Reviewed, UniProtKB/Swiss-Prot P46817 (KATG_MYCBO)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase
      Short name=CP
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Mb1943c

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	740 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. May play a role in the intracellular survival of mycobacteria.
Catalytic activity	2 H(2)O(2) = O(2) + 2 H(2)O. Donor + H(2)O(2) = oxidized donor + 2 H(2)O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 740	740	Catalase-peroxidase		PRO_0000055571
Sites
	Active site	108	1	Proton acceptor By similarity
	Metal binding	270	1	Iron (heme axial ligand) By similarity
	Site	104	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	107 ↔ 229		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-255) By similarity
	Cross-link	229 ↔ 255		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-107) By similarity
Experimental info
	Sequence conflict	234	1	G → A in CAA58266. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P46817-1 [UniParc]. Last modified July 11, 2003. Version 2. Checksum: 8231F1E473397908	FASTA	740	80,562
	10 20 30 40 50 60 MPEQHPPITE TTTGAASNGC PVVGHMKYPV EGGGNQDWWP NRLNLKVLHQ NPAVADPMGA 70 80 90 100 110 120 AFDYAAEVAT IDVDALTRDI EEVMTTSQPW WPADYGHYGP LFIRMAWHAA GTYRIHDGRG 130 140 150 160 170 180 GAGGGMQRFA PLNSWPDNAS LDKARRLLWP VKKKYGKKLS WADLIVFAGN CALESMGFKT 190 200 210 220 230 240 FGFGFGRVDQ WEPDEVYWGK EATWLGDERY SGKRDLENPL AAVQMGLIYV NPEGPNGNPD 250 260 270 280 290 300 PMAAAVDIRE TFRRMAMNDV ETAALIVGGH TFGKTHGAGP ADLVGPEPEA APLEQMGLGW 310 320 330 340 350 360 KSSYGTGTGK DAITSGIEVV WTNTPTKWDN SFLEILYGYE WELTKSPAGA WQYTAKDGAG 370 380 390 400 410 420 AGTIPDPFGG PGRSPTMLAT DLSLRVDPIY ERITRRWLEH PEELADEFAK AWYKLIHRDM 430 440 450 460 470 480 GPVARYLGPL VPKQTLLWQD PVPAVSHDLV GEAEIASLKS QILASGLTVS QLVSTAWAAA 490 500 510 520 530 540 SSFRGSDKRG GANGGRIRLQ PQVGWEVNDP DGDLRKVIRT LEEIQESFNS AAPGNIKVSF 550 560 570 580 590 600 ADLVVLGGCA AIEKAAKAAG HNITVPFTPG RTDASQEQTD VESFAVLEPK ADGFRNYLGK 610 620 630 640 650 660 GNPLPAEYML LDKANLLTLS APEMTVLVGG LRVLGANYKR LPLGVFTEAS ESLTNDFFVN 670 680 690 700 710 720 LLDMGITWEP SPADDGTYQG KDGSGKVKWT GSRVDLVFGS NSELRALVEV YGADDAQPKF 730 740 VQDFVAAWDK VMNLDRFDVR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Missense mutations in the catalase-peroxidase gene, katG, are associated with isoniazid resistance in Mycobacterium tuberculosis." Heym B., Alzari P.M., Honore N., Cole S.T. Mol. Microbiol. 15:235-245(1995) [PubMed: 7746145] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BCG.
[2]	"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.
[3]	"Catalase-peroxidase of Mycobacterium bovis BCG converts isoniazid to isonicotinamide, but not to isonicotinic acid: differentiation parameter between enzymes of Mycobacterium bovis BCG and Mycobacterium tuberculosis." Kang S.-K., Lee J.-H., Lee Y.-C., Kim C.-H. Biochim. Biophys. Acta 1760:724-729(2006) [PubMed: 16563633] [Abstract] Cited for: FUNCTION, SUBUNIT.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X83277 Genomic DNA. Translation: CAA58266.1. BX248340 Genomic DNA. Translation: CAD94645.1.
RefSeq	NP_855594.1.
3D structure databases
HSSP	HSSP built from PDB template 1MWV based on UniProtKB Q939D2.
SMR	P46817. Positions 26-740.
ModBase	Search...
Protein family/group databases
PeroxiBase	2434. MboCP01_BCG. 4578. MboCP01_AF2122.
Genome annotation databases
GeneID	1093267.
GenomeReviews	Gene locus Mb1943c in contig BX248333_GR.
KEGG	mbo:Mb1943c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P46817.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_MYCBO

Accession

Primary (citable) accession number: P46817

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	July 11, 2003
Last modified:	November 25, 2008
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)