Protein STU2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P46675 (STU2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 113. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein STU2

Alternative name(s):
Suppressor of tubulin 2

Gene names

Name:	STU2
Ordered Locus Names:	YLR045C
ORF Names:	L2108

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	888 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in the attachment, organization, and/or dynamics of microtubule ends at the spindle pole body.
Subunit structure	Binds to microtubules. Interacts with SPC72. Ref.4
Subcellular location	Cytoplasm › cytoskeleton › spindle pole body. Cytoplasm › cytoskeleton › spindle. Note: Localizes primarily to the spindle pole body (SPB) and to a lesser extent along spindle microtubules.
Miscellaneous	Mutations in STU2 suppress a cold-sensitive mutation in TUB2. Present with 1660 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the TOG/XMAP215 family