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Protein

Transcription initiation factor TFIID subunit 1

Gene

TAF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.4 Publications

Catalytic activityi

Acetyl-CoA + [histone] = CoA + acetyl-[histone].

GO - Molecular functioni

  • chromatin binding Source: SGD
  • histone acetyltransferase activity Source: SGD
  • protein complex scaffold Source: SGD
  • protein heterodimerization activity Source: ParkinsonsUK-UCL
  • RNA polymerase II transcription factor activity, TBP-class protein binding, involved in preinitiation complex assembly Source: GO_Central
  • sequence-specific DNA binding Source: GO_Central
  • TBP-class protein binding Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Chromatin regulator, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30939-MONOMER.
ReactomeiR-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 1 (EC:2.3.1.48)
Alternative name(s):
TAFII-130
TAFII-145
TBP-associated factor 1
TBP-associated factor 145 kDa
Gene namesi
Name:TAF1
Synonyms:TAF130, TAF145
Ordered Locus Names:YGR274C
ORF Names:G9374
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR274C.
SGDiS000003506. TAF1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: SGD
  • transcription factor TFIID complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001188621 – 1066Transcription initiation factor TFIID subunit 1Add BLAST1066

Proteomic databases

MaxQBiP46677.
PRIDEiP46677.

PTM databases

iPTMnetiP46677.

Interactioni

Subunit structurei

The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BDF1P358173EBI-18855,EBI-3493
TAF2P232554EBI-18855,EBI-18862
TAF4P501057EBI-18855,EBI-11231
TAF6P530408EBI-18855,EBI-18876

GO - Molecular functioni

  • protein complex scaffold Source: SGD
  • protein heterodimerization activity Source: ParkinsonsUK-UCL
  • TBP-class protein binding Source: SGD

Protein-protein interaction databases

BioGridi33524. 756 interactors.
DIPiDIP-839N.
IntActiP46677. 76 interactors.
MINTiMINT-617790.

Structurei

Secondary structure

11066
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 23Combined sources9
Beta strandi25 – 27Combined sources3
Helixi28 – 35Combined sources8
Helixi471 – 475Combined sources5
Turni478 – 480Combined sources3
Beta strandi481 – 484Combined sources4
Helixi488 – 491Combined sources4
Turni492 – 494Combined sources3
Turni498 – 501Combined sources4
Beta strandi507 – 510Combined sources4
Helixi518 – 521Combined sources4
Helixi526 – 529Combined sources4
Helixi533 – 536Combined sources4
Beta strandi538 – 542Combined sources5
Beta strandi544 – 553Combined sources10
Beta strandi563 – 571Combined sources9
Beta strandi584 – 591Combined sources8
Turni598 – 601Combined sources4
Beta strandi610 – 615Combined sources6
Beta strandi617 – 626Combined sources10
Beta strandi632 – 640Combined sources9
Beta strandi643 – 650Combined sources8
Beta strandi653 – 658Combined sources6
Beta strandi662 – 665Combined sources4
Helixi673 – 694Combined sources22
Helixi702 – 705Combined sources4
Helixi713 – 720Combined sources8
Turni721 – 723Combined sources3
Beta strandi724 – 726Combined sources3
Turni731 – 734Combined sources4
Beta strandi735 – 738Combined sources4
Helixi747 – 750Combined sources4
Helixi751 – 753Combined sources3
Helixi756 – 778Combined sources23
Helixi782 – 790Combined sources9
Helixi793 – 804Combined sources12
Beta strandi820 – 824Combined sources5
Helixi859 – 882Combined sources24
Helixi888 – 891Combined sources4
Helixi900 – 903Combined sources4
Beta strandi914 – 922Combined sources9
Beta strandi926 – 935Combined sources10
Helixi938 – 951Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4B0AX-ray1.97A8-71[»]
4OY2X-ray2.90A/C/E455-960[»]
ProteinModelPortaliP46677.
SMRiP46677.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili409 – 425Sequence analysisAdd BLAST17
Coiled coili944 – 1000Sequence analysisAdd BLAST57

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi65 – 69Poly-Asp5

Domaini

Compared to higher eukaryotes TAF1, yeast TAF1 lacks the C-terminal bromodomains and C-terminal kinase activity. The TFIID interacting proteins BDF1 and BDF2 may substitute for these domains.

Sequence similaritiesi

Belongs to the TAF1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000012659.
HOGENOMiHOG000216192.
InParanoidiP46677.
KOiK03125.
OMAiKAYDEEI.
OrthoDBiEOG092C0BPW.

Family and domain databases

InterProiIPR022591. TFIID_sub1_DUF3591.
[Graphical view]
PfamiPF12157. DUF3591. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46677-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKQQGSGKT NLANEDEAYE AIFGGEFGSL EIGSYIGGDE GANSKDYTEH
60 70 80 90 100
LPDAVDFEDE DELADDDDDL PEESDANLHP AMMTMGAYDD VNENGAVLGI
110 120 130 140 150
DSNSLNMQLP EINGDLSQQF ILEDDGGTPA TSNALFMGMD ANEIHLATET
160 170 180 190 200
GVLDGSGANE IGHSQLSIGG VNGNDMSING GFIMEPDMSD GKHKKATKLD
210 220 230 240 250
LINHEKYLLK KYFPDFEKGK ILKWNKLIYR RSVPYHWHSE ISRVKKPFMP
260 270 280 290 300
LNLKFKVQQD DKRLFNSRTI SYVAPIYQGK NNLLQSNSSA SRRGLIHVSI
310 320 330 340 350
DELFPIKEQQ KKRKIIHDEK TISEDLLIAT DDWDQEKIIN QGTSSTATLA
360 370 380 390 400
DSSMTPNLKF SGGYKLKSLI EDVAEDWQWD EDMIIDAKLK ESKHAELNMN
410 420 430 440 450
DEKLLLMIEK TNNLAQQKQQ LDSSNLILPL NETILQQKFN LSNDDKYQIL
460 470 480 490 500
KKTHQTKVRS TISNLNIQHS QPAINLQSPF YKVAVPRYQL RHFHRENFGS
510 520 530 540 550
HIRPGTKIVF SKLKARKRKR DKGKDVKESF STSQDLTIGD TAPVYLMEYS
560 570 580 590 600
EQTPVALSKF GMANKLINYY RKANEQDTLR PKLPVGETHV LGVQDKSPFW
610 620 630 640 650
NFGFVEPGHI VPTLYNNMIR APVFKHDISG TDFLLTKSSG FGISNRFYLR
660 670 680 690 700
NINHLFTVGQ TFPVEEIPGP NSRKVTSMKA TRLKMIIYRI LNHNHSKAIS
710 720 730 740 750
IDPIAKHFPD QDYGQNRQKV KEFMKYQRDG PEKGLWRLKD DEKLLDNEAV
760 770 780 790 800
KSLITPEQIS QVESMSQGLQ FQEDNEAYNF DSKLKSLEEN LLPWNITKNF
810 820 830 840 850
INSTQMRAMI QIHGVGDPTG CGEGFSFLKT SMKGGFVKSG SPSSNNNSSN
860 870 880 890 900
KKGTNTHSYN VAQQQKAYDE EIAKTWYTHT KSLSISNPFE EMTNPDEINQ
910 920 930 940 950
TNKHVKTDRD DKKILKIVRK KRDENGIIQR QTIFIRDPRV IQGYIKIKEQ
960 970 980 990 1000
DKEDVNKLLE EDTSKINNLE ELEKQKKLLQ LELANLEKSQ QRRAARQNSK
1010 1020 1030 1040 1050
RNGGATRTEN SVDNGSDLAG VTDGKAARNK GKNTTRRCAT CGQIGHIRTN
1060
KSCPMYSSKD NPASPK
Length:1,066
Mass (Da):120,696
Last modified:November 1, 1995 - v1
Checksum:iFAF1EE2A9B2A7B73
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14954 Genomic DNA. Translation: AAA79178.1.
X84098 Genomic DNA. Translation: CAA58896.1.
Z73059 Genomic DNA. Translation: CAA97304.1.
BK006941 Genomic DNA. Translation: DAA08362.1.
PIRiS50237.
RefSeqiNP_011790.1. NM_001181403.2.

Genome annotation databases

EnsemblFungiiYGR274C; YGR274C; YGR274C.
GeneIDi853191.
KEGGisce:YGR274C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14954 Genomic DNA. Translation: AAA79178.1.
X84098 Genomic DNA. Translation: CAA58896.1.
Z73059 Genomic DNA. Translation: CAA97304.1.
BK006941 Genomic DNA. Translation: DAA08362.1.
PIRiS50237.
RefSeqiNP_011790.1. NM_001181403.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4B0AX-ray1.97A8-71[»]
4OY2X-ray2.90A/C/E455-960[»]
ProteinModelPortaliP46677.
SMRiP46677.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33524. 756 interactors.
DIPiDIP-839N.
IntActiP46677. 76 interactors.
MINTiMINT-617790.

PTM databases

iPTMnetiP46677.

Proteomic databases

MaxQBiP46677.
PRIDEiP46677.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR274C; YGR274C; YGR274C.
GeneIDi853191.
KEGGisce:YGR274C.

Organism-specific databases

EuPathDBiFungiDB:YGR274C.
SGDiS000003506. TAF1.

Phylogenomic databases

GeneTreeiENSGT00390000012659.
HOGENOMiHOG000216192.
InParanoidiP46677.
KOiK03125.
OMAiKAYDEEI.
OrthoDBiEOG092C0BPW.

Enzyme and pathway databases

BioCyciYEAST:G3O-30939-MONOMER.
ReactomeiR-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Miscellaneous databases

PROiP46677.

Family and domain databases

InterProiIPR022591. TFIID_sub1_DUF3591.
[Graphical view]
PfamiPF12157. DUF3591. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTAF1_YEAST
AccessioniPrimary (citable) accession number: P46677
Secondary accession number(s): D6VV51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.