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Protein

Cystathionine beta-synthase

Gene

cysB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

L-serine + L-homocysteine = L-cystathionine + H2O.

Cofactori

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Cystathionine beta-synthase (cysB)
  2. Cystathionine gamma-lyase (cysA)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei103Pyridoxal phosphateBy similarity1
Binding sitei302Pyridoxal phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Cysteine biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-DDI-1614603. Cysteine formation from homocysteine.
UniPathwayiUPA00136; UER00201.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine beta-synthase (EC:4.2.1.22)
Alternative name(s):
Beta-thionase
Serine sulfhydrase
Gene namesi
Name:cysB
ORF Names:DDB_G0267386
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 1, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0267386. cysB.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001671321 – 498Cystathionine beta-synthaseAdd BLAST498

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei73N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiP46794.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0191292.

Structurei

3D structure databases

ProteinModelPortaliP46794.
SMRiP46794.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini374 – 430CBS 1PROSITE-ProRule annotationAdd BLAST57
Domaini435 – 497CBS 2PROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 214Pyridoxal phosphate bindingBy similarity5

Sequence similaritiesi

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

eggNOGiKOG1252. Eukaryota.
COG0031. LUCA.
InParanoidiP46794.
KOiK01697.
OMAiSYLSKFA.
PhylomeDBiP46794.

Family and domain databases

InterProiView protein in InterPro
IPR000644. CBS_dom.
IPR005857. Cysta_beta_synth.
IPR001216. P-phosphate_BS.
IPR001926. PLP-dep.
IPR036052. Trypto_synt_PLP_dependent.
PfamiView protein in Pfam
PF00571. CBS. 2 hits.
PF00291. PALP. 1 hit.
SMARTiView protein in SMART
SM00116. CBS. 2 hits.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01137. cysta_beta. 1 hit.
PROSITEiView protein in PROSITE
PS51371. CBS. 2 hits.
PS00901. CYS_SYNTHASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P46794-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAPEGPSKC TWTPNTTENT PHTTRRTPKK LIMDNILDNI GGTPLVRVNK
60 70 80 90 100
VSSDLECELV AKCEFFNAGG SVKDRIGHRM IVDAEESGRI KKGDTLIEPT
110 120 130 140 150
SGNTGIGLAL TAAIKGYKMI ITLPEKMSQE KVDVLKALGA EIIRTPTEAA
160 170 180 190 200
FDAPESHIGV AKKLNSEIPN SHILDQYGNP SNPLAHYDGT AEELLEQCEG
210 220 230 240 250
KIDMIVCTAG TGGTITGIAR KIKERLPNCI VVGVDPHGSI LAQPESLNNT
260 270 280 290 300
NKSYKIEGIG YDFIPNVLER KLVDQWIKTD DKESFIMARR LIKEEGLLCG
310 320 330 340 350
GSSGSAMVGA LLAAKQLKKG QRCVVLLADS IRNYMTKHLN DDWLVDNGFV
360 370 380 390 400
DPEYKTKDQQ EEEKYHGATV KDLTLPKPIT ISATTTCAAA VQLLQQYGFD
410 420 430 440 450
QLPVVSESKK VLGQLTLGNL LSHIASKKAV PTDAVSKVMF RFTKNEKYIP
460 470 480 490
ITQSTSLATL SKFFENHSSA IVTENDEIIS IVTKIDLLTY LMKSQQKN
Length:498
Mass (Da):54,458
Last modified:December 4, 2007 - v2
Checksum:i31FD69EFB68B6432
GO

Sequence cautioni

The sequence AAA70099 differs from that shown. Reason: Frameshift at positions 413 and 424.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti140A → G in AAA70099 (Ref. 1) Curated1
Sequence conflicti300G → A in AAA70099 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27536 mRNA. Translation: AAA70099.1. Frameshift.
AAFI02000003 Genomic DNA. Translation: EAL73145.1.
RefSeqiXP_647501.1. XM_642409.1.

Genome annotation databases

EnsemblProtistsiEAL73145; EAL73145; DDB_G0267386.
GeneIDi8616308.
KEGGiddi:DDB_G0267386.

Similar proteinsi

Entry informationi

Entry nameiCBS_DICDI
AccessioniPrimary (citable) accession number: P46794
Secondary accession number(s): Q55FN2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 4, 2007
Last modified: March 28, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome