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Protein

Trimethylamine-N-oxide reductase 2

Gene

torZ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Reduces trimethylamine-N-oxide (TMAO) into trimethylamine; an anaerobic reaction coupled to energy-yielding reactions. Can also reduce other N- and S-oxide compounds such as 4-methylmorpholine-N-oxide and biotin sulfoxide (BSO), but with a lower catalytic efficiency.

Catalytic activityi

Trimethylamine + 2 (ferricytochrome c)-subunit + H2O = trimethylamine N-oxide + 2 (ferrocytochrome c)-subunit + 2 H+.

Cofactori

Mo-bis(molybdopterin guanine dinucleotide)By similarityNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi176 – 1761MolybdenumBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciEcoCyc:G7022-MONOMER.
ECOL316407:JW1861-MONOMER.
MetaCyc:G7022-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Trimethylamine-N-oxide reductase 2 (EC:1.7.2.3)
Short name:
TMAO reductase 2
Short name:
Trimethylamine oxidase 2
Gene namesi
Name:torZ
Synonyms:bisZ
Ordered Locus Names:b1872, JW1861
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13276. torZ.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Tat-type signalPROSITE-ProRule annotation1 PublicationAdd
BLAST
Chaini32 – 809778Trimethylamine-N-oxide reductase 2PRO_0000019163Add
BLAST

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven. Can also be exported by the Sec system.

Proteomic databases

PaxDbiP46923.
PRIDEiP46923.

Interactioni

Protein-protein interaction databases

BioGridi4263502. 9 interactions.
DIPiDIP-9226N.
IntActiP46923. 6 interactions.
STRINGi511145.b1872.

Structurei

3D structure databases

ProteinModelPortaliP46923.
SMRiP46923. Positions 36-805.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QY8. Bacteria.
COG0243. LUCA.
HOGENOMiHOG000284391.
InParanoidiP46923.
KOiK07812.
OMAiHGEQTHW.
PhylomeDBiP46923.

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR006658. BisC.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR00509. bisC_fam. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46923-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLTRREFIK HSGIAAGALV VTSAAPLPAW AEEKGGKILT AGRWGAMNVE
60 70 80 90 100
VKDGKIVSST GALAKTIPNS LQSTAADQVH TTARIQHPMV RKSYLDNPLQ
110 120 130 140 150
PAKGRGEDTY VQVSWEQALK LIHEQHDRIR KANGPSAIFA GSYGWRSSGV
160 170 180 190 200
LHKAQTLLQR YMNLAGGYSG HSGDYSTGAA QVIMPHVVGS VEVYEQQTSW
210 220 230 240 250
PLILENSQVV VLWGMNPLNT LKIAWSSTDE QGLEYFHQLK KSGKPVIAID
260 270 280 290 300
PIRSETIEFF DDNATWIAPN MGTDVALMLG IAHTLMTQGK HDKVFLEKYT
310 320 330 340 350
TGYPQFEEYL TGKSDNTPKS AVWAAEITGV PEAQIVKLAE LMAANRTMLM
360 370 380 390 400
AGWGIQRQQY GEQKHWMLVT LAAMLGQIGT PGGGFGFSYH YSNGGNPTRV
410 420 430 440 450
GGVLPEMSAA IAGHASEAAD DGGMTAIPVA RIVDALENPG GKYQHNGKEQ
460 470 480 490 500
TYPNIKMIWW AGGGNFTHHQ DTNRLIKAWQ KPEMIVVSEC YWTAAAKHAD
510 520 530 540 550
IVLPITTSFE RNDLTMTGDY SNQHIVPMKQ AVAPQFEARN DFDVFADLAE
560 570 580 590 600
LLKPGGKEIY TEGKDEMAWL KFFYDAAQKG ARAQRVTMPM FNAFWQQNKL
610 620 630 640 650
IEMRHSEKNE QYVRYGDFRA DPVKNALGTP SGKIEIYSKT LEKFGYKDCP
660 670 680 690 700
AHPTWLAPDE WKGTADEKQL QLLTAHPAHR LHSQLNYAEL RKKYAIADRE
710 720 730 740 750
PITIHTEDAA RFGIANGDLV RVWNKRGQIL TGAVVTDGIK KGVVCVHEGA
760 770 780 790 800
WPDLENGLCK NGSANVLTAD IPSSQLANAC AGNSALVYIE KYTGNAPKLT

AFDQPAVQA
Length:809
Mass (Da):88,964
Last modified:November 1, 1997 - v2
Checksum:i44A84F6302531D09
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti76 – 761A → T in AAC44131 (PubMed:8919859).Curated
Sequence conflicti291 – 2922HD → TI in AAC44131 (PubMed:8919859).Curated
Sequence conflicti299 – 3046YTTGYP → TLPGIR in AAC44131 (PubMed:8919859).Curated
Sequence conflicti406 – 4094EMSA → DFSGP in AAC44131 (PubMed:8919859).Curated
Sequence conflicti801 – 8033AFD → GFG in AAC44131 (PubMed:8919859).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38839 Genomic DNA. Translation: AAC44131.1.
U00096 Genomic DNA. Translation: AAC74942.2.
AP009048 Genomic DNA. Translation: BAA15682.1.
PIRiH64949.
RefSeqiNP_416386.4. NC_000913.3.
WP_000176781.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74942; AAC74942; b1872.
BAA15682; BAA15682; BAA15682.
GeneIDi946389.
KEGGiecj:JW1861.
eco:b1872.
PATRICi32119069. VBIEscCol129921_1952.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38839 Genomic DNA. Translation: AAC44131.1.
U00096 Genomic DNA. Translation: AAC74942.2.
AP009048 Genomic DNA. Translation: BAA15682.1.
PIRiH64949.
RefSeqiNP_416386.4. NC_000913.3.
WP_000176781.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP46923.
SMRiP46923. Positions 36-805.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263502. 9 interactions.
DIPiDIP-9226N.
IntActiP46923. 6 interactions.
STRINGi511145.b1872.

Proteomic databases

PaxDbiP46923.
PRIDEiP46923.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74942; AAC74942; b1872.
BAA15682; BAA15682; BAA15682.
GeneIDi946389.
KEGGiecj:JW1861.
eco:b1872.
PATRICi32119069. VBIEscCol129921_1952.

Organism-specific databases

EchoBASEiEB3061.
EcoGeneiEG13276. torZ.

Phylogenomic databases

eggNOGiENOG4107QY8. Bacteria.
COG0243. LUCA.
HOGENOMiHOG000284391.
InParanoidiP46923.
KOiK07812.
OMAiHGEQTHW.
PhylomeDBiP46923.

Enzyme and pathway databases

BioCyciEcoCyc:G7022-MONOMER.
ECOL316407:JW1861-MONOMER.
MetaCyc:G7022-MONOMER.

Miscellaneous databases

PROiP46923.

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR006658. BisC.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR00509. bisC_fam. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTORZ_ECOLI
AccessioniPrimary (citable) accession number: P46923
Secondary accession number(s): P76292, P97187
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Expression of torYZ allows E.coli to grow anaerobically on a wider range of substrates than does expression of torCAD.

Caution

Was originally thought to be a biotin sulfoxide reductase.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.