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P46680 (AIP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Actin-interacting protein 1
Gene names
Name:AIP1
Ordered Locus Names:YMR092C
ORF Names:YM9582.17C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length615 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the depolymerization of actin filaments. Enhances the filament disassembly activity of cofilin and restricts cofilin localization to cortical actin patches. Ref.4

Subunit structure

Interacts with actin and cofilin in a ternary complex. Ref.4

Subcellular location

Cytoplasm › cytoskeleton. Cytoplasm › cytoskeleton › actin patch.

Miscellaneous

Present with 11100 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the WD repeat AIP1 family.

Contains 11 WD repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 615615Actin-interacting protein 1
PRO_0000050841

Regions

Repeat62 – 10948WD 1
Repeat114 – 15744WD 2
Repeat158 – 20144WD 3
Repeat204 – 24441WD 4
Repeat251 – 28939WD 5
Repeat337 – 36731WD 6
Repeat370 – 40637WD 7
Repeat442 – 48140WD 8
Repeat486 – 52540WD 9
Repeat529 – 57850WD 10
Repeat583 – 61331WD 11
Compositional bias363 – 3664Poly-Ser

Amino acid modifications

Modified residue3541Phosphoserine Ref.6
Modified residue3551Phosphotyrosine Ref.6

Secondary structure

1 ........................................................................................................................... 615
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P46680 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: B8340BF68DD08257

FASTA61567,326
        10         20         30         40         50         60 
MSSISLKEII PPQPSTQRNF TTHLSYDPTT NAIAYPCGKS AFVRCLDDGD SKVPPVVQFT 

        70         80         90        100        110        120 
GHGSSVVTTV KFSPIKGSQY LCSGDESGKV IVWGWTFDKE SNSVEVNVKS EFQVLAGPIS 

       130        140        150        160        170        180 
DISWDFEGRR LCVVGEGRDN FGVFISWDSG NSLGEVSGHS QRINACHLKQ SRPMRSMTVG 

       190        200        210        220        230        240 
DDGSVVFYQG PPFKFSASDR THHKQGSFVR DVEFSPDSGE FVITVGSDRK ISCFDGKSGE 

       250        260        270        280        290        300 
FLKYIEDDQE PVQGGIFALS WLDSQKFATV GADATIRVWD VTTSKCVQKW TLDKQQLGNQ 

       310        320        330        340        350        360 
QVGVVATGNG RIISLSLDGT LNFYELGHDE VLKTISGHNK GITALTVNPL ISGSYDGRIM 

       370        380        390        400        410        420 
EWSSSSMHQD HSNLIVSLDN SKAQEYSSIS WDDTLKVNGI TKHEFGSQPK VASANNDGFT 

       430        440        450        460        470        480 
AVLTNDDDLL ILQSFTGDII KSVRLNSPGS AVSLSQNYVA VGLEEGNTIQ VFKLSDLEVS 

       490        500        510        520        530        540 
FDLKTPLRAK PSYISISPSE TYIAAGDVMG KILLYDLQSR EVKTSRWAFH TSKINAISWK 

       550        560        570        580        590        600 
PAEKGANEEE IEEDLVATGS LDTNIFIYSV KRPMKIIKAL NAHKDGVNNL LWETPSTLVS 

       610 
SGADACIKRW NVVLE

« Hide

References

« Hide 'large scale' references
[1]Amberg D.C., Botstein D.
Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed: 9169872] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Aip1p interacts with cofilin to disassemble actin filaments."
Rodal A.A., Tetreault J.W., Lappalainen P., Drubin D.G., Amberg D.C.
J. Cell Biol. 145:1251-1264(1999) [PubMed: 10366597] [Abstract]
Cited for: INTERACTION WITH COFILIN, FUNCTION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354 AND TYR-355, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U35666 Genomic DNA. Translation: AAA79141.1.
Z49259 Genomic DNA. Translation: CAA89239.1.
BK006946 Genomic DNA. Translation: DAA09989.1.
PIRS54468.
RefSeqNP_013810.1. NM_001182592.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1PGUX-ray2.30A/B1-615[»]
1PI6X-ray2.50A1-615[»]
ProteinModelPortalP46680.
SMRP46680. Positions 2-615.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-890N.
IntActP46680. 7 interactions.
MINTMINT-399089.
STRINGP46680.

Proteomic databases

PeptideAtlasP46680.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR092C; YMR092C; YMR092C.
GeneID855117.
KEGGsce:YMR092C.
NMPDRfig|4932.3.peg.4857.

Organism-specific databases

CYGDYMR092c.
SGDS000004698. AIP1.

Phylogenomic databases

eggNOGfuNOG04700.
GeneTreeEFGT00050000000072.
HOGENOMHBG330599.
OMANSSCVAL.
OrthoDBEOG4P5PJ9.

Gene expression databases

ArrayExpressP46680.
GenevestigatorP46680.
GermOnlineYMR092C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR011046. WD40_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
Gene3DG3DSA:2.130.10.10. WD40/YVTN_repeat-like. 2 hits.
PfamPF00400. WD40. 6 hits.
[Graphical view]
SMARTSM00320. WD40. 10 hits.
[Graphical view]
SUPFAMSSF50978. WD40_like. 2 hits.
PROSITEPS00678. WD_REPEATS_1. False negative.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio978464.

Entry information

Entry nameAIP1_YEAST
AccessionPrimary (citable) accession number: P46680
Secondary accession number(s): D6VZR5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 25, 2012
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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