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P46677 (TAF1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription initiation factor TFIID subunit 1

EC=2.3.1.48
Alternative name(s):
TAFII-130
TAFII-145
TBP-associated factor 1
TBP-associated factor 145 kDa
Gene names
Name:TAF1
Synonyms:TAF130, TAF145
Ordered Locus Names:YGR274C
ORF Names:G9374
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1066 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. Ref.6 Ref.7 Ref.8 Ref.9

Catalytic activity

Acetyl-CoA + [histone] = CoA + acetyl-[histone].

Subunit structure

The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14. Ref.7

Subcellular location

Nucleus Ref.10.

Domain

Compared to higher eukaryotes TAF1, yeast TAF1 lacks the C-terminal bromodomains and C-terminal kinase activity. The TFIID interacting proteins BDF1 and BDF2 may substitute for these domains.

Miscellaneous

Present with 1500 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TAF1 family.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainCoiled coil
   Molecular functionAcyltransferase
Chromatin regulator
Transferase
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processRNA polymerase II transcriptional preinitiation complex assembly

Inferred from mutant phenotype PubMed 10713163PubMed 12600943PubMed 12840001. Source: SGD

histone acetylation

Inferred from direct assay Ref.6. Source: GOC

regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription from RNA polymerase II promoter

Inferred from direct assay Ref.8PubMed 15448131. Source: SGD

   Cellular_componentcytosol

Inferred from direct assay PubMed 22932476. Source: SGD

nucleus

Inferred from direct assay PubMed 22932476. Source: SGD

transcription factor TFIID complex

Inferred from direct assay Ref.7PubMed 15448131. Source: SGD

   Molecular_functionTBP-class protein binding

Inferred from direct assay PubMed 11677244. Source: SGD

chromatin binding

Inferred from direct assay PubMed 10817999PubMed 10818000PubMed 12600943. Source: SGD

histone acetyltransferase activity

Inferred from direct assay Ref.6. Source: SGD

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

protein binding

Inferred from physical interaction PubMed 10783167Ref.7PubMed 11805826PubMed 12582245PubMed 16429126PubMed 21179020PubMed 21734642PubMed 8083216. Source: IntAct

protein complex scaffold

Inferred from direct assay PubMed 15143185. Source: SGD

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10661066Transcription initiation factor TFIID subunit 1
PRO_0000118862

Regions

Coiled coil409 – 42517 Potential
Coiled coil944 – 100057 Potential
Compositional bias65 – 695Poly-Asp

Secondary structure

...... 1066
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P46677 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: FAF1EE2A9B2A7B73

FASTA1,066120,696
        10         20         30         40         50         60 
MVKQQGSGKT NLANEDEAYE AIFGGEFGSL EIGSYIGGDE GANSKDYTEH LPDAVDFEDE 

        70         80         90        100        110        120 
DELADDDDDL PEESDANLHP AMMTMGAYDD VNENGAVLGI DSNSLNMQLP EINGDLSQQF 

       130        140        150        160        170        180 
ILEDDGGTPA TSNALFMGMD ANEIHLATET GVLDGSGANE IGHSQLSIGG VNGNDMSING 

       190        200        210        220        230        240 
GFIMEPDMSD GKHKKATKLD LINHEKYLLK KYFPDFEKGK ILKWNKLIYR RSVPYHWHSE 

       250        260        270        280        290        300 
ISRVKKPFMP LNLKFKVQQD DKRLFNSRTI SYVAPIYQGK NNLLQSNSSA SRRGLIHVSI 

       310        320        330        340        350        360 
DELFPIKEQQ KKRKIIHDEK TISEDLLIAT DDWDQEKIIN QGTSSTATLA DSSMTPNLKF 

       370        380        390        400        410        420 
SGGYKLKSLI EDVAEDWQWD EDMIIDAKLK ESKHAELNMN DEKLLLMIEK TNNLAQQKQQ 

       430        440        450        460        470        480 
LDSSNLILPL NETILQQKFN LSNDDKYQIL KKTHQTKVRS TISNLNIQHS QPAINLQSPF 

       490        500        510        520        530        540 
YKVAVPRYQL RHFHRENFGS HIRPGTKIVF SKLKARKRKR DKGKDVKESF STSQDLTIGD 

       550        560        570        580        590        600 
TAPVYLMEYS EQTPVALSKF GMANKLINYY RKANEQDTLR PKLPVGETHV LGVQDKSPFW 

       610        620        630        640        650        660 
NFGFVEPGHI VPTLYNNMIR APVFKHDISG TDFLLTKSSG FGISNRFYLR NINHLFTVGQ 

       670        680        690        700        710        720 
TFPVEEIPGP NSRKVTSMKA TRLKMIIYRI LNHNHSKAIS IDPIAKHFPD QDYGQNRQKV 

