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Protein

AP-3 complex subunit beta

Gene

APL6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to the vacuole. Required for the transport via the ALP pathway, which directs the transport of the cargo proteins PHO8 and VAM3 to the vacuole.3 Publications

Miscellaneous

Present with 3570 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • Golgi to vacuole transport Source: SGD
  • protein targeting to vacuole Source: SGD

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30930-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-3 complex subunit beta
Alternative name(s):
Adaptor-related protein complex 3 subunit beta
Beta-3-adaptin
Clathrin assembly protein complex 3 beta large chain
Clathrin assembly protein large beta chain
Gene namesi
Name:APL6
Synonyms:YKS5
Ordered Locus Names:YGR261C
ORF Names:G9331
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR261C.
SGDiS000003493. APL6.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001937571 – 809AP-3 complex subunit betaAdd BLAST809

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei693PhosphoserineCombined sources1
Modified residuei698PhosphoserineCombined sources1
Modified residuei724PhosphoserineCombined sources1
Modified residuei726PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP46682.
PRIDEiP46682.

PTM databases

iPTMnetiP46682.

Interactioni

Subunit structurei

Adaptor protein complex 3 (AP-3) is a heterotetramer composed of 2 large adaptins (APL5 and APL6), a medium adaptin (APM3) and a small adaptin (APS3).

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33512. 231 interactors.
DIPiDIP-2684N.
IntActiP46682. 26 interactors.
MINTiMINT-530434.
STRINGi4932.YGR261C.

Structurei

3D structure databases

ProteinModelPortaliP46682.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati37 – 76HEAT 1Add BLAST40
Repeati112 – 151HEAT 2Add BLAST40
Repeati153 – 186HEAT 3Add BLAST34
Repeati187 – 224HEAT 4Add BLAST38
Repeati524 – 561HEAT 5Add BLAST38

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi771 – 789Asp/Glu-rich (acidic)Add BLAST19

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00530000063546.
HOGENOMiHOG000248160.
InParanoidiP46682.
KOiK12397.
OMAiQSVVLNC.
OrthoDBiEOG092C18N0.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiView protein in InterPro
IPR026740. AP3_beta.
IPR026739. AP_beta.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
PANTHERiPTHR11134. PTHR11134. 1 hit.
PTHR11134:SF20. PTHR11134:SF20. 1 hit.
PfamiView protein in Pfam
PF01602. Adaptin_N. 1 hit.
SUPFAMiSSF48371. SSF48371. 2 hits.

Sequencei

Sequence statusi: Complete.

P46682-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDSIHRIAS ALDTAKVITR EAAAVATSKL GESSYTYYSQ NINPQQLVTL
60 70 80 90 100
LNSRNSREVR DAMKRIISIM ASDDDSIDVQ LYFADVVKNI TTNDTKVKRL
110 120 130 140 150
IHLYLLRFAE NDPNLTLLSI NSLQKSLSDS NSELRCFALS ALSDMKMSSL
160 170 180 190 200
APIILHTVKK LVTDPSAMVR GEVALAIIKL YRAGKNDYHE ELLDILKELM
210 220 230 240 250
ADTDPKVISC AVLAYKECYA DHLELLHGHF RRYCRIIKQL DSWSQSYLIE
260 270 280 290 300
LLIKYCKQYL PKPTVVDKSS EGSPRSCPLP DKYNEIEYPS YEVVNDPDLD
310 320 330 340 350
LFLQSLNCLI YSSNPTVILS CCNALYQLAS PLQMKNTKFI EALVRTVTMT
360 370 380 390 400
ENQGNKEMLL QAIHFLSILD QTLFLPYTKK FYVFPKDPIV ASIWKIQILS
410 420 430 440 450
TLINESNVKE IFKELKYYVA SAHFPENVVI MAVKSLSRCG QLSTSWESHV
460 470 480 490 500
MKWLIDHMES HNLSASVLDA YVNVIRMLVQ KNPTKHLRII FKLADLLTVQ
510 520 530 540 550
TSLADNARAG IVWLFGEIAS IEFKICPDVL RRLIQNFSNE GPETRCQILV
560 570 580 590 600
LSAKLLSYDI DNFKQAQVTG SEENNQNPPY YDFSGSRISQ MYNAVLYLAK
610 620 630 640 650
YDDEFDIRDR ARMISSLFDS GKYEIVSLLL QAPKPTARSD DFIVSARLET
660 670 680 690 700
HTPEIKEFFR MLPWNTEITE VGETGNDIRE GAELKDYNKY KKSFSSQSFI
710 720 730 740 750
TNNSARSFTS SSNAKLTGIN DGDSNSISGK GNVNTFTSQN GKKYRLQSLD
760 770 780 790 800
EFFSDIPERK SKPRKIIKVV EESSDEDEDE SEESSDDDEY SDSSLGTSSS

GTSSSHLEL
Length:809
Mass (Da):91,607
Last modified:October 1, 1996 - v2
Checksum:i475DA3A4F6E68E97
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27 – 35TSKLGESSY → PLSWVNPP in AAC13877 (Ref. 1) Curated9
Sequence conflicti724S → T in AAC13877 (Ref. 1) Curated1
Sequence conflicti798 – 802Missing in AAC13877 (Ref. 1) Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35411 Genomic DNA. Translation: AAC13877.1.
Y07777 Genomic DNA. Translation: CAA69083.1.
Z73046 Genomic DNA. Translation: CAA97290.1.
BK006941 Genomic DNA. Translation: DAA08351.1.
PIRiS64594.
RefSeqiNP_011777.3. NM_001181390.3.

Genome annotation databases

EnsemblFungiiYGR261C; YGR261C; YGR261C.
GeneIDi853177.
KEGGisce:YGR261C.

Similar proteinsi

Entry informationi

Entry nameiAP3B_YEAST
AccessioniPrimary (citable) accession number: P46682
Secondary accession number(s): D6VV40
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: September 27, 2017
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names