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Protein

Glucosamine-6-phosphate isomerase 1

Gene

GNPDA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seems to trigger calcium oscillations in mammalian eggs. These oscillations serve as the essential trigger for egg activation and early development of the embryo (By similarity).By similarity

Catalytic activityi

Alpha-D-glucosamine 6-phosphate + H2O = D-fructose 6-phosphate + NH3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei72Proton acceptor; for enolization stepBy similarity1
Active sitei141For ring-opening stepBy similarity1
Active sitei143Proton acceptor; for ring-opening stepBy similarity1
Active sitei148For ring-opening stepBy similarity1

GO - Molecular functioni

  • glucosamine-6-phosphate deaminase activity Source: UniProtKB
  • hydrolase activity Source: UniProtKB-KW

GO - Biological processi

  • generation of precursor metabolites and energy Source: UniProtKB
  • glucosamine catabolic process Source: UniProtKB
  • glucose metabolic process Source: Reactome
  • N-acetylglucosamine catabolic process Source: GO_Central
  • N-acetylneuraminate catabolic process Source: GO_Central
  • single fertilization Source: ProtInc
  • UDP-N-acetylglucosamine biosynthetic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciZFISH:HS03692-MONOMER.
BRENDAi3.5.99.6. 2681.
ReactomeiR-HSA-70326. Glucose metabolism.
SABIO-RKP46926.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosamine-6-phosphate isomerase 1 (EC:3.5.99.6)
Alternative name(s):
Glucosamine-6-phosphate deaminase 1
Short name:
GNPDA 1
Short name:
GlcN6P deaminase 1
Oscillin
Gene namesi
Name:GNPDA1
Synonyms:GNPI, HLN, KIAA0060
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:4417. GNPDA1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi10007.
OpenTargetsiENSG00000113552.
PharmGKBiPA28796.

Polymorphism and mutation databases

BioMutaiGNPDA1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001601221 – 289Glucosamine-6-phosphate isomerase 1Add BLAST289

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei64N6-acetyllysineCombined sources1
Modified residuei161PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP46926.
MaxQBiP46926.
PaxDbiP46926.
PeptideAtlasiP46926.
PRIDEiP46926.

PTM databases

iPTMnetiP46926.
PhosphoSitePlusiP46926.

Expressioni

Gene expression databases

BgeeiENSG00000113552.
CleanExiHS_GNPDA1.
ExpressionAtlasiP46926. baseline and differential.
GenevisibleiP46926. HS.

Organism-specific databases

HPAiHPA000499.
HPA046891.

Interactioni

Subunit structurei

Homohexamer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MAGEA11P43364-23EBI-749356,EBI-10178634
PRTFDC1Q9NRG13EBI-749356,EBI-739759

Protein-protein interaction databases

BioGridi115325. 37 interactors.
IntActiP46926. 4 interactors.
STRINGi9606.ENSP00000311876.

Structurei

Secondary structure

1289
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 8Combined sources7
Helixi9 – 27Combined sources19
Beta strandi35 – 39Combined sources5
Helixi43 – 57Combined sources15
Beta strandi66 – 74Combined sources9
Helixi85 – 92Combined sources8
Helixi94 – 96Combined sources3
Helixi101 – 103Combined sources3
Helixi114 – 127Combined sources14
Beta strandi132 – 136Combined sources5
Beta strandi157 – 161Combined sources5
Helixi164 – 170Combined sources7
Helixi171 – 173Combined sources3
Turni174 – 176Combined sources3
Helixi178 – 180Combined sources3
Beta strandi183 – 187Combined sources5
Helixi190 – 194Combined sources5
Beta strandi199 – 203Combined sources5
Helixi206 – 208Combined sources3
Helixi209 – 216Combined sources8
Helixi225 – 231Combined sources7
Beta strandi233 – 240Combined sources8
Helixi241 – 244Combined sources4
Helixi249 – 257Combined sources9
Helixi259 – 262Combined sources4
Helixi263 – 265Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NE7X-ray1.75A/B/C/D/E/F1-289[»]
ProteinModelPortaliP46926.
SMRiP46926.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP46926.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3148. Eukaryota.
COG0363. LUCA.
GeneTreeiENSGT00390000014316.
HOGENOMiHOG000064979.
HOVERGENiHBG002546.
InParanoidiP46926.
KOiK02564.
PhylomeDBiP46926.
TreeFamiTF300841.

