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Protein

40S ribosomal protein S10-B

Gene

RPS10B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel (PubMed:22096102). eS10 plays a role as a positive regulator of the GCN2 kinase activity by stimulating GCN1-mediated GCN2 activation (PubMed:25437641).1 Publication1 Publication

Miscellaneous

Present with 7650 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for eS10 in yeast.Curated

GO - Molecular functioni

GO - Biological processi

  • cellular response to amino acid starvation Source: UniProtKB
  • cytoplasmic translation Source: SGD
  • positive regulation of protein kinase activity Source: UniProtKB
  • positive regulation of protein phosphorylation Source: UniProtKB
  • ribosomal small subunit assembly Source: GO_Central
  • rRNA export from nucleus Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-32911-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S10-B1 Publication
Alternative name(s):
Small ribosomal subunit protein eS10-B1 Publication
Gene namesi
Name:RPS10B1 Publication
Ordered Locus Names:YMR230W
ORF Names:YM9959.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR230W
SGDiS000004843 RPS10B

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001163771 – 10540S ribosomal protein S10-BAdd BLAST105

Post-translational modificationi

The N-terminus is not modified.1 Publication

Proteomic databases

MaxQBiP46784
PaxDbiP46784
PRIDEiP46784
TopDownProteomicsiP46784

PTM databases

iPTMnetiP46784

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102). eS10 interacts with GCN1 (via middle region); this interaction is direct and promotes GCN2 kinase activity (PubMed:25437641).1 Publication2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SRB5P325852EBI-16077,EBI-18032

Protein-protein interaction databases

BioGridi35408, 209 interactors
IntActiP46784, 13 interactors
MINTiP46784
STRINGi4932.YMR230W

Structurei

Secondary structure

1105
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 18Combined sources14
Beta strandi19 – 22Combined sources4
Beta strandi33 – 37Combined sources5
Helixi39 – 51Combined sources13
Beta strandi54 – 58Combined sources5
Beta strandi65 – 68Combined sources4
Helixi70 – 79Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4U3MX-ray3.00C0/c01-105[»]
ProteinModelPortaliP46784
SMRiP46784
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00440000034918
HOGENOMiHOG000216624
InParanoidiP46784
KOiK02947
OMAiYWHLTNE
OrthoDBiEOG092C5V0H

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR037447 Rps10
IPR005326 S10_plectin_N
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12146 PTHR12146, 1 hit
PfamiView protein in Pfam
PF03501 S10_plectin, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit

Sequencei

Sequence statusi: Complete.

P46784-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLMPKQERNK IHQYLFQEGV VVAKKDFNQA KHEEIDTKNL YVIKALQSLT
60 70 80 90 100
SKGYVKTQFS WQYYYYTLTE EGVEYLREYL NLPEHIVPGT YIQERNPSQR

PQRRY
Length:105
Mass (Da):12,738
Last modified:November 1, 1995 - v1
Checksum:iBE50B625869963CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49939 Genomic DNA Translation: CAA90201.1
BK006946 Genomic DNA Translation: DAA10129.1
PIRiS57597
RefSeqiNP_013957.1, NM_001182737.1

Genome annotation databases

EnsemblFungiiYMR230W; YMR230W; YMR230W
GeneIDi855270
KEGGisce:YMR230W

Similar proteinsi

Entry informationi

Entry nameiRS10B_YEAST
AccessioniPrimary (citable) accession number: P46784
Secondary accession number(s): D6W055
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health