Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P46675 (STU2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein STU2
Alternative name(s):
Suppressor of tubulin 2
Gene names
Name:STU2
Ordered Locus Names:YLR045C
ORF Names:L2108
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length888 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in the attachment, organization, and/or dynamics of microtubule ends at the spindle pole body.

Subunit structure

Binds to microtubules. Interacts with SPC72. Ref.4

Subcellular location

Cytoplasmcytoskeletonmicrotubule organizing centerspindle pole body. Cytoplasmcytoskeletonspindle. Note: Localizes primarily to the spindle pole body (SPB) and to a lesser extent along spindle microtubules.

Miscellaneous

Mutations in STU2 suppress a cold-sensitive mutation in TUB2.

Present with 1660 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TOG/XMAP215 family.

Contains 2 HEAT repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 888888Protein STU2
PRO_0000072295

Regions

Repeat453 – 49038HEAT 1
Repeat498 – 53538HEAT 2

Amino acid modifications

Modified residue21Phosphoserine Ref.8
Modified residue2771Phosphoserine Ref.9
Modified residue8131Phosphoserine Ref.6 Ref.9

Secondary structure

................................................................ 888
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P46675 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 7A49C3702E21C7BF

FASTA888100,918
        10         20         30         40         50         60 
MSGEEEVDYT TLPLEERLTY KLWKARLEAY KELNQLFRNS VGDISRDDNI QIYWRDPTLF 

        70         80         90        100        110        120 
AQYITDSNVV AQEQAIVALN SLIDAFASSS LKNAHNITLI STWTPLLVEK GLTSSRATTK 

       130        140        150        160        170        180 
TQSMSCILSL CGLDTSITQS VELVIPFFEK KLPKLIAAAA NCVYELMAAF GLTNVNVQTF 

       190        200        210        220        230        240 
LPELLKHVPQ LAGHGDRNVR SQTMNLIVEI YKVTGNNSDL LEEILFKKLK PIQVKDLHKL 

       250        260        270        280        290        300 
FAKVGDEPSS SKMLFEWEKR ELEKKRSQEE EARKRKSILS NDEGEYQIDK DGDTLMGMET 

       310        320        330        340        350        360 
DMPPSKQQSG VQIDTFSMLP EETILDKLPK DFQERITSSK WKDRVEALEE FWDSVLSQTK 

       370        380        390        400        410        420 
KLKSTSQNYS NLLGIYGHII QKDANIQAVA LAAQSVELIC DKLKTPGFSK DYVSLVFTPL 

       430        440        450        460        470        480 
LDRTKEKKPS VIEAIRKALL TICKYYDPLA SSGRNEDMLK DILEHMKHKT PQIRMECTQL 

       490        500        510        520        530        540 
FNASMKEEKD GYSTLQRYLK DEVVPIVIQI VNDTQPAIRT IGFESFAILI KIFGMNTFVK 

       550        560        570        580        590        600 
TLEHLDNLKR KKIEETVKTL PNFSIASGST HSTIETNKQT GPMENKFLLK KSSVLPSKRV 

       610        620        630        640        650        660 
ASSPLRNDNK SKVNPIGSVA SASKPSMVAA NNKSRVLLTS KSLATPKNVV ANSTDKNEKL 

       670        680        690        700        710        720 
IEEYKYRLQK LQNDEMIWTK ERQSLLEKMN NTENYKIEMI KENEMLREQL KEAQSKLNEK 

       730        740        750        760        770        780 
NIQLRSKEID VNKLSDRVLS LENELRNMEI ELDRNKKRND TNLQSMGTIS SYSIPSSTVS 

       790        800        810        820        830        840 
SNYGVKSLSS ALPFKEEEDV RRKEDVNYER RSSESIGDLP HRVNSLNIRP YRKNGTGVSS 

       850        860        870        880 
VSDDLDIDFN DSFASEESYK RAAAVTSTLK ARIEKMKAKS RREGTTRT 

« Hide

References

« Hide 'large scale' references
[1]"Stu2p: a microtubule-binding protein that is an essential component of the yeast spindle pole body."
Wang P.J., Huffaker T.C.
J. Cell Biol. 139:1271-1280(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: CUY696.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The yeast spindle pole body component Spc72p interacts with Stu2p and is required for proper microtubule assembly."
Chen X.P., Yin H., Huffaker T.C.
J. Cell Biol. 141:1169-1179(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SPC72.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-813, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-277 AND SER-813, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U35247 Genomic DNA. Translation: AAA79057.1.
X94607 Genomic DNA. Translation: CAA64292.1.
Z73217 Genomic DNA. Translation: CAA97574.1.
BK006945 Genomic DNA. Translation: DAA09363.1.
PIRS61619.
RefSeqNP_013146.1. NM_001181932.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2QK1X-ray1.70A317-560[»]
4FFBX-ray2.88C1-272[»]
ProteinModelPortalP46675.
SMRP46675. Positions 11-272, 317-559.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31320. 113 interactions.
DIPDIP-2462N.
IntActP46675. 44 interactions.
MINTMINT-501107.
STRING4932.YLR045C.

Proteomic databases

MaxQBP46675.
PaxDbP46675.
PeptideAtlasP46675.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR045C; YLR045C; YLR045C.
GeneID850734.
KEGGsce:YLR045C.

Organism-specific databases

CYGDYLR045c.
SGDS000004035. STU2.

Phylogenomic databases

eggNOGNOG253097.
GeneTreeENSGT00390000014757.
HOGENOMHOG000216643.
KOK16803.
OMAPQIRMEC.
OrthoDBEOG7PK97G.

Enzyme and pathway databases

BioCycYEAST:G3O-32201-MONOMER.

Gene expression databases

GenevestigatorP46675.

Family and domain databases

Gene3D1.25.10.10. 2 hits.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
PROSITEPS50077. HEAT_REPEAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP46675.
NextBio966834.
PROP46675.

Entry information

Entry nameSTU2_YEAST
AccessionPrimary (citable) accession number: P46675
Secondary accession number(s): D6VY47
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 14, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references