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P46805 (DDL_THEMA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:TM_0259
OrganismThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]
Taxonomic identifier243274 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length303 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence caution

The sequence AAA68950.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 303303D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177896

Regions

Domain99 – 293195ATP-grasp
Nucleotide binding125 – 17652ATP By similarity

Sites

Metal binding2481Magnesium or manganese 1 By similarity
Metal binding2601Magnesium or manganese 1 By similarity
Metal binding2601Magnesium or manganese 2 By similarity
Metal binding2621Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
P46805 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: A6057C711DA77725

FASTA30334,250
        10         20         30         40         50         60 
MRVALLMGGV SREREISLRS GERVKKALEK LGYEHTVFDV REDFLKKVDQ LKSFDVVFNV 

        70         80         90        100        110        120 
LHGTFGEDGT LQAILDFLGI RYTGSDAFSS MICFDKLVTY RFLKGTVEIP DFVEIKEFMK 

       130        140        150        160        170        180 
TSPLGYPCVV KPRREGSSIG VFVCESDEEF QHALKEDLPR YGSVIVQKYI PGREMTVSIL 

       190        200        210        220        230        240 
ETEKGFEILP VLELRPKRRF YDYVAKYTKG ETEFILPAPL NPSEERLVKE MALKAFVEAG 

       250        260        270        280        290        300 
CRGFGRVDGI FSDGRFYFLE INTVPGLTET SDLPASAKAG GIEFEELVEI ILKSAFLKEG 


VRV 

« Hide

References

« Hide 'large scale' references
[1]"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima."
Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. expand/collapse author list , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M.
Nature 399:323-329(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]"Cloning and sequencing of the gene coding for topoisomerase I from the extremely thermophilic eubacterium, Thermotoga maritima."
Bouthier de la Tour C., Kaltoum H., Portemer C., Confalonieri F., Huber R., Duguet M.
Biochim. Biophys. Acta 1264:279-283(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-118.
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000512 Genomic DNA. Translation: AAD35347.1.
U27841 Genomic DNA. Translation: AAA68950.1. Different initiation.
PIRB72400.
RefSeqNP_228072.1. NC_000853.1.
YP_007976608.1. NC_021214.1.
YP_008990767.1. NC_023151.1.

3D structure databases

ProteinModelPortalP46805.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243274.TM0259.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD35347; AAD35347; TM_0259.
GeneID15493861.
18092540.
897169.
KEGGtma:TM0259.
tmm:Tmari_0257.
PATRIC23935395. VBITheMar51294_0263.

Phylogenomic databases

eggNOGCOG1181.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK14571.

Enzyme and pathway databases

BioCycTMAR243274:GC6P-272-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_THEMA
AccessionPrimary (citable) accession number: P46805
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 30, 2000
Last modified: April 16, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways