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P46814

- DAPF_MYCLE

UniProt

P46814 - DAPF_MYCLE

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei11 – 111SubstrateUniRule annotation
    Binding sitei44 – 441SubstrateUniRule annotation
    Binding sitei78 – 781SubstrateUniRule annotation
    Active sitei87 – 871Proton donor/acceptorUniRule annotation
    Binding sitei167 – 1671SubstrateUniRule annotation
    Sitei169 – 1691Important for catalytic activityUniRule annotation
    Binding sitei203 – 2031SubstrateUniRule annotation
    Sitei221 – 2211Important for catalytic activityUniRule annotation
    Active sitei230 – 2301Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:ML0996
    ORF Names:B2235_C3_233
    OrganismiMycobacterium leprae (strain TN)
    Taxonomic identifieri272631 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000806: Chromosome

    Organism-specific databases

    LepromaiML0996.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 296296Diaminopimelate epimerasePRO_0000149852Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi87 ↔ 230

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi272631.ML0996.

    Structurei

    3D structure databases

    ProteinModelPortaliP46814.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni87 – 893Substrate bindingUniRule annotation
    Regioni221 – 2222Substrate bindingUniRule annotation
    Regioni231 – 2322Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220467.
    KOiK01778.
    OMAiLIVEPPY.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46814-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIFAKGHGTQ NDFVVLPDVE ADVTFTAAQV AALCNRRQGL GADGVLRVTT    50
    AGAAVTAGVL EHLPDGVSCS DWYMDYRNAD GSVAQMCGNG VRVFAHYLRA 100
    SGLESCDEFV VGSLAGPRLV NVHHVDELNA DVTVDMGKAN LLGSGGPAFA 150
    VTVGGRRFSG VAVDVGNPHL ACMDPQLSLE ELAALDLGAP VHLDRVQFPD 200
    GVNIEVLTAP VDGMVQMRVH ERGVGETRSC GTGTVAAAVA ALASAGADTG 250
    TLTVRVPGGD VVITITDVTS YLRGPSVLVA HGELADAWWY SLARSC 296
    Length:296
    Mass (Da):30,640
    Last modified:November 1, 1995 - v1
    Checksum:iF0FBD68A0D5BAF5C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00019 Genomic DNA. Translation: AAA17279.1.
    AL583920 Genomic DNA. Translation: CAC31377.1.
    PIRiS72943.
    RefSeqiNP_301738.1. NC_002677.1.
    WP_010908062.1. NC_002677.1.

    Genome annotation databases

    EnsemblBacteriaiCAC31377; CAC31377; CAC31377.
    GeneIDi910020.
    KEGGimle:ML0996.
    PATRICi18053718. VBIMycLep78757_1804.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00019 Genomic DNA. Translation: AAA17279.1 .
    AL583920 Genomic DNA. Translation: CAC31377.1 .
    PIRi S72943.
    RefSeqi NP_301738.1. NC_002677.1.
    WP_010908062.1. NC_002677.1.

    3D structure databases

    ProteinModelPortali P46814.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272631.ML0996.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC31377 ; CAC31377 ; CAC31377 .
    GeneIDi 910020.
    KEGGi mle:ML0996.
    PATRICi 18053718. VBIMycLep78757_1804.

    Organism-specific databases

    Lepromai ML0996.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220467.
    KOi K01778.
    OMAi LIVEPPY.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Smith D.R., Robison K.
      Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TN.

    Entry informationi

    Entry nameiDAPF_MYCLE
    AccessioniPrimary (citable) accession number: P46814
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3