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P46715 (BIOB_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:ML1220
ORF Names:B1170_C3_228
OrganismMycobacterium leprae (strain TN) [Complete proteome] [HAMAP]
Taxonomic identifier272631 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length345 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 345345Biotin synthase HAMAP-Rule MF_01694
PRO_0000185559

Sites

Metal binding811Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding851Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding881Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1241Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1571Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2161Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2861Iron-sulfur 2 (2Fe-2S) By similarity

Experimental info

Sequence conflict286 – 2883RFA → PFR in AAA17072. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P46715 [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: C52DC8079BBD0191

FASTA34537,558
        10         20         30         40         50         60 
MTQAGTRSTN GDTENTDILA QARQQVLERG EGLSRDQVLQ MLWLPDDRLE ELLALAHDVR 

        70         80         90        100        110        120 
MRWCGPEVEI EGIISLKTGG CPEDCHFCSQ SGLFMSPVRS AWLDIRSLVE AAKQTAKSGA 

       130        140        150        160        170        180 
TEFCIVAAVR GPDERLMSQV AAGIEAIRNE VEINIACSLG MLTVEQVEQL SGIGVHRYNH 

       190        200        210        220        230        240 
NLETARSFFT NVVTTHTWEE RWQTLSMVRD AGMEVCCGGI LGMGETVEQR AEFALELAEL 

       250        260        270        280        290        300 
GPDEVPLNFL NPRPGTPFGA LEVMPPSEAL KSVAAFRLAL PRTILRFAGG REITLGDLGA 

       310        320        330        340 
KQGMLGGINA VIVGNYLTTL GRPAEADLRL LDDLQMPIKA LNASL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00010 Genomic DNA. Translation: AAA17072.1.
AL583921 Genomic DNA. Translation: CAC31601.1.
PIRF87061.
S72708.
RefSeqNP_301883.1. NC_002677.1.

3D structure databases

ProteinModelPortalP46715.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272631.ML1220.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC31601; CAC31601; CAC31601.
GeneID910327.
KEGGmle:ML1220.
PATRIC18054600. VBIMycLep78757_2240.

Organism-specific databases

LepromaML1220.
CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMANCRFCAQ.
OrthoDBEOG622PMP.
ProtClustDBPRK06256.

Enzyme and pathway databases

UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_MYCLE
AccessionPrimary (citable) accession number: P46715
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: April 27, 2001
Last modified: February 19, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways