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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gapA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.By similarity

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.By similarity

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.Curated
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gapA)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase (eno)
  5. Pyruvate kinase (pykA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391NADBy similarity
Binding sitei84 – 841NAD; via carbonyl oxygenBy similarity
Binding sitei127 – 1271NADBy similarity
Active sitei158 – 1581NucleophileBy similarity
Sitei185 – 1851Activates thiol group during catalysisBy similarity
Binding sitei188 – 1881Glyceraldehyde 3-phosphateBy similarity
Binding sitei203 – 2031Glyceraldehyde 3-phosphateBy similarity
Binding sitei239 – 2391Glyceraldehyde 3-phosphateBy similarity
Binding sitei320 – 3201NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 132NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenaseBy similarity (EC:1.2.1.12By similarity)
Short name:
GAPDHBy similarity
Alternative name(s):
NAD-dependent glyceraldehyde-3-phosphate dehydrogenaseBy similarity
Gene namesi
Name:gapA
Synonyms:gap
Ordered Locus Names:ML0570
ORF Names:B1496_C3_199
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0570.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 339339Glyceraldehyde-3-phosphate dehydrogenasePRO_0000145669Add
BLAST

Proteomic databases

PRIDEiP46713.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi272631.ML0570.

Structurei

3D structure databases

ProteinModelPortaliP46713.
SMRiP46713. Positions 3-337.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni157 – 1593Glyceraldehyde 3-phosphate bindingBy similarity
Regioni216 – 2172Glyceraldehyde 3-phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C17. Bacteria.
COG0057. LUCA.
HOGENOMiHOG000071679.
KOiK00134.
OMAiFVRVLSW.
OrthoDBiPOG091H02BL.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46713-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVRVGINGF GRIGRNFYRA LLAQQEHGIA DVQVVAINDI TDNSTLAYLL
60 70 80 90 100
KFDSILGRLP HDVSLEEEDT IVVGSEKIKA LAVREGPAAL PWHAFGVDVV
110 120 130 140 150
VESTGLFTNA AKAKGHLEAG AKKVIVSAPA TDPDITIVFG VNDDKYDGSQ
160 170 180 190 200
NIISNASCTT NCLAPLAKVL HDQFGIVKGL MTTVHAYTQD QNLQDGPHSD
210 220 230 240 250
LRRARAAALN VVPTSTGAAK AIGLVMPELK GKLDGYALRV PIPTGSVTDL
260 270 280 290 300
TADLSKCVSV NEINAVFQDA AEGRLKGILK YVDAPIVSSD IVTDPHSSIF
310 320 330
DSGLTKVIAS QAKVVSWYDN EWGYSNRLVD LVGLVGKSL
Length:339
Mass (Da):36,073
Last modified:November 1, 1995 - v1
Checksum:i087C477BAADEA5A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00013 Genomic DNA. Translation: AAA17130.1.
AL583919 Genomic DNA. Translation: CAC30078.1.
PIRiS72763.
RefSeqiNP_301482.1. NC_002677.1.
WP_010907806.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC30078; CAC30078; CAC30078.
GeneIDi909341.
KEGGimle:ML0570.
PATRICi18052102. VBIMycLep78757_0998.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00013 Genomic DNA. Translation: AAA17130.1.
AL583919 Genomic DNA. Translation: CAC30078.1.
PIRiS72763.
RefSeqiNP_301482.1. NC_002677.1.
WP_010907806.1. NC_002677.1.

3D structure databases

ProteinModelPortaliP46713.
SMRiP46713. Positions 3-337.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML0570.

Proteomic databases

PRIDEiP46713.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC30078; CAC30078; CAC30078.
GeneIDi909341.
KEGGimle:ML0570.
PATRICi18052102. VBIMycLep78757_0998.

Organism-specific databases

LepromaiML0570.

Phylogenomic databases

eggNOGiENOG4105C17. Bacteria.
COG0057. LUCA.
HOGENOMiHOG000071679.
KOiK00134.
OMAiFVRVLSW.
OrthoDBiPOG091H02BL.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3P_MYCLE
AccessioniPrimary (citable) accession number: P46713
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.