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P46675

- STU2_YEAST

UniProt

P46675 - STU2_YEAST

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Protein
Protein STU2
Gene
STU2, YLR045C, L2108
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May play a role in the attachment, organization, and/or dynamics of microtubule ends at the spindle pole body.

GO - Molecular functioni

  1. microtubule binding Source: SGD
  2. structural constituent of cytoskeleton Source: SGD

GO - Biological processi

  1. microtubule nucleation Source: SGD
  2. mitotic spindle organization in nucleus Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-32201-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein STU2
Alternative name(s):
Suppressor of tubulin 2
Gene namesi
Name:STU2
Ordered Locus Names:YLR045C
ORF Names:L2108
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR045c.
SGDiS000004035. STU2.

Subcellular locationi

Cytoplasmcytoskeletonmicrotubule organizing centerspindle pole body. Cytoplasmcytoskeletonspindle
Note: Localizes primarily to the spindle pole body (SPB) and to a lesser extent along spindle microtubules.

GO - Cellular componenti

  1. cell cortex Source: SGD
  2. condensed nuclear chromosome kinetochore Source: SGD
  3. kinetochore Source: SGD
  4. spindle microtubule Source: SGD
  5. spindle pole body Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 888888Protein STU2
PRO_0000072295Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21Phosphoserine1 Publication
Modified residuei277 – 2771Phosphoserine1 Publication
Modified residuei813 – 8131Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP46675.
PaxDbiP46675.
PeptideAtlasiP46675.

Expressioni

Gene expression databases

GenevestigatoriP46675.

Interactioni

Subunit structurei

Binds to microtubules. Interacts with SPC72.1 Publication

Protein-protein interaction databases

BioGridi31320. 113 interactions.
DIPiDIP-2462N.
IntActiP46675. 44 interactions.
MINTiMINT-501107.
STRINGi4932.YLR045C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi14 – 174
Helixi23 – 3816
Helixi58 – 636
Helixi69 – 8315
Helixi93 – 11018
Helixi117 – 13216
Beta strandi134 – 1374
Helixi138 – 1447
Helixi145 – 1495
Helixi153 – 17018
Turni171 – 1744
Helixi177 – 1848
Helixi185 – 1873
Helixi188 – 1925
Helixi197 – 21115
Helixi231 – 24111
Helixi256 – 26813
Helixi325 – 3273
Helixi332 – 3365
Helixi341 – 35414
Helixi356 – 3583
Helixi370 – 38213
Helixi386 – 40318
Turni405 – 4073
Helixi410 – 42213
Helixi423 – 4253
Helixi429 – 44517
Helixi456 – 46510
Helixi471 – 48717
Helixi493 – 4997
Turni500 – 5023
Helixi503 – 5119
Helixi516 – 53318
Helixi536 – 5383
Helixi539 – 5446
Helixi547 – 55812

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QK1X-ray1.70A317-560[»]
4FFBX-ray2.88C1-272[»]
ProteinModelPortaliP46675.
SMRiP46675. Positions 11-272, 317-559.

Miscellaneous databases

EvolutionaryTraceiP46675.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati453 – 49038HEAT 1
Add
BLAST
Repeati498 – 53538HEAT 2
Add
BLAST

Sequence similaritiesi

Belongs to the TOG/XMAP215 family.
Contains 2 HEAT repeats.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG253097.
GeneTreeiENSGT00390000014757.
HOGENOMiHOG000216643.
KOiK16803.
OMAiPQIRMEC.
OrthoDBiEOG7PK97G.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46675-1 [UniParc]FASTAAdd to Basket

« Hide

MSGEEEVDYT TLPLEERLTY KLWKARLEAY KELNQLFRNS VGDISRDDNI    50
QIYWRDPTLF AQYITDSNVV AQEQAIVALN SLIDAFASSS LKNAHNITLI 100
STWTPLLVEK GLTSSRATTK TQSMSCILSL CGLDTSITQS VELVIPFFEK 150
KLPKLIAAAA NCVYELMAAF GLTNVNVQTF LPELLKHVPQ LAGHGDRNVR 200
SQTMNLIVEI YKVTGNNSDL LEEILFKKLK PIQVKDLHKL FAKVGDEPSS 250
SKMLFEWEKR ELEKKRSQEE EARKRKSILS NDEGEYQIDK DGDTLMGMET 300
DMPPSKQQSG VQIDTFSMLP EETILDKLPK DFQERITSSK WKDRVEALEE 350
FWDSVLSQTK KLKSTSQNYS NLLGIYGHII QKDANIQAVA LAAQSVELIC 400
DKLKTPGFSK DYVSLVFTPL LDRTKEKKPS VIEAIRKALL TICKYYDPLA 450
SSGRNEDMLK DILEHMKHKT PQIRMECTQL FNASMKEEKD GYSTLQRYLK 500
DEVVPIVIQI VNDTQPAIRT IGFESFAILI KIFGMNTFVK TLEHLDNLKR 550
KKIEETVKTL PNFSIASGST HSTIETNKQT GPMENKFLLK KSSVLPSKRV 600
ASSPLRNDNK SKVNPIGSVA SASKPSMVAA NNKSRVLLTS KSLATPKNVV 650
ANSTDKNEKL IEEYKYRLQK LQNDEMIWTK ERQSLLEKMN NTENYKIEMI 700
KENEMLREQL KEAQSKLNEK NIQLRSKEID VNKLSDRVLS LENELRNMEI 750
ELDRNKKRND TNLQSMGTIS SYSIPSSTVS SNYGVKSLSS ALPFKEEEDV 800
RRKEDVNYER RSSESIGDLP HRVNSLNIRP YRKNGTGVSS VSDDLDIDFN 850
DSFASEESYK RAAAVTSTLK ARIEKMKAKS RREGTTRT 888
Length:888
Mass (Da):100,918
Last modified:November 1, 1995 - v1
Checksum:i7A49C3702E21C7BF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U35247 Genomic DNA. Translation: AAA79057.1.
X94607 Genomic DNA. Translation: CAA64292.1.
Z73217 Genomic DNA. Translation: CAA97574.1.
BK006945 Genomic DNA. Translation: DAA09363.1.
PIRiS61619.
RefSeqiNP_013146.1. NM_001181932.1.

Genome annotation databases

EnsemblFungiiYLR045C; YLR045C; YLR045C.
GeneIDi850734.
KEGGisce:YLR045C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U35247 Genomic DNA. Translation: AAA79057.1 .
X94607 Genomic DNA. Translation: CAA64292.1 .
Z73217 Genomic DNA. Translation: CAA97574.1 .
BK006945 Genomic DNA. Translation: DAA09363.1 .
PIRi S61619.
RefSeqi NP_013146.1. NM_001181932.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2QK1 X-ray 1.70 A 317-560 [» ]
4FFB X-ray 2.88 C 1-272 [» ]
ProteinModelPortali P46675.
SMRi P46675. Positions 11-272, 317-559.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31320. 113 interactions.
DIPi DIP-2462N.
IntActi P46675. 44 interactions.
MINTi MINT-501107.
STRINGi 4932.YLR045C.

Proteomic databases

MaxQBi P46675.
PaxDbi P46675.
PeptideAtlasi P46675.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR045C ; YLR045C ; YLR045C .
GeneIDi 850734.
KEGGi sce:YLR045C.

Organism-specific databases

CYGDi YLR045c.
SGDi S000004035. STU2.

Phylogenomic databases

eggNOGi NOG253097.
GeneTreei ENSGT00390000014757.
HOGENOMi HOG000216643.
KOi K16803.
OMAi PQIRMEC.
OrthoDBi EOG7PK97G.

Enzyme and pathway databases

BioCyci YEAST:G3O-32201-MONOMER.

Miscellaneous databases

EvolutionaryTracei P46675.
NextBioi 966834.
PROi P46675.

Gene expression databases

Genevestigatori P46675.

Family and domain databases

Gene3Di 1.25.10.10. 2 hits.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS50077. HEAT_REPEAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Stu2p: a microtubule-binding protein that is an essential component of the yeast spindle pole body."
    Wang P.J., Huffaker T.C.
    J. Cell Biol. 139:1271-1280(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CUY696.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The yeast spindle pole body component Spc72p interacts with Stu2p and is required for proper microtubule assembly."
    Chen X.P., Yin H., Huffaker T.C.
    J. Cell Biol. 141:1169-1179(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SPC72.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-813, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-277 AND SER-813, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSTU2_YEAST
AccessioniPrimary (citable) accession number: P46675
Secondary accession number(s): D6VY47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 14, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Mutations in STU2 suppress a cold-sensitive mutation in TUB2.
Present with 1660 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

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