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P46677

- TAF1_YEAST

UniProt

P46677 - TAF1_YEAST

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Protein

Transcription initiation factor TFIID subunit 1

Gene

TAF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.4 Publications

Catalytic activityi

Acetyl-CoA + [histone] = CoA + acetyl-[histone].

GO - Molecular functioni

  1. chromatin binding Source: SGD
  2. histone acetyltransferase activity Source: SGD
  3. nucleic acid binding Source: InterPro
  4. protein complex scaffold Source: SGD
  5. TBP-class protein binding Source: SGD
  6. zinc ion binding Source: InterPro

GO - Biological processi

  1. histone acetylation Source: GOC
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  4. transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Chromatin regulator, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30939-MONOMER.
ReactomeiREACT_229646. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_236803. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_249293. RNA Polymerase II Transcription Initiation.
REACT_250507. RNA Polymerase II Pre-transcription Events.
REACT_257112. RNA Polymerase II Promoter Escape.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 1 (EC:2.3.1.48)
Alternative name(s):
TAFII-130
TAFII-145
TBP-associated factor 1
TBP-associated factor 145 kDa
Gene namesi
Name:TAF1
Synonyms:TAF130, TAF145
Ordered Locus Names:YGR274C
ORF Names:G9374
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR274c.
SGDiS000003506. TAF1.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. cytosol Source: SGD
  2. nucleus Source: SGD
  3. transcription factor TFIID complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10661066Transcription initiation factor TFIID subunit 1PRO_0000118862Add
BLAST

Proteomic databases

MaxQBiP46677.
PaxDbiP46677.
PeptideAtlasiP46677.
PRIDEiP46677.

Expressioni

Gene expression databases

GenevestigatoriP46677.

Interactioni

Subunit structurei

The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BDF1P358173EBI-18855,EBI-3493
TAF2P232554EBI-18855,EBI-18862
TAF4P501057EBI-18855,EBI-11231
TAF6P530408EBI-18855,EBI-18876

Protein-protein interaction databases

BioGridi33524. 757 interactions.
DIPiDIP-839N.
IntActiP46677. 76 interactions.
MINTiMINT-617790.
STRINGi4932.YGR274C.

Structurei

Secondary structure

1
1066
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi15 – 239Combined sources
Beta strandi25 – 273Combined sources
Helixi28 – 358Combined sources
Helixi471 – 4755Combined sources
Turni478 – 4803Combined sources
Beta strandi481 – 4844Combined sources
Helixi488 – 4914Combined sources
Turni492 – 4943Combined sources
Turni498 – 5014Combined sources
Beta strandi507 – 5104Combined sources
Helixi518 – 5214Combined sources
Helixi526 – 5294Combined sources
Helixi533 – 5364Combined sources
Beta strandi538 – 5425Combined sources
Beta strandi544 – 55310Combined sources
Beta strandi563 – 5719Combined sources
Beta strandi584 – 5918Combined sources
Turni598 – 6014Combined sources
Beta strandi610 – 6156Combined sources
Beta strandi617 – 62610Combined sources
Beta strandi632 – 6409Combined sources
Beta strandi643 – 6508Combined sources
Beta strandi653 – 6586Combined sources
Beta strandi662 – 6654Combined sources
Helixi673 – 69422Combined sources
Helixi702 – 7054Combined sources
Helixi713 – 7208Combined sources
Turni721 – 7233Combined sources
Beta strandi724 – 7263Combined sources
Turni731 – 7344Combined sources
Beta strandi735 – 7384Combined sources
Helixi747 – 7504Combined sources
Helixi751 – 7533Combined sources
Helixi756 – 77823Combined sources
Helixi782 – 7909Combined sources
Helixi793 – 80412Combined sources
Beta strandi820 – 8245Combined sources
Helixi859 – 88224Combined sources
Helixi888 – 8914Combined sources
Helixi900 – 9034Combined sources
Beta strandi914 – 9229Combined sources
Beta strandi926 – 93510Combined sources
Helixi938 – 95114Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4B0AX-ray1.97A8-71[»]
4OY2X-ray2.90A/C/E455-960[»]
ProteinModelPortaliP46677.
SMRiP46677. Positions 9-66, 464-956.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili409 – 42517Sequence AnalysisAdd
BLAST
Coiled coili944 – 100057Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi65 – 695Poly-Asp

Domaini

Compared to higher eukaryotes TAF1, yeast TAF1 lacks the C-terminal bromodomains and C-terminal kinase activity. The TFIID interacting proteins BDF1 and BDF2 may substitute for these domains.

Sequence similaritiesi

Belongs to the TAF1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5179.
GeneTreeiENSGT00390000012659.
HOGENOMiHOG000216192.
InParanoidiP46677.
KOiK03125.
OMAiAPHSRKV.
OrthoDBiEOG7PCJSB.

Family and domain databases

InterProiIPR022591. TFIID_sub1_DUF3591.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF12157. DUF3591. 1 hit.
[Graphical view]
SMARTiSM00343. ZnF_C2HC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46677-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVKQQGSGKT NLANEDEAYE AIFGGEFGSL EIGSYIGGDE GANSKDYTEH
60 70 80 90 100
LPDAVDFEDE DELADDDDDL PEESDANLHP AMMTMGAYDD VNENGAVLGI
110 120 130 140 150
DSNSLNMQLP EINGDLSQQF ILEDDGGTPA TSNALFMGMD ANEIHLATET
160 170 180 190 200
GVLDGSGANE IGHSQLSIGG VNGNDMSING GFIMEPDMSD GKHKKATKLD
210 220 230 240 250
LINHEKYLLK KYFPDFEKGK ILKWNKLIYR RSVPYHWHSE ISRVKKPFMP
260 270 280 290 300
LNLKFKVQQD DKRLFNSRTI SYVAPIYQGK NNLLQSNSSA SRRGLIHVSI
310 320 330 340 350
DELFPIKEQQ KKRKIIHDEK TISEDLLIAT DDWDQEKIIN QGTSSTATLA
360 370 380 390 400
DSSMTPNLKF SGGYKLKSLI EDVAEDWQWD EDMIIDAKLK ESKHAELNMN
410 420 430 440 450
DEKLLLMIEK TNNLAQQKQQ LDSSNLILPL NETILQQKFN LSNDDKYQIL
460 470 480 490 500
KKTHQTKVRS TISNLNIQHS QPAINLQSPF YKVAVPRYQL RHFHRENFGS
510 520 530 540 550
HIRPGTKIVF SKLKARKRKR DKGKDVKESF STSQDLTIGD TAPVYLMEYS
560 570 580 590 600
EQTPVALSKF GMANKLINYY RKANEQDTLR PKLPVGETHV LGVQDKSPFW
610 620 630 640 650
NFGFVEPGHI VPTLYNNMIR APVFKHDISG TDFLLTKSSG FGISNRFYLR
660 670 680 690 700
NINHLFTVGQ TFPVEEIPGP NSRKVTSMKA TRLKMIIYRI LNHNHSKAIS
710 720 730 740 750
IDPIAKHFPD QDYGQNRQKV KEFMKYQRDG PEKGLWRLKD DEKLLDNEAV
760 770 780 790 800
KSLITPEQIS QVESMSQGLQ FQEDNEAYNF DSKLKSLEEN LLPWNITKNF
810 820 830 840 850
INSTQMRAMI QIHGVGDPTG CGEGFSFLKT SMKGGFVKSG SPSSNNNSSN
860 870 880 890 900
KKGTNTHSYN VAQQQKAYDE EIAKTWYTHT KSLSISNPFE EMTNPDEINQ
910 920 930 940 950
TNKHVKTDRD DKKILKIVRK KRDENGIIQR QTIFIRDPRV IQGYIKIKEQ
960 970 980 990 1000
DKEDVNKLLE EDTSKINNLE ELEKQKKLLQ LELANLEKSQ QRRAARQNSK
1010 1020 1030 1040 1050
RNGGATRTEN SVDNGSDLAG VTDGKAARNK GKNTTRRCAT CGQIGHIRTN
1060
KSCPMYSSKD NPASPK
Length:1,066
Mass (Da):120,696
Last modified:November 1, 1995 - v1
Checksum:iFAF1EE2A9B2A7B73
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14954 Genomic DNA. Translation: AAA79178.1.
X84098 Genomic DNA. Translation: CAA58896.1.
Z73059 Genomic DNA. Translation: CAA97304.1.
BK006941 Genomic DNA. Translation: DAA08362.1.
PIRiS50237.
RefSeqiNP_011790.1. NM_001181403.2.

Genome annotation databases

EnsemblFungiiYGR274C; YGR274C; YGR274C.
GeneIDi853191.
KEGGisce:YGR274C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14954 Genomic DNA. Translation: AAA79178.1 .
X84098 Genomic DNA. Translation: CAA58896.1 .
Z73059 Genomic DNA. Translation: CAA97304.1 .
BK006941 Genomic DNA. Translation: DAA08362.1 .
PIRi S50237.
RefSeqi NP_011790.1. NM_001181403.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4B0A X-ray 1.97 A 8-71 [» ]
4OY2 X-ray 2.90 A/C/E 455-960 [» ]
ProteinModelPortali P46677.
SMRi P46677. Positions 9-66, 464-956.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33524. 757 interactions.
DIPi DIP-839N.
IntActi P46677. 76 interactions.
MINTi MINT-617790.
STRINGi 4932.YGR274C.

Proteomic databases

MaxQBi P46677.
PaxDbi P46677.
PeptideAtlasi P46677.
PRIDEi P46677.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR274C ; YGR274C ; YGR274C .
GeneIDi 853191.
KEGGi sce:YGR274C.

Organism-specific databases

CYGDi YGR274c.
SGDi S000003506. TAF1.

Phylogenomic databases

eggNOGi COG5179.
GeneTreei ENSGT00390000012659.
HOGENOMi HOG000216192.
InParanoidi P46677.
KOi K03125.
OMAi APHSRKV.
OrthoDBi EOG7PCJSB.

Enzyme and pathway databases

BioCyci YEAST:G3O-30939-MONOMER.
Reactomei REACT_229646. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_236803. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_249293. RNA Polymerase II Transcription Initiation.
REACT_250507. RNA Polymerase II Pre-transcription Events.
REACT_257112. RNA Polymerase II Promoter Escape.

Miscellaneous databases

NextBioi 973344.
PROi P46677.

Gene expression databases

Genevestigatori P46677.

Family and domain databases

InterProi IPR022591. TFIID_sub1_DUF3591.
IPR001878. Znf_CCHC.
[Graphical view ]
Pfami PF12157. DUF3591. 1 hit.
[Graphical view ]
SMARTi SM00343. ZnF_C2HC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast TAFIIS in a multisubunit complex required for activated transcription."
    Reese J.C., Apone L., Walker S.S., Griffin L.A., Green M.R.
    Nature 371:523-527(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 583-599 AND 651-671.
    Strain: Y57.
  2. "The sequence of a 8 kb segment on the right arm of yeast chromosome VII identifies four new open reading frames and the genes for yTAFII145."
    Ruzzi M., Marconi A., Saliola M., Fabiani L., Montebove F., Frontali L.
    Yeast 13:365-368(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Identification and characterization of a TFIID-like multiprotein complex from Saccharomyces cerevisiae."
    Poon D., Bai Y., Campbell A.M., Bjorklund S., Kim Y.-J., Zhou S., Kornberg R.D., Weil P.A.
    Proc. Natl. Acad. Sci. U.S.A. 92:8224-8228(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 368-384; 528-554 AND 752-783, CHARACTERIZATION.
    Strain: ATCC 76621 / YPH252.
  6. Cited for: FUNCTION, TAF1 ACETYLATION ACTIVITY.
  7. "Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex."
    Sanders S.L., Weil P.A.
    J. Biol. Chem. 275:13895-13900(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  8. "Molecular characterization of Saccharomyces cerevisiae TFIID."
    Sanders S.L., Garbett K.A., Weil P.A.
    Mol. Cell. Biol. 22:6000-6013(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TFIID STOICHIOMETRY.
  9. "Multi-protein complexes in eukaryotic gene transcription."
    Martinez E.
    Plant Mol. Biol. 50:925-947(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  12. Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.

Entry informationi

Entry nameiTAF1_YEAST
AccessioniPrimary (citable) accession number: P46677
Secondary accession number(s): D6VV51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 26, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3