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Protein

Isocitrate lyase

Gene

aceA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the persistence and virulence of Mycobacterium. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates.By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity

Cofactori

Mg2+By similarity

Pathway:iglyoxylate cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase (aceA)
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi173 – 1731MagnesiumBy similarity
Active sitei211 – 2111Proton acceptorBy similarity
Binding sitei248 – 2481SubstrateBy similarity
Binding sitei518 – 5181SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyaseBy similarity (EC:4.1.3.1By similarity)
Short name:
ICLBy similarity
Alternative name(s):
IsocitraseBy similarity
IsocitrataseBy similarity
Gene namesi
Name:aceA
Ordered Locus Names:ML1985
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
ProteomesiUP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML1985.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 606606Isocitrate lyasePRO_0000068778Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi272631.ML1985.

Structurei

3D structure databases

ProteinModelPortaliP46831.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni102 – 1043Substrate bindingBy similarity
Regioni212 – 2132Substrate bindingBy similarity
Regioni483 – 4875Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2224.
HOGENOMiHOG000238475.
OrthoDBiEOG689HMX.

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 2 hits.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 2 hits.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46831-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIMDTNTEV HTLFTQEVAA TQQYFDDPRF AGIIRLYTAR QVVEQRGTIP
60 70 80 90 100
TDYTVARDAA TAFYARLREL FAAGKSVTTF GPYSPGQAVS LKRMGIEAIY
110 120 130 140 150
LGGWATSAKG SITEDPGPDL ASYPLSQVPD DAAVLVRALL AADRNQQYLR
160 170 180 190 200
LHMTEQQRAA TPAYDYRPFI IADADTGHGG DSHVRNLIRR FVEIGVPGYH
210 220 230 240 250
IEDQRPGTKK CGHQGGKVLV PSDEQIKRLN AARFQLDIMR VPGIIVARTD
260 270 280 290 300
AEAANLIDSR ADERDQPFLL GATNLKIPSY KACFLALVRC FYELGVKELH
310 320 330 340 350
GHLLYALGDG EYAAASAWLD RQGILAQVSG TVNAWQENGK QSIDDLFEQV
360 370 380 390 400
EYRLLAAWEK DAGLMTYGEA VEEMLQFGES EGELIGMSPE EWRRFVGRAS
410 420 430 440 450
LYAAREKAKE LGVDPGWDCE LAKTPEGYYQ IRGGIQYAIA KSLAAAPFAD
460 470 480 490 500
ILWMETKTAD LADARQFAEA IHAEFPEQML AYNLSPSFNW DTTGMSDEEM
510 520 530 540 550
KRFPEELGKM GFVFNFITYG GHQIDGVAAE EFATALRQDG MLALARLQRK
560 570 580 590 600
MRLVESPYRT PQTLVGGPVA MQRWLRPPDV RRQPRPWAKV RPSTSILCRP

RCRRSC
Length:606
Mass (Da):67,601
Last modified:April 27, 2001 - v2
Checksum:iD82CCCADD6B6D384
GO

Sequence cautioni

The sequence CAC30940.1 differs from that shown. Reason: Frameshift at position 569. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti158 – 1581R → L in CAA86357 (PubMed:7476188).Curated
Sequence conflicti569 – 5691V → L in CAA86357 (PubMed:7476188).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46257 Genomic DNA. Translation: CAA86357.1.
AL583924 Genomic DNA. Translation: CAC30940.1. Frameshift.
PIRiD87157.
S77654.
RefSeqiWP_010908657.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC30940; CAC30940; CAC30940.
KEGGimle:ML1985.
PATRICi18057640. VBIMycLep78757_3744.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46257 Genomic DNA. Translation: CAA86357.1.
AL583924 Genomic DNA. Translation: CAC30940.1. Frameshift.
PIRiD87157.
S77654.
RefSeqiWP_010908657.1. NC_002677.1.

3D structure databases

ProteinModelPortaliP46831.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML1985.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC30940; CAC30940; CAC30940.
KEGGimle:ML1985.
PATRICi18057640. VBIMycLep78757_3744.

Organism-specific databases

LepromaiML1985.

Phylogenomic databases

eggNOGiCOG2224.
HOGENOMiHOG000238475.
OrthoDBiEOG689HMX.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 2 hits.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 2 hits.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Mycobacterium leprae genome: systematic sequence analysis identifies key catabolic enzymes, ATP-dependent transport systems and a novel polA locus associated with genomic variability."
    Fsihi H., Cole S.T.
    Mol. Microbiol. 16:909-919(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiACEA_MYCLE
AccessioniPrimary (citable) accession number: P46831
Secondary accession number(s): Q9CBH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: April 27, 2001
Last modified: July 22, 2015
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.