Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).UniRule annotation

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 158ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferaseUniRule annotation (EC:2.5.1.75UniRule annotation)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMAPP:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMATaseUniRule annotation
Isopentenyl-diphosphate:tRNA isopentenyltransferaseUniRule annotation
Short name:
IPP transferaseUniRule annotation
Short name:
IPPTUniRule annotation
Short name:
IPTaseUniRule annotation
Gene namesi
Name:miaAUniRule annotation
Ordered Locus Names:ML0995
ORF Names:B2235_C3_232
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0995.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 311311tRNA dimethylallyltransferasePRO_0000163940Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei103 – 1031Interaction with substrate tRNAUniRule annotation
Sitei124 – 1241Interaction with substrate tRNAUniRule annotation

Protein-protein interaction databases

STRINGi272631.ML0995.

Structurei

3D structure databases

ProteinModelPortaliP46811.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni10 – 156Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the IPP transferase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiHTRNYAK.
OrthoDBiEOG661HB3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.

Sequencei

Sequence statusi: Complete.

P46811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPLAIVGPT GVGKSELALD VIERLGGQVS VEIVNADAMQ LYRGMDIGTA
60 70 80 90 100
KLPVAARRGI PHHQLDVLDV TETATVASYQ RTAAADIEAI AARGAVPVVV
110 120 130 140 150
GGSMLYVQSL LDDWSFPGTD PAVRVRWEQQ LAEVGVVRLH AELARRDLAA
160 170 180 190 200
AAAILPTDGR RTVRALEVVE LTGKPFAASA PRIGAPRWDT VIVGLDCDRT
210 220 230 240 250
ILSERLARRI DSMFGQGLVD EVRMLLRWGL RDGVTASRAL GYAQVLTALD
260 270 280 290 300
AGGDADHLDE ARQQTYLGHR RYARRQRSWF HRDHRVHWLD VGTVDRVGVV
310
DDALRVWRNA S
Length:311
Mass (Da):34,054
Last modified:November 1, 1995 - v1
Checksum:iC4E1B5F3F3180AF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00019 Genomic DNA. Translation: AAA17278.1.
AL583920 Genomic DNA. Translation: CAC31376.1.
PIRiS72942.
RefSeqiNP_301737.1. NC_002677.1.
WP_010908061.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31376; CAC31376; CAC31376.
GeneIDi910017.
KEGGimle:ML0995.
PATRICi18053714. VBIMycLep78757_1802.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00019 Genomic DNA. Translation: AAA17278.1.
AL583920 Genomic DNA. Translation: CAC31376.1.
PIRiS72942.
RefSeqiNP_301737.1. NC_002677.1.
WP_010908061.1. NC_002677.1.

3D structure databases

ProteinModelPortaliP46811.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML0995.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC31376; CAC31376; CAC31376.
GeneIDi910017.
KEGGimle:ML0995.
PATRICi18053714. VBIMycLep78757_1802.

Organism-specific databases

LepromaiML0995.

Phylogenomic databases

eggNOGiCOG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiHTRNYAK.
OrthoDBiEOG661HB3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Smith D.R., Robison K.
    Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiMIAA_MYCLE
AccessioniPrimary (citable) accession number: P46811
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 11, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.