Family and domain databases

CDDicd01399. GlcN6P_deaminase. 1 hit.
HAMAPiMF_01241. GlcN6P_deamin. 1 hit.
InterProiIPR006148. Glc/Gal-6P_isomerase.
IPR004547. Glucosamine6P_isomerase.
IPR018321. Glucosamine6P_isomerase_CS.
[Graphical view]
PANTHERiPTHR11280. PTHR11280. 1 hit.
PfamiPF01182. Glucosamine_iso. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00502. nagB. 1 hit.
PROSITEiPS01161. GLC_GALNAC_ISOMERASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P46926-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLIILEHYS QASEWAAKYI RNRIIQFNPG PEKYFTLGLP TGSTPLGCYK
60 70 80 90 100
KLIEYYKNGD LSFKYVKTFN MDEYVGLPRD HPESYHSFMW NNFFKHIDIH
110 120 130 140 150
PENTHILDGN AVDLQAECDA FEEKIKAAGG IELFVGGIGP DGHIAFNEPG
160 170 180 190 200
SSLVSRTRVK TLAMDTILAN ARFFDGELTK VPTMALTVGV GTVMDAREVM
210 220 230 240 250
ILITGAHKAF ALYKAIEEGV NHMWTVSAFQ QHPRTVFVCD EDATLELKVK
260 270 280
TVKYFKGLML VHNKLVDPLY SIKEKETEKS QSSKKPYSD
Length:289
Mass (Da):32,669
Last modified:November 1, 1995 - v1
Checksum:i4111F655D574F74F
GO
Isoform 2 (identifier: P46926-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     69-145: Missing.

Note: No experimental confirmation available.
Show »
Length:212
Mass (Da):24,047
Checksum:i1E7290F5BF06DB34
GO

Sequence cautioni

The sequence BAA06544 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05701169 – 145Missing in isoform 2. 1 PublicationAdd BLAST77

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048826 mRNA. Translation: AAC05123.1.
AJ002231 mRNA. Translation: CAA05259.1.
AF029914 mRNA. Translation: AAB84217.1.
AF035809
, AF035804, AF035805, AF035806, AF035807, AF035808 Genomic DNA. Translation: AAB88748.1.
D31766 mRNA. Translation: BAA06544.2. Different initiation.
AK296452 mRNA. Translation: BAH12360.1.
AC005740 Genomic DNA. Translation: AAC62119.1.
CH471062 Genomic DNA. Translation: EAW61890.1.
CH471062 Genomic DNA. Translation: EAW61891.1.
CH471062 Genomic DNA. Translation: EAW61892.1.
CH471062 Genomic DNA. Translation: EAW61893.1.
BC012853 mRNA. Translation: AAH12853.1.
BC020769 mRNA. Translation: AAH20769.1.
BC022322 mRNA. Translation: AAH22322.1.
CCDSiCCDS4272.1. [P46926-1]
RefSeqiNP_005462.1. NM_005471.4. [P46926-1]
XP_006714810.1. XM_006714747.2. [P46926-1]
XP_011535839.1. XM_011537537.1. [P46926-1]
UniGeneiHs.633853.