       730        740        750        760        770        780 
KEFMKYQRDG PEKGLWRLKD DEKLLDNEAV KSLITPEQIS QVESMSQGLQ FQEDNEAYNF 

       790        800        810        820        830        840 
DSKLKSLEEN LLPWNITKNF INSTQMRAMI QIHGVGDPTG CGEGFSFLKT SMKGGFVKSG 

       850        860        870        880        890        900 
SPSSNNNSSN KKGTNTHSYN VAQQQKAYDE EIAKTWYTHT KSLSISNPFE EMTNPDEINQ 

       910        920        930        940        950        960 
TNKHVKTDRD DKKILKIVRK KRDENGIIQR QTIFIRDPRV IQGYIKIKEQ DKEDVNKLLE 

       970        980        990       1000       1010       1020 
EDTSKINNLE ELEKQKKLLQ LELANLEKSQ QRRAARQNSK RNGGATRTEN SVDNGSDLAG 

      1030       1040       1050       1060 
VTDGKAARNK GKNTTRRCAT CGQIGHIRTN KSCPMYSSKD NPASPK 

« Hide

References

« Hide 'large scale' references
[1]"Yeast TAFIIS in a multisubunit complex required for activated transcription."
Reese J.C., Apone L., Walker S.S., Griffin L.A., Green M.R.
Nature 371:523-527(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 583-599 AND 651-671.
Strain: Y57.
[2]"The sequence of a 8 kb segment on the right arm of yeast chromosome VII identifies four new open reading frames and the genes for yTAFII145."
Ruzzi M., Marconi A., Saliola M., Fabiani L., Montebove F., Frontali L.
Yeast 13:365-368(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Identification and characterization of a TFIID-like multiprotein complex from Saccharomyces cerevisiae."
Poon D., Bai Y., Campbell A.M., Bjorklund S., Kim Y.-J., Zhou S., Kornberg R.D., Weil P.A.
Proc. Natl. Acad. Sci. U.S.A. 92:8224-8228(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 368-384; 528-554 AND 752-783, CHARACTERIZATION.
Strain: ATCC 76621 / YPH252.
[6]"The TAF(II)250 subunit of TFIID has histone acetyltransferase activity."
Mizzen C.A., Yang X.J., Kokubo T., Brownell J.E., Bannister A.J., Owen-Hughes T., Workman J., Wang L., Berger S.L., Kouzarides T., Nakatani Y., Allis C.D.
Cell 87:1261-1270(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TAF1 ACETYLATION ACTIVITY.
[7]"Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex."
Sanders S.L., Weil P.A.
J. Biol. Chem. 275:13895-13900(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[8]"Molecular characterization of Saccharomyces cerevisiae TFIID."
Sanders S.L., Garbett K.A., Weil P.A.
Mol. Cell. Biol. 22:6000-6013(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TFIID STOICHIOMETRY.
[9]"Multi-protein complexes in eukaryotic gene transcription."
Martinez E.
Plant Mol. Biol. 50:925-947(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]"Mapping histone fold TAFs within yeast TFIID."
Leurent C., Sanders S.L., Ruhlmann C., Mallouh V., Weil P.A., Kirschner D.B., Tora L., Schultz P.
EMBO J. 21:3424-3433(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U14954 Genomic DNA. Translation: AAA79178.1.
X84098 Genomic DNA. Translation: CAA58896.1.
Z73059 Genomic DNA. Translation: CAA97304.1.
BK006941 Genomic DNA. Translation: DAA08362.1.
PIRS50237.
RefSeqNP_011790.1. NM_001181403.2.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4B0AX-ray1.97A8-71[»]
ProteinModelPortalP46677.
SMRP46677. Positions 9-66.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33524. 757 interactions.
DIPDIP-839N.
IntActP46677. 76 interactions.
MINTMINT-617790.
STRING4932.YGR274C.

Proteomic databases

MaxQBP46677.
PaxDbP46677.
PeptideAtlasP46677.
PRIDEP46677.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR274C; YGR274C; YGR274C.
GeneID853191.
KEGGsce:YGR274C.

Organism-specific databases

CYGDYGR274c.
SGDS000003506. TAF1.

Phylogenomic databases

eggNOGCOG5179.
GeneTreeENSGT00390000012659.
HOGENOMHOG000216192.
KOK03125.
OMAAPHSRKV.
OrthoDBEOG7PCJSB.

Enzyme and pathway databases

BioCycYEAST:G3O-30939-MONOMER.

Gene expression databases

GenevestigatorP46677.

Family and domain databases

InterProIPR022591. TFIID_sub1_DUF3591.
IPR001878. Znf_CCHC.
[Graphical view]
PfamPF12157. DUF3591. 1 hit.
[Graphical view]
SMARTSM00343. ZnF_C2HC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973344.
PROP46677.

Entry information

Entry nameTAF1_YEAST
AccessionPrimary (citable) accession number: P46677
Secondary accession number(s): D6VV51
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references