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Protein

Thermoresistant gluconokinase

Gene

gntK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + D-gluconate = ADP + 6-phospho-D-gluconate.

Pathwayi: D-gluconate degradation

This protein is involved in the pathway D-gluconate degradation, which is part of Carbohydrate acid metabolism.
View all proteins of this organism that are known to be involved in the pathway D-gluconate degradation and in Carbohydrate acid metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 22ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • gluconokinase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:GLUCONOKINII-MONOMER.
ECOL316407:JW3400-MONOMER.
MetaCyc:GLUCONOKINII-MONOMER.
UniPathwayiUPA00792.

Names & Taxonomyi

Protein namesi
Recommended name:
Thermoresistant gluconokinase (EC:2.7.1.12)
Alternative name(s):
Gluconate kinase 2
Gene namesi
Name:gntK
Ordered Locus Names:b3437, JW3400
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12629. gntK.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000875372 – 175Thermoresistant gluconokinaseAdd BLAST174

Proteomic databases

PaxDbiP46859.
PRIDEiP46859.

Interactioni

Protein-protein interaction databases

DIPiDIP-9820N.
IntActiP46859. 2 interactors.
STRINGi511145.b3437.

Structurei

Secondary structure

1175
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 14Combined sources7
Helixi21 – 32Combined sources12
Beta strandi35 – 38Combined sources4
Helixi39 – 42Combined sources4
Helixi45 – 52Combined sources8
Helixi59 – 79Combined sources21
Beta strandi81 – 86Combined sources6
Helixi92 – 99Combined sources8
Beta strandi105 – 111Combined sources7
Helixi114 – 122Combined sources9
Helixi131 – 140Combined sources10
Beta strandi151 – 155Combined sources5
Helixi160 – 172Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KNQX-ray2.00A/B1-175[»]
1KO1X-ray2.09A/B1-175[»]
1KO4X-ray2.50A/B1-175[»]
1KO5X-ray2.28A/B1-175[»]
1KO8X-ray2.40A/B1-175[»]
1KOFX-ray2.80A/B1-175[»]
ProteinModelPortaliP46859.
SMRiP46859.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP46859.

Family & Domainsi

Sequence similaritiesi

Belongs to the gluconokinase GntK/GntV family.Curated

Phylogenomic databases

eggNOGiENOG4108Z67. Bacteria.
COG3265. LUCA.
HOGENOMiHOG000032567.
InParanoidiP46859.
KOiK00851.
OMAiVPYAEGD.
PhylomeDBiP46859.

Family and domain databases

CDDicd02021. GntK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR031322. Shikimate/glucono_kinase.
IPR006001. Therm_gnt_kin.
[Graphical view]
PfamiPF01202. SKI. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01313. therm_gnt_kin. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46859-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTNHDHHI YVLMGVSGSG KSAVASEVAH QLHAAFLDGD FLHPRRNIEK
60 70 80 90 100
MASGEPLNDD DRKPWLQALN DAAFAMQRTN KVSLIVCSAL KKHYRDLLRE
110 120 130 140 150
GNPNLSFIYL KGDFDVIESR LKARKGHFFK TQMLVTQFET LQEPGADETD
160 170
VLVVDIDQPL EGVVASTIEV IKKGK
Length:175
Mass (Da):19,543
Last modified:January 23, 2007 - v4
Checksum:i5859A38413E8A586
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84362 Genomic DNA. Translation: BAA12325.1.
U18997 Genomic DNA. Translation: AAA58235.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76462.2.
AP009048 Genomic DNA. Translation: BAE77856.1.
RefSeqiNP_417894.2. NC_000913.3.
WP_000108330.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76462; AAC76462; b3437.
BAE77856; BAE77856; BAE77856.
GeneIDi947937.
KEGGiecj:JW3400.
eco:b3437.
PATRICi32122312. VBIEscCol129921_3533.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84362 Genomic DNA. Translation: BAA12325.1.
U18997 Genomic DNA. Translation: AAA58235.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76462.2.
AP009048 Genomic DNA. Translation: BAE77856.1.
RefSeqiNP_417894.2. NC_000913.3.
WP_000108330.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KNQX-ray2.00A/B1-175[»]
1KO1X-ray2.09A/B1-175[»]
1KO4X-ray2.50A/B1-175[»]
1KO5X-ray2.28A/B1-175[»]
1KO8X-ray2.40A/B1-175[»]
1KOFX-ray2.80A/B1-175[»]
ProteinModelPortaliP46859.
SMRiP46859.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-9820N.
IntActiP46859. 2 interactors.
STRINGi511145.b3437.

Proteomic databases

PaxDbiP46859.
PRIDEiP46859.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76462; AAC76462; b3437.
BAE77856; BAE77856; BAE77856.
GeneIDi947937.
KEGGiecj:JW3400.
eco:b3437.
PATRICi32122312. VBIEscCol129921_3533.

Organism-specific databases

EchoBASEiEB2513.
EcoGeneiEG12629. gntK.

Phylogenomic databases

eggNOGiENOG4108Z67. Bacteria.
COG3265. LUCA.
HOGENOMiHOG000032567.
InParanoidiP46859.
KOiK00851.
OMAiVPYAEGD.
PhylomeDBiP46859.

Enzyme and pathway databases

UniPathwayiUPA00792.
BioCyciEcoCyc:GLUCONOKINII-MONOMER.
ECOL316407:JW3400-MONOMER.
MetaCyc:GLUCONOKINII-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP46859.
PROiP46859.

Family and domain databases

CDDicd02021. GntK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR031322. Shikimate/glucono_kinase.
IPR006001. Therm_gnt_kin.
[Graphical view]
PfamiPF01202. SKI. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01313. therm_gnt_kin. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGNTK_ECOLI
AccessioniPrimary (citable) accession number: P46859
Secondary accession number(s): P78116, Q2M7A0, Q59404
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 125 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.