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Protein

L-amino acid N-acetyltransferase AaaT

Gene

aaaT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the N-acetylation of L-phenylalanine and L-methionine using acetyl-CoA as acetyl donor in vitro. Cannot accept L-tyrosine as substrate and propionyl-CoA, succinyl-CoA or (S)-methylmalonyl-CoA as acyl donors (PubMed:27941785). Is also able to acetylate and thus detoxify several nonhydrolyzable aminoacyl adenylates, but not the processed form of the peptide-nucleotide antibiotic microcin C (McC). When overproduced, provides complete resistance to leucyl sulfamoyl adenylate (LSA) and partial resistance to alanyl sulfamoyl adenylate (ASA) and phenylalanyl sulfamoyl adenylate (FSA). Therefore, may protect bacteria from various toxic aminoacyl nucleotides, either exogenous or those generated inside the cell during normal metabolism (PubMed:25002546).2 Publications

Catalytic activityi

Acetyl-CoA + L-phenylalanine = CoA + N-acetyl-L-phenylalanine.1 Publication
Acetyl-CoA + L-methionine = CoA + N-acetyl-L-methionine.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:G7758-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
L-amino acid N-acetyltransferase AaaT1 Publication
Alternative name(s):
L-methionine N-acetyltransferase1 Publication (EC:2.3.1.-1 Publication)
L-phenylalanine N-acetyltransferase1 Publication (EC:2.3.1.531 Publication)
Gene namesi
Name:aaaT1 Publication
Synonyms:yhhYImported
Ordered Locus Names:b3441, JW3405
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12945. yhhY.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000746131 – 162L-amino acid N-acetyltransferase AaaTAdd BLAST162

Proteomic databases

PaxDbiP46854.
PRIDEiP46854.

Interactioni

Protein-protein interaction databases

BioGridi4259623. 13 interactors.
IntActiP46854. 4 interactors.
STRINGi316385.ECDH10B_3615.

Structurei

3D structure databases

ProteinModelPortaliP46854.
SMRiP46854.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 162N-acetyltransferasePROSITE-ProRule annotationAdd BLAST159

Sequence similaritiesi

Belongs to the acetyltransferase family.Curated

Phylogenomic databases

eggNOGiENOG4105WCN. Bacteria.
COG0454. LUCA.
HOGENOMiHOG000078525.
InParanoidiP46854.
KOiK03825.
PhylomeDBiP46854.

Family and domain databases

InterProiView protein in InterPro
IPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
PfamiView protein in Pfam
PF00583. Acetyltransf_1. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiView protein in PROSITE
PS51186. GNAT. 1 hit.

Sequencei

Sequence statusi: Complete.

P46854-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEIVIRHAE TRDYEAIRQI HAQPEVYCNT LQVPHPSDHM WQERLADRPG
60 70 80 90 100
IKQLVACIDG DVVGHLTIDV QQRPRRSHVA DFGICVDSRW KNRGVASALM
110 120 130 140 150
REMIEMCDNW LRVDRIELTV FVDNAPAIKV YKKYGFEIEG TGKKYALRNG
160
EYVDAYYMAR VK
Length:162
Mass (Da):18,794
Last modified:November 1, 1995 - v1
Checksum:iF7565FA6906418E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58239.1.
U00096 Genomic DNA. Translation: AAC76466.1.
AP009048 Genomic DNA. Translation: BAE77852.1.
PIRiD65140.
RefSeqiNP_417898.1. NC_000913.3.
WP_001295206.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76466; AAC76466; b3441.
BAE77852; BAE77852; BAE77852.
GeneIDi947943.
KEGGiecj:JW3405.
eco:b3441.
PATRICifig|511145.12.peg.3538.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiAAAT_ECOLI
AccessioniPrimary (citable) accession number: P46854
Secondary accession number(s): Q2M7A4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 5, 2017
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families