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P46677

- TAF1_YEAST

UniProt

P46677 - TAF1_YEAST

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Protein

Transcription initiation factor TFIID subunit 1

Gene
TAF1, TAF130, TAF145, YGR274C, G9374
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.4 Publications

Catalytic activityi

Acetyl-CoA + [histone] = CoA + acetyl-[histone].

GO - Molecular functioni

  1. chromatin binding Source: SGD
  2. histone acetyltransferase activity Source: SGD
  3. nucleic acid binding Source: InterPro
  4. protein binding Source: IntAct
  5. protein complex scaffold Source: SGD
  6. TBP-class protein binding Source: SGD
  7. zinc ion binding Source: InterPro

GO - Biological processi

  1. histone acetylation Source: GOC
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  4. transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Chromatin regulator, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30939-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 1 (EC:2.3.1.48)
Alternative name(s):
TAFII-130
TAFII-145
TBP-associated factor 1
TBP-associated factor 145 kDa
Gene namesi
Name:TAF1
Synonyms:TAF130, TAF145
Ordered Locus Names:YGR274C
ORF Names:G9374
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR274c.
SGDiS000003506. TAF1.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. cytosol Source: SGD
  2. nucleus Source: SGD
  3. transcription factor TFIID complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10661066Transcription initiation factor TFIID subunit 1PRO_0000118862Add
BLAST

Proteomic databases

MaxQBiP46677.
PaxDbiP46677.
PeptideAtlasiP46677.
PRIDEiP46677.

Expressioni

Gene expression databases

GenevestigatoriP46677.

Interactioni

Subunit structurei

The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BDF1P358173EBI-18855,EBI-3493
TAF2P232554EBI-18855,EBI-18862
TAF4P501057EBI-18855,EBI-11231
TAF6P530408EBI-18855,EBI-18876

Protein-protein interaction databases

BioGridi33524. 757 interactions.
DIPiDIP-839N.
IntActiP46677. 76 interactions.
MINTiMINT-617790.
STRINGi4932.YGR274C.

Structurei

Secondary structure

1
1066
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi15 – 239
Beta strandi25 – 273
Helixi28 – 358

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4B0AX-ray1.97A8-71[»]
ProteinModelPortaliP46677.
SMRiP46677. Positions 9-66.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili409 – 42517 Reviewed predictionAdd
BLAST
Coiled coili944 – 100057 Reviewed predictionAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi65 – 695Poly-Asp

Domaini

Compared to higher eukaryotes TAF1, yeast TAF1 lacks the C-terminal bromodomains and C-terminal kinase activity. The TFIID interacting proteins BDF1 and BDF2 may substitute for these domains.

Sequence similaritiesi

Belongs to the TAF1 family.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5179.
GeneTreeiENSGT00390000012659.
HOGENOMiHOG000216192.
KOiK03125.
OMAiAPHSRKV.
OrthoDBiEOG7PCJSB.

Family and domain databases

InterProiIPR022591. TFIID_sub1_DUF3591.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF12157. DUF3591. 1 hit.
[Graphical view]
SMARTiSM00343. ZnF_C2HC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46677-1 [UniParc]FASTAAdd to Basket

« Hide

MVKQQGSGKT NLANEDEAYE AIFGGEFGSL EIGSYIGGDE GANSKDYTEH     50
LPDAVDFEDE DELADDDDDL PEESDANLHP AMMTMGAYDD VNENGAVLGI 100
DSNSLNMQLP EINGDLSQQF ILEDDGGTPA TSNALFMGMD ANEIHLATET 150
GVLDGSGANE IGHSQLSIGG VNGNDMSING GFIMEPDMSD GKHKKATKLD 200
LINHEKYLLK KYFPDFEKGK ILKWNKLIYR RSVPYHWHSE ISRVKKPFMP 250
LNLKFKVQQD DKRLFNSRTI SYVAPIYQGK NNLLQSNSSA SRRGLIHVSI 300
DELFPIKEQQ KKRKIIHDEK TISEDLLIAT DDWDQEKIIN QGTSSTATLA 350
DSSMTPNLKF SGGYKLKSLI EDVAEDWQWD EDMIIDAKLK ESKHAELNMN 400
DEKLLLMIEK TNNLAQQKQQ LDSSNLILPL NETILQQKFN LSNDDKYQIL 450
KKTHQTKVRS TISNLNIQHS QPAINLQSPF YKVAVPRYQL RHFHRENFGS 500
HIRPGTKIVF SKLKARKRKR DKGKDVKESF STSQDLTIGD TAPVYLMEYS 550
EQTPVALSKF GMANKLINYY RKANEQDTLR PKLPVGETHV LGVQDKSPFW 600
NFGFVEPGHI VPTLYNNMIR APVFKHDISG TDFLLTKSSG FGISNRFYLR 650
NINHLFTVGQ TFPVEEIPGP NSRKVTSMKA TRLKMIIYRI LNHNHSKAIS 700
IDPIAKHFPD QDYGQNRQKV KEFMKYQRDG PEKGLWRLKD DEKLLDNEAV 750
KSLITPEQIS QVESMSQGLQ FQEDNEAYNF DSKLKSLEEN LLPWNITKNF 800
INSTQMRAMI QIHGVGDPTG CGEGFSFLKT SMKGGFVKSG SPSSNNNSSN 850
KKGTNTHSYN VAQQQKAYDE EIAKTWYTHT KSLSISNPFE EMTNPDEINQ 900
TNKHVKTDRD DKKILKIVRK KRDENGIIQR QTIFIRDPRV IQGYIKIKEQ 950
DKEDVNKLLE EDTSKINNLE ELEKQKKLLQ LELANLEKSQ QRRAARQNSK 1000
RNGGATRTEN SVDNGSDLAG VTDGKAARNK GKNTTRRCAT CGQIGHIRTN 1050
KSCPMYSSKD NPASPK 1066
Length:1,066
Mass (Da):120,696
Last modified:November 1, 1995 - v1
Checksum:iFAF1EE2A9B2A7B73
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U14954 Genomic DNA. Translation: AAA79178.1.
X84098 Genomic DNA. Translation: CAA58896.1.
Z73059 Genomic DNA. Translation: CAA97304.1.
BK006941 Genomic DNA. Translation: DAA08362.1.
PIRiS50237.
RefSeqiNP_011790.1. NM_001181403.2.

