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P46804 (SPD2_NEPCL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Spidroin-2
Alternative name(s):
Dragline silk fibroin 2
OrganismNephila clavipes (Golden silk orbweaver)
Taxonomic identifier6915 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeAraneomorphaeEntelegynaeAraneoideaNephilidaeNephila

Protein attributes

Sequence length627 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Spiders' major ampullate silk possesses unique characteristics of strength and elasticity. Fibroin consists of pseudocrystalline regions of antiparallel beta-sheet interspersed with elastic amorphous segments.

Subunit structure

Major subunit, with spidroin 1, of the dragline silk.

Subcellular location

Secretedextracellular space.

Domain

Highly repetitive protein characterized by regions of polyalanine and glycine-rich repeating units.

Sequence similarities

Belongs to the silk fibroin family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainRepeat
   Molecular functionSilk protein
Gene Ontology (GO)
   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 627›627Spidroin-2
PRO_0000221447

Regions

Repeat1 – 36361
Repeat37 – 79432
Repeat80 – 121423
Repeat122 – 172514
Repeat173 – 213415
Repeat214 – 252396
Repeat253 – 283317
Repeat284 – 317348
Repeat318 – 359429
Repeat360 – 3913210
Repeat392 – 4283711
Repeat429 – 4643612
Repeat465 – 4882413
Repeat489 – 5152714
Repeat516 – 5301515
Region1 – 53053015 X approximate tandem repeats

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P46804 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: CB9B63779B2C594B

FASTA62754,184
        10         20         30         40         50         60 
PGGYGPGQQG PGGYGPGQQG PSGPGSAAAA AAAAAAGPGG YGPGQQGPGG YGPGQQGPGR 

        70         80         90        100        110        120 
YGPGQQGPSG PGSAAAAAAG SGQQGPGGYG PRQQGPGGYG QGQQGPSGPG SAAAASAAAS 

       130        140        150        160        170        180 
AESGQQGPGG YGPGQQGPGG YGPGQQGPGG YGPGQQGPSG PGSAAAAAAA ASGPGQQGPG 

       190        200        210        220        230        240 
GYGPGQQGPG GYGPGQQGPS GPGSAAAAAA AASGPGQQGP GGYGPGQQGP GGYGPGQQGL 

       250        260        270        280        290        300 
SGPGSAAAAA AAGPGQQGPG GYGPGQQGPS GPGSAAAAAA AAAGPGGYGP GQQGPGGYGP 

       310        320        330        340        350        360 
GQQGPSGAGS AAAAAAAGPG QQGLGGYGPG QQGPGGYGPG QQGPGGYGPG SASAAAAAAG 

       370        380        390        400        410        420 
PGQQGPGGYG PGQQGPSGPG SASAAAAAAA AGPGGYGPGQ QGPGGYAPGQ QGPSGPGSAS 

       430        440        450        460        470        480 
AAAAAAAAGP GGYGPGQQGP GGYAPGQQGP SGPGSAAAAA AAAAGPGGYG PAQQGPSGPG 

       490        500        510        520        530        540 
IAASAASAGP GGYGPAQQGP AGYGPGSAVA ASAGAGSAGY GPGSQASAAA SRLASPDSGA 

       550        560        570        580        590        600 
RVASAVSNLV SSGPTSSAAL SSVISNAVSQ IGASNPGLSG CDVLIQALLE IVSACVTILS 

       610        620 
SSSIGQVNYG AASQFAQVVG QSVLSAF 

« Hide

References

[1]"Isolation of a clone encoding a second dragline silk fibroin. Nephila clavipes dragline silk is a two-protein fiber."
Hinman M.B., Lewis R.V.
J. Biol. Chem. 267:19320-19324(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Web resources

Protein Spotlight

The tiptoe of an airbus - Issue 24 of July 2002

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M92913 mRNA. Translation: AAA29381.1.
PIRA44112.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR021001. Spidroin_C.
[Graphical view]
PfamPF11260. Spidroin_MaSp. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSPD2_NEPCL
AccessionPrimary (citable) accession number: P46804
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 19, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries