P46708 (TKT_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transketolase
Short name=TK
EC=2.2.1.1

Gene names

Name:	tkt
Ordered Locus Names:	ML0583
ORF Names:	MLCL536.38, B1496_F1_26

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	699 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate.
Cofactor	Binds 1 calcium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Sequence similarities	Belongs to the transketolase family.

Ontologies

Keywords
Ligand	Calcium Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW transketolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 699	699	Transketolase		PRO_0000191861
Sites
	Metal binding	177	1	Calcium By similarity
	Metal binding	207	1	Calcium By similarity
	Metal binding	209	1	Calcium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46708 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 64900CFB9B4A4481	FASTA	699	75,642
	10 20 30 40 50 60 MTTLDQISTL TQPRHPDDWT EIDSAAVDTI RVLATDAVQK AGNGHPGTAM SLAPLAYTLF 70 80 90 100 110 120 QRTLRHDPND TAWLGRDRFV LSAGHSSLTL YIQLYLGGFG LELSDIESLR TWGSTTPGHP 130 140 150 160 170 180 EFRHTKGVEI TTGPLGQGLA SAVGMAMASR YERGLFDPDA EPGASPFDHY IYVIASDGDI 190 200 210 220 230 240 EEGVTSEASS LAAVQQLGNL IVFYDHNQIS IEGDTKITLC EDTAARYRAY GWHVQEVEGG 250 260 270 280 290 300 ENVVGIEEAI ANAKAATDRP SFISLRTIIG YPAPTLINTG KAHGAALGED EVAATKRILG 310 320 330 340 350 360 FDPDKTFAVR EDVITHTRGL IARGKEAHER WQLEFEAWAQ REPERKALLD RLLAQQLPDG 370 380 390 400 410 420 WDADLPNWEP RSKELATRAA SGAVLSAIGP KLPELWGGSA DLAGSNNTTI KDVDSFGPPS 430 440 450 460 470 480 ISTDEYTAHW YGRTLHFGVR EHAMGAILSG IVLHGPTRAY GGTFLQFSDY MRPSVRLASL 490 500 510 520 530 540 MDIDTIYVWT HDSVGLGEDG PTHQPIEHLA ALRAIPRLSV VRPADANETA YAWRTILARG 550 560 570 580 590 600 ANSGPVGLIL TRQSVPVLEG TNTEGVARGG YVLGDGGSSE AKEPDVILIA TGSEVQLAVA 610 620 630 640 650 660 AQKLLADKDI IVRVVSMPCV EWFESQPYEY RDSVLPPSVS ARVAVEAGVA QCWHKLVGDT 670 680 690 GKIVSIEHYG ESADYQTLFR EYGFTPEAVV AAAEQVLDN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Smith D.R., Robison K. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00013 Genomic DNA. Translation: AAA17139.1. Z99125 Genomic DNA. Translation: CAB16182.1. AL583919 Genomic DNA. Translation: CAC30091.1.
PIR	S72772.
RefSeq	NP_301494.1. NC_002677.1.
3D structure databases
ProteinModelPortal	P46708.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000029009; EBMYCP00000028615; EBMYCG00000029004.
GeneID	910090.
GenomeReviews	Gene locus ML0583 in contig AL450380_GR.
KEGG	mle:ML0583.
NMPDR	fig|272631.1.peg.366.
PATRIC	18052140. VBIMycLep78757_1017.
Organism-specific databases
Leproma	ML0583.
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016270.
HOGENOM	HBG565885.
OMA	WPLEPEF.
ProtClustDB	PRK05899.
Enzyme and pathway databases
BioCyc	MLEP272631:ML0583-MONOMER.
Family and domain databases
InterPro	IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005478. Transketolase_bac-like. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K00615.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. PF00456. Transketolase_N. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00232. Tktlase_bact. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TKT_MYCLE

Accession

Primary (citable) accession number: P46708

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program