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P46723

- HEM2_MYCLE

UniProt

P46723 - HEM2_MYCLE

Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.By similarity

    Catalytic activityi

    2 5-aminolevulinate = porphobilinogen + 2 H2O.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei202 – 2021Schiff-base intermediate with substrateBy similarity
    Binding sitei212 – 2121Substrate 1By similarity
    Binding sitei223 – 2231Substrate 1By similarity
    Metal bindingi239 – 2391MagnesiumBy similarity
    Active sitei254 – 2541Schiff-base intermediate with substrateBy similarity
    Binding sitei280 – 2801Substrate 2By similarity
    Binding sitei319 – 3191Substrate 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. porphobilinogen synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00251; UER00318.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
    Short name:
    ALAD
    Short name:
    ALADH
    Alternative name(s):
    Porphobilinogen synthase
    Gene namesi
    Name:hemB
    Ordered Locus Names:ML2419
    ORF Names:B2168_C3_264
    OrganismiMycobacterium leprae (strain TN)
    Taxonomic identifieri272631 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000806: Chromosome

    Organism-specific databases

    LepromaiML2419.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 329329Delta-aminolevulinic acid dehydratasePRO_0000140504Add
    BLAST

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Protein-protein interaction databases

    STRINGi272631.ML2419.

    Structurei

    3D structure databases

    ProteinModelPortaliP46723.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ALADH family.Curated

    Phylogenomic databases

    eggNOGiCOG0113.
    HOGENOMiHOG000020323.
    KOiK01698.
    OMAiDMILTYF.
    OrthoDBiEOG6VXFCB.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view]
    PANTHERiPTHR11458. PTHR11458. 1 hit.
    PfamiPF00490. ALAD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSiPR00144. DALDHYDRTASE.
    SMARTiSM01004. ALAD. 1 hit.
    [Graphical view]
    PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46723-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVSGYPRHR PRRLRSTPAM RRLVAQTSLE PRNLVLPMFV ADGIDELRPI    50
    ASMPGVVQHT RDSLRRAAVA AVDAGVGGLN LFGVPRDQDK DATGSAGVDP 100
    DGILNVALRD LAEDLGDATV LMADTCLDEF TDHGHCGVLD GQGRVDNDAT 150
    VARYVELAVA QAESGANVVG PSGMMDGQIG ALRDGLDSAG YADVAILAYA 200
    AKFSSAFYGP FREAVSCSLS GDRRTYQQEP GNAREALREI KLDLDEGADI 250
    IMIKPASGYL DVVATAAGVS PVPVAAYQVS GEYAMICAAA ANNWIDERAA 300
    VLESLTSIRR AGADIVFTYW AADVACWLS 329
    Length:329
    Mass (Da):34,775
    Last modified:November 1, 1995 - v1
    Checksum:i1E0EBA9498B8609C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00018 Genomic DNA. Translation: AAA17246.1.
    AL583925 Genomic DNA. Translation: CAC31935.1.
    PIRiS72910.
    RefSeqiNP_302564.1. NC_002677.1.
    WP_010908884.1. NC_002677.1.

    Genome annotation databases

    EnsemblBacteriaiCAC31935; CAC31935; CAC31935.
    GeneIDi908581.
    KEGGimle:ML2419.
    PATRICi18059483. VBIMycLep78757_4656.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00018 Genomic DNA. Translation: AAA17246.1 .
    AL583925 Genomic DNA. Translation: CAC31935.1 .
    PIRi S72910.
    RefSeqi NP_302564.1. NC_002677.1.
    WP_010908884.1. NC_002677.1.

    3D structure databases

    ProteinModelPortali P46723.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272631.ML2419.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC31935 ; CAC31935 ; CAC31935 .
    GeneIDi 908581.
    KEGGi mle:ML2419.
    PATRICi 18059483. VBIMycLep78757_4656.

    Organism-specific databases

    Lepromai ML2419.

    Phylogenomic databases

    eggNOGi COG0113.
    HOGENOMi HOG000020323.
    KOi K01698.
    OMAi DMILTYF.
    OrthoDBi EOG6VXFCB.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00318 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view ]
    PANTHERi PTHR11458. PTHR11458. 1 hit.
    Pfami PF00490. ALAD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSi PR00144. DALDHYDRTASE.
    SMARTi SM01004. ALAD. 1 hit.
    [Graphical view ]
    PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Smith D.R., Robison K.
      Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TN.

    Entry informationi

    Entry nameiHEM2_MYCLE
    AccessioniPrimary (citable) accession number: P46723
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3