Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Quercetin 2,3-dioxygenase

Gene

yhhW

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has quercetin 2,3-dioxygenase activity in vitro. Its physiological role is unknown; however, may provide a mechanism that would avoid inhibition of key cellular proteins, such as DNA gyrase, by quercetin.1 Publication

Miscellaneous

Quercetin is a flavonoid compound synthesized by a variety of plants, including foods for human consumption.

Catalytic activityi

Quercetin + O2 = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H+.1 Publication

Cofactori

Zn2+1 Publication, Co2+1 Publication, Fe2+1 PublicationNote: Binds 1 divalent metal cation, Zn2+, Co2+ or Fe2+.1 Publication

Enzyme regulationi

Inhibited by kojic acid, sodium diethyldithiocarbamate and 1,10-phenanthroline monohydrochloride.1 Publication

Pathwayi: quercetin degradation

This protein is involved in the pathway quercetin degradation, which is part of Flavonoid metabolism.
View all proteins of this organism that are known to be involved in the pathway quercetin degradation and in Flavonoid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi57Divalent metal cation1
Metal bindingi59Divalent metal cation1
Metal bindingi101Divalent metal cation1
Metal bindingi103Divalent metal cationBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • quercetin 2,3-dioxygenase activity Source: EcoCyc

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
LigandCopper, Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G7756-MONOMER
MetaCyc:G7756-MONOMER
UniPathwayiUPA00724

Names & Taxonomyi

Protein namesi
Recommended name:
Quercetin 2,3-dioxygenase (EC:1.13.11.24)
Short name:
Quercetinase
Alternative name(s):
Pirin-like protein YhhW
Gene namesi
Name:yhhW
Ordered Locus Names:b3439, JW3402
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12943 yhhW

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002140631 – 231Quercetin 2,3-dioxygenaseAdd BLAST231

Proteomic databases

PaxDbiP46852
PRIDEiP46852

Interactioni

Protein-protein interaction databases

BioGridi4261175, 19 interactors
DIPiDIP-12356N
IntActiP46852, 9 interactors
STRINGi316385.ECDH10B_3612

Structurei

Secondary structure

1231
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Helixi7 – 9Combined sources3
Beta strandi10 – 14Combined sources5
Beta strandi16 – 24Combined sources9
Beta strandi40 – 48Combined sources9
Beta strandi53 – 59Combined sources7
Beta strandi63 – 80Combined sources18
Beta strandi82 – 86Combined sources5
Beta strandi90 – 94Combined sources5
Beta strandi99 – 104Combined sources6
Beta strandi112 – 119Combined sources8
Beta strandi129 – 133Combined sources5
Beta strandi139 – 148Combined sources10
Helixi150 – 152Combined sources3
Beta strandi157 – 159Combined sources3
Beta strandi161 – 167Combined sources7
Beta strandi172 – 175Combined sources4
Beta strandi182 – 194Combined sources13
Beta strandi197 – 200Combined sources4
Beta strandi204 – 209Combined sources6
Beta strandi213 – 228Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TQ5X-ray1.76A1-231[»]
ProteinModelPortaliP46852
SMRiP46852
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP46852

Family & Domainsi

Domaini

Is composed of two structurally similar domains arranged face to face.1 Publication

Sequence similaritiesi

Belongs to the pirin family.Curated

Phylogenomic databases

eggNOGiENOG4105C2I Bacteria
COG1741 LUCA
HOGENOMiHOG000114205
InParanoidiP46852
KOiK06911
OMAiDAHHHFS
PhylomeDBiP46852

Family and domain databases

Gene3Di2.60.120.10, 3 hits
InterProiView protein in InterPro
IPR012093 Pirin
IPR003829 Pirin_N_dom
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PfamiView protein in Pfam
PF02678 Pirin, 1 hit
PIRSFiPIRSF006232 Pirin, 1 hit
SUPFAMiSSF51182 SSF51182, 1 hit

Sequencei

Sequence statusi: Complete.

P46852-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYLRKANER GHANHGWLDS WHTFSFANYY DPNFMGFSAL RVINDDVIEA
60 70 80 90 100
GQGFGTHPHK DMEILTYVLE GTVEHQDSMG NKEQVPAGEF QIMSAGTGIR
110 120 130 140 150
HSEYNPSSTE RLHLYQIWIM PEENGITPRY EQRRFDAVQG KQLVLSPDAR
160 170 180 190 200
DGSLKVHQDM ELYRWALLKD EQSVHQIAAE RRVWIQVVKG NVTINGVKAS
210 220 230
TSDGLAIWDE QAISIHADSD SEVLLFDLPP V
Length:231
Mass (Da):26,279
Last modified:November 1, 1995 - v1
Checksum:i62E5B21331686935
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA58237.1
U00096 Genomic DNA Translation: AAC76464.1
AP009048 Genomic DNA Translation: BAE77854.1
PIRiB65140
RefSeqiNP_417896.1, NC_000913.3
WP_000639811.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76464; AAC76464; b3439
BAE77854; BAE77854; BAE77854
GeneIDi947945
KEGGiecj:JW3402
eco:b3439
PATRICifig|1411691.4.peg.3290

Similar proteinsi

Entry informationi

Entry nameiYHHW_ECOLI
AccessioniPrimary (citable) accession number: P46852
Secondary accession number(s): Q2M7A2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health