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Protein

Translation factor GUF1, mitochondrial

Gene

GUF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.UniRule annotation1 Publication

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi53 – 608GTPUniRule annotation
Nucleotide bindingi120 – 1245GTPUniRule annotation
Nucleotide bindingi174 – 1774GTPUniRule annotation

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-HAMAP
  • ribosome binding Source: SGD

GO - Biological processi

  • positive regulation of translation Source: SGD
  • translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32384-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Translation factor GUF1, mitochondrialUniRule annotation (EC:3.6.5.-)
Alternative name(s):
Elongation factor 4 homologUniRule annotation
Short name:
EF-4UniRule annotation
GTPase GUF1UniRule annotation
Ribosomal back-translocaseUniRule annotation
Gene namesi
Name:GUF1UniRule annotation
Ordered Locus Names:YLR289W
ORF Names:L8003.7
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR289W.
SGDiS000004280. GUF1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial inner membrane Source: UniProtKB-SubCell
  • mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1250406.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 645Translation factor GUF1, mitochondrialPRO_0000091560
Transit peptidei1 – ?MitochondrionUniRule annotation

Proteomic databases

MaxQBiP46943.

Interactioni

Protein-protein interaction databases

BioGridi31555. 17 interactions.
DIPiDIP-2842N.
IntActiP46943. 1 interaction.
MINTiMINT-688936.

Chemistry

BindingDBiP46943.

Structurei

3D structure databases

ProteinModelPortaliP46943.
SMRiP46943. Positions 44-597.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 228185tr-type GAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00550000074940.
HOGENOMiHOG000020624.
InParanoidiP46943.
OMAiKPMVFCG.
OrthoDBiEOG7QNVVM.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46943-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKFRIRPVR HIRCYKRHAY FLRYNHTTTP AQKLQAQIEQ IPLENYRNFS
60 70 80 90 100
IVAHVDHGKS TLSDRLLEIT HVIDPNARNK QVLDKLEVER ERGITIKAQT
110 120 130 140 150
CSMFYKDKRT GKNYLLHLID TPGHVDFRGE VSRSYASCGG AILLVDASQG
160 170 180 190 200
IQAQTVANFY LAFSLGLKLI PVINKIDLNF TDVKQVKDQI VNNFELPEED
210 220 230 240 250
IIGVSAKTGL NVEELLLPAI IDRIPPPTGR PDKPFRALLV DSWYDAYLGA
260 270 280 290 300
VLLVNIVDGS VRKNDKVICA QTKEKYEVKD IGIMYPDRTS TGTLKTGQVG
310 320 330 340 350
YLVLGMKDSK EAKIGDTIMH LSKVNETEVL PGFEEQKPMV FVGAFPADGI
360 370 380 390 400
EFKAMDDDMS RLVLNDRSVT LERETSNALG QGWRLGFLGS LHASVFRERL
410 420 430 440 450
EKEYGSKLII TQPTVPYLVE FTDGKKKLIT NPDEFPDGAT KRVNVAAFHE
460 470 480 490 500
PFIEAVMTLP QEYLGSVIRL CDSNRGEQID ITYLNTNGQV MLKYYLPLSH
510 520 530 540 550
LVDDFFGKLK SVSRGFASLD YEDAGYRISD VVKLQLLVNG NAIDALSRVL
560 570 580 590 600
HKSEVERVGR EWVKKFKEYV KSQLYEVVIQ ARANNKIIAR ETIKARRKDV
610 620 630 640
LQKLHASDVS RRKKLLAKQK EGKKHMKTVG NIQINQEAYQ AFLRR
Length:645
Mass (Da):73,188
Last modified:November 1, 1997 - v2
Checksum:iBA1C371CCFECDC9B
GO

Sequence cautioni

The sequence AAA96351.1 differs from that shown. Reason: Frameshift at position 578. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti260 – 2601S → F in AAA96351 (PubMed:8553703).Curated
Sequence conflicti354 – 3541A → P in AAA96351 (PubMed:8553703).Curated
Sequence conflicti374 – 3741E → Q in AAA96351 (PubMed:8553703).Curated
Sequence conflicti559 – 5591G → R in AAA96351 (PubMed:8553703).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22360 Genomic DNA. Translation: AAA96351.1. Frameshift.
U17243 Genomic DNA. Translation: AAB67335.1.
BK006945 Genomic DNA. Translation: DAA09601.1.
PIRiS50374.
RefSeqiNP_013392.1. NM_001182177.1.

Genome annotation databases

EnsemblFungiiYLR289W; YLR289W; YLR289W.
GeneIDi850996.
KEGGisce:YLR289W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22360 Genomic DNA. Translation: AAA96351.1. Frameshift.
U17243 Genomic DNA. Translation: AAB67335.1.
BK006945 Genomic DNA. Translation: DAA09601.1.
PIRiS50374.
RefSeqiNP_013392.1. NM_001182177.1.

3D structure databases

ProteinModelPortaliP46943.
SMRiP46943. Positions 44-597.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31555. 17 interactions.
DIPiDIP-2842N.
IntActiP46943. 1 interaction.
MINTiMINT-688936.

Chemistry

BindingDBiP46943.
ChEMBLiCHEMBL1250406.

Proteomic databases

MaxQBiP46943.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR289W; YLR289W; YLR289W.
GeneIDi850996.
KEGGisce:YLR289W.

Organism-specific databases

EuPathDBiFungiDB:YLR289W.
SGDiS000004280. GUF1.

Phylogenomic databases

GeneTreeiENSGT00550000074940.
HOGENOMiHOG000020624.
InParanoidiP46943.
OMAiKPMVFCG.
OrthoDBiEOG7QNVVM.

Enzyme and pathway databases

BioCyciYEAST:G3O-32384-MONOMER.

Miscellaneous databases

PROiP46943.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "GUF1, a gene encoding a novel evolutionarily conserved GTPase in budding yeast."
    Kiser G.L., Weinert T.L.
    Yeast 11:1311-1316(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204626 / S288c / A364A.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Microarray-based method for monitoring yeast overexpression strains reveals small-molecule targets in TOR pathway."
    Butcher R.A., Bhullar B.S., Perlstein E.O., Marsischky G., LaBaer J., Schreiber S.L.
    Nat. Chem. Biol. 2:103-109(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY.
  7. "The membrane-bound GTPase Guf1 promotes mitochondrial protein synthesis under suboptimal conditions."
    Bauerschmitt H., Funes S., Herrmann J.M.
    J. Biol. Chem. 283:17139-17146(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGUF1_YEAST
AccessioniPrimary (citable) accession number: P46943
Secondary accession number(s): D6VYT5, Q05891
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: July 6, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1890 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.