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Protein

Quercetin 2,3-dioxygenase

Gene

yhhW

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has quercetin 2,3-dioxygenase activity in vitro. Its physiological role is unknown; however, may provide a mechanism that would avoid inhibition of key cellular proteins, such as DNA gyrase, by quercetin.1 Publication

Catalytic activityi

Quercetin + O2 = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H+.1 Publication

Cofactori

Zn2+1 Publication, Co2+1 Publication, Fe2+1 PublicationNote: Binds 1 divalent metal cation, Zn2+, Co2+ or Fe2+.1 Publication

Enzyme regulationi

Inhibited by kojic acid, sodium diethyldithiocarbamate and 1,10-phenanthroline monohydrochloride.1 Publication

Pathwayi: quercetin degradation

This protein is involved in the pathway quercetin degradation, which is part of Flavonoid metabolism.
View all proteins of this organism that are known to be involved in the pathway quercetin degradation and in Flavonoid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi57Divalent metal cation1
Metal bindingi59Divalent metal cation1
Metal bindingi101Divalent metal cation1
Metal bindingi103Divalent metal cationBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • quercetin 2,3-dioxygenase activity Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Copper, Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G7756-MONOMER.
ECOL316407:JW3402-MONOMER.
MetaCyc:G7756-MONOMER.
UniPathwayiUPA00724.

Names & Taxonomyi

Protein namesi
Recommended name:
Quercetin 2,3-dioxygenase (EC:1.13.11.24)
Short name:
Quercetinase
Alternative name(s):
Pirin-like protein YhhW
Gene namesi
Name:yhhW
Ordered Locus Names:b3439, JW3402
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12943. yhhW.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002140631 – 231Quercetin 2,3-dioxygenaseAdd BLAST231

Proteomic databases

PaxDbiP46852.
PRIDEiP46852.

Interactioni

Protein-protein interaction databases

BioGridi4261175. 17 interactors.
DIPiDIP-12356N.
IntActiP46852. 9 interactors.
STRINGi511145.b3439.

Structurei

Secondary structure

1231
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Helixi7 – 9Combined sources3
Beta strandi10 – 14Combined sources5
Beta strandi16 – 24Combined sources9
Beta strandi40 – 48Combined sources9
Beta strandi53 – 59Combined sources7
Beta strandi63 – 80Combined sources18
Beta strandi82 – 86Combined sources5
Beta strandi90 – 94Combined sources5
Beta strandi99 – 104Combined sources6
Beta strandi112 – 119Combined sources8
Beta strandi129 – 133Combined sources5
Beta strandi139 – 148Combined sources10
Helixi150 – 152Combined sources3
Beta strandi157 – 159Combined sources3
Beta strandi161 – 167Combined sources7
Beta strandi172 – 175Combined sources4
Beta strandi182 – 194Combined sources13
Beta strandi197 – 200Combined sources4
Beta strandi204 – 209Combined sources6
Beta strandi213 – 228Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TQ5X-ray1.76A1-231[»]
ProteinModelPortaliP46852.
SMRiP46852.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP46852.

Family & Domainsi

Domaini

Is composed of two structurally similar domains arranged face to face.1 Publication

Sequence similaritiesi

Belongs to the pirin family.Curated

Phylogenomic databases

eggNOGiENOG4105C2I. Bacteria.
COG1741. LUCA.
HOGENOMiHOG000114205.
InParanoidiP46852.
KOiK06911.
OMAiGFPTHGH.
PhylomeDBiP46852.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR012093. Pirin.
IPR003829. Pirin_N_dom.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR13903. PTHR13903. 1 hit.
PfamiPF02678. Pirin. 1 hit.
[Graphical view]
PIRSFiPIRSF006232. Pirin. 1 hit.
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

P46852-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYLRKANER GHANHGWLDS WHTFSFANYY DPNFMGFSAL RVINDDVIEA
60 70 80 90 100
GQGFGTHPHK DMEILTYVLE GTVEHQDSMG NKEQVPAGEF QIMSAGTGIR
110 120 130 140 150
HSEYNPSSTE RLHLYQIWIM PEENGITPRY EQRRFDAVQG KQLVLSPDAR
160 170 180 190 200
DGSLKVHQDM ELYRWALLKD EQSVHQIAAE RRVWIQVVKG NVTINGVKAS
210 220 230
TSDGLAIWDE QAISIHADSD SEVLLFDLPP V
Length:231
Mass (Da):26,279
Last modified:November 1, 1995 - v1
Checksum:i62E5B21331686935
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58237.1.
U00096 Genomic DNA. Translation: AAC76464.1.
AP009048 Genomic DNA. Translation: BAE77854.1.
PIRiB65140.
RefSeqiNP_417896.1. NC_000913.3.
WP_000639811.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76464; AAC76464; b3439.
BAE77854; BAE77854; BAE77854.
GeneIDi947945.
KEGGiecj:JW3402.
eco:b3439.
PATRICi32122318. VBIEscCol129921_3536.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58237.1.
U00096 Genomic DNA. Translation: AAC76464.1.
AP009048 Genomic DNA. Translation: BAE77854.1.
PIRiB65140.
RefSeqiNP_417896.1. NC_000913.3.
WP_000639811.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TQ5X-ray1.76A1-231[»]
ProteinModelPortaliP46852.
SMRiP46852.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261175. 17 interactors.
DIPiDIP-12356N.
IntActiP46852. 9 interactors.
STRINGi511145.b3439.

Proteomic databases

PaxDbiP46852.
PRIDEiP46852.

Protocols and materials databases

DNASUi947945.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76464; AAC76464; b3439.
BAE77854; BAE77854; BAE77854.
GeneIDi947945.
KEGGiecj:JW3402.
eco:b3439.
PATRICi32122318. VBIEscCol129921_3536.

Organism-specific databases

EchoBASEiEB2777.
EcoGeneiEG12943. yhhW.

Phylogenomic databases

eggNOGiENOG4105C2I. Bacteria.
COG1741. LUCA.
HOGENOMiHOG000114205.
InParanoidiP46852.
KOiK06911.
OMAiGFPTHGH.
PhylomeDBiP46852.

Enzyme and pathway databases

UniPathwayiUPA00724.
BioCyciEcoCyc:G7756-MONOMER.
ECOL316407:JW3402-MONOMER.
MetaCyc:G7756-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP46852.
PROiP46852.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR012093. Pirin.
IPR003829. Pirin_N_dom.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR13903. PTHR13903. 1 hit.
PfamiPF02678. Pirin. 1 hit.
[Graphical view]
PIRSFiPIRSF006232. Pirin. 1 hit.
SUPFAMiSSF51182. SSF51182. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiYHHW_ECOLI
AccessioniPrimary (citable) accession number: P46852
Secondary accession number(s): Q2M7A2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Quercetin is a flavonoid compound synthesized by a variety of plants, including foods for human consumption.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.