Genome annotation databases

EnsembliENST00000311337; ENSP00000311876; ENSG00000113552. [P46926-1]
ENST00000500692; ENSP00000424275; ENSG00000113552. [P46926-1]
ENST00000503794; ENSP00000423485; ENSG00000113552. [P46926-1]
ENST00000508177; ENSP00000423674; ENSG00000113552. [P46926-1]
GeneIDi10007.
KEGGihsa:10007.
UCSCiuc003lmf.5. human. [P46926-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048826 mRNA. Translation: AAC05123.1.
AJ002231 mRNA. Translation: CAA05259.1.
AF029914 mRNA. Translation: AAB84217.1.
AF035809
, AF035804, AF035805, AF035806, AF035807, AF035808 Genomic DNA. Translation: AAB88748.1.
D31766 mRNA. Translation: BAA06544.2. Different initiation.
AK296452 mRNA. Translation: BAH12360.1.
AC005740 Genomic DNA. Translation: AAC62119.1.
CH471062 Genomic DNA. Translation: EAW61890.1.
CH471062 Genomic DNA. Translation: EAW61891.1.
CH471062 Genomic DNA. Translation: EAW61892.1.
CH471062 Genomic DNA. Translation: EAW61893.1.
BC012853 mRNA. Translation: AAH12853.1.
BC020769 mRNA. Translation: AAH20769.1.
BC022322 mRNA. Translation: AAH22322.1.
CCDSiCCDS4272.1. [P46926-1]
RefSeqiNP_005462.1. NM_005471.4. [P46926-1]
XP_006714810.1. XM_006714747.2. [P46926-1]
XP_011535839.1. XM_011537537.1. [P46926-1]
UniGeneiHs.633853.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NE7X-ray1.75A/B/C/D/E/F1-289[»]
ProteinModelPortaliP46926.
SMRiP46926.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115325. 37 interactors.
IntActiP46926. 4 interactors.
STRINGi9606.ENSP00000311876.

PTM databases

iPTMnetiP46926.
PhosphoSitePlusiP46926.

Polymorphism and mutation databases

BioMutaiGNPDA1.

Proteomic databases

EPDiP46926.
MaxQBiP46926.
PaxDbiP46926.
PeptideAtlasiP46926.
PRIDEiP46926.

Protocols and materials databases

DNASUi10007.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000311337; ENSP00000311876; ENSG00000113552. [P46926-1]
ENST00000500692; ENSP00000424275; ENSG00000113552. [P46926-1]
ENST00000503794; ENSP00000423485; ENSG00000113552. [P46926-1]
ENST00000508177; ENSP00000423674; ENSG00000113552. [P46926-1]
GeneIDi10007.
KEGGihsa:10007.
UCSCiuc003lmf.5. human. [P46926-1]

Organism-specific databases

CTDi10007.
DisGeNETi10007.
GeneCardsiGNPDA1.
HGNCiHGNC:4417. GNPDA1.
HPAiHPA000499.
HPA046891.
MIMi601798. gene.
neXtProtiNX_P46926.
OpenTargetsiENSG00000113552.
PharmGKBiPA28796.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3148. Eukaryota.
COG0363. LUCA.
GeneTreeiENSGT00390000014316.
HOGENOMiHOG000064979.
HOVERGENiHBG002546.
InParanoidiP46926.
KOiK02564.
PhylomeDBiP46926.
TreeFamiTF300841.

Enzyme and pathway databases

BioCyciZFISH:HS03692-MONOMER.
BRENDAi3.5.99.6. 2681.
ReactomeiR-HSA-70326. Glucose metabolism.
SABIO-RKP46926.

Miscellaneous databases

ChiTaRSiGNPDA1. human.
EvolutionaryTraceiP46926.
GeneWikiiGNPDA1.
GenomeRNAii10007.
PROiP46926.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000113552.
CleanExiHS_GNPDA1.
ExpressionAtlasiP46926. baseline and differential.
GenevisibleiP46926. HS.

Family and domain databases

CDDicd01399. GlcN6P_deaminase. 1 hit.
HAMAPiMF_01241. GlcN6P_deamin. 1 hit.
InterProiIPR006148. Glc/Gal-6P_isomerase.
IPR004547. Glucosamine6P_isomerase.
IPR018321. Glucosamine6P_isomerase_CS.
[Graphical view]
PANTHERiPTHR11280. PTHR11280. 1 hit.
PfamiPF01182. Glucosamine_iso. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00502. nagB. 1 hit.
PROSITEiPS01161. GLC_GALNAC_ISOMERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGNPI1_HUMAN
AccessioniPrimary (citable) accession number: P46926
Secondary accession number(s): B7Z3X4, D3DQE7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 162 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.