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Protein

Arginine--tRNA ligase

Gene

argS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).

GO - Molecular functioni

  1. arginine-tRNA ligase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU37330-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine--tRNA ligase (EC:6.1.1.19)
Alternative name(s):
Arginyl-tRNA synthetase
Short name:
ArgRS
Gene namesi
Name:argS
Ordered Locus Names:BSU37330
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU37330. [Micado]

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Arginine--tRNA ligasePRO_0000151530Add
BLAST

Proteomic databases

PaxDbiP46906.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiP46906. 1 interaction.
MINTiMINT-8366397.
STRINGi224308.BSU37330.

Structurei

3D structure databases

ProteinModelPortaliP46906.
SMRiP46906. Positions 1-556.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi132 – 14211"HIGH" regionAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0018.
HOGENOMiHOG000247214.
InParanoidiP46906.
KOiK01887.
OMAiLDTHLEF.
OrthoDBiEOG6JB13C.
PhylomeDBiP46906.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00123. Arg_tRNA_synth.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR11956. PTHR11956. 1 hit.
PfamiPF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]
PRINTSiPR01038. TRNASYNTHARG.
SMARTiSM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsiTIGR00456. argS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46906-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIAEQMKDV LKEEIKAAVL KAGLAEESQI PNVVLETPKD KTHGDYSTNM
60 70 80 90 100
AMQLARVAKK APRQIAEEIV AHFDKGKASI EKLDIAGPGF INFYMNNQYL
110 120 130 140 150
TKLIPSVLEA GEAYGETNIG NGERVQVEFV SANPTGDLHL GHARGAAVGD
160 170 180 190 200
SLCNVLSKAG YDVSREYYIN DAGNQINNLA LSVEVRYFEA LGLEKPMPED
210 220 230 240 250
GYRGEDIIAI GKRLAEEYGD RFVNEEESER LAFFREYGLK YELEKLRKDL
260 270 280 290 300
ENFRVPFDVW YSETSLYQNG KIDKALEALR EKGHVYEEDG ATWFRSTTFG
310 320 330 340 350
DDKDRVLIKK DGTYTYLLPD IAYHKDKLDR GFDKLINVWG ADHHGYIPRM
360 370 380 390 400
KAAIEALGYE KGTLEVEIIQ LVHLYKNGEK MKMSKRTGKA VTMRDLIEEV
410 420 430 440 450
GLDAVRYFFA MRSADTHMDF DLDLAVSTSN ENPVYYAQYA HARICSMLRQ
460 470 480 490 500
GEEQGLKPAA DLDFSHIQSE KEYDLLKTIG GFPEAVAEAA EKRIPHRVTN
510 520 530 540 550
YIYDLASALH SFYNAEKVID PENEEKSRAR LALMKATQIT LNNALQLIGV

SAPEKM
Length:556
Mass (Da):62,723
Last modified:July 15, 1998 - v2
Checksum:i0D92ED44E50BCD7F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97024 Genomic DNA. Translation: CAB09703.1.
AL009126 Genomic DNA. Translation: CAB15761.1.
Z49884 Genomic DNA. Translation: CAA90040.1.
PIRiE69589.
RefSeqiNP_391614.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB15761; CAB15761; BSU37330.
GeneIDi937054.
KEGGibsu:BSU37330.
PATRICi18979504. VBIBacSub10457_3914.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97024 Genomic DNA. Translation: CAB09703.1.
AL009126 Genomic DNA. Translation: CAB15761.1.
Z49884 Genomic DNA. Translation: CAA90040.1.
PIRiE69589.
RefSeqiNP_391614.1. NC_000964.3.

3D structure databases

ProteinModelPortaliP46906.
SMRiP46906. Positions 1-556.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP46906. 1 interaction.
MINTiMINT-8366397.
STRINGi224308.BSU37330.

Proteomic databases

PaxDbiP46906.

Protocols and materials databases

DNASUi937054.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15761; CAB15761; BSU37330.
GeneIDi937054.
KEGGibsu:BSU37330.
PATRICi18979504. VBIBacSub10457_3914.

Organism-specific databases

GenoListiBSU37330. [Micado]

Phylogenomic databases

eggNOGiCOG0018.
HOGENOMiHOG000247214.
InParanoidiP46906.
KOiK01887.
OMAiLDTHLEF.
OrthoDBiEOG6JB13C.
PhylomeDBiP46906.

Enzyme and pathway databases

BioCyciBSUB:BSU37330-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00123. Arg_tRNA_synth.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR11956. PTHR11956. 1 hit.
PfamiPF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]
PRINTSiPR01038. TRNASYNTHARG.
SMARTiSM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsiTIGR00456. argS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
    Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
    Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms."
    Cruz Ramos H., Boursier L., Moszer I., Kunst F., Danchin A., Glaser P.
    EMBO J. 14:5984-5994(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 442-556.
    Strain: 168.

Entry informationi

Entry nameiSYR_BACSU
AccessioniPrimary (citable) accession number: P46906
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 15, 1998
Last modified: January 7, 2015
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.