65 kDa Yes-associated protein - Homo sapiens (Human)

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Reviewed, UniProtKB/Swiss-Prot P46937 (YAP1_HUMAN)

Last modified June 16, 2009. Version 88. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
65 kDa Yes-associated protein
Alternative name(s):
YAP65

Gene names

Name:	YAP1
Synonyms:	YAP65

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Subunit structure	Binds to the SH3 domain of the YES kinase. Binds to WBP1 and WBP2. Binds, in vitro, through the WW1 domain, to neural isoforms of ENAH that contain the PPSY motif By similarity.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8
Sequence similarities	Contains 1 WW domain.

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Ontologies

Keywords
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Molecular function	protein binding Ref.1 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 454

454

65 kDa Yes-associated protein

PRO_0000076071

Regions

Domain

171 – 204

Compositional bias

3 – 49

Pro-rich

Compositional bias

221 – 312

Gln-rich

Amino acid modifications

Modified residue

Phosphoserine Ref.8

Modified residue

109

Phosphoserine Ref.3 Ref.7

Modified residue

110

Phosphothreonine Ref.3 Ref.4

Modified residue

127

Phosphoserine Ref.8

Modified residue

128

Phosphoserine Ref.8

Modified residue

131

Phosphoserine Ref.8

Modified residue

138

Phosphoserine Ref.8

Modified residue

143

Phosphothreonine Ref.4 Ref.8

Modified residue

149

Phosphoserine Ref.8

Modified residue

154

Phosphothreonine Ref.4 Ref.8

Modified residue

236

Phosphoserine Ref.8

Modified residue

238

Phosphoserine Ref.5

Modified residue

251

Phosphoserine Ref.8

Modified residue

317

Phosphoserine Ref.8

Modified residue

321

Phosphoserine Ref.6

Secondary structure

454

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P46937-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 87CB840D3393EFC0
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FASTA

454

48,755

        10         20         30         40         50         60 
MDPGQQPPPQ PAPQGQGQPP SQPPQGQGPP SGPGQPAPAA TQAAPQAPPA GHQIVHVRGD 

        70         80         90        100        110        120 
SETDLEALFN AVMNPKTANV PQTVPMRLRK LPDSFFKPPE PKSHSRQAST DAGTAGALTP 

       130        140        150        160        170        180 
QHVRAHSSPA SLQLGAVSPG TLTPTGVVSG PAATPTAQHL RQSSFEIPDD VPLPAGWEMA 

       190        200        210        220        230        240 
KTSSGQRYFL NHIDQTTTWQ DPRKAMLSQM NVTAPTSPPV QQNMMNSASA MNQRISQSAP 

       250        260        270        280        290        300 
VKQPPPLAPQ SPQGGVMGGS NSNQQQQMRL QQLQMEKERL RLKQQELLRQ VRPQELALRS 

       310        320        330        340        350        360 
QLPTLEQDGG TQNPVSSPGM SQELRTMTTN SSDPFLNSGT YHSRDESTDS GLSMSSYSVP 

       370        380        390        400        410        420 
RTPDDFLNSV DEMDTGDTIN QSTLPSQQNR FPDYLEAIPG TNVDLGTLEG DGMNIEGEEL 

       430        440        450 
MPSLQEALSS DILNDMESVL AATKLDKESF LTWL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the mammalian YAP (Yes-associated protein) gene and its role in defining a novel protein module, the WW domain." Sudol M., Bork P., Einbond A., Kastury K., Druck T., Negrini M., Huebner K., Lehman D. J. Biol. Chem. 270:14733-14741(1995) [PubMed: 7782338] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Lung.
[2]	"Characterization of the WW domain of human Yes-associated protein and its polyproline containing ligands." Chen H.I., Einbond A., Kwak S.-J., Linn H., Koepf E., Peterson S., Kelly J.W., Sudol M. J. Biol. Chem. 272:17070-17077(1997) [PubMed: 9202023] [Abstract] Cited for: INTERACTION WITH WBP1 AND WBP2.
[3]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109 AND THR-110, MASS SPECTROMETRY. Tissue: Epithelium.
[4]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-110; THR-143 AND THR-154, MASS SPECTROMETRY. Tissue: Epithelium.
[5]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-238, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-321, MASS SPECTROMETRY.
[7]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109, MASS SPECTROMETRY.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-61; SER-127; SER-128; SER-131; SER-138; THR-143; SER-149; THR-154; SER-236; SER-251 AND SER-317, MASS SPECTROMETRY.
[9]	"Solution structures of the YAP65 WW domain and the variant L30 K in complex with the peptides GTPPPPYTVG, N-(n-octyl)-GPPPY and PLPPY and the application of peptide libraries reveal a minimal binding epitope." Pires J.R., Taha-Nejad F., Toepert F., Ast T., Hoffmueller U., Schneider-Mergener J., Kuehne R., Macias M.J., Oschkinat H. J. Mol. Biol. 314:1147-1156(2001) [PubMed: 11743730] [Abstract] Cited for: STRUCTURE BY NMR OF 165-210 OF WILD-TYPE AND MUTANT LYS-190 IN COMPLEX WITH PRO-RICH PEPTIDES.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

X80507 Genomic DNA. Translation: CAA56672.1.

IPI

IPI00009326.

PIR

A56954.

RefSeq

NP_006097.1.

UniGene

Hs.503692

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JMQ	NMR	-	A	165-210	[»]
1K5R	NMR	-	A	165-204	[»]
1K9Q	NMR	-	A	165-204	[»]
1K9R	NMR	-	A	165-204	[»]

SMR

P46937. Positions 165-210.

ModBase

Search...

Protein-protein interaction databases

IntAct

P46937. 1 interaction.

PTM databases

PhosphoSite

P46937.

Proteomic databases

PRIDE

P46937.

Genome annotation databases

Ensembl

ENSG00000137693. Homo sapiens. [Contig view]

GeneID

10413.

Organism-specific databases

GeneCards

GC11P101486.

H-InvDB

HIX0010062.

HGNC

HGNC:16262. YAP1.

HPA

CAB009370.

MIM

606608. gene.

PharmGKB

PA24564.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

P46937.

Enzyme and pathway databases

Pathway_Interaction_DB

tgfbrpathway. TGF-beta receptor signaling.

Gene expression databases

ArrayExpress

P46937.

Bgee

P46937.

CleanEx

HS_YAP1.

GermOnline

ENSG00000137693. Homo sapiens.

Family and domain databases

InterPro

IPR001202. WW_Rsp5_WWP.
[Graphical view]

Pfam

PF00397. WW. 1 hit.
[Graphical view]

SMART

SM00456. WW. 1 hit.
[Graphical view]

PROSITE

PS01159. WW_DOMAIN_1. 1 hit.
PS50020. WW_DOMAIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

39472.

SOURCE

Search...

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Entry information

Entry name

YAP1_HUMAN

Accession

Primary (citable) accession number: P46937

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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