P46837 (YHGF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein YhgF

Gene names

Name:	yhgF
Ordered Locus Names:	b3407, JW3370

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	773 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities	Contains 1 S1 motif domain.
Sequence caution	The sequence AAA58204.1 differs from that shown. Reason: Wrong choice of frame. The sequence AAA58205.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAA58205.1 differs from that shown. Reason: Frameshift at positions 12 and 66.

Ontologies

Keywords
Ligand	RNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleobase-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
valS	P07118	1	EBI-554743,EBI-559242

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 773	773	Protein YhgF		PRO_0000215104
Regions
	Domain	651 – 720	70	S1 motif
Experimental info
	Sequence conflict	754 – 755	2	QP → HA in AAA58205. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P46837 [UniParc]. Last modified July 15, 1999. Version 3. Checksum: EA54D9ED952A8229	FASTA	773	85,120
	10 20 30 40 50 60 MMNDSFCRII AGEIQARPEQ VDAAVRLLDE GNTVPFIARY RKEITGGLDD TQLRNLETRL 70 80 90 100 110 120 SYLRELEERR QAILKSISEQ GKLTDDLAKA INATLSKTEL EDLYLPYKPK RRTRGQIAIE 130 140 150 160 170 180 AGLEPLADLL WSDPSHTPEV AAAQYVYADK GVADTKAALD GARYILMERF AEDAALLAKV 190 200 210 220 230 240 RDYLWKNAHL VSTVVSGKEE EGAKFRDYFD HHEPLSTVPS HRALAMFRGR NEGVLQLSLN 250 260 270 280 290 300 ADPQFDEPPK ESYCEQIIMD HLGLRLNNAP ADSWRKGVVS WTWRIKVLMH LETELMGTVR 310 320 330 340 350 360 ERAEDEAINV FARNLHDLLM AAPAGLRATM GLDPGLRTGV KVAVVDATGK LVATDTIYPH 370 380 390 400 410 420 TGQAAKAAMT VAALCEKHNV ELVAIGNGTA SRETERFYLD VQKQFPKVTA QKVIVSEAGA 430 440 450 460 470 480 SVYSASELAA QEFPDLDVSL RGAVSIARRL QDPLAELVKI DPKSIGVGQY QHDVSQTQLA 490 500 510 520 530 540 RKLDAVVEDC VNAVGVDLNT ASVPLLTRVA GLTRMMAQNI VAWRDENGQF QNRQQLLKVS 550 560 570 580 590 600 RLGPKAFEQC AGFLRINHGD NPLDASTVHP EAYPVVERIL AATQQALKDL MGNSSELRNL 610 620 630 640 650 660 KASDFTDEKF GVPTVTDIIK ELEKPGRDPR PEFKTAQFAD GVETMNDLQP GMILEGAVTN 670 680 690 700 710 720 VTNFGAFVDI GVHQDGLVHI SSLSNKFVED PHTVVKAGDI VKVKVLEVDL QRKRIALTMR 730 740 750 760 770 LDEQPGETNA RRGGGNERPQ NNRPAAKPRG REAQPAGNSA MMDALAAAMG KKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography." Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B. Electrophoresis 20:2181-2195(1999) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: B / BL21.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18997 Genomic DNA. Translation: AAA58204.1. Sequence problems. U18997 Genomic DNA. Translation: AAA58205.1. Sequence problems. U00096 Genomic DNA. Translation: AAC76432.2. AP009048 Genomic DNA. Translation: BAE77884.1.
PIR	B65136.
RefSeq	NP_417866.4. NC_000913.2. YP_492025.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P46837.
SMR	P46837. Positions 4-726.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-12337N.
IntAct	P46837. 9 interactions.
MINT	MINT-1288997.
STRING	511145.b3407.
Proteomic databases
PaxDb	P46837.
PRIDE	P46837.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76432; AAC76432; b3407. BAE77884; BAE77884; BAE77884.
GeneID	12932731. 947911.
KEGG	ecj:Y75_p3769. eco:b3407.
PATRIC	32122250. VBIEscCol129921_3502.
Organism-specific databases
EchoBASE	EB2768.
EcoGene	EG12932. yhgF.
Phylogenomic databases
eggNOG	COG2183.
HOGENOM	HOG000270497.
KO	K06959.
OMA	YKQKRRT.
ProtClustDB	CLSK862831.
Enzyme and pathway databases
BioCyc	EcoCyc:G7746-MONOMER. ECOL316407:JW3370-MONOMER.
Gene expression databases
Genevestigator	P46837.
Family and domain databases
Gene3D	1.10.10.650. 1 hit. 1.10.150.310. 1 hit. 1.10.3500.10. 2 hits. 2.40.50.140. 1 hit. 3.30.420.140. 1 hit.
InterPro	IPR012340. NA-bd_OB-fold. IPR003029. Rbsml_prot_S1_RNA-bd_dom. IPR022967. RNA-binding_domain_S1. IPR023323. Tex-like_dom. IPR023319. Tex-like_HTH_dom. IPR018974. Tex-like_N. IPR023097. Tex_RuvX-like_dom. IPR006641. YqgF/RNaseH-like_dom. [Graphical view]
Pfam	PF00575. S1. 1 hit. PF09371. Tex_N. 1 hit. [Graphical view]
SMART	SM00316. S1. 1 hit. SM00732. YqgFc. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
PROSITE	PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YHGF_ECOLI

Accession

Primary (citable) accession number: P46837
Secondary accession number(s): P76689, Q2M772

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	July 15, 1999
Last modified:	May 1, 2013
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program