Skip Header

Contribute Send feedback
Read comments (?) or add your own

P46713 (G3P_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glyceraldehyde-3-phosphate dehydrogenase
Short name=GAPDH
EC=1.2.1.12
Gene names
Name:gapA
Synonyms:gap
Ordered Locus Names:ML0570
ORF Names:B1496_C3_199
OrganismMycobacterium leprae [Complete proteome] [HAMAP]
Taxonomic identifier1769 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length339 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionNAD binding

Inferred from electronic annotation. Source: InterPro

NADP binding

Inferred from electronic annotation. Source: InterPro

glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 339339Glyceraldehyde-3-phosphate dehydrogenase
PRO_0000145669

Regions

Nucleotide binding12 – 132NAD By similarity
Region157 – 1593Glyceraldehyde 3-phosphate binding By similarity
Region216 – 2172Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site1581Nucleophile By similarity
Binding site391NAD By similarity
Binding site841NAD; via carbonyl oxygen By similarity
Binding site1881Glyceraldehyde 3-phosphate By similarity
Binding site2031Glyceraldehyde 3-phosphate By similarity
Binding site2391Glyceraldehyde 3-phosphate By similarity
Binding site3201NAD By similarity
Site1851Activates thiol group during catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
P46713 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 087C477BAADEA5A7

FASTA33936,073
        10         20         30         40         50         60 
MTVRVGINGF GRIGRNFYRA LLAQQEHGIA DVQVVAINDI TDNSTLAYLL KFDSILGRLP 

        70         80         90        100        110        120 
HDVSLEEEDT IVVGSEKIKA LAVREGPAAL PWHAFGVDVV VESTGLFTNA AKAKGHLEAG 

       130        140        150        160        170        180 
AKKVIVSAPA TDPDITIVFG VNDDKYDGSQ NIISNASCTT NCLAPLAKVL HDQFGIVKGL 

       190        200        210        220        230        240 
MTTVHAYTQD QNLQDGPHSD LRRARAAALN VVPTSTGAAK AIGLVMPELK GKLDGYALRV 

       250        260        270        280        290        300 
PIPTGSVTDL TADLSKCVSV NEINAVFQDA AEGRLKGILK YVDAPIVSSD IVTDPHSSIF 

       310        320        330 
DSGLTKVIAS QAKVVSWYDN EWGYSNRLVD LVGLVGKSL

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U00013 Genomic DNA. Translation: AAA17130.1.
AL583919 Genomic DNA. Translation: CAC30078.1.
PIRS72763.
RefSeqNP_301482.1. NC_002677.1.

3D structure databases

ProteinModelPortalP46713.
SMRP46713. Positions 3-337.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000029430; EBMYCP00000029036; EBMYCG00000029425.
GeneID909341.
GenomeReviewsGene locus ML0570 in contig AL450380_GR.
KEGGmle:ML0570.
NMPDRfig|272631.1.peg.354.
PATRIC18052102. VBIMycLep78757_0998.

Organism-specific databases

LepromaML0570.
CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000017630.
HOGENOMHBG571736.
OMAPAKHTVI.
ProtClustDBCLSK791163.

Enzyme and pathway databases

BioCycMLEP272631:ML0570-MONOMER.

Family and domain databases

InterProIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00134.
PANTHERPTHR10836. GAP_DH. 1 hit.
PfamPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000149. GAP_DH. 1 hit.
PRINTSPR00078. G3PDHDRGNASE.
SMARTSM00846. Gp_dh_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01534. GAPDH-I. 1 hit.
PROSITEPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG3P_MYCLE
AccessionPrimary (citable) accession number: P46713
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 25, 2012
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents