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Protein

Spidroin-2

Gene
N/A
Organism
Nephila clavipes (Golden silk orbweaver)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Spiders' major ampullate silk possesses unique characteristics of strength and elasticity. Fibroin consists of pseudocrystalline regions of antiparallel beta-sheet interspersed with elastic amorphous segments.

Keywords - Molecular functioni

Silk protein

Names & Taxonomyi

Protein namesi
Recommended name:
Spidroin-2
Alternative name(s):
Dragline silk fibroin 2
OrganismiNephila clavipes (Golden silk orbweaver)
Taxonomic identifieri6915 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeAraneomorphaeEntelegynaeAraneoideaNephilidaeNephila

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 627›627Spidroin-2PRO_0000221447Add
BLAST

Interactioni

Subunit structurei

Major subunit, with spidroin 1, of the dragline silk.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1 – 36361Add
BLAST
Repeati37 – 79432Add
BLAST
Repeati80 – 121423Add
BLAST
Repeati122 – 172514Add
BLAST
Repeati173 – 213415Add
BLAST
Repeati214 – 252396Add
BLAST
Repeati253 – 283317Add
BLAST
Repeati284 – 317348Add
BLAST
Repeati318 – 359429Add
BLAST
Repeati360 – 3913210Add
BLAST
Repeati392 – 4283711Add
BLAST
Repeati429 – 4643612Add
BLAST
Repeati465 – 4882413Add
BLAST
Repeati489 – 5152714Add
BLAST
Repeati516 – 5301515Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 53053015 X approximate tandem repeatsAdd
BLAST

Domaini

Highly repetitive protein characterized by regions of polyalanine and glycine-rich repeating units.

Sequence similaritiesi

Belongs to the silk fibroin family.Curated

Keywords - Domaini

Repeat

Family and domain databases

InterProiIPR021001. Spidroin_C.
[Graphical view]
PfamiPF11260. Spidroin_MaSp. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P46804-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
PGGYGPGQQG PGGYGPGQQG PSGPGSAAAA AAAAAAGPGG YGPGQQGPGG
60 70 80 90 100
YGPGQQGPGR YGPGQQGPSG PGSAAAAAAG SGQQGPGGYG PRQQGPGGYG
110 120 130 140 150
QGQQGPSGPG SAAAASAAAS AESGQQGPGG YGPGQQGPGG YGPGQQGPGG
160 170 180 190 200
YGPGQQGPSG PGSAAAAAAA ASGPGQQGPG GYGPGQQGPG GYGPGQQGPS
210 220 230 240 250
GPGSAAAAAA AASGPGQQGP GGYGPGQQGP GGYGPGQQGL SGPGSAAAAA
260 270 280 290 300
AAGPGQQGPG GYGPGQQGPS GPGSAAAAAA AAAGPGGYGP GQQGPGGYGP
310 320 330 340 350
GQQGPSGAGS AAAAAAAGPG QQGLGGYGPG QQGPGGYGPG QQGPGGYGPG
360 370 380 390 400
SASAAAAAAG PGQQGPGGYG PGQQGPSGPG SASAAAAAAA AGPGGYGPGQ
410 420 430 440 450
QGPGGYAPGQ QGPSGPGSAS AAAAAAAAGP GGYGPGQQGP GGYAPGQQGP
460 470 480 490 500
SGPGSAAAAA AAAAGPGGYG PAQQGPSGPG IAASAASAGP GGYGPAQQGP
510 520 530 540 550
AGYGPGSAVA ASAGAGSAGY GPGSQASAAA SRLASPDSGA RVASAVSNLV
560 570 580 590 600
SSGPTSSAAL SSVISNAVSQ IGASNPGLSG CDVLIQALLE IVSACVTILS
610 620
SSSIGQVNYG AASQFAQVVG QSVLSAF
Length:627
Mass (Da):54,184
Last modified:November 1, 1995 - v1
Checksum:iCB9B63779B2C594B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92913 mRNA. Translation: AAA29381.1.
PIRiA44112.

Cross-referencesi

Web resourcesi

Protein Spotlight

The tiptoe of an airbus - Issue 24 of July 2002

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92913 mRNA. Translation: AAA29381.1.
PIRiA44112.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR021001. Spidroin_C.
[Graphical view]
PfamiPF11260. Spidroin_MaSp. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation of a clone encoding a second dragline silk fibroin. Nephila clavipes dragline silk is a two-protein fiber."
    Hinman M.B., Lewis R.V.
    J. Biol. Chem. 267:19320-19324(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiSPD2_NEPCL
AccessioniPrimary (citable) accession number: P46804
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.