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P46891 (COF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
HMP-PP phosphatase

EC=3.6.1.-
Gene names
Name:cof
Ordered Locus Names:b0446, JW0436
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length272 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the hydrolysis of 4-amino-2-methyl-5-hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2-methyl-5-hydroxymethylpyrimidine phosphate (HMP-P). Can also hydrolyze other substrates such as MeO-HMP-PP and CF(3)-HMP-PP. Ref.6

Sequence similarities

Belongs to the HAD-like hydrolase superfamily. Cof family.

Sequence caution

The sequence AAB40202.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence CAA91181.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processthiamine biosynthetic process

Inferred from mutant phenotype Ref.6. Source: EcoliWiki

   Molecular functionnucleoside-diphosphatase activity

Inferred from direct assay Ref.6. Source: EcoliWiki

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

groLP0A6F51EBI-1117256,EBI-543750

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 272272HMP-PP phosphatase HAMAP MF_01847
PRO_0000054418

Experimental info

Sequence conflict1731C → S in BAA11650. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P46891 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: D8FC034BF81E41AC

FASTA27230,371
        10         20         30         40         50         60 
MARLAAFDMD GTLLMPDHHL GEKTLSTLAR LRERDITLTF ATGRHALEMQ HILGALSLDA 

        70         80         90        100        110        120 
YLITGNGTRV HSLEGELLHR DDLPADVAEL VLYQQWDTRA SMHIFNDDGW FTGKEIPALL 

       130        140        150        160        170        180 
QAFVYSGFRY QIIDVKKMPL GSVTKICFCG DHDDLTRLQI QLYEALGERA HLCFSATDCL 

       190        200        210        220        230        240 
EVLPVGCNKG AALTVLTQHL GLSLRDCMAF GDAMNDREML VSVGSGFIMG NAMPQLRAEL 

       250        260        270 
PHLPVIGHCR NQAVSHYLTH WLDYPHLPYS PE 

« Hide

References

« Hide 'large scale' references
[1]Patzer S.I., Hantke K.
Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[2]"Nucleotide sequence analysis of the E. coli chromosome around the 10.0 min region."
Hatada E., Ohmori H., Qiao Y., Tsuji M., Fukuda R.
Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"A genetic screen for the identification of thiamin metabolic genes."
Lawhorn B.G., Gerdes S.Y., Begley T.P.
J. Biol. Chem. 279:43555-43559(2004) [PubMed: 15292217] [Abstract]
Cited for: FUNCTION.
Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z54355 Genomic DNA. Translation: CAA91181.1. Different initiation.
D82943 Genomic DNA. Translation: BAA11650.1.
U82664 Genomic DNA. Translation: AAB40202.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73549.2.
AP009048 Genomic DNA. Translation: BAE76226.1.
PIRF64774.
RefSeqNP_414980.2. NC_000913.2.

3D structure databases

ProteinModelPortalP46891.
SMRP46891. Positions 2-262.
ModBaseSearch...

Protein-protein interaction databases

IntActP46891. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000002338; EBESCP00000002338; EBESCG00000001908.
EBESCT00000017641; EBESCP00000016932; EBESCG00000016697.
GeneID945089.
GenomeReviewsGene locus JW0436 in contig AP009048_GR.
Gene locus b0446 in contig U00096_GR.
KEGGecj:JW0436.
eco:b0446.
PATRIC32116045. VBIEscCol129921_0464.

Organism-specific databases

EchoBASEEB3007.
EcoGeneEG13216. cof.

Phylogenomic databases

eggNOGCOG0561.
GeneTreeEBGT00050000009623.
HOGENOMHBG728500.
OMALDAFLIT.
PhylomeDBP46891.
ProtClustDBPRK15126.

Enzyme and pathway databases

BioCycEcoCyc:G6246-MONOMER.
MetaCyc:G6246-MONOMER.

Gene expression databases

GenevestigatorP46891.

Family and domain databases

HAMAPMF_01847. HMP-PP_phosphat.
[Tree]
InterProIPR005834. Dehalogen-like_hydro.
IPR023214. HAD-like_dom.
IPR006379. HAD-SF_hydro_IIB.
IPR023938. HMP-PP_phosphatase.
IPR000150. Hypothet_cof.
[Graphical view]
Gene3DG3DSA:3.40.50.1000. HAD-like_dom. 2 hits.
KOK11938.
PfamPF00702. Hydrolase. 1 hit.
[Graphical view]
SUPFAMSSF56784. HAD-like_dom. 1 hit.
TIGRFAMsTIGR00099. Cof-subfamily. 1 hit.
TIGR01484. HAD-SF-IIB. 1 hit.
PROSITEPS01228. COF_1. 1 hit.
PS01229. COF_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCOF_ECOLI
AccessionPrimary (citable) accession number: P46891
Secondary accession number(s): P71208, P77198, Q2MBY0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: January 25, 2012
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families