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Protein

Trimethylamine-N-oxide reductase 2

Gene

torZ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reduces trimethylamine-N-oxide (TMAO) into trimethylamine; an anaerobic reaction coupled to energy-yielding reactions. Can also reduce other N- and S-oxide compounds such as 4-methylmorpholine-N-oxide and biotin sulfoxide (BSO), but with a lower catalytic efficiency.

Miscellaneous

Expression of torYZ allows E.coli to grow anaerobically on a wider range of substrates than does expression of torCAD.

Caution

Was originally thought to be a biotin sulfoxide reductase.1 Publication

Catalytic activityi

Trimethylamine + 2 (ferricytochrome c)-subunit + H2O = trimethylamine N-oxide + 2 (ferrocytochrome c)-subunit + 2 H+.

Cofactori

Mo-bis(molybdopterin guanine dinucleotide)By similarityNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi176MolybdenumBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, Molybdenum

Enzyme and pathway databases

BioCyciEcoCyc:G7022-MONOMER
MetaCyc:G7022-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Trimethylamine-N-oxide reductase 2 (EC:1.7.2.3)
Short name:
TMAO reductase 2
Short name:
Trimethylamine oxidase 2
Gene namesi
Name:torZ
Synonyms:bisZ
Ordered Locus Names:b1872, JW1861
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13276 torZ

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Tat-type signalPROSITE-ProRule annotation1 PublicationAdd BLAST31
ChainiPRO_000001916332 – 809Trimethylamine-N-oxide reductase 2Add BLAST778

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven. Can also be exported by the Sec system.

Proteomic databases

PaxDbiP46923
PRIDEiP46923

Interactioni

Protein-protein interaction databases

BioGridi4263502, 53 interactors
DIPiDIP-9226N
IntActiP46923, 19 interactors
STRINGi316385.ECDH10B_2013

Structurei

3D structure databases

ProteinModelPortaliP46923
SMRiP46923
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QY8 Bacteria
COG0243 LUCA
HOGENOMiHOG000284391
InParanoidiP46923
KOiK07812
OMAiKGDYSTG
PhylomeDBiP46923

Family and domain databases

InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006658 BisC
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006655 Mopterin_OxRdtase_prok_CS
IPR006311 TAT_signal
IPR019546 TAT_signal_bac_arc
PfamiView protein in Pfam
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR00509 bisC_fam, 1 hit
TIGR01409 TAT_signal_seq, 1 hit
PROSITEiView protein in PROSITE
PS00490 MOLYBDOPTERIN_PROK_2, 1 hit
PS00932 MOLYBDOPTERIN_PROK_3, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46923-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLTRREFIK HSGIAAGALV VTSAAPLPAW AEEKGGKILT AGRWGAMNVE
60 70 80 90 100
VKDGKIVSST GALAKTIPNS LQSTAADQVH TTARIQHPMV RKSYLDNPLQ
110 120 130 140 150
PAKGRGEDTY VQVSWEQALK LIHEQHDRIR KANGPSAIFA GSYGWRSSGV
160 170 180 190 200
LHKAQTLLQR YMNLAGGYSG HSGDYSTGAA QVIMPHVVGS VEVYEQQTSW
210 220 230 240 250
PLILENSQVV VLWGMNPLNT LKIAWSSTDE QGLEYFHQLK KSGKPVIAID
260 270 280 290 300
PIRSETIEFF DDNATWIAPN MGTDVALMLG IAHTLMTQGK HDKVFLEKYT
310 320 330 340 350
TGYPQFEEYL TGKSDNTPKS AVWAAEITGV PEAQIVKLAE LMAANRTMLM
360 370 380 390 400
AGWGIQRQQY GEQKHWMLVT LAAMLGQIGT PGGGFGFSYH YSNGGNPTRV
410 420 430 440 450
GGVLPEMSAA IAGHASEAAD DGGMTAIPVA RIVDALENPG GKYQHNGKEQ
460 470 480 490 500
TYPNIKMIWW AGGGNFTHHQ DTNRLIKAWQ KPEMIVVSEC YWTAAAKHAD
510 520 530 540 550
IVLPITTSFE RNDLTMTGDY SNQHIVPMKQ AVAPQFEARN DFDVFADLAE
560 570 580 590 600
LLKPGGKEIY TEGKDEMAWL KFFYDAAQKG ARAQRVTMPM FNAFWQQNKL
610 620 630 640 650
IEMRHSEKNE QYVRYGDFRA DPVKNALGTP SGKIEIYSKT LEKFGYKDCP
660 670 680 690 700
AHPTWLAPDE WKGTADEKQL QLLTAHPAHR LHSQLNYAEL RKKYAIADRE
710 720 730 740 750
PITIHTEDAA RFGIANGDLV RVWNKRGQIL TGAVVTDGIK KGVVCVHEGA
760 770 780 790 800
WPDLENGLCK NGSANVLTAD IPSSQLANAC AGNSALVYIE KYTGNAPKLT

AFDQPAVQA
Length:809
Mass (Da):88,964
Last modified:November 1, 1997 - v2
Checksum:i44A84F6302531D09
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti76A → T in AAC44131 (PubMed:8919859).Curated1
Sequence conflicti291 – 292HD → TI in AAC44131 (PubMed:8919859).Curated2
Sequence conflicti299 – 304YTTGYP → TLPGIR in AAC44131 (PubMed:8919859).Curated6
Sequence conflicti406 – 409EMSA → DFSGP in AAC44131 (PubMed:8919859).Curated4
Sequence conflicti801 – 803AFD → GFG in AAC44131 (PubMed:8919859).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38839 Genomic DNA Translation: AAC44131.1
U00096 Genomic DNA Translation: AAC74942.2
AP009048 Genomic DNA Translation: BAA15682.1
PIRiH64949
RefSeqiNP_416386.4, NC_000913.3
WP_000176781.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74942; AAC74942; b1872
BAA15682; BAA15682; BAA15682
GeneIDi946389
KEGGiecj:JW1861
eco:b1872
PATRICifig|1411691.4.peg.376

Similar proteinsi

Entry informationi

Entry nameiTORZ_ECOLI
AccessioniPrimary (citable) accession number: P46923
Secondary accession number(s): P76292, P97187
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health