InterProi IPR018609. Bud13.
    [Graphical view ]
    Pfami PF09736. Bud13. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The sequence of an 11.1 kb fragment on the left arm of Saccharomyces cerevisiae chromosome VII reveals six open reading frames including NSP49, KEM1 and four putative new genes."
      Bertani I., Coglievina M., Zaccaria P., Klima R., Bruschi C.V.
      Yeast 11:1187-1194(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Genetic analysis of the bipolar pattern of bud site selection in the yeast Saccharomyces cerevisiae."
      Zahner J.E., Harkins H.A., Pringle J.R.
      Mol. Cell. Biol. 16:1857-1870(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae."
      Ni L., Snyder M.
      Mol. Biol. Cell 12:2147-2170(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
      Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
      Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    8. "Genetic interactions with CLF1 identify additional pre-mRNA splicing factors and a link between activators of yeast vesicular transport and splicing."
      Vincent K., Wang Q., Jay S., Hobbs K., Rymond B.C.
      Genetics 164:895-907(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. "Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing."
      Dziembowski A., Ventura A.-P., Rutz B., Caspary F., Faux C., Halgand F., Laprevote O., Seraphin B.
      EMBO J. 23:4847-4856(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH IST3 AND PML1.

    Entry informationi

    Entry nameiCWC26_YEAST
    AccessioniPrimary (citable) accession number: P46947
    Secondary accession number(s): D6VTX8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3