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Protein

D-2-hydroxyglutarate--pyruvate transhydrogenase DLD2

Gene

DLD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of (R)-2-hydroxyglutarate to 2-oxoglutarate coupled to reduction of pyruvate to (R)-lactate. Can also use oxaloacetate as electron acceptor instead of pyruvate producing (R)-malate (PubMed:26774271). In addition to its enzymatic role it could play an important role in the yeast cell morphology (PubMed:10509019).2 Publications

Miscellaneous

Present with 11400 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

(R)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H+.
(R)-2-hydroxyglutarate + pyruvate = 2-oxoglutarate + (R)-lactate.1 Publication

Cofactori

FADCurated

Kineticsi

kcat is 0.11 sec(-1) for (R)-2-hydroxyglutarate oxidation with pyruvate as electron acceptor. kcat is 0.18 sec(-1) for (R)-2-hydroxyglutarate oxidation with DCIP as electron acceptor. kcat is 1.31 sec(-1) for (R)-lactate reduction with DCIP as electron donor.1 Publication
  1. KM=74 µM for (R)-2-hydroxyglutarate1 Publication
  2. KM=5.2 mM for (R)-lactate1 Publication

    GO - Molecular functioni

    • (R)-2-hydroxyglutarate dehydrogenase activity Source: SGD
    • actin binding Source: SGD
    • D-lactate dehydrogenase (cytochrome) activity Source: SGD
    • FAD binding Source: SGD

    GO - Biological processi

    • lactate catabolic process Source: SGD
    • lactate oxidation Source: GO_Central
    • respiratory electron transport chain Source: GO_Central

    Keywordsi

    Molecular functionOxidoreductase
    LigandFAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciYEAST:YDL178W-MONOMER.
    ReactomeiR-SCE-880009. Interconversion of 2-oxoglutarate and 2-hydroxyglutarate.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-2-hydroxyglutarate--pyruvate transhydrogenase DLD2Curated (EC:1.1.99.-1 Publication)
    Short name:
    D-2HG--pyruvate transhydrogenase DLD21 Publication
    Alternative name(s):
    Actin-interacting protein 2
    D-lactate dehydrogenase [cytochrome] 2, mitochondrial (EC:1.1.2.4)
    D-lactate ferricytochrome C oxidoreductase
    Short name:
    D-LCR
    Gene namesi
    Name:DLD2
    Synonyms:AIP2
    Ordered Locus Names:YDL178W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome IV

    Organism-specific databases

    EuPathDBiFungiDB:YDL178W.
    SGDiS000002337. DLD2.

    Subcellular locationi

    GO - Cellular componenti

    • mitochondrial matrix Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_0000020429? – 530D-2-hydroxyglutarate--pyruvate transhydrogenase DLD2
    Transit peptidei1 – ?MitochondrionSequence analysis

    Proteomic databases

    MaxQBiP46681.
    PRIDEiP46681.

    Interactioni

    Subunit structurei

    Interacts with F-actin.1 Publication

    GO - Molecular functioni

    • actin binding Source: SGD

    Protein-protein interaction databases

    BioGridi31888. 40 interactors.
    DIPiDIP-956N.
    MINTiMINT-4479766.

    Structurei

    3D structure databases

    ProteinModelPortaliP46681.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini98 – 277FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST180

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    GeneTreeiENSGT00550000075086.
    HOGENOMiHOG000230997.
    InParanoidiP46681.
    KOiK00102.
    OMAiNEDWMRK.
    OrthoDBiEOG092C1XYI.

    Family and domain databases

    Gene3Di1.10.45.10. 1 hit.
    3.30.43.10. 1 hit.
    3.30.465.10. 1 hit.
    InterProiView protein in InterPro
    IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR016164. FAD-linked_Oxase-like_C.
    IPR004113. FAD-linked_oxidase_C.
    IPR006094. Oxid_FAD_bind_N.
    IPR016171. Vanillyl_alc_oxidase_C-sub2.
    PfamiView protein in Pfam
    PF02913. FAD-oxidase_C. 1 hit.
    PF01565. FAD_binding_4. 1 hit.
    SUPFAMiSSF55103. SSF55103. 1 hit.
    SSF56176. SSF56176. 1 hit.
    PROSITEiView protein in PROSITE
    PS51387. FAD_PCMH. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P46681-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLRNILVRST GSNFKFAGRY MKSSALLGYY RRVNYYSTKI QTRLTSENYP
    60 70 80 90 100
    DVHRDPRFKK LTSDDLNYFK SILSEQEILR ASESEDLSFY NEDWMRKYKG
    110 120 130 140 150
    QSKLVLRPKS VEKVSLILNY CNDEKIAVVP QGGNTGLVGG SVPIFDELIL
    160 170 180 190 200
    SLANLNKIRD FDPVSGILKC DAGVILENAN NYVMEQNYMF PLDLGAKGSC
    210 220 230 240 250
    HVGGVVATNA GGLRLLRYGS LHGSVLGLEV VMPNGQIVNS MHSMRKDNTG
    260 270 280 290 300
    YDLKQLFIGS EGTIGIITGV SILTVPKPKA FNVSYLSVES FEDVQKVFVR
    310 320 330 340 350
    ARQELSEILS AFEFMDAKSQ VLAKSQLKDA AFPLEDEHPF YILIETSGSN
    360 370 380 390 400
    KDHDDSKLET FLENVMEEGI VTDGVVAQDE TELQNLWKWR EMIPEASQAN
    410 420 430 440 450
    GGVYKYDVSL PLKDLYSLVE ATNARLSEAE LVGDSPKPVV GAIGYGHVGD
    460 470 480 490 500
    GNLHLNVAVR EYNKNIEKTL EPFVYEFVSS KHGSVSAEHG LGFQKKNYIG
    510 520 530
    YSKSPEEVKM MKDLKVHYDP NGILNPYKYI
    Length:530
    Mass (Da):59,268
    Last modified:November 1, 1995 - v1
    Checksum:i42FDFBE5CA7E11B7
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti239N → S in AAU09682 (PubMed:17322287).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U35667 Genomic DNA. Translation: AAA79142.1.
    Z67750 Genomic DNA. Translation: CAA91567.1.
    Z74226 Genomic DNA. Translation: CAA98752.1.
    AY723765 Genomic DNA. Translation: AAU09682.1.
    BK006938 Genomic DNA. Translation: DAA11684.1.
    PIRiS61034.
    RefSeqiNP_010103.1. NM_001180238.1.

    Genome annotation databases

    EnsemblFungiiYDL178W; YDL178W; YDL178W.
    GeneIDi851376.
    KEGGisce:YDL178W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U35667 Genomic DNA. Translation: AAA79142.1.
    Z67750 Genomic DNA. Translation: CAA91567.1.
    Z74226 Genomic DNA. Translation: CAA98752.1.
    AY723765 Genomic DNA. Translation: AAU09682.1.
    BK006938 Genomic DNA. Translation: DAA11684.1.
    PIRiS61034.
    RefSeqiNP_010103.1. NM_001180238.1.

    3D structure databases

    ProteinModelPortaliP46681.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi31888. 40 interactors.
    DIPiDIP-956N.
    MINTiMINT-4479766.

    Proteomic databases

    MaxQBiP46681.
    PRIDEiP46681.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYDL178W; YDL178W; YDL178W.
    GeneIDi851376.
    KEGGisce:YDL178W.

    Organism-specific databases

    EuPathDBiFungiDB:YDL178W.
    SGDiS000002337. DLD2.

    Phylogenomic databases

    GeneTreeiENSGT00550000075086.
    HOGENOMiHOG000230997.
    InParanoidiP46681.
    KOiK00102.
    OMAiNEDWMRK.
    OrthoDBiEOG092C1XYI.

    Enzyme and pathway databases

    BioCyciYEAST:YDL178W-MONOMER.
    ReactomeiR-SCE-880009. Interconversion of 2-oxoglutarate and 2-hydroxyglutarate.

    Miscellaneous databases

    PROiPR:P46681.

    Family and domain databases

    Gene3Di1.10.45.10. 1 hit.
    3.30.43.10. 1 hit.
    3.30.465.10. 1 hit.
    InterProiView protein in InterPro
    IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR016164. FAD-linked_Oxase-like_C.
    IPR004113. FAD-linked_oxidase_C.
    IPR006094. Oxid_FAD_bind_N.
    IPR016171. Vanillyl_alc_oxidase_C-sub2.
    PfamiView protein in Pfam
    PF02913. FAD-oxidase_C. 1 hit.
    PF01565. FAD_binding_4. 1 hit.
    SUPFAMiSSF55103. SSF55103. 1 hit.
    SSF56176. SSF56176. 1 hit.
    PROSITEiView protein in PROSITE
    PS51387. FAD_PCMH. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiDLD2_YEAST
    AccessioniPrimary (citable) accession number: P46681
    Secondary accession number(s): D6VRH4, Q66RH8
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: March 15, 2017
    This is version 140 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.