P46806 (DHOM_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine dehydrogenase
Short name=HDH
EC=1.1.1.3

Gene names

Name:	hom
Ordered Locus Names:	ML1129

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.
Sequence similarities	Belongs to the homoserine dehydrogenase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Isoleucine biosynthesis Methionine biosynthesis Threonine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro amino acid binding Inferred from electronic annotation. Source: InterPro homoserine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 441	441	Homoserine dehydrogenase		PRO_0000066701
Regions
	Domain	355 – 433	79	ACT
	Nucleotide binding	13 – 20	8	NADP By similarity
Sites
	Active site	207	1	Proton donor Potential
	Binding site	107	1	NADP By similarity
	Binding site	192	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46806 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 41EEC1939A5FD0F8	FASTA	441	45,606
	10 20 30 40 50 60 MFSDERTVGV AVLGLGNVGS EVVRIIEGSA DDLAARIGAP LMLRGIGVRR VAVDRGVPVD 70 80 90 100 110 120 LLTDNIEELV SRADVDIVVE VMGPVELSRK AILSALEHGK SVVTANKALL AASTGELAQA 130 140 150 160 170 180 AESAHVDLYF EAAVAGAIPV IRPLTQSLAG DTVLRVAGIV NGTTNYILSA MDSTGADYDS 190 200 210 220 230 240 ALAGARALGY AEADPTADVE GHDAAAKAAI LASIAFHTRV TADDVYREGI TKITPADFVS 250 260 270 280 290 300 ARALGCTIKL LFICERITAA DGQQRVSARV YPALVPMSHP LATVSGAFNA VVVEAEAAGR 310 320 330 340 350 360 LMFYGQGAGG APTASAVTGD LVMAARNRVL GSRGPKESKY AQLPMETIGF ISTRYYVSMN 370 380 390 400 410 420 VADKPGVLSG VAAEFAKREV SIAEVRQEGV VDDGGRRVGA RIVVVTHGAT DAALSETVDA 430 440 LADLDVVQGV TSVLRLEGIS L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Smith D.R., Robison K. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U15186 Genomic DNA. Translation: AAA63101.1. AL583920 Genomic DNA. Translation: CAC31510.1.
PIR	T09992.
RefSeq	NP_301823.1. NC_002677.1.
3D structure databases
ProteinModelPortal	P46806.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000028455; EBMYCP00000028061; EBMYCG00000028450.
GeneID	910223.
GenomeReviews	Gene locus ML1129 in contig AL450380_GR.
KEGG	mle:ML1129.
NMPDR	fig|272631.1.peg.695.
PATRIC	18054213. VBIMycLep78757_2051.
Organism-specific databases
Leproma	ML1129.
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016352.
HOGENOM	HBG527379.
OMA	VHPAMIP.
ProtClustDB	PRK06349.
Enzyme and pathway databases
BioCyc	MLEP272631:ML1129-MONOMER.
Family and domain databases
InterPro	IPR002912. ACT-bd. IPR005106. Asp/hSer_DH_NAD-bd. IPR016204. HDH. IPR001342. HDH_cat. IPR019811. HDH_CS. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00003.
Pfam	PF01842. ACT. 1 hit. PF00742. Homoserine_dh. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF000098. Homoser_dehydrog. 1 hit.
PROSITE	PS01042. HOMOSER_DHGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHOM_MYCLE

Accession

Primary (citable) accession number: P46806

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program