Reviewed, UniProtKB/Swiss-Prot P46701 (PURK_MYCLE)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase ATPase subunit
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:	purK
Ordered Locus Names:	ML0735
ORF Names:	B1308_F1_32

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses an ATPase activity that is dependent on the presence of AIR (aminoimidazole ribonucleotide). The association of purK and purE produces an enzyme complex capable of converting AIR to CAIR efficiently under physiological condition By similarity.
Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purK/purT family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	Phosphoribosylaminoimidazole carboxylase ATPase subunit		PRO_0000074999
Regions
	Domain	117 – 313	197	ATP-grasp
	Nucleotide binding	197 – 200	4	ATP By similarity
	Nucleotide binding	283 – 284	2	ATP By similarity
Sites
	Binding site	113	1	ATP By similarity
	Binding site	160	1	ATP By similarity
	Binding site	205	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46701-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 288C5DD5EE7508EE	FASTA	439	47,306
	10 20 30 40 50 60 MMAVPSRCSL GVAPLVAMVG GGQLARMTHQ AAIALGQTLR VLATAADEPA AQVTPDVVIG 70 80 90 100 110 120 SHTDLEDLRR VALGADALTF DHEHVPTELL DKLVAEGINV APSPQALVHA QDKLVMRRRL 130 140 150 160 170 180 AALGAAMPRF MALDSVDDLA EIDAFAQRLT GSKDAPMVVK AVRGGYDGRG VQMVRDSAHA 190 200 210 220 230 240 REVASGYLVD GMPVLVEERV ELRRELSALV ARSPFGQGAA WPVVETVQRD GICVLVVAPA 250 260 270 280 290 300 LALADDLASA AQQLALRLAA ELGVVGVFAV ELFETADGAL LVNELAMRPH NSGHWTMDGA 310 320 330 340 350 360 RTSQFEQHLR AVLDYPLGET DAVAPVTVMV NVLGAPQPPT LSVVTMDERL HHLFARMPDA 370 380 390 400 410 420 RVHLYDKVER PGRKVGHINF RGTDKDRKNP TKLRERAELA AHWLSHGQWT DGWDPHRAGD 430 DVVEISLACG GRNDAQRQR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic organization of the mycobacterial sigma gene cluster." Doukhan L., Predich M., Nair G., Dussurget O., Mandic-Mulec I., Cole S.T., Smith D.R., Smith I. Gene 165:67-70(1995) [PubMed: 7489918] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00012 Genomic DNA. Translation: AAA85935.1. AL583919 Genomic DNA. Translation: CAC30244.1.
PIR	H87000.
RefSeq	NP_301575.1.
3D structure databases
HSSP	HSSP built from PDB template 1B6R based on UniProtKB P09029.
ModBase	Search...
Genome annotation databases
GeneID	909683.
GenomeReviews	Gene locus ML0735 in contig AL450380_GR.
KEGG	mle:ML0735.
NMPDR	fig|272631.1.peg.447.
Organism-specific databases
Leproma	ML0735.
CMR	Search...
Phylogenomic databases
HOGENOM	P46701.
OMA	RKIGHVN.
Enzyme and pathway databases
BioCyc	MLEP272631:ML0735-MON.
BRENDA	4.1.1.21. 808.
Family and domain databases
InterPro	IPR005875. AIR_COase_ATPase-su. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PANTHER	PTHR23047:SF1. PurK_ATP. 1 hit.
Pfam	PF02222. ATP-grasp. 1 hit. [Graphical view]
TIGRFAMs	TIGR01161. purK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURK_MYCLE

Accession

Primary (citable) accession number: P46701

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)