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P46677

- TAF1_YEAST

UniProt

P46677 - TAF1_YEAST

Protein

Transcription initiation factor TFIID subunit 1

Gene

TAF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.4 Publications

    Catalytic activityi

    Acetyl-CoA + [histone] = CoA + acetyl-[histone].

    GO - Molecular functioni

    1. chromatin binding Source: SGD
    2. histone acetyltransferase activity Source: SGD
    3. nucleic acid binding Source: InterPro
    4. protein binding Source: IntAct
    5. protein complex scaffold Source: SGD
    6. TBP-class protein binding Source: SGD
    7. zinc ion binding Source: InterPro

    GO - Biological processi

    1. histone acetylation Source: GOC
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
    4. transcription from RNA polymerase II promoter Source: SGD

    Keywords - Molecular functioni

    Acyltransferase, Chromatin regulator, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30939-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription initiation factor TFIID subunit 1 (EC:2.3.1.48)
    Alternative name(s):
    TAFII-130
    TAFII-145
    TBP-associated factor 1
    TBP-associated factor 145 kDa
    Gene namesi
    Name:TAF1
    Synonyms:TAF130, TAF145
    Ordered Locus Names:YGR274C
    ORF Names:G9374
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR274c.
    SGDiS000003506. TAF1.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. nucleus Source: SGD
    3. transcription factor TFIID complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10661066Transcription initiation factor TFIID subunit 1PRO_0000118862Add
    BLAST

    Proteomic databases

    MaxQBiP46677.
    PaxDbiP46677.
    PeptideAtlasiP46677.
    PRIDEiP46677.

    Expressioni

    Gene expression databases

    GenevestigatoriP46677.

    Interactioni

    Subunit structurei

    The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BDF1P358173EBI-18855,EBI-3493
    TAF2P232554EBI-18855,EBI-18862
    TAF4P501057EBI-18855,EBI-11231
    TAF6P530408EBI-18855,EBI-18876

    Protein-protein interaction databases

    BioGridi33524. 757 interactions.
    DIPiDIP-839N.
    IntActiP46677. 76 interactions.
    MINTiMINT-617790.
    STRINGi4932.YGR274C.

    Structurei

    Secondary structure

    1
    1066
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi15 – 239
    Beta strandi25 – 273
    Helixi28 – 358

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4B0AX-ray1.97A8-71[»]
    ProteinModelPortaliP46677.
    SMRiP46677. Positions 9-66.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili409 – 42517Sequence AnalysisAdd
    BLAST
    Coiled coili944 – 100057Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi65 – 695Poly-Asp

    Domaini

    Compared to higher eukaryotes TAF1, yeast TAF1 lacks the C-terminal bromodomains and C-terminal kinase activity. The TFIID interacting proteins BDF1 and BDF2 may substitute for these domains.

    Sequence similaritiesi

    Belongs to the TAF1 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5179.
    GeneTreeiENSGT00390000012659.
    HOGENOMiHOG000216192.
    KOiK03125.
    OMAiAPHSRKV.
    OrthoDBiEOG7PCJSB.

    Family and domain databases

    InterProiIPR022591. TFIID_sub1_DUF3591.
    IPR001878. Znf_CCHC.
    [Graphical view]
    PfamiPF12157. DUF3591. 1 hit.
    [Graphical view]
    SMARTiSM00343. ZnF_C2HC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46677-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVKQQGSGKT NLANEDEAYE AIFGGEFGSL EIGSYIGGDE GANSKDYTEH     50
    LPDAVDFEDE DELADDDDDL PEESDANLHP AMMTMGAYDD VNENGAVLGI 100
    DSNSLNMQLP EINGDLSQQF ILEDDGGTPA TSNALFMGMD ANEIHLATET 150
    GVLDGSGANE IGHSQLSIGG VNGNDMSING GFIMEPDMSD GKHKKATKLD 200
    LINHEKYLLK KYFPDFEKGK ILKWNKLIYR RSVPYHWHSE ISRVKKPFMP 250
    LNLKFKVQQD DKRLFNSRTI SYVAPIYQGK NNLLQSNSSA SRRGLIHVSI 300
    DELFPIKEQQ KKRKIIHDEK TISEDLLIAT DDWDQEKIIN QGTSSTATLA 350
    DSSMTPNLKF SGGYKLKSLI EDVAEDWQWD EDMIIDAKLK ESKHAELNMN 400
    DEKLLLMIEK TNNLAQQKQQ LDSSNLILPL NETILQQKFN LSNDDKYQIL 450
    KKTHQTKVRS TISNLNIQHS QPAINLQSPF YKVAVPRYQL RHFHRENFGS 500
    HIRPGTKIVF SKLKARKRKR DKGKDVKESF STSQDLTIGD TAPVYLMEYS 550
    EQTPVALSKF GMANKLINYY RKANEQDTLR PKLPVGETHV LGVQDKSPFW 600
    NFGFVEPGHI VPTLYNNMIR APVFKHDISG TDFLLTKSSG FGISNRFYLR 650
    NINHLFTVGQ TFPVEEIPGP NSRKVTSMKA TRLKMIIYRI LNHNHSKAIS 700
    IDPIAKHFPD QDYGQNRQKV KEFMKYQRDG PEKGLWRLKD DEKLLDNEAV 750
    KSLITPEQIS QVESMSQGLQ FQEDNEAYNF DSKLKSLEEN LLPWNITKNF 800
    INSTQMRAMI QIHGVGDPTG CGEGFSFLKT SMKGGFVKSG SPSSNNNSSN 850
    KKGTNTHSYN VAQQQKAYDE EIAKTWYTHT KSLSISNPFE EMTNPDEINQ 900
    TNKHVKTDRD DKKILKIVRK KRDENGIIQR QTIFIRDPRV IQGYIKIKEQ 950
    DKEDVNKLLE EDTSKINNLE ELEKQKKLLQ LELANLEKSQ QRRAARQNSK 1000
    RNGGATRTEN SVDNGSDLAG VTDGKAARNK GKNTTRRCAT CGQIGHIRTN 1050
    KSCPMYSSKD NPASPK 1066
    Length:1,066
    Mass (Da):120,696
    Last modified:November 1, 1995 - v1
    Checksum:iFAF1EE2A9B2A7B73
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14954 Genomic DNA. Translation: AAA79178.1.
    X84098 Genomic DNA. Translation: CAA58896.1.
    Z73059 Genomic DNA. Translation: CAA97304.1.
    BK006941 Genomic DNA. Translation: DAA08362.1.
    PIRiS50237.
    RefSeqiNP_011790.1. NM_001181403.2.

    Genome annotation databases

    EnsemblFungiiYGR274C; YGR274C; YGR274C.
    GeneIDi853191.
    KEGGisce:YGR274C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14954 Genomic DNA. Translation: AAA79178.1 .
    X84098 Genomic DNA. Translation: CAA58896.1 .
    Z73059 Genomic DNA. Translation: CAA97304.1 .
    BK006941 Genomic DNA. Translation: DAA08362.1 .
    PIRi S50237.
    RefSeqi NP_011790.1. NM_001181403.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4B0A X-ray 1.97 A 8-71 [» ]
    ProteinModelPortali P46677.
    SMRi P46677. Positions 9-66.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33524. 757 interactions.
    DIPi DIP-839N.
    IntActi P46677. 76 interactions.
    MINTi MINT-617790.
    STRINGi 4932.YGR274C.

    Proteomic databases

    MaxQBi P46677.
    PaxDbi P46677.
    PeptideAtlasi P46677.
    PRIDEi P46677.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR274C ; YGR274C ; YGR274C .
    GeneIDi 853191.
    KEGGi sce:YGR274C.

    Organism-specific databases

    CYGDi YGR274c.
    SGDi S000003506. TAF1.

    Phylogenomic databases

    eggNOGi COG5179.
    GeneTreei ENSGT00390000012659.
    HOGENOMi HOG000216192.
    KOi K03125.
    OMAi APHSRKV.
    OrthoDBi EOG7PCJSB.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30939-MONOMER.

    Miscellaneous databases

    NextBioi 973344.
    PROi P46677.

    Gene expression databases

    Genevestigatori P46677.

    Family and domain databases

    InterProi IPR022591. TFIID_sub1_DUF3591.
    IPR001878. Znf_CCHC.
    [Graphical view ]
    Pfami PF12157. DUF3591. 1 hit.
    [Graphical view ]
    SMARTi SM00343. ZnF_C2HC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Yeast TAFIIS in a multisubunit complex required for activated transcription."
      Reese J.C., Apone L., Walker S.S., Griffin L.A., Green M.R.
      Nature 371:523-527(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 583-599 AND 651-671.
      Strain: Y57.
    2. "The sequence of a 8 kb segment on the right arm of yeast chromosome VII identifies four new open reading frames and the genes for yTAFII145."
      Ruzzi M., Marconi A., Saliola M., Fabiani L., Montebove F., Frontali L.
      Yeast 13:365-368(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Identification and characterization of a TFIID-like multiprotein complex from Saccharomyces cerevisiae."
      Poon D., Bai Y., Campbell A.M., Bjorklund S., Kim Y.-J., Zhou S., Kornberg R.D., Weil P.A.
      Proc. Natl. Acad. Sci. U.S.A. 92:8224-8228(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 368-384; 528-554 AND 752-783, CHARACTERIZATION.
      Strain: ATCC 76621 / YPH252.
    6. Cited for: FUNCTION, TAF1 ACETYLATION ACTIVITY.
    7. "Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex."
      Sanders S.L., Weil P.A.
      J. Biol. Chem. 275:13895-13900(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    8. "Molecular characterization of Saccharomyces cerevisiae TFIID."
      Sanders S.L., Garbett K.A., Weil P.A.
      Mol. Cell. Biol. 22:6000-6013(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TFIID STOICHIOMETRY.
    9. "Multi-protein complexes in eukaryotic gene transcription."
      Martinez E.
      Plant Mol. Biol. 50:925-947(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    12. Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.

    Entry informationi

    Entry nameiTAF1_YEAST
    AccessioniPrimary (citable) accession number: P46677
    Secondary accession number(s): D6VV51
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1500 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3