P46804 (SPD2_NEPCL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Spidroin-2 Alternative name(s): Dragline silk fibroin 2
Organism	Nephila clavipes (Golden silk orbweaver)
Taxonomic identifier	6915 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Araneae › Araneomorphae › Entelegynae › Orbiculariae › Araneoidea › Nephilidae › Nephila

Protein attributes

Sequence length	627 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Spiders' major ampullate silk possesses unique characteristics of strength and elasticity. Fibroin consists of pseudocrystalline regions of antiparallel beta-sheet interspersed with elastic amorphous segments.
Subunit structure	Major subunit, with spidroin 1, of the dragline silk.
Subcellular location	Secreted › extracellular space.
Domain	Highly repetitive protein characterized by regions of polyalanine and glycine-rich repeating units.
Sequence similarities	Belongs to the silk fibroin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat
Molecular function	Silk protein
Gene Ontology (GO)
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 627	›627	Spidroin-2		PRO_0000221447
Regions
	Repeat	1 – 36	36	1
	Repeat	37 – 79	43	2
	Repeat	80 – 121	42	3
	Repeat	122 – 172	51	4
	Repeat	173 – 213	41	5
	Repeat	214 – 252	39	6
	Repeat	253 – 283	31	7
	Repeat	284 – 317	34	8
	Repeat	318 – 359	42	9
	Repeat	360 – 391	32	10
	Repeat	392 – 428	37	11
	Repeat	429 – 464	36	12
	Repeat	465 – 488	24	13
	Repeat	489 – 515	27	14
	Repeat	516 – 530	15	15
	Region	1 – 530	530	15 X approximate tandem repeats
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P46804 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: CB9B63779B2C594B	FASTA	627	54,184
	10 20 30 40 50 60 PGGYGPGQQG PGGYGPGQQG PSGPGSAAAA AAAAAAGPGG YGPGQQGPGG YGPGQQGPGR 70 80 90 100 110 120 YGPGQQGPSG PGSAAAAAAG SGQQGPGGYG PRQQGPGGYG QGQQGPSGPG SAAAASAAAS 130 140 150 160 170 180 AESGQQGPGG YGPGQQGPGG YGPGQQGPGG YGPGQQGPSG PGSAAAAAAA ASGPGQQGPG 190 200 210 220 230 240 GYGPGQQGPG GYGPGQQGPS GPGSAAAAAA AASGPGQQGP GGYGPGQQGP GGYGPGQQGL 250 260 270 280 290 300 SGPGSAAAAA AAGPGQQGPG GYGPGQQGPS GPGSAAAAAA AAAGPGGYGP GQQGPGGYGP 310 320 330 340 350 360 GQQGPSGAGS AAAAAAAGPG QQGLGGYGPG QQGPGGYGPG QQGPGGYGPG SASAAAAAAG 370 380 390 400 410 420 PGQQGPGGYG PGQQGPSGPG SASAAAAAAA AGPGGYGPGQ QGPGGYAPGQ QGPSGPGSAS 430 440 450 460 470 480 AAAAAAAAGP GGYGPGQQGP GGYAPGQQGP SGPGSAAAAA AAAAGPGGYG PAQQGPSGPG 490 500 510 520 530 540 IAASAASAGP GGYGPAQQGP AGYGPGSAVA ASAGAGSAGY GPGSQASAAA SRLASPDSGA 550 560 570 580 590 600 RVASAVSNLV SSGPTSSAAL SSVISNAVSQ IGASNPGLSG CDVLIQALLE IVSACVTILS 610 620 SSSIGQVNYG AASQFAQVVG QSVLSAF

References

[1]	"Isolation of a clone encoding a second dragline silk fibroin. Nephila clavipes dragline silk is a two-protein fiber." Hinman M.B., Lewis R.V. J. Biol. Chem. 267:19320-19324(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Web resources

Protein Spotlight

The tiptoe of an airbus - Issue 24 of July 2002

Cross-references

Sequence databases
EMBL GenBank DDBJ	M92913 mRNA. Translation: AAA29381.1.
PIR	A44112.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR021001. Spidroin_C. [Graphical view]
Pfam	PF11260. Spidroin_MaSp. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPD2_NEPCL

Accession

Primary (citable) accession number: P46804

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)