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P46926 (GNPI1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucosamine-6-phosphate isomerase 1

EC=3.5.99.6
Alternative name(s):
Glucosamine-6-phosphate deaminase 1
Short name=GNPDA 1
Short name=GlcN6P deaminase 1
Oscillin
Gene names
Name:GNPDA1
Synonyms:GNPI, HLN, KIAA0060
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length289 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Seems to trigger calcium oscillations in mammalian eggs. These oscillations serve as the essential trigger for egg activation and early development of the embryo By similarity. HAMAP-Rule MF_01241

Catalytic activity

Alpha-D-glucosamine 6-phosphate + H2O = D-fructose 6-phosphate + NH3. HAMAP-Rule MF_01241

Subunit structure

Homohexamer. Ref.10

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01241.

Sequence similarities

Belongs to the glucosamine/galactosamine-6-phosphate isomerase family.

Sequence caution

The sequence BAA06544.2 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 289289Glucosamine-6-phosphate isomerase 1 HAMAP-Rule MF_01241
PRO_0000160122

Sites

Active site721Proton acceptor; for enolization step By similarity
Active site1411For ring-opening step By similarity
Active site1431Proton acceptor; for ring-opening step By similarity
Active site1481For ring-opening step By similarity

Amino acid modifications

Modified residue641N6-acetyllysine Ref.8

Secondary structure

............................................... 289
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P46926 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 4111F655D574F74F

FASTA28932,669
        10         20         30         40         50         60 
MKLIILEHYS QASEWAAKYI RNRIIQFNPG PEKYFTLGLP TGSTPLGCYK KLIEYYKNGD 

        70         80         90        100        110        120 
LSFKYVKTFN MDEYVGLPRD HPESYHSFMW NNFFKHIDIH PENTHILDGN AVDLQAECDA 

       130        140        150        160        170        180 
FEEKIKAAGG IELFVGGIGP DGHIAFNEPG SSLVSRTRVK TLAMDTILAN ARFFDGELTK 

       190        200        210        220        230        240 
VPTMALTVGV GTVMDAREVM ILITGAHKAF ALYKAIEEGV NHMWTVSAFQ QHPRTVFVCD 

       250        260        270        280 
EDATLELKVK TVKYFKGLML VHNKLVDPLY SIKEKETEKS QSSKKPYSD 

« Hide

References

« Hide 'large scale' references
[1]"Molecularly cloned mammalian glucosamine-6-phosphate deaminase localizes to transporting epithelium and lacks oscillin activity."
Wolosker H., Kline D., Bian Y., Blackshaw S., Cameron A.M., Fralich T.J., Schnaar R.L., Snyder S.H.
FASEB J. 12:91-99(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"The human glucosamine-6-phosphate deaminase gene: cDNA cloning and expression, genomic organization and chromosomal localization."
Shevchenko V., Hogben M., Ekong R., Parrington J., Lai F.A.
Gene 216:31-38(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Testis.
[3]Hirata S., Koh T., Hoshi K.
Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Tissue: Testis.
[4]"Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1."
Nomura N., Nagase T., Miyajima N., Sazuka T., Tanaka A., Sato S., Seki N., Kawarabayasi Y., Ishikawa K., Tabata S.
DNA Res. 1:223-229(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Bone marrow.
[5]"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. expand/collapse author list , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary and Skin.
[8]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-64, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Two mammalian glucosamine-6-phosphate deaminases: a structural and genetic study."
Arreola R., Valderrama B., Morante M.L., Horjales E.
FEBS Lett. 551:63-70(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS), SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF048826 mRNA. Translation: AAC05123.1.
AJ002231 mRNA. Translation: CAA05259.1.
AF029914 mRNA. Translation: AAB84217.1.
AF035809 expand/collapse EMBL AC list , AF035804, AF035805, AF035806, AF035807, AF035808 Genomic DNA. Translation: AAB88748.1.
D31766 mRNA. Translation: BAA06544.2. Different initiation.
AC005740 Genomic DNA. Translation: AAC62119.1.
CH471062 Genomic DNA. Translation: EAW61890.1.
CH471062 Genomic DNA. Translation: EAW61891.1.
CH471062 Genomic DNA. Translation: EAW61892.1.
CH471062 Genomic DNA. Translation: EAW61893.1.
BC012853 mRNA. Translation: AAH12853.1.
BC020769 mRNA. Translation: AAH20769.1.
BC022322 mRNA. Translation: AAH22322.1.
CCDSCCDS4272.1.
RefSeqNP_005462.1. NM_005471.4.
XP_006714810.1. XM_006714747.1.
UniGeneHs.633853.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1NE7X-ray1.75A/B/C/D/E/F1-289[»]
ProteinModelPortalP46926.
SMRP46926. Positions 1-281.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115325. 23 interactions.
IntActP46926. 1 interaction.
STRING9606.ENSP00000311876.

PTM databases

PhosphoSiteP46926.

Proteomic databases

MaxQBP46926.
PaxDbP46926.
PeptideAtlasP46926.
PRIDEP46926.

Protocols and materials databases

DNASU10007.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000311337; ENSP00000311876; ENSG00000113552.
ENST00000500692; ENSP00000424275; ENSG00000113552.
ENST00000503794; ENSP00000423485; ENSG00000113552.
ENST00000508177; ENSP00000423674; ENSG00000113552.
GeneID10007.
KEGGhsa:10007.
UCSCuc003lmf.4. human.

Organism-specific databases

CTD10007.
GeneCardsGC05M141360.
HGNCHGNC:4417. GNPDA1.
HPAHPA000499.
HPA046891.
MIM601798. gene.
neXtProtNX_P46926.
PharmGKBPA28796.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0363.
HOGENOMHOG000064979.
HOVERGENHBG002546.
InParanoidP46926.
KOK02564.
PhylomeDBP46926.
TreeFamTF300841.

Enzyme and pathway databases

SABIO-RKP46926.

Gene expression databases

ArrayExpressP46926.
BgeeP46926.
CleanExHS_GNPDA1.
GenevestigatorP46926.

Family and domain databases

HAMAPMF_01241. GlcN6P_deamin.
InterProIPR006148. Glc/Gal-6P_isomerase.
IPR004547. Glucosamine6P_isomerase.
IPR018321. Glucosamine6P_isomerase_CS.
[Graphical view]
PANTHERPTHR11280. PTHR11280. 1 hit.
PfamPF01182. Glucosamine_iso. 1 hit.
[Graphical view]
TIGRFAMsTIGR00502. nagB. 1 hit.
PROSITEPS01161. GLC_GALNAC_ISOMERASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP46926.
GeneWikiGNPDA1.
GenomeRNAi10007.
NextBio37805.
PROP46926.
SOURCESearch...

Entry information

Entry nameGNPI1_HUMAN
AccessionPrimary (citable) accession number: P46926
Secondary accession number(s): D3DQE7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM