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Protein

Translation factor GUF1, mitochondrial

Gene

GUF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.UniRule annotation1 Publication

Miscellaneous

Present with 1890 molecules/cell in log phase SD medium.1 Publication
This protein may be expected to contain an N-terminal transit peptide but none has been predicted.UniRule annotation

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi53 – 60GTPUniRule annotation8
Nucleotide bindingi120 – 124GTPUniRule annotation5
Nucleotide bindingi174 – 177GTPUniRule annotation4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW
  • ribosome binding Source: SGD

GO - Biological processi

  • positive regulation of translation Source: SGD
  • translation Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32384-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Translation factor GUF1, mitochondrialUniRule annotation (EC:3.6.5.-)
Alternative name(s):
Elongation factor 4 homologUniRule annotation
Short name:
EF-4UniRule annotation
GTPase GUF1UniRule annotation
Ribosomal back-translocaseUniRule annotation
Gene namesi
Name:GUF1UniRule annotation
Ordered Locus Names:YLR289W
ORF Names:L8003.7
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR289W
SGDiS000004280 GUF1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1250406

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000915601 – 645Translation factor GUF1, mitochondrialAdd BLAST645

Proteomic databases

MaxQBiP46943
PaxDbiP46943
PRIDEiP46943

Interactioni

Protein-protein interaction databases

BioGridi31555, 55 interactors
DIPiDIP-2842N
IntActiP46943, 5 interactors
MINTiP46943
STRINGi4932.YLR289W

Chemistry databases

BindingDBiP46943

Structurei

3D structure databases

ProteinModelPortaliP46943
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 228tr-type GAdd BLAST185

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074940
HOGENOMiHOG000020624
InParanoidiP46943
KOiK21594
OMAiALIFDSW
OrthoDBiEOG092C1LMV

Family and domain databases

CDDicd03709 lepA_C, 1 hit
Gene3Di3.30.70.2570, 1 hit
HAMAPiMF_00071 LepA, 1 hit
InterProiView protein in InterPro
IPR006297 EF-4
IPR035647 EFG_III/V
IPR000640 EFG_V-like
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR038363 LepA_C_sf
IPR013842 LepA_CTD
IPR035654 LepA_IV
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
PfamiView protein in Pfam
PF00679 EFG_C, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF06421 LepA_C, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF52540 SSF52540, 1 hit
SSF54980 SSF54980, 2 hits
TIGRFAMsiTIGR01393 lepA, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P46943-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKFRIRPVR HIRCYKRHAY FLRYNHTTTP AQKLQAQIEQ IPLENYRNFS
60 70 80 90 100
IVAHVDHGKS TLSDRLLEIT HVIDPNARNK QVLDKLEVER ERGITIKAQT
110 120 130 140 150
CSMFYKDKRT GKNYLLHLID TPGHVDFRGE VSRSYASCGG AILLVDASQG
160 170 180 190 200
IQAQTVANFY LAFSLGLKLI PVINKIDLNF TDVKQVKDQI VNNFELPEED
210 220 230 240 250
IIGVSAKTGL NVEELLLPAI IDRIPPPTGR PDKPFRALLV DSWYDAYLGA
260 270 280 290 300
VLLVNIVDGS VRKNDKVICA QTKEKYEVKD IGIMYPDRTS TGTLKTGQVG
310 320 330 340 350
YLVLGMKDSK EAKIGDTIMH LSKVNETEVL PGFEEQKPMV FVGAFPADGI
360 370 380 390 400
EFKAMDDDMS RLVLNDRSVT LERETSNALG QGWRLGFLGS LHASVFRERL
410 420 430 440 450
EKEYGSKLII TQPTVPYLVE FTDGKKKLIT NPDEFPDGAT KRVNVAAFHE
460 470 480 490 500
PFIEAVMTLP QEYLGSVIRL CDSNRGEQID ITYLNTNGQV MLKYYLPLSH
510 520 530 540 550
LVDDFFGKLK SVSRGFASLD YEDAGYRISD VVKLQLLVNG NAIDALSRVL
560 570 580 590 600
HKSEVERVGR EWVKKFKEYV KSQLYEVVIQ ARANNKIIAR ETIKARRKDV
610 620 630 640
LQKLHASDVS RRKKLLAKQK EGKKHMKTVG NIQINQEAYQ AFLRR
Length:645
Mass (Da):73,188
Last modified:November 1, 1997 - v2
Checksum:iBA1C371CCFECDC9B
GO

Sequence cautioni

The sequence AAA96351 differs from that shown. Reason: Frameshift at position 578.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti260S → F in AAA96351 (PubMed:8553703).Curated1
Sequence conflicti354A → P in AAA96351 (PubMed:8553703).Curated1
Sequence conflicti374E → Q in AAA96351 (PubMed:8553703).Curated1
Sequence conflicti559G → R in AAA96351 (PubMed:8553703).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22360 Genomic DNA Translation: AAA96351.1 Frameshift.
U17243 Genomic DNA Translation: AAB67335.1
BK006945 Genomic DNA Translation: DAA09601.1
PIRiS50374
RefSeqiNP_013392.1, NM_001182177.1

Genome annotation databases

EnsemblFungiiYLR289W; YLR289W; YLR289W
GeneIDi850996
KEGGisce:YLR289W

Similar proteinsi

Entry informationi

Entry nameiGUF1_YEAST
AccessioniPrimary (citable) accession number: P46943
Secondary accession number(s): D6VYT5, Q05891
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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