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Protein

GMP synthase [glutamine-hydrolyzing]

Gene

guaA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the synthesis of GMP from XMP.By similarity

Catalytic activityi

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.

Pathwayi: GMP biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes GMP from XMP (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. GMP synthase [glutamine-hydrolyzing] (guaA)
This subpathway is part of the pathway GMP biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GMP from XMP (L-Gln route), the pathway GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei93 – 931NucleophileBy similarity
Active sitei179 – 1791By similarity
Active sitei181 – 1811By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi233 – 2397ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00189; UER00296.

Protein family/group databases

MEROPSiC26.A07.

Names & Taxonomyi

Protein namesi
Recommended name:
GMP synthase [glutamine-hydrolyzing] (EC:6.3.5.2)
Alternative name(s):
GMP synthetase
Glutamine amidotransferase
Gene namesi
Name:guaA
Ordered Locus Names:ML0395
ORF Names:B1620_C2_205
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0395.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 529529GMP synthase [glutamine-hydrolyzing]PRO_0000140146Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi272631.ML0395.

Structurei

3D structure databases

ProteinModelPortaliP46810.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 205190Glutamine amidotransferase type-1Add
BLAST
Domaini206 – 403198GMPS ATP-PPaseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105CM0. Bacteria.
COG0518. LUCA.
COG0519. LUCA.
HOGENOMiHOG000223964.
KOiK01951.
OMAiMILVLDF.
OrthoDBiPOG091H00MK.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00344. GMP_synthase. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46810-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSAGLDQP EPSPRPVLVV DFGAQYAQLI ARRVREARVF SEVIPHTTSI
60 70 80 90 100
EEITARDPLA IVLSGGPASV YTEGAPQLDP ALFDLGLPVF GICYGFQAMA
110 120 130 140 150
QVLGGTVART KTSEYGRTEL KVLGGDLHSG LPGVQPVWMS HGDAVTAAPD
160 170 180 190 200
GFDVAASSSG APVAAFENRD RRLAGVQYHP EVMHTPHGQQ VLSRFLHDFA
210 220 230 240 250
GLDADWTAAN IAGVLVEQVR AQIGNGHAIC GLSGGVDSAV AAALVQRAIG
260 270 280 290 300
DRLTCIFVDH GLLRDGERGQ VQRDFVAATG AKLVTVDAAE TFLQALSGVT
310 320 330 340 350
NPEGKRKIIG RQFIRAFEGA VRDLLGDSTF DSGIEFLVQG TLYPDVVESG
360 370 380 390 400
GGSGTANIKS HHNVGGLPDN LRFKLVEPLR LLFKDEVRAV GRQLDLPEEI
410 420 430 440 450
VARQPFPGPG LGIRIVGEVT AERLDTLRRA DSIAREELTT AGLDYQIWQC
460 470 480 490 500
PVVLLADVRS VGVQGDNRSY GHPIVLRPVS SEDAMTADWT WVPYEVLECI
510 520
STRITNEVAE VNRVVLDITN KPPGTIEWE
Length:529
Mass (Da):56,723
Last modified:April 27, 2001 - v2
Checksum:i443EDE9525CDD949
GO

Sequence cautioni

The sequence AAC43222 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00015 Genomic DNA. Translation: AAC43222.1. Different initiation.
AL583918 Genomic DNA. Translation: CAC29903.1.
PIRiC86958.
S72813.
RefSeqiNP_301383.1. NC_002677.1.
WP_010907707.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC29903; CAC29903; CAC29903.
GeneIDi909317.
KEGGimle:ML0395.
PATRICi18051436. VBIMycLep78757_0667.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00015 Genomic DNA. Translation: AAC43222.1. Different initiation.
AL583918 Genomic DNA. Translation: CAC29903.1.
PIRiC86958.
S72813.
RefSeqiNP_301383.1. NC_002677.1.
WP_010907707.1. NC_002677.1.

3D structure databases

ProteinModelPortaliP46810.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML0395.

Protein family/group databases

MEROPSiC26.A07.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC29903; CAC29903; CAC29903.
GeneIDi909317.
KEGGimle:ML0395.
PATRICi18051436. VBIMycLep78757_0667.

Organism-specific databases

LepromaiML0395.

Phylogenomic databases

eggNOGiENOG4105CM0. Bacteria.
COG0518. LUCA.
COG0519. LUCA.
HOGENOMiHOG000223964.
KOiK01951.
OMAiMILVLDF.
OrthoDBiPOG091H00MK.

Enzyme and pathway databases

UniPathwayiUPA00189; UER00296.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00344. GMP_synthase. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUAA_MYCLE
AccessioniPrimary (citable) accession number: P46810
Secondary accession number(s): Q9CCU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: April 27, 2001
Last modified: September 7, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.