Genome annotation databases

EnsemblFungiiYGR274C; YGR274C; YGR274C.
GeneIDi853191.
KEGGisce:YGR274C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U14954 Genomic DNA. Translation: AAA79178.1 .
X84098 Genomic DNA. Translation: CAA58896.1 .
Z73059 Genomic DNA. Translation: CAA97304.1 .
BK006941 Genomic DNA. Translation: DAA08362.1 .
PIRi S50237.
RefSeqi NP_011790.1. NM_001181403.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4B0A X-ray 1.97 A 8-71 [» ]
ProteinModelPortali P46677.
SMRi P46677. Positions 9-66.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33524. 757 interactions.
DIPi DIP-839N.
IntActi P46677. 76 interactions.
MINTi MINT-617790.
STRINGi 4932.YGR274C.

Proteomic databases

MaxQBi P46677.
PaxDbi P46677.
PeptideAtlasi P46677.
PRIDEi P46677.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR274C ; YGR274C ; YGR274C .
GeneIDi 853191.
KEGGi sce:YGR274C.

Organism-specific databases

CYGDi YGR274c.
SGDi S000003506. TAF1.

Phylogenomic databases

eggNOGi COG5179.
GeneTreei ENSGT00390000012659.
HOGENOMi HOG000216192.
KOi K03125.
OMAi APHSRKV.
OrthoDBi EOG7PCJSB.

Enzyme and pathway databases

BioCyci YEAST:G3O-30939-MONOMER.

Miscellaneous databases

NextBioi 973344.
PROi P46677.

Gene expression databases

Genevestigatori P46677.

Family and domain databases

InterProi IPR022591. TFIID_sub1_DUF3591.
IPR001878. Znf_CCHC.
[Graphical view ]
Pfami PF12157. DUF3591. 1 hit.
[Graphical view ]
SMARTi SM00343. ZnF_C2HC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast TAFIIS in a multisubunit complex required for activated transcription."
    Reese J.C., Apone L., Walker S.S., Griffin L.A., Green M.R.
    Nature 371:523-527(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 583-599 AND 651-671.
    Strain: Y57.
  2. "The sequence of a 8 kb segment on the right arm of yeast chromosome VII identifies four new open reading frames and the genes for yTAFII145."
    Ruzzi M., Marconi A., Saliola M., Fabiani L., Montebove F., Frontali L.
    Yeast 13:365-368(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Identification and characterization of a TFIID-like multiprotein complex from Saccharomyces cerevisiae."
    Poon D., Bai Y., Campbell A.M., Bjorklund S., Kim Y.-J., Zhou S., Kornberg R.D., Weil P.A.
    Proc. Natl. Acad. Sci. U.S.A. 92:8224-8228(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 368-384; 528-554 AND 752-783, CHARACTERIZATION.
    Strain: ATCC 76621 / YPH252.
  6. Cited for: FUNCTION, TAF1 ACETYLATION ACTIVITY.
  7. "Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex."
    Sanders S.L., Weil P.A.
    J. Biol. Chem. 275:13895-13900(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  8. "Molecular characterization of Saccharomyces cerevisiae TFIID."
    Sanders S.L., Garbett K.A., Weil P.A.
    Mol. Cell. Biol. 22:6000-6013(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TFIID STOICHIOMETRY.
  9. "Multi-protein complexes in eukaryotic gene transcription."
    Martinez E.
    Plant Mol. Biol. 50:925-947(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  12. Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.

Entry informationi

Entry nameiTAF1_YEAST
AccessioniPrimary (citable) accession number: P46677
Secondary accession number(s): D6VV51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1500 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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