P46794 (CBS_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cystathionine beta-synthase
EC=4.2.1.22

Alternative name(s):
Beta-thionase
Serine sulfhydrase

Gene names

Name:	cysB
ORF Names:	DDB_G0267386

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	L-serine + L-homocysteine = L-cystathionine + H2O.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family. Contains 2 CBS domains.
Sequence caution	The sequence AAA70099.1 differs from that shown. Reason: Frameshift at positions 413 and 424.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Domain	CBS domain Repeat
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro cysteine biosynthetic process via cystathionine Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: dictyBase
Molecular function	cystathionine beta-synthase activity Inferred from sequence or structural similarity. Source: dictyBase pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Cystathionine beta-synthase		PRO_0000167132
Regions
	Domain	374 – 430	57	CBS 1
	Domain	435 – 497	63	CBS 2
	Region	210 – 214	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	103	1	Pyridoxal phosphate By similarity
	Binding site	302	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	73	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	140	1	A → G in AAA70099. Ref.1
	Sequence conflict	300	1	G → A in AAA70099. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P46794 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: 31FD69EFB68B6432	FASTA	498	54,458
	10 20 30 40 50 60 MSAPEGPSKC TWTPNTTENT PHTTRRTPKK LIMDNILDNI GGTPLVRVNK VSSDLECELV 70 80 90 100 110 120 AKCEFFNAGG SVKDRIGHRM IVDAEESGRI KKGDTLIEPT SGNTGIGLAL TAAIKGYKMI 130 140 150 160 170 180 ITLPEKMSQE KVDVLKALGA EIIRTPTEAA FDAPESHIGV AKKLNSEIPN SHILDQYGNP 190 200 210 220 230 240 SNPLAHYDGT AEELLEQCEG KIDMIVCTAG TGGTITGIAR KIKERLPNCI VVGVDPHGSI 250 260 270 280 290 300 LAQPESLNNT NKSYKIEGIG YDFIPNVLER KLVDQWIKTD DKESFIMARR LIKEEGLLCG 310 320 330 340 350 360 GSSGSAMVGA LLAAKQLKKG QRCVVLLADS IRNYMTKHLN DDWLVDNGFV DPEYKTKDQQ 370 380 390 400 410 420 EEEKYHGATV KDLTLPKPIT ISATTTCAAA VQLLQQYGFD QLPVVSESKK VLGQLTLGNL 430 440 450 460 470 480 LSHIASKKAV PTDAVSKVMF RFTKNEKYIP ITQSTSLATL SKFFENHSSA IVTENDEIIS 490 IVTKIDLLTY LMKSQQKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing analysis of Dictyostelium discoideum cDNA encoding cystathionine beta synthase." Jho E., Kopachik W. Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: AX3.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U27536 mRNA. Translation: AAA70099.1. Frameshift. AAFI02000003 Genomic DNA. Translation: EAL73145.1.
RefSeq	XP_647501.1. XM_642409.1.
3D structure databases
ProteinModelPortal	P46794.
SMR	P46794. Positions 5-350.
ModBase	Search...
Protein-protein interaction databases
STRING	P46794.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191292; DDB0191292; DDB_G0267386.
GeneID	8616308.
GenomeReviews	Gene locus cysB in contig CM000150_GR.
KEGG	ddi:DDB_G0267386.
Organism-specific databases
dictyBase	DDB_G0267386. cysB.
Phylogenomic databases
eggNOG	KOG1252.
GeneTree	EPrGT00050000005200.
HOGENOM	HBG748215.
OMA	QFYEVEG.
PhylomeDB	P46794.
ProtClustDB	CLSZ2497306.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR001216. Cys_synth_BS. IPR005857. Cysta_beta_synth. IPR000644. Cysta_beta_synth_core. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01697.
Pfam	PF00571. CBS. 1 hit. PF00291. PALP. 1 hit. [Graphical view]
SMART	SM00116. CBS. 2 hits. [Graphical view]
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR01137. Cysta_beta. 1 hit.
PROSITE	PS51371. CBS. 2 hits. PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CBS_DICDI

Accession

Primary (citable) accession number: P46794
Secondary accession number(s): Q55FN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	December 4, 2007
Last modified:	November 16, 2011
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)