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Protein

AP-3 complex subunit beta

Gene

APL6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to the vacuole. Required for the transport via the ALP pathway, which directs the transport of the cargo proteins PHO8 and VAM3 to the vacuole.3 Publications

GO - Biological processi

  • Golgi to vacuole transport Source: SGD
  • protein targeting to vacuole Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30930-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-3 complex subunit beta
Alternative name(s):
Adaptor-related protein complex 3 subunit beta
Beta-3-adaptin
Clathrin assembly protein complex 3 beta large chain
Clathrin assembly protein large beta chain
Gene namesi
Name:APL6
Synonyms:YKS5
Ordered Locus Names:YGR261C
ORF Names:G9331
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR261C.
SGDiS000003493. APL6.

Subcellular locationi

GO - Cellular componenti

  • AP-3 adaptor complex Source: SGD
  • clathrin-coated vesicle membrane Source: UniProtKB-SubCell
  • Golgi apparatus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 809809AP-3 complex subunit betaPRO_0000193757Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei693 – 6931PhosphoserineCombined sources
Modified residuei698 – 6981PhosphoserineCombined sources
Modified residuei724 – 7241PhosphoserineCombined sources
Modified residuei726 – 7261PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP46682.
PeptideAtlasiP46682.

PTM databases

iPTMnetiP46682.

Interactioni

Subunit structurei

Adaptor protein complex 3 (AP-3) is a heterotetramer composed of 2 large adaptins (APL5 and APL6), a medium adaptin (APM3) and a small adaptin (APS3).

Binary interactionsi

WithEntry#Exp.IntActNotes
APL5Q089515EBI-2213,EBI-29702
APM3P381535EBI-2213,EBI-2710

Protein-protein interaction databases

BioGridi33512. 97 interactions.
DIPiDIP-2684N.
IntActiP46682. 16 interactions.
MINTiMINT-530434.

Structurei

3D structure databases

ProteinModelPortaliP46682.
SMRiP46682. Positions 37-582.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati37 – 7640HEAT 1Add
BLAST
Repeati112 – 15140HEAT 2Add
BLAST
Repeati153 – 18634HEAT 3Add
BLAST
Repeati187 – 22438HEAT 4Add
BLAST
Repeati524 – 56138HEAT 5Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi771 – 78919Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Contains 5 HEAT repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00530000063546.
HOGENOMiHOG000248160.
InParanoidiP46682.
OMAiRKLVYIF.
OrthoDBiEOG74TX76.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR026740. AP3_beta.
IPR026739. AP_beta.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
[Graphical view]
PANTHERiPTHR11134. PTHR11134. 4 hits.
PTHR11134:SF1. PTHR11134:SF1. 4 hits.
PfamiPF01602. Adaptin_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.

Sequencei

Sequence statusi: Complete.

P46682-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDSIHRIAS ALDTAKVITR EAAAVATSKL GESSYTYYSQ NINPQQLVTL
60 70 80 90 100
LNSRNSREVR DAMKRIISIM ASDDDSIDVQ LYFADVVKNI TTNDTKVKRL
110 120 130 140 150
IHLYLLRFAE NDPNLTLLSI NSLQKSLSDS NSELRCFALS ALSDMKMSSL
160 170 180 190 200
APIILHTVKK LVTDPSAMVR GEVALAIIKL YRAGKNDYHE ELLDILKELM
210 220 230 240 250
ADTDPKVISC AVLAYKECYA DHLELLHGHF RRYCRIIKQL DSWSQSYLIE
260 270 280 290 300
LLIKYCKQYL PKPTVVDKSS EGSPRSCPLP DKYNEIEYPS YEVVNDPDLD
310 320 330 340 350
LFLQSLNCLI YSSNPTVILS CCNALYQLAS PLQMKNTKFI EALVRTVTMT
360 370 380 390 400
ENQGNKEMLL QAIHFLSILD QTLFLPYTKK FYVFPKDPIV ASIWKIQILS
410 420 430 440 450
TLINESNVKE IFKELKYYVA SAHFPENVVI MAVKSLSRCG QLSTSWESHV
460 470 480 490 500
MKWLIDHMES HNLSASVLDA YVNVIRMLVQ KNPTKHLRII FKLADLLTVQ
510 520 530 540 550
TSLADNARAG IVWLFGEIAS IEFKICPDVL RRLIQNFSNE GPETRCQILV
560 570 580 590 600
LSAKLLSYDI DNFKQAQVTG SEENNQNPPY YDFSGSRISQ MYNAVLYLAK
610 620 630 640 650
YDDEFDIRDR ARMISSLFDS GKYEIVSLLL QAPKPTARSD DFIVSARLET
660 670 680 690 700
HTPEIKEFFR MLPWNTEITE VGETGNDIRE GAELKDYNKY KKSFSSQSFI
710 720 730 740 750
TNNSARSFTS SSNAKLTGIN DGDSNSISGK GNVNTFTSQN GKKYRLQSLD
760 770 780 790 800
EFFSDIPERK SKPRKIIKVV EESSDEDEDE SEESSDDDEY SDSSLGTSSS

