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Reviewed, UniProtKB/Swiss-Prot P46681 (DLD2_YEAST)

Last modified January 19, 2010. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    D-lactate dehydrogenase [cytochrome] 2, mitochondrial
    EC=1.1.2.4
Alternative name(s):
    D-lactate ferricytochrome C oxidoreductase
      Short name=D-LCR
    Actin-interacting protein 2
Gene names
Name: DLD2
Synonyms: AIP2
Ordered Locus Names: YDL178W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length530 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

In addition to its enzymatic role it could play an important role in the yeast cell morphology. Ref.4

Catalytic activity

(R)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c.

Cofactor

FAD Potential.

Subunit structure

Interacts with F-actin. Ref.6

Subcellular location

Mitochondrion matrix Ref.4.

Miscellaneous

Present with 11400 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the FAD-binding oxidoreductase/transferase type 4 family.

Contains 1 FAD-binding PCMH-type domain.

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Ontologies

Keywords
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandFAD
Flavoprotein
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processlactate metabolic process Ref.4

Traceable author statement. Source: SGD

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentmitochondrial matrix Ref.4

Inferred from direct assay. Source: SGD

   Molecular functionD-lactate dehydrogenase (cytochrome) activity Ref.4

Inferred from direct assay. Source: SGD

FAD binding

Inferred from electronic annotation. Source: InterPro

actin binding Ref.6

Inferred from mutant phenotype. Source: SGD

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 530D-lactate dehydrogenase [cytochrome] 2, mitochondrialPRO_0000020429

Regions

Domain98 – 277180FAD-binding PCMH-type

Experimental info

Sequence conflict2391N → S in AAU09682. Ref.3

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Sequences

Sequence LengthMass (Da)Tools
P46681-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 42FDFBE5CA7E11B7

FASTA53059,268
        10         20         30         40         50         60 
MLRNILVRST GSNFKFAGRY MKSSALLGYY RRVNYYSTKI QTRLTSENYP DVHRDPRFKK 

        70         80         90        100        110        120 
LTSDDLNYFK SILSEQEILR ASESEDLSFY NEDWMRKYKG QSKLVLRPKS VEKVSLILNY 

       130        140        150        160        170        180 
CNDEKIAVVP QGGNTGLVGG SVPIFDELIL SLANLNKIRD FDPVSGILKC DAGVILENAN 

       190        200        210        220        230        240 
NYVMEQNYMF PLDLGAKGSC HVGGVVATNA GGLRLLRYGS LHGSVLGLEV VMPNGQIVNS 

       250        260        270        280        290        300 
MHSMRKDNTG YDLKQLFIGS EGTIGIITGV SILTVPKPKA FNVSYLSVES FEDVQKVFVR 

       310        320        330        340        350        360 
ARQELSEILS AFEFMDAKSQ VLAKSQLKDA AFPLEDEHPF YILIETSGSN KDHDDSKLET 

       370        380        390        400        410        420 
FLENVMEEGI VTDGVVAQDE TELQNLWKWR EMIPEASQAN GGVYKYDVSL PLKDLYSLVE 

       430        440        450        460        470        480 
ATNARLSEAE LVGDSPKPVV GAIGYGHVGD GNLHLNVAVR EYNKNIEKTL EPFVYEFVSS 

       490        500        510        520        530 
KHGSVSAEHG LGFQKKNYIG YSKSPEEVKM MKDLKVHYDP NGILNPYKYI

« Hide

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References

« Hide 'large scale' references
[1]"AIP1, AIP2 and AIP3 encode novel components of the yeast actin cytoskeleton."
Amberg D.C., Botstein D.
Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed: 9169867] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Signalling between mitochondria and the nucleus regulates the expression of a new D-lactate dehydrogenase activity in yeast."
Chelstowska A., Liu Z., Jia Y., Amberg D., Butow R.A.
Yeast 15:1377-1391(1999) [PubMed: 10509019] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Interaction of D-lactate dehydrogenase protein 2 (Dld2p) with F-actin: implication for an alternative function of Dld2p."
Hachiya N.S., Sakasegawa Y., Jozuka A., Tsukita S., Kaneko K.
Biochem. Biophys. Res. Commun. 319:78-82(2004) [PubMed: 15158445] [Abstract]
Cited for: INTERACTION WITH ACTIN.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U35667 Genomic DNA. Translation: AAA79142.1.
Z67750 Genomic DNA. Translation: CAA91567.1.
Z74226 Genomic DNA. Translation: CAA98752.1.
AY723765 Genomic DNA. Translation: AAU09682.1.
PIRS61034.
RefSeqNP_010103.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP-956N.
STRINGP46681.

Proteomic databases

PeptideAtlasP46681.

Genome annotation databases

EnsemblYDL178W; YDL178W; YDL178W; Saccharomyces cerevisiae. [Genome view]
GeneID851376.
KEGGsce:YDL178W.
NMPDRfig|4932.3.peg.837.

Organism-specific databases

CYGDYDL178w.
SGDS000002337. DLD2.

Phylogenomic databases

eggNOGfuNOG04802.
HOGENOMHBG553036.
OMALKYCNER.
OrthoDBEOG93N8VT.
PhylomeDBP46681.

Enzyme and pathway databases

BRENDA1.1.2.4. 250.

Gene expression databases

ArrayExpressP46681.
GenevestigatorP46681.
GermOnlineYDL178W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR016164. FAD-linked_Oxase-like_C.
IPR004113. FAD-linked_oxidase_C.
IPR006094. Oxid_FAD_bind_N.
[Graphical view]
Gene3DG3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit.
PfamPF02913. FAD-oxidase_C. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view]
PROSITEPS51387. FAD_PCMH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio968507.

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Entry information

Entry nameDLD2_YEAST
AccessionPrimary (citable) accession number: P46681
Secondary accession number(s): Q66RH8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 19, 2010
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents