P46677 (TAF1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription initiation factor TFIID subunit 1
EC=2.3.1.48

Alternative name(s):
TAFII-130
TAFII-145
TBP-associated factor 1
TBP-associated factor 145 kDa

Gene names

Name:	TAF1
Synonyms:	TAF130, TAF145
Ordered Locus Names:	YGR274C
ORF Names:	G9374

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1066 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. Ref.6 Ref.7 Ref.8 Ref.9
Catalytic activity	Acetyl-CoA + [histone] = CoA + acetyl-[histone].
Subunit structure	The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14. Ref.7
Subcellular location	Nucleus Ref.10.
Domain	Compared to higher eukaryotes TAF1, yeast TAF1 lacks the C-terminal bromodomains and C-terminal kinase activity. The TFIID interacting proteins BDF1 and BDF2 may substitute for these domains.
Miscellaneous	Present with 1500 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the TAF1 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil
Molecular function	Acyltransferase Chromatin regulator Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	RNA polymerase II transcriptional preinitiation complex assembly Inferred from mutant phenotype PubMed 10713163PubMed 12600943PubMed 12840001. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	transcription factor TFIID complex Inferred from direct assay Ref.7PubMed 15448131. Source: SGD
Molecular_function	TBP-class protein binding Inferred from direct assay PubMed 11677244. Source: SGD chromatin binding Inferred from direct assay PubMed 10817999PubMed 10818000PubMed 12600943. Source: SGD histone acetyltransferase activity Inferred from direct assay Ref.6. Source: SGD nucleic acid binding Inferred from electronic annotation. Source: InterPro protein complex scaffold Inferred from direct assay PubMed 15143185. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BDF1	P35817	3	EBI-18855,EBI-3493
TAF2	P23255	4	EBI-18855,EBI-18862
TAF4	P50105	7	EBI-18855,EBI-11231
TAF6	P53040	8	EBI-18855,EBI-18876

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1066	1066	Transcription initiation factor TFIID subunit 1		PRO_0000118862
Regions
	Coiled coil	409 – 425	17	Potential
	Coiled coil	944 – 1000	57	Potential
	Compositional bias	65 – 69	5	Poly-Asp
Amino acid modifications
	Modified residue	478	1	Phosphoserine Ref.13

Sequences

	Sequence		Length	Mass (Da)
	P46677 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: FAF1EE2A9B2A7B73	FASTA	1,066	120,696
	10 20 30 40 50 60 MVKQQGSGKT NLANEDEAYE AIFGGEFGSL EIGSYIGGDE GANSKDYTEH LPDAVDFEDE 70 80 90 100 110 120 DELADDDDDL PEESDANLHP AMMTMGAYDD VNENGAVLGI DSNSLNMQLP EINGDLSQQF 130 140 150 160 170 180 ILEDDGGTPA TSNALFMGMD ANEIHLATET GVLDGSGANE IGHSQLSIGG VNGNDMSING 190 200 210 220 230 240 GFIMEPDMSD GKHKKATKLD LINHEKYLLK KYFPDFEKGK ILKWNKLIYR RSVPYHWHSE 250 260 270 280 290 300 ISRVKKPFMP LNLKFKVQQD DKRLFNSRTI SYVAPIYQGK NNLLQSNSSA SRRGLIHVSI 310 320 330 340 350 360 DELFPIKEQQ KKRKIIHDEK TISEDLLIAT DDWDQEKIIN QGTSSTATLA DSSMTPNLKF 370 380 390 400 410 420 SGGYKLKSLI EDVAEDWQWD EDMIIDAKLK ESKHAELNMN DEKLLLMIEK TNNLAQQKQQ 430 440 450 460 470 480 LDSSNLILPL NETILQQKFN LSNDDKYQIL KKTHQTKVRS TISNLNIQHS QPAINLQSPF 490 500 510 520 530 540 YKVAVPRYQL RHFHRENFGS HIRPGTKIVF SKLKARKRKR DKGKDVKESF STSQDLTIGD 550 560 570 580 590 600 TAPVYLMEYS EQTPVALSKF GMANKLINYY RKANEQDTLR PKLPVGETHV LGVQDKSPFW 610 620 630 640 650 660 NFGFVEPGHI VPTLYNNMIR APVFKHDISG TDFLLTKSSG FGISNRFYLR NINHLFTVGQ 670 680 690 700 710 720 TFPVEEIPGP NSRKVTSMKA TRLKMIIYRI LNHNHSKAIS IDPIAKHFPD QDYGQNRQKV 730 740 750 760 770 780 KEFMKYQRDG PEKGLWRLKD DEKLLDNEAV KSLITPEQIS QVESMSQGLQ FQEDNEAYNF 790 800 810 820 830 840 DSKLKSLEEN LLPWNITKNF INSTQMRAMI QIHGVGDPTG CGEGFSFLKT SMKGGFVKSG 850 860 870 880 890 900 SPSSNNNSSN KKGTNTHSYN VAQQQKAYDE EIAKTWYTHT KSLSISNPFE EMTNPDEINQ 910 920 930 940 950 960 TNKHVKTDRD DKKILKIVRK KRDENGIIQR QTIFIRDPRV IQGYIKIKEQ DKEDVNKLLE 970 980 990 1000 1010 1020 EDTSKINNLE ELEKQKKLLQ LELANLEKSQ QRRAARQNSK RNGGATRTEN SVDNGSDLAG 1030 1040 1050 1060 VTDGKAARNK GKNTTRRCAT CGQIGHIRTN KSCPMYSSKD NPASPK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast TAFIIS in a multisubunit complex required for activated transcription." Reese J.C., Apone L., Walker S.S., Griffin L.A., Green M.R. Nature 371:523-527(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 583-599 AND 651-671. Strain: Y57.
[2]	"The sequence of a 8 kb segment on the right arm of yeast chromosome VII identifies four new open reading frames and the genes for yTAFII145." Ruzzi M., Marconi A., Saliola M., Fabiani L., Montebove F., Frontali L. Yeast 13:365-368(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Identification and characterization of a TFIID-like multiprotein complex from Saccharomyces cerevisiae." Poon D., Bai Y., Campbell A.M., Bjorklund S., Kim Y.-J., Zhou S., Kornberg R.D., Weil P.A. Proc. Natl. Acad. Sci. U.S.A. 92:8224-8228(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 368-384; 528-554 AND 752-783, CHARACTERIZATION. Strain: ATCC 76621 / YPH252.
[6]	"The TAF(II)250 subunit of TFIID has histone acetyltransferase activity." Mizzen C.A., Yang X.J., Kokubo T., Brownell J.E., Bannister A.J., Owen-Hughes T., Workman J., Wang L., Berger S.L., Kouzarides T., Nakatani Y., Allis C.D. Cell 87:1261-1270(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TAF1 ACETYLATION ACTIVITY.
[7]	"Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex." Sanders S.L., Weil P.A. J. Biol. Chem. 275:13895-13900(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT.
[8]	"Molecular characterization of Saccharomyces cerevisiae TFIID." Sanders S.L., Garbett K.A., Weil P.A. Mol. Cell. Biol. 22:6000-6013(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TFIID STOICHIOMETRY.
[9]	"Multi-protein complexes in eukaryotic gene transcription." Martinez E. Plant Mol. Biol. 50:925-947(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]	"Mapping histone fold TAFs within yeast TFIID." Leurent C., Sanders S.L., Ruhlmann C., Mallouh V., Weil P.A., Kirschner D.B., Tora L., Schultz P. EMBO J. 21:3424-3433(2002) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-478, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14954 Genomic DNA. Translation: AAA79178.1. X84098 Genomic DNA. Translation: CAA58896.1. Z73059 Genomic DNA. Translation: CAA97304.1. BK006941 Genomic DNA. Translation: DAA08362.1.
PIR	S50237.
RefSeq	NP_011790.1. NM_001181403.2.
3D structure databases
ProteinModelPortal	P46677.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-839N.
IntAct	P46677. 75 interactions.
MINT	MINT-617790.
STRING	4932.YGR274C.
Proteomic databases
PaxDb	P46677.
PeptideAtlas	P46677.
PRIDE	P46677.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR274C; YGR274C; YGR274C.
GeneID	853191.
KEGG	sce:YGR274C.
Organism-specific databases
CYGD	YGR274c.
SGD	S000003506. TAF1.
Phylogenomic databases
eggNOG	COG5179.
GeneTree	ENSGT00390000012659.
HOGENOM	HOG000216192.
KO	K03125.
OMA	NTTRRCA.
OrthoDB	EOG4ZW8KG.
Gene expression databases
Genevestigator	P46677.
GermOnline	YGR274C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022591. TFIID_sub1_DUF3591. IPR001878. Znf_CCHC. [Graphical view]
Pfam	PF12157. DUF3591. 1 hit. [Graphical view]
SMART	SM00343. ZnF_C2HC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973344.

Entry information

Entry name

TAF1_YEAST

Accession

Primary (citable) accession number: P46677
Secondary accession number(s): D6VV51

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program