P46807 (METB_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cystathionine gamma-synthase
Short name=CGS
EC=2.5.1.48

Alternative name(s):
O-succinylhomoserine (thiol)-lyase

Gene names

Name:	metB
Ordered Locus Names:	ML2394

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-cystathionine from O-succinyl-L-homoserine: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the trans-sulfuration enzymes family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cystathionine gamma-synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Cystathionine gamma-synthase		PRO_0000114761
Amino acid modifications
	Modified residue	208	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46807 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 0EF3DB05A0B59148	FASTA	388	41,292
	10 20 30 40 50 60 MSEDYRGHHG ITGLATKAIH AGYRPDPATG AVNVPIYASS TFAQDGVGEL RGGFEYARTG 70 80 90 100 110 120 NPMRAALEAS LATVEEGVFA RAFSSGMAAS DCALRVMLRP GDHVIIPDDV YGGTFRLIDK 130 140 150 160 170 180 VFTQWNVDYT PVPLSDLDAV RAAITSRTRL IWVETPTNPL LSIADITSIG ELGKKHSVKV 190 200 210 220 230 240 LVDNTFASPA LQQPLMLGAD VVLHSTTKYI GGHSDVVGGA LVTNDEELDQ AFGFLQNGAG 250 260 270 280 290 300 AVPSPFDAYL TMRGLKTLVL RMQRHNENAI TVAEFLAGHP SVSAVLYPGL PSHPGHEVAA 310 320 330 340 350 360 RQMRGFGGMV SLRMRAGRLA AQDLCARTKV FTLAESLGGV ESLIEQPSAM THASTTGSQL 370 380 EVPDDLVRLS VGIEDVGDLL CDLKQALN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Smith D.R., Robison K. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U15183 Genomic DNA. Translation: AAA63036.1. AL583925 Genomic DNA. Translation: CAC31910.1.
PIR	F87208.
RefSeq	NP_302550.1. NC_002677.1.
3D structure databases
ProteinModelPortal	P46807.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000028489; EBMYCP00000028095; EBMYCG00000028484.
GeneID	908547.
GenomeReviews	Gene locus ML2394 in contig AL450380_GR.
KEGG	mle:ML2394.
NMPDR	fig|272631.1.peg.1422.
PATRIC	18059385. VBIMycLep78757_4607.
Organism-specific databases
Leproma	ML2394.
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016651.
HOGENOM	HBG754729.
OMA	CETPTNP.
ProtClustDB	PRK07811.
Enzyme and pathway databases
BioCyc	MLEP272631:ML2394-MONOMER.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K01739.
PANTHER	PTHR11808. Cys_Met_Meta_PP. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
PIRSF	PIRSF001434. CGS. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00868. CYS_MET_METAB_PP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METB_MYCLE

Accession

Primary (citable) accession number: P46807

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program