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Protein

Pre-mRNA-splicing factor CWC26

Gene

BUD13

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for efficient splicing and pre-mRNA nuclear retention. May also be involved in positioning the proximal bud pole signal.4 Publications

GO - Biological processi

  • mRNA export from nucleus Source: SGD
  • mRNA splicing, via spliceosome Source: SGD

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-30662-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor CWC26
Alternative name(s):
Bud site selection protein 13
Complexed with CEF1 protein 26
Synthetic lethal with CLF1 protein 7
Gene namesi
Name:BUD13
Synonyms:CWC26, SLC7
Ordered Locus Names:YGL174W
ORF Names:G1642
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL174W.
SGDiS000003142. BUD13.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000796101 – 266Pre-mRNA-splicing factor CWC26Add BLAST266

Proteomic databases

MaxQBiP46947.
PRIDEiP46947.

Interactioni

Subunit structurei

Belongs to the pre-mRNA retention and splicing (RES) complex composed of at least BUD13, IST3 and PML1. May also belong to the CWC complex (or CEF1-associated complex) composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with IST3 and PML1.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33079. 372 interactors.
DIPiDIP-2075N.
IntActiP46947. 18 interactors.
MINTiMINT-389797.
STRINGi4932.YGL174W.

Structurei

Secondary structure

1266
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi230 – 232Combined sources3
Helixi243 – 250Combined sources8
Beta strandi252 – 254Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MKCNMR-C215-245[»]
2MY2NMR-B215-255[»]
4UQTNMR-B222-256[»]
5GM6electron microscopy3.50W1-266[»]
5LQWelectron microscopy5.80L1-266[»]
ProteinModelPortaliP46947.
SMRiP46947.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi15 – 25Poly-LysAdd BLAST11

Sequence similaritiesi

Belongs to the CWC26 family.Curated

Phylogenomic databases

HOGENOMiHOG000112094.
InParanoidiP46947.
KOiK13106.
OMAiETNELSH.
OrthoDBiEOG092C5DZC.

Family and domain databases

InterProiView protein in InterPro
IPR018609. Bud13.
PfamiView protein in Pfam
PF09736. Bud13. 1 hit.

Sequencei

Sequence statusi: Complete.

P46947-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALHQYLSET YGPTKPKNKT KKKKKESKSD ANSDKTSLIV KERLSTLQQE
60 70 80 90 100
QEKSGVASFS KFDKQKSKNI WKNLETNELS HAITHPSASS ITGNESKNDL
110 120 130 140 150
KEIRAQEPLV TVADKSKTRK TIYRDAQGHK IQEDSKIDDS SFSRSKYEDE
160 170 180 190 200
KAAEREQYLK NLNMGDVQKL GINVDAHDKK KNQTASSLTI EDPAITFTHD
210 220 230 240 250
KERTVKTSLL GRKLYDKPAP ENRFAIMPGS RWDGVHRSNG FEEKWFAKQN
260
EINEKKVQSY TLQEDY
Length:266
Mass (Da):30,472
Last modified:November 1, 1995 - v1
Checksum:i1584B390121325CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84705 Genomic DNA. Translation: CAA59179.1.
Z72696 Genomic DNA. Translation: CAA96886.1.
AY558504 Genomic DNA. Translation: AAS56830.1.
BK006941 Genomic DNA. Translation: DAA07939.1.
PIRiS59237.
RefSeqiNP_011341.3. NM_001181039.3.

Genome annotation databases

EnsemblFungiiYGL174W; YGL174W; YGL174W.
GeneIDi852701.
KEGGisce:YGL174W.

Similar proteinsi

Entry informationi

Entry nameiCWC26_YEAST
AccessioniPrimary (citable) accession number: P46947
Secondary accession number(s): D6VTX8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 27, 2017
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names