Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P46694 (IEX1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Radiation-inducible immediate-early gene IEX-1
Alternative name(s):
Immediate early protein GLY96
Immediate early response 3 protein
Gene names
Name:Ier3
Synonyms:Gly96, Iex1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length153 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival By similarity. As a member of the NUPR1/RELB/IER3 survival pathway, may allow the development of pancreatic intraepithelial neoplasias. Ref.2

Subunit structure

Interacts with the PPP2R5C-PP2A holoenzyme and ERK kinases; regulates ERK dephosphorylation By similarity.

Subcellular location

Membrane; Single-pass type II membrane protein By similarity.

Tissue specificity

Expressed predominantly in the lung, testes and the uterus.

Induction

By serum growth factors and TPA.

Post-translational modification

Glycosylated.

Sequence similarities

Belongs to the IER3 family.

Ontologies

Keywords
   Cellular componentMembrane
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processintrinsic apoptotic signaling pathway in response to DNA damage

Inferred from direct assay PubMed 11753682. Source: MGI

mitotic G2 DNA damage checkpoint

Inferred from mutant phenotype PubMed 20467439. Source: MGI

negative regulation of apoptotic process

Inferred from electronic annotation. Source: InterPro

negative regulation of glycolytic process

Inferred from mutant phenotype PubMed 19096392. Source: MGI

negative regulation of inflammatory response

Inferred from mutant phenotype PubMed 19923449. Source: MGI

negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway

Inferred from direct assay PubMed 19096392. Source: MGI

negative regulation of systemic arterial blood pressure

Inferred from mutant phenotype PubMed 16166241. Source: MGI

positive regulation of protein catabolic process

Inferred from direct assay PubMed 19096392. Source: MGI

regulation of DNA repair

Inferred from mutant phenotype PubMed 20467439. Source: MGI

regulation of nucleocytoplasmic transport

Inferred from mutant phenotype PubMed 20467439. Source: MGI

regulation of reactive oxygen species metabolic process

Inferred from direct assay PubMed 19096392. Source: MGI

regulation of response to DNA damage stimulus

Inferred from mutant phenotype PubMed 20467439. Source: MGI

response to protozoan

Inferred from mutant phenotype PubMed 19923449. Source: MGI

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

nucleus

Inferred from direct assay PubMed 20467439. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 153153Radiation-inducible immediate-early gene IEX-1
PRO_0000084160

Regions

Topological domain1 – 8585Cytoplasmic Potential
Transmembrane86 – 10217Helical; Signal-anchor for type II membrane protein; Potential
Topological domain103 – 15351Extracellular Potential

Amino acid modifications

Modified residue311Phosphoserine By similarity
Glycosylation1371N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P46694 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: F4429E3B0120DE11

FASTA15316,875
        10         20         30         40         50         60 
MCHSRNHLHT MTGLRAPSPA PSTGPELRRG SGPEIFTFDP LPERAVVSTA RLNTSRGHRK 

        70         80         90        100        110        120 
RSRRVLYPRV VRRQLPTEEP NIAKRVLFLL FAIIFCQILM AEEGVSQPLA PEDATSAVTP 

       130        140        150 
EPISAPITAP PVLEPLNLTS ESSDYALDLK AFL 

« Hide

References

[1]"Genomic structure, cDNA sequence, and expression of gly96, a growth factor-inducible immediate-early gene encoding a short-lived glycosylated protein."
Charles C.H., Yoon J.K., Simske J.S., Lau L.F.
Oncogene 8:797-801(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[2]"Nuclear protein 1 promotes pancreatic cancer development and protects cells from stress by inhibiting apoptosis."
Hamidi T., Algul H., Cano C.E., Sandi M.J., Molejon M.I., Riemann M., Calvo E.L., Lomberk G., Dagorn J.C., Weih F., Urrutia R., Schmid R.M., Iovanna J.L.
J. Clin. Invest. 122:2092-2103(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X67644 mRNA. No translation available.
PIRS33363.
UniGeneMm.25613.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000003635.

PTM databases

PhosphoSiteP46694.

Proteomic databases

PRIDEP46694.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:104814. Ier3.

Phylogenomic databases

eggNOGNOG46208.
HOVERGENHBG000172.
InParanoidP46694.
PhylomeDBP46694.

Gene expression databases

ArrayExpressP46694.
BgeeP46694.
CleanExMM_IER3.
GenevestigatorP46694.

Family and domain databases

InterProIPR024829. IEX-1.
[Graphical view]
PANTHERPTHR16915. PTHR16915. 1 hit.
PRINTSPR02100. GENEIEX1.
ProtoNetSearch...

Other

ChiTaRSIER3. mouse.
PROP46694.
SOURCESearch...

Entry information

Entry nameIEX1_MOUSE
AccessionPrimary (citable) accession number: P46694
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot