Reviewed, UniProtKB/Swiss-Prot P46946 (SAE2_YEAST)

Names and origin

Protein names

Recommended name:
    Protein SAE2

Gene names

Name:	SAE2
Ordered Locus Names:	YGL175C
ORF Names:	G1639

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for meiotic recombination.
Miscellaneous	Present with 1030 molecules/cell in log phase SD medium. Ref.4

Ontologies

Keywords
Biological process	Meiosis
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA catabolic process, endonucleolytic Inferred from direct assay. Source: SGD gene conversion at mating-type locus, DNA double-strand break processing Inferred from mutant phenotype. Source: SGD meiosis Inferred from electronic annotation. Source: UniProtKB-KW meiotic DNA double-strand break processing Ref.1 Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	double-stranded DNA binding Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct single-stranded DNA specific endodeoxyribonuclease activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SRP1	Q02821	1	EBI-16440,EBI-1797

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Protein SAE2		PRO_0000097565
Amino acid modifications
	Modified residue	177	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P46946-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 284D57A3C11DD92B	FASTA	345	40,097
	10 20 30 40 50 60 MVTGEENVYL KSSLSILKEL SLDELLNVQY DVTTLIAKRV QALQNRNKCV LEEPNSKLAE 70 80 90 100 110 120 ILCHEKNAPQ QSSQTSAGPG EQDSEDFILT QFDEDIKKES AEVHYRNENK HTVQLPLVTM 130 140 150 160 170 180 PPNRHKRKIS EFSSPLNGLN NLSDLEDCSD TVIHEKDNDK ENKTRKLLGI ELENPESTSP 190 200 210 220 230 240 NLYKNVKDNF LFDFNTNPLT KRAWILEDFR PNEDIAPVKR GRRKLERFYA QVGKPEDSKH 250 260 270 280 290 300 RSLSVVIESQ NSDYEFAFDN LRNRSKSPPG FGRLDFPSTQ EGNEDKKKSQ EIIRRKTKYR 310 320 330 340 FLMASNNKIP PYEREYVFKR EQLNQIVDDG CFFWSDKLLQ IYARC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A general method for identifying recessive diploid-specific mutations in Saccharomyces cerevisiae, its application to the isolation of mutants blocked at intermediate stages of meiotic prophase and characterization of a new gene SAE2." McKee A.H.Z., Kleckner N. Genetics 146:797-816(1997) [PubMed: 9215888] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The sequence of an 11.1 kb fragment on the left arm of Saccharomyces cerevisiae chromosome VII reveals six open reading frames including NSP49, KEM1 and four putative new genes." Bertani I., Coglievina M., Zaccaria P., Klima R., Bruschi C.V. Yeast 11:1187-1194(1995) [PubMed: 8619317] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-177, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U49447 Genomic DNA. Translation: AAB96338.1. X84705 Genomic DNA. Translation: CAA59178.1. Z72697 Genomic DNA. Translation: CAA96887.1.
PIR	S59236.
RefSeq	NP_011340.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1603N.
IntAct	P46946. 5 interactions.
Genome annotation databases
Ensembl	YGL175C. Saccharomyces cerevisiae. [Contig view]
GeneID	852700.
GenomeReviews	Gene locus YGL175C in contig Y13135_GR.
KEGG	sce:YGL175C.
NMPDR	fig|4932.3.peg.2444.
Organism-specific databases
CYGD	YGL175c.
SGD	S000003143. SAE2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P46946.
Gene expression databases
GermOnline	YGL175C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013882. DNA-repair_Sae2/CtIP. [Graphical view]
Pfam	PF08573. SAE2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	972044.

Entry information

Entry name

SAE2_YEAST

Accession

Primary (citable) accession number: P46946

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)