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Protein

Zinc finger protein ZIC 1

Gene

Zic1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally-located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'.7 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri225 – 26036C2H2-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri269 – 29628C2H2-type 2; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri302 – 32625C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri332 – 35625C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri362 – 38423C2H2-type 5PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • adult walking behavior Source: MGI
  • brain development Source: UniProtKB
  • cell differentiation Source: UniProtKB-KW
  • central nervous system development Source: MGI
  • inner ear morphogenesis Source: UniProtKB
  • pattern specification process Source: UniProtKB
  • positive regulation of protein import into nucleus Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • regulation of smoothened signaling pathway Source: UniProtKB
  • skeletal system development Source: UniProtKB
  • spinal cord development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein ZIC 1
Alternative name(s):
Zinc finger protein of the cerebellum 1
Gene namesi
Name:Zic1
Synonyms:Zic
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:106683. Zic1.

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Localizes in the cytoplasm in presence of MDFIC overexpression.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice show a cell mass decrease of the spinal dorsal horn, hypoplasia and abnormal foliation patterns in the cerebellum.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447Zinc finger protein ZIC 1PRO_0000047245Add
BLAST

Proteomic databases

MaxQBiP46684.
PaxDbiP46684.
PeptideAtlasiP46684.
PRIDEiP46684.

PTM databases

iPTMnetiP46684.

Expressioni

Tissue specificityi

Expressed in osteoblasts (at protein level). Expressed in the CNS. A high level expression is seen in the cerebellum, while a low level expression is seen in the olfactory bulb, diencephalon, and brainstem. Expressed in lumbar spine and iliac crest.3 Publications

Developmental stagei

Expressed in progenitor cells in the dorsal third of the ventricular zone at 12.5 dpc. Expressed in newly emerging pontine gray nucleus (PGN) precursor cells of the extramural migratory stream (ems) between 12.5 and 14.5 dpc. Expressed in precerebellar mossy fiber (MF) neurons of the PGN (located either rostromedially or caudolaterally) persisted through at least P8 after birth (at protein level). In the early embryonic stage, it is expressed in the dorsal half of the neural tube and adjacent mesenchyme, and in the developing cerebellum it is expressed persistently in the granule cell lineage throughout the prenatal and postnatal periods.2 Publications

Gene expression databases

BgeeiP46684.
CleanExiMM_ZIC1.
GenevisibleiP46684. MM.

Interactioni

Subunit structurei

Interacts (via the C2H2-type domains 3, 4 and 5) with MDFIC (via the C2H2-type domains 3, 4 and 5). Interacts with GLI1; the interaction enhances transcription activation. Interacts with GLI2. Interacts with GLI3; the interaction enhances transcription activation.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GLI1P081512EBI-308006,EBI-308084From a different organism.
Gli2Q8K0K32EBI-308006,EBI-308039
GLI3P100712EBI-308006,EBI-308055From a different organism.

Protein-protein interaction databases

BioGridi204694. 1 interaction.
IntActiP46684. 3 interactions.
MINTiMINT-189851.
STRINGi10090.ENSMUSP00000034927.

Structurei

3D structure databases

ProteinModelPortaliP46684.
SMRiP46684. Positions 219-386.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi73 – 764Poly-Ala
Compositional biasi79 – 824Poly-His
Compositional biasi110 – 1167Poly-Ala
Compositional biasi386 – 44055Ser-richAdd
BLAST

Domaini

The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.

Sequence similaritiesi

Contains 5 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri225 – 26036C2H2-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri269 – 29628C2H2-type 2; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri302 – 32625C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri332 – 35625C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri362 – 38423C2H2-type 5PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000118771.
HOGENOMiHOG000232057.
HOVERGENiHBG007135.
InParanoidiP46684.
KOiK09224.
OMAiNMNMAAH.
OrthoDBiEOG76472R.
TreeFamiTF351425.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 2 hits.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46684-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLDAGPQYP AIGVTTFGAS RHHSAGDVAE RDVGLGINPF ADGMGAFKLN
60 70 80 90 100
PSSHELASAG QTAFTSQAPG YAAAAALGHH HHPGHVGSYS SAAFNSTRDF
110 120 130 140 150
LFRNRGFGDA AAAASAQHSL FAASAGGFGG PHGHTDAAGH LLFSGLHEQA
160 170 180 190 200
AGHASPNVVN GQMRLGFSGD MYPRPEQYGQ VTSPRSEHYA APQLHGYGPM
210 220 230 240 250
NVNMAAHHGA GAFFRYMRQP IKQELICKWI EPEQLANPKK SCNKTFSTMH
260 270 280 290 300
ELVTHVTVEH VGGPEQSNHI CFWEECPREG KPFKAKYKLV NHIRVHTGEK
310 320 330 340 350
PFPCPFPGCG KVFARSENLK IHKRTHTGEK PFKCEFEGCD RRFANSSDRK
360 370 380 390 400
KHMHVHTSDK PYLCKMCDKS YTHPSSLRKH MKVHESSSQG SQPSPAASSG
410 420 430 440
YESSTPPTIV SPTTDNPTTS SMSPSSSAVH HTAGHSALSS NFNEWYV
Length:447
Mass (Da):48,331
Last modified:July 27, 2011 - v2
Checksum:iD41F412F44C2AD3D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 721A → R in BAA06878 (PubMed:7931345).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32167 mRNA. Translation: BAA06878.1.
AK136977 mRNA. Translation: BAE23194.1.
CH466560 Genomic DNA. Translation: EDL20910.1.
BC050889 mRNA. Translation: AAH50889.2.
BC060247 mRNA. Translation: AAH60247.1.
BC063247 mRNA. Translation: AAH63247.1.
CCDSiCCDS23403.1.
PIRiI56511.
RefSeqiNP_033599.2. NM_009573.3.
XP_006511124.1. XM_006511061.1.
UniGeneiMm.335350.
Mm.477226.

Genome annotation databases

EnsembliENSMUST00000034927; ENSMUSP00000034927; ENSMUSG00000032368.
ENSMUST00000065360; ENSMUSP00000068858; ENSMUSG00000032368.
GeneIDi22771.
KEGGimmu:22771.
UCSCiuc009raf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32167 mRNA. Translation: BAA06878.1.
AK136977 mRNA. Translation: BAE23194.1.
CH466560 Genomic DNA. Translation: EDL20910.1.
BC050889 mRNA. Translation: AAH50889.2.
BC060247 mRNA. Translation: AAH60247.1.
BC063247 mRNA. Translation: AAH63247.1.
CCDSiCCDS23403.1.
PIRiI56511.
RefSeqiNP_033599.2. NM_009573.3.
XP_006511124.1. XM_006511061.1.
UniGeneiMm.335350.
Mm.477226.

3D structure databases

ProteinModelPortaliP46684.
SMRiP46684. Positions 219-386.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204694. 1 interaction.
IntActiP46684. 3 interactions.
MINTiMINT-189851.
STRINGi10090.ENSMUSP00000034927.

PTM databases

iPTMnetiP46684.

Proteomic databases

MaxQBiP46684.
PaxDbiP46684.
PeptideAtlasiP46684.
PRIDEiP46684.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034927; ENSMUSP00000034927; ENSMUSG00000032368.
ENSMUST00000065360; ENSMUSP00000068858; ENSMUSG00000032368.
GeneIDi22771.
KEGGimmu:22771.
UCSCiuc009raf.1. mouse.

Organism-specific databases

CTDi7545.
MGIiMGI:106683. Zic1.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000118771.
HOGENOMiHOG000232057.
HOVERGENiHBG007135.
InParanoidiP46684.
KOiK09224.
OMAiNMNMAAH.
OrthoDBiEOG76472R.
TreeFamiTF351425.

Miscellaneous databases

PROiP46684.
SOURCEiSearch...

Gene expression databases

BgeeiP46684.
CleanExiMM_ZIC1.
GenevisibleiP46684. MM.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 2 hits.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel zinc finger protein, zic, is involved in neurogenesis, especially in the cell lineage of cerebellar granule cells."
    Aruga J., Yokota N., Hashimoto M., Furuichi T., Fukuda M., Mikoshiba K.
    J. Neurochem. 63:1880-1890(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: ICR.
    Tissue: Cerebellum.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  5. "Molecular properties of Zic proteins as transcriptional regulators and their relationship to GLI proteins."
    Mizugishi K., Aruga J., Nakata K., Mikoshiba K.
    J. Biol. Chem. 276:2180-2188(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING.
  6. "Physical and functional interactions between Zic and Gli proteins."
    Koyabu Y., Nakata K., Mizugishi K., Aruga J., Mikoshiba K.
    J. Biol. Chem. 276:6889-6892(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GLI1; GLI2 AND GLI3, SUBCELLULAR LOCATION.
  7. "Zic1 promotes the expansion of dorsal neural progenitors in spinal cord by inhibiting neuronal differentiation."
    Aruga J., Tohmonda T., Homma S., Mikoshiba K.
    Dev. Biol. 244:329-341(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
  8. "Myogenic repressor I-mfa interferes with the function of Zic family proteins."
    Mizugishi K., Hatayama M., Tohmonda T., Ogawa M., Inoue T., Mikoshiba K., Aruga J.
    Biochem. Biophys. Res. Commun. 320:233-240(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MDFIC, SUBCELLULAR LOCATION.
  9. "Zic1 levels regulate mossy fiber neuron position and axon laterality choice in the ventral brain stem."
    Dipietrantonio H.J., Dymecki S.M.
    Neuroscience 162:560-573(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  10. "Zic1 transcription factor in bone: neural developmental protein regulates mechanotransduction in osteocytes."
    Kalogeropoulos M., Varanasi S.S., Olstad O.K., Sanderson P., Gautvik V.T., Reppe S., Francis R.M., Gautvik K.M., Birch M.A., Datta H.K.
    FASEB J. 24:2893-2903(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiZIC1_MOUSE
AccessioniPrimary (citable) accession number: P46684
Secondary accession number(s): Q6PAK5, Q80Y18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.