Reviewed, UniProtKB/Swiss-Prot P46695 (IEX1_HUMAN)

Names and origin

Protein names

Recommended name:
    Radiation-inducible immediate-early gene IEX-1
Alternative name(s):
    Immediate early protein GLY96
    Immediate early response 3 protein
    PACAP-responsive gene 1 protein
      Short name=Protein PRG1
    Differentiation-dependent gene 2 protein
      Short name=Protein DIF-2

Gene names

Name:	IER3
Synonyms:	DIF2, IEX1, PRG1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Membrane; Single-pass type II membrane protein Potential.
Induction	By radiation, TPA, okadaic acid and TNF-alpha.
Post-translational modification	Glycosylated.
Sequence similarities	Belongs to the IER3 family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	anatomical structure morphogenesis Ref.3 Traceable author statement. Source: ProtInc anti-apoptosis Traceable author statement. Source: ProtInc apoptosis Ref.3 Traceable author statement. Source: ProtInc
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAMLG	P49069	1	EBI-1748945,EBI-1748958

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 156	156	Radiation-inducible immediate-early gene IEX-1		PRO_0000084159
Regions
	Topological domain	1 – 82	82	Cytoplasmic Potential
	Transmembrane	83 – 99	17	Signal-anchor for type II membrane protein Potential
	Topological domain	100 – 156	57	Extracellular Potential
Amino acid modifications
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	127	1	P → A: dbSNP rs3094124. Ref.6		VAR_058496
Experimental info
	Sequence conflict	54	1	A → G in AAB36278. Ref.1
	Sequence conflict	106	1	P → R in AAB36278. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P46695-1 [UniParc]. Last modified July 15, 1998. Version 3. Checksum: 83C06116C80B9240	FASTA	156	16,929
	10 20 30 40 50 60 MCHSRSCHPT MTILQAPTPA PSTIPGPRRG SGPEIFTFDP LPEPAAAPAG RPSASRGHRK 70 80 90 100 110 120 RSRRVLYPRV VRRQLPVEEP NPAKRLLFLL LTIVFCQILM AEEGVPAPLP PEDAPNAASL 130 140 150 APTPVSPVLE PFNLTSEPSD YALDLSTFLQ QHPAAF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a radiation-inducible glycosylated human early-response gene." Kondratyev A.D., Chung K.-N., Jung M.O. Cancer Res. 56:1498-1502(1996) [PubMed: 8603392] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[2]	"PRG1: a novel early-response gene transcriptionally induced by pituitary adenylate cyclase activating polypeptide in a pancreatic carcinoma cell line." Schaefer H., Trauzold A., Siegel E.G., Folsch U.R., Schmidt W.E. Cancer Res. 56:2641-2648(1996) [PubMed: 8653710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Identification and characterization of a novel monocyte/macrophage differentiation-dependent gene that is responsive to lipopolysaccharide, ceramide, and lysophosphatidylcholine." Pietzsch A., Buechler C., Aslanidis C., Schmitz G. Biochem. Biophys. Res. Commun. 235:4-9(1997) [PubMed: 9196025] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Homo sapiens 2,229,817bp genomic DNA of 6p21.3 HLA class I region." Shiina S., Tamiya G., Oka A., Inoko H. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ALA-127.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cervix and Skin.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S81914 mRNA. Translation: AAB36278.1. X96438 Genomic DNA. Translation: CAA65304.1. Y14551 mRNA. Translation: CAA74886.1. BT006703 mRNA. Translation: AAP35349.1. BA000025 Genomic DNA. Translation: BAB63319.1. AL662797 Genomic DNA. Translation: CAI18210.1. BC000844 mRNA. Translation: AAH00844.1. BC005080 mRNA. Translation: AAH05080.1.
IPI	IPI00007831.
PIR	JC5537.
RefSeq	NP_003888.2.
UniGene	Hs.591785
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P46695. 4 interactions.
STRING	P46695.
PTM databases
PhosphoSite	P46695.
Proteomic databases
PRIDE	P46695.
Genome annotation databases
Ensembl	ENST00000383560; ENSP00000373054; ENSG00000206478; Homo sapiens. [Genome view] ENST00000400509; ENSP00000383354; ENSG00000206478; Homo sapiens. [Genome view] ENST00000416336; ENSP00000411106; ENSG00000237155; Homo sapiens. [Genome view] ENST00000416665; ENSP00000397919; ENSG00000230128; Homo sapiens. [Genome view] ENST00000416884; ENSP00000406245; ENSG00000227231; Homo sapiens. [Genome view] ENST00000425070; ENSP00000409310; ENSG00000227231; Homo sapiens. [Genome view] ENST00000427437; ENSP00000397934; ENSG00000237155; Homo sapiens. [Genome view] ENST00000429592; ENSP00000398052; ENSG00000227231; Homo sapiens. [Genome view] ENST00000435856; ENSP00000412283; ENSG00000235030; Homo sapiens. [Genome view] ENST00000436988; ENSP00000412861; ENSG00000230128; Homo sapiens. [Genome view] ENST00000439190; ENSP00000397956; ENSG00000237155; Homo sapiens. [Genome view] ENST00000439580; ENSP00000393940; ENSG00000206478; Homo sapiens. [Genome view] ENST00000446599; ENSP00000394148; ENSG00000235030; Homo sapiens. [Genome view] ENST00000450236; ENSP00000398139; ENSG00000230128; Homo sapiens. [Genome view] ENST00000451459; ENSP00000404555; ENSG00000235030; Homo sapiens. [Genome view]
GeneID	8870.
KEGG	hsa:8870.
Organism-specific databases
CTD	8870.
GeneCards	GC06M030818. GC06Mc30848. GC06Mf30843.
H-InvDB	HIX0005703. HIX0057742. HIX0058162.
HGNC	HGNC:5392. IER3.
HPA	CAB019300.
MIM	602996. gene.
PharmGKB	PA29639.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	P46695.
Gene expression databases
CleanEx	HS_IER3.
Genevestigator	P46695.
GermOnline	ENSG00000137331. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	33301.
SOURCE	Search...

Entry information

Entry name

IEX1_HUMAN

Accession

Primary (citable) accession number: P46695
Secondary accession number(s): Q5SU30, Q92691, Q93044

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	July 15, 1998
Last modified:	October 13, 2009
This is version 79 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.