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P46831 (ACEA_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isocitrate lyase

Short name=ICL
Short name=Isocitrase
Short name=Isocitratase
EC=4.1.3.1
Gene names
Name:aceA
Ordered Locus Names:ML1985
OrganismMycobacterium leprae (strain TN) [Complete proteome] [HAMAP]
Taxonomic identifier272631 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length606 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase-positive serine pathway By similarity.

Catalytic activity

Isocitrate = succinate + glyoxylate.

Cofactor

Divalent cations By similarity.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.

Sequence caution

The sequence CAC30940.1 differs from that shown. Reason: Frameshift at position 569.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentCytoplasm
   Molecular functionLyase
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionisocitrate lyase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 606606Isocitrate lyase
PRO_0000068778

Sites

Active site2111 By similarity

Experimental info

Sequence conflict1581R → L in CAA86357. Ref.1
Sequence conflict5691V → L in CAA86357. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P46831 [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: D82CCCADD6B6D384

FASTA60667,601
        10         20         30         40         50         60 
MAIMDTNTEV HTLFTQEVAA TQQYFDDPRF AGIIRLYTAR QVVEQRGTIP TDYTVARDAA 

        70         80         90        100        110        120 
TAFYARLREL FAAGKSVTTF GPYSPGQAVS LKRMGIEAIY LGGWATSAKG SITEDPGPDL 

       130        140        150        160        170        180 
ASYPLSQVPD DAAVLVRALL AADRNQQYLR LHMTEQQRAA TPAYDYRPFI IADADTGHGG 

       190        200        210        220        230        240 
DSHVRNLIRR FVEIGVPGYH IEDQRPGTKK CGHQGGKVLV PSDEQIKRLN AARFQLDIMR 

       250        260        270        280        290        300 
VPGIIVARTD AEAANLIDSR ADERDQPFLL GATNLKIPSY KACFLALVRC FYELGVKELH 

       310        320        330        340        350        360 
GHLLYALGDG EYAAASAWLD RQGILAQVSG TVNAWQENGK QSIDDLFEQV EYRLLAAWEK 

       370        380        390        400        410        420 
DAGLMTYGEA VEEMLQFGES EGELIGMSPE EWRRFVGRAS LYAAREKAKE LGVDPGWDCE 

       430        440        450        460        470        480 
LAKTPEGYYQ IRGGIQYAIA KSLAAAPFAD ILWMETKTAD LADARQFAEA IHAEFPEQML 

       490        500        510        520        530        540 
AYNLSPSFNW DTTGMSDEEM KRFPEELGKM GFVFNFITYG GHQIDGVAAE EFATALRQDG 

       550        560        570        580        590        600 
MLALARLQRK MRLVESPYRT PQTLVGGPVA MQRWLRPPDV RRQPRPWAKV RPSTSILCRP 


RCRRSC 

« Hide

References

« Hide 'large scale' references
[1]"The Mycobacterium leprae genome: systematic sequence analysis identifies key catabolic enzymes, ATP-dependent transport systems and a novel polA locus associated with genomic variability."
Fsihi H., Cole S.T.
Mol. Microbiol. 16:909-919(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Massive gene decay in the leprosy bacillus."
Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. expand/collapse author list , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G.
Nature 409:1007-1011(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TN.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46257 Genomic DNA. Translation: CAA86357.1.
AL583924 Genomic DNA. Translation: CAC30940.1. Frameshift.
PIRD87157.
S77654.
RefSeqNP_302337.1. NC_002677.1.

3D structure databases

ProteinModelPortalP46831.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272631.ML1985.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC30940; CAC30940; CAC30940.
GeneID909102.
KEGGmle:ML1985.
PATRIC18057640. VBIMycLep78757_3744.

Organism-specific databases

LepromaML1985.
CMRSearch...

Phylogenomic databases

eggNOGCOG2224.
HOGENOMHOG000238475.
KOK01637.
OrthoDBEOG689HMX.

Enzyme and pathway databases

UniPathwayUPA00703; UER00719.

Family and domain databases

Gene3D3.20.20.60. 2 hits.
InterProIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamPF00463. ICL. 2 hits.
[Graphical view]
PIRSFPIRSF001362. Isocit_lyase. 1 hit.
SUPFAMSSF51621. SSF51621. 2 hits.
PROSITEPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACEA_MYCLE
AccessionPrimary (citable) accession number: P46831
Secondary accession number(s): Q9CBH0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: April 27, 2001
Last modified: May 14, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways