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P46715

- BIOB_MYCLE

UniProt

P46715 - BIOB_MYCLE

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Protein

Biotin synthase

Gene

bioB

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi81 – 811Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi85 – 851Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi88 – 881Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi124 – 1241Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi157 – 1571Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi216 – 2161Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi286 – 2861Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:ML1220
ORF Names:B1170_C3_228
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000806: Chromosome

Organism-specific databases

LepromaiML1220.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345Biotin synthasePRO_0000185559Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272631.ML1220.

Structurei

3D structure databases

ProteinModelPortaliP46715.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiDETQALC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

P46715-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQAGTRSTN GDTENTDILA QARQQVLERG EGLSRDQVLQ MLWLPDDRLE
60 70 80 90 100
ELLALAHDVR MRWCGPEVEI EGIISLKTGG CPEDCHFCSQ SGLFMSPVRS
110 120 130 140 150
AWLDIRSLVE AAKQTAKSGA TEFCIVAAVR GPDERLMSQV AAGIEAIRNE
160 170 180 190 200
VEINIACSLG MLTVEQVEQL SGIGVHRYNH NLETARSFFT NVVTTHTWEE
210 220 230 240 250
RWQTLSMVRD AGMEVCCGGI LGMGETVEQR AEFALELAEL GPDEVPLNFL
260 270 280 290 300
NPRPGTPFGA LEVMPPSEAL KSVAAFRLAL PRTILRFAGG REITLGDLGA
310 320 330 340
KQGMLGGINA VIVGNYLTTL GRPAEADLRL LDDLQMPIKA LNASL
Length:345
Mass (Da):37,558
Last modified:April 27, 2001 - v2
Checksum:iC52DC8079BBD0191
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti286 – 2883RFA → PFR in AAA17072. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00010 Genomic DNA. Translation: AAA17072.1.
AL583921 Genomic DNA. Translation: CAC31601.1.
PIRiF87061.
S72708.
RefSeqiNP_301883.1. NC_002677.1.
WP_010908204.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31601; CAC31601; CAC31601.
GeneIDi910327.
KEGGimle:ML1220.
PATRICi18054600. VBIMycLep78757_2240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00010 Genomic DNA. Translation: AAA17072.1 .
AL583921 Genomic DNA. Translation: CAC31601.1 .
PIRi F87061.
S72708.
RefSeqi NP_301883.1. NC_002677.1.
WP_010908204.1. NC_002677.1.

3D structure databases

ProteinModelPortali P46715.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272631.ML1220.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC31601 ; CAC31601 ; CAC31601 .
GeneIDi 910327.
KEGGi mle:ML1220.
PATRICi 18054600. VBIMycLep78757_2240.

Organism-specific databases

Lepromai ML1220.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi DETQALC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Smith D.R., Robison K.
    Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiBIOB_MYCLE
AccessioniPrimary (citable) accession number: P46715
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: April 27, 2001
Last modified: October 1, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3