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P46723 (HEM2_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Delta-aminolevulinic acid dehydratase

Short name=ALAD
Short name=ALADH
EC=4.2.1.24
Alternative name(s):
Porphobilinogen synthase
Gene names
Name:hemB
Ordered Locus Names:ML2419
ORF Names:B2168_C3_264
OrganismMycobacterium leprae (strain TN) [Complete proteome] [HAMAP]
Taxonomic identifier272631 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.

Catalytic activity

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.

Subunit structure

Homooctamer By similarity.

Sequence similarities

Belongs to the ALADH family.

Ontologies

Keywords
   Biological processHeme biosynthesis
Porphyrin biosynthesis
   LigandMagnesium
Metal-binding
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processprotoporphyrinogen IX biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

porphobilinogen synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329Delta-aminolevulinic acid dehydratase
PRO_0000140504

Sites

Active site2021Schiff-base intermediate with substrate By similarity
Active site2541Schiff-base intermediate with substrate By similarity
Metal binding2391Magnesium By similarity
Binding site2121Substrate 1 By similarity
Binding site2231Substrate 1 By similarity
Binding site2801Substrate 2 By similarity
Binding site3191Substrate 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
P46723 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 1E0EBA9498B8609C

FASTA32934,775
        10         20         30         40         50         60 
MSVSGYPRHR PRRLRSTPAM RRLVAQTSLE PRNLVLPMFV ADGIDELRPI ASMPGVVQHT 

        70         80         90        100        110        120 
RDSLRRAAVA AVDAGVGGLN LFGVPRDQDK DATGSAGVDP DGILNVALRD LAEDLGDATV 

       130        140        150        160        170        180 
LMADTCLDEF TDHGHCGVLD GQGRVDNDAT VARYVELAVA QAESGANVVG PSGMMDGQIG 

       190        200        210        220        230        240 
ALRDGLDSAG YADVAILAYA AKFSSAFYGP FREAVSCSLS GDRRTYQQEP GNAREALREI 

       250        260        270        280        290        300 
KLDLDEGADI IMIKPASGYL DVVATAAGVS PVPVAAYQVS GEYAMICAAA ANNWIDERAA 

       310        320 
VLESLTSIRR AGADIVFTYW AADVACWLS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00018 Genomic DNA. Translation: AAA17246.1.
AL583925 Genomic DNA. Translation: CAC31935.1.
PIRS72910.
RefSeqNP_302564.1. NC_002677.1.

3D structure databases

ProteinModelPortalP46723.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272631.ML2419.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC31935; CAC31935; CAC31935.
GeneID908581.
KEGGmle:ML2419.
PATRIC18059483. VBIMycLep78757_4656.

Organism-specific databases

LepromaML2419.
CMRSearch...

Phylogenomic databases

eggNOGCOG0113.
HOGENOMHOG000020323.
KOK01698.
OMADMILTYF.
OrthoDBEOG6VXFCB.

Enzyme and pathway databases

UniPathwayUPA00251; UER00318.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERPTHR11458. PTHR11458. 1 hit.
PfamPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSPR00144. DALDHYDRTASE.
SMARTSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM2_MYCLE
AccessionPrimary (citable) accession number: P46723
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 14, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways