P46859 (GNTK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thermoresistant gluconokinase
EC=2.7.1.12

Alternative name(s):
Gluconate kinase 2

Gene names

Name:	gntK
Ordered Locus Names:	b3437, JW3400

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + D-gluconate = ADP + 6-phospho-D-gluconate.
Pathway	Carbohydrate acid metabolism; D-gluconate degradation.
Sequence similarities	Belongs to the gluconokinase GntK/GntV family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	D-gluconate catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW gluconokinase activity Inferred from direct assay PubMed 8925917. Source: EcoliWiki shikimate kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.4

Chain

2 – 175

174

Thermoresistant gluconokinase

PRO_0000087537

Regions

Nucleotide binding

15 – 22

8

ATP Potential

Secondary structure

1

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175

Helix Strand Turn

Details...

Beta strand

8 – 14

7

Helix

21 – 32

12

Beta strand

35 – 38

4

Helix

39 – 42

4

Helix

45 – 52

8

Helix

59 – 79

21

Beta strand

81 – 86

6

Helix

92 – 99

8

Beta strand

105 – 111

7

Helix

114 – 122

9

Helix

131 – 140

10

Beta strand

151 – 155

5

Helix

160 – 172

13

Sequences

	Sequence		Length	Mass (Da)
	P46859 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 5859A38413E8A586	FASTA	175	19,543
	10 20 30 40 50 60 MSTTNHDHHI YVLMGVSGSG KSAVASEVAH QLHAAFLDGD FLHPRRNIEK MASGEPLNDD 70 80 90 100 110 120 DRKPWLQALN DAAFAMQRTN KVSLIVCSAL KKHYRDLLRE GNPNLSFIYL KGDFDVIESR 130 140 150 160 170 LKARKGHFFK TQMLVTQFET LQEPGADETD VLVVDIDQPL EGVVASTIEV IKKGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene organization and transcriptional regulation of the gntRKU operon involved in gluconate uptake and catabolism of Escherichia coli." Izu H., Adachi O., Yamada M. J. Mol. Biol. 267:778-793(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Cloning and molecular genetic characterization of the Escherichia coli gntR, gntK, and gntU genes of GntI, the main system for gluconate metabolism." Tong S., Porco A., Isturiz T., Conway T. J. Bacteriol. 178:3260-3269(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-20, CHARACTERIZATION.
[5]	"Crystallization and preliminary X-ray crystallographic studies of recombinant thermoresistant gluconate kinase GntK from Escherichia coli." Kraft L., Sprenger G.A., Lindqvist Y. Acta Crystallogr. D 57:1159-1161(2001) [PubMed] [Europe PMC] [Abstract] Cited for: CRYSTALLIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D84362 Genomic DNA. Translation: BAA12325.1.
U18997 Genomic DNA. Translation: AAA58235.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76462.2.
AP009048 Genomic DNA. Translation: BAE77856.1.

RefSeq

NP_417894.2. NC_000913.2.
YP_491997.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KNQ	X-ray	2.00	A/B	1-175	[»]
1KO1	X-ray	2.09	A/B	1-175	[»]
1KO4	X-ray	2.50	A/B	1-175	[»]
1KO5	X-ray	2.28	A/B	1-175	[»]
1KO8	X-ray	2.40	A/B	1-175	[»]
1KOF	X-ray	2.80	A/B	1-175	[»]

ProteinModelPortal

P46859.

SMR

P46859. Positions 3-173.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-9820N.

IntAct

P46859. 2 interactions.

STRING

511145.b3437.

Proteomic databases

PRIDE

P46859.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC76462; AAC76462; b3437.
BAE77856; BAE77856; BAE77856.

GeneID

12932507.
947937.

KEGG

ecj:Y75_p3741.
eco:b3437.

PATRIC

32122312. VBIEscCol129921_3533.

Organism-specific databases

EchoBASE

EB2513.

EcoGene

EG12629. gntK.

Phylogenomic databases

eggNOG

COG3265.

HOGENOM

HOG000032567.

KO

K00851.

OMA

LRDGNPN.

ProtClustDB

PRK11545.

Enzyme and pathway databases

BioCyc

EcoCyc:GLUCONOKINII-MONOMER.
ECOL316407:JW3400-MONOMER.
MetaCyc:GLUCONOKINII-MONOMER.

UniPathway

UPA00792.

Gene expression databases

Genevestigator

P46859.

Family and domain databases

InterPro

IPR000623. Shikimate_kinase.
IPR006001. Therm_gnt_kin.
[Graphical view]

Pfam

PF01202. SKI. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01313. therm_gnt_kin. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P46859.

Entry information

Entry name

GNTK_ECOLI

Accession

Primary (citable) accession number: P46859
Secondary accession number(s): P78116, Q2M7A0, Q59404

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 105 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program