GTSSSHLEL
Length:809
Mass (Da):91,607
Last modified:October 1, 1996 - v2
Checksum:i475DA3A4F6E68E97
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 359TSKLGESSY → PLSWVNPP in AAC13877 (Ref. 1) Curated
Sequence conflicti724 – 7241S → T in AAC13877 (Ref. 1) Curated
Sequence conflicti798 – 8025Missing in AAC13877 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35411 Genomic DNA. Translation: AAC13877.1.
Y07777 Genomic DNA. Translation: CAA69083.1.
Z73046 Genomic DNA. Translation: CAA97290.1.
BK006941 Genomic DNA. Translation: DAA08351.1.
PIRiS64594.
RefSeqiNP_011777.3. NM_001181390.3.

Genome annotation databases

EnsemblFungiiYGR261C; YGR261C; YGR261C.
GeneIDi853177.
KEGGisce:YGR261C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35411 Genomic DNA. Translation: AAC13877.1.
Y07777 Genomic DNA. Translation: CAA69083.1.
Z73046 Genomic DNA. Translation: CAA97290.1.
BK006941 Genomic DNA. Translation: DAA08351.1.
PIRiS64594.
RefSeqiNP_011777.3. NM_001181390.3.

3D structure databases

ProteinModelPortaliP46682.
SMRiP46682. Positions 37-582.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33512. 97 interactions.
DIPiDIP-2684N.
IntActiP46682. 16 interactions.
MINTiMINT-530434.

PTM databases

iPTMnetiP46682.

Proteomic databases

MaxQBiP46682.
PeptideAtlasiP46682.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR261C; YGR261C; YGR261C.
GeneIDi853177.
KEGGisce:YGR261C.

Organism-specific databases

EuPathDBiFungiDB:YGR261C.
SGDiS000003493. APL6.

Phylogenomic databases

GeneTreeiENSGT00530000063546.
HOGENOMiHOG000248160.
InParanoidiP46682.
OMAiRKLVYIF.
OrthoDBiEOG74TX76.

Enzyme and pathway databases

BioCyciYEAST:G3O-30930-MONOMER.

Miscellaneous databases

NextBioi973308.
PROiP46682.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR026740. AP3_beta.
IPR026739. AP_beta.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
[Graphical view]
PANTHERiPTHR11134. PTHR11134. 4 hits.
PTHR11134:SF1. PTHR11134:SF1. 4 hits.
PfamiPF01602. Adaptin_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Suppressors of loss of yeast casein kinase 1 function define the four subunits of a novel putative adaptin complex."
    Robinson L.C., Engle H.M., Panek H.R.
    Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of an 11.6 kb region from the right arm of chromosome VII of Saccharomyces cerevisiae between the RAD2 and the MES1 genes reveals the presence of three new genes."
    Clemente M.L., Sartori G., Cardazzo B., Carignani G.
    Yeast 13:287-290(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The AP-3 adaptor complex is essential for cargo-selective transport to the yeast vacuole."
    Cowles C.R., Odorizzi G., Payne G.S., Emr S.D.
    Cell 91:109-118(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF THE AP-3 COMPLEX, FUNCTION OF THE AP-3 COMPLEX.
  6. "Suppressors of YCK-encoded yeast casein kinase 1 deficiency define the four subunits of a novel clathrin AP-like complex."
    Panek H.R., Stepp J.D., Engle H.M., Marks K.M., Tan P.K., Lemmon S.K., Robinson L.C.
    EMBO J. 16:4194-4204(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF THE AP-3 COMPLEX, FUNCTION OF THE AP-3 COMPLEX.
  7. "Formation of AP-3 transport intermediates requires Vps41 function."
    Rehling P., Darsow T., Katzmann D.J., Emr S.D.
    Nat. Cell Biol. 1:346-353(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION OF THE AP-3 COMPLEX.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-726, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-698, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-693; SER-698; SER-724 AND SER-726, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAP3B_YEAST
AccessioniPrimary (citable) accession number: P46682
Secondary accession number(s): D6VV40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3570 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.