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Reviewed, UniProtKB/Swiss-Prot P46799 (TOP1_THEMA)

Last modified November 3, 2009. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase
Gene names
Name: topA
Ordered Locus Names: TM_0258
OrganismThermotoga maritima [Complete proteome] [HAMAP]
Taxonomic identifier2336 [NCBI]
Taxonomic lineageBacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

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Protein attributes

Sequence length633 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Subunit structure

Monomer By similarity.

Miscellaneous

When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.

Sequence similarities

Belongs to the prokaryotic type I/III topoisomerase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 633633DNA topoisomerase 1
PRO_0000145169

Regions

Zinc finger559 – 58022C4-type

Sites

Active site2881For DNA cleavage activity By similarity

Secondary structure

1 .................................................................................... 633
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P46799-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: F7262A044060CFE9

FASTA63372,695
        10         20         30         40         50         60 
MSKKVKKYIV VESPAKAKTI KSILGNEYEV FASMGHIIDL PKSKFGVDLE KDFEPEFAVI 

        70         80         90        100        110        120 
KGKEKVVEKL KDLAKKGELL IASDMDREGE AIAWHIARVT NTLGRKNRIV FSEITPRVIR 

       130        140        150        160        170        180 
EAVKNPREID MKKVRAQLAR RILDRIVGYS LSPVLWRNFK SNLSAGRVQS ATLKLVCDRE 

       190        200        210        220        230        240 
REILRFVPKK YHRITVNFDG LTAEIDVKEK KFFDAETLKE IQSIDELVVE EKKVSVKKFA 

       250        260        270        280        290        300 
PPEPFKTSTL QQEAYSKLGF SVSKTMMIAQ QLYEGVETKD GHIAFITYMR TDSTRVSDYA 

       310        320        330        340        350        360 
KEEARNLITE VFGEEYVGSK RERRKSNAKI QDAHEAIRPT NVFMTPEEAG KYLNSDQKKL 

       370        380        390        400        410        420 
YELIWKRFLA SQMKPSQYEE TRFVLRTKDG KYRFKGTVLK KIFDGYEKVW KTERNTGEFP 

       430        440        450        460        470        480 
FEEGESVKPV VVKIEEQETK PKPRYTEGSL VKEMERLGIG RPSTYASTIK LLLNRGYIKK 

       490        500        510        520        530        540 
IRGYLYPTIV GSVVMDYLEK KYSDVVSVSF TAEMEKDLDE VEQGKKTDKI VLREFYESFS 

       550        560        570        580        590        600 
SVFDRNDRIV VDFPTNQKCS CGKEMRLSFG KYGFYLKCEC GKTRSVKNDE IAVIDDGKIF 

       610        620        630 
LGRKDSESGS PDGRSVEGKG NLSEKRRKGK KGS

« Hide

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References

« Hide 'large scale' references
[1]"Cloning and sequencing of the gene coding for topoisomerase I from the extremely thermophilic eubacterium, Thermotoga maritima."
Bouthier de la Tour C., Kaltoum H., Portemer C., Confalonieri F., Huber R., Duguet M.
Biochim. Biophys. Acta 1264:279-283(1995) [PubMed: 8547314] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima."
Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. expand/collapse author list , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M.
Nature 399:323-329(1999) [PubMed: 10360571] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
+Additional computationally mapped references.

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Cross-references

Sequence databases

U27841 Genomic DNA. Translation: AAA68949.1.
AE000512 Genomic DNA. Translation: AAD35346.1.
PIRS62737.
RefSeqNP_228071.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2GAIX-ray1.70A/B3-633[»]
2GAJX-ray1.95A/B3-633[»]
ModBaseSearch...

Genome annotation databases

GeneID897168.
GenomeReviewsGene locus TM_0258 in contig AE000512_GR.
KEGGtma:TM0258.
NMPDRfig|243274.1.peg.255.
TIGRTM_0258.

Phylogenomic databases

HOGENOMP46799.
OMAAIAYHIA.

Enzyme and pathway databases

BioCycTMAR243274:TM_0258-MON.
BRENDA5.99.1.2. 16699.

Family and domain databases

InterProIPR003601. Topo_IA_2.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR000380. Topo_IA_core.
IPR003602. Topo_IA_DNA_bd.
IPR005733. TopoI_bac.
IPR006154. Toprim_dom_subgr.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameTOP1_THEMA
AccessionPrimary (citable) accession number: P46799
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 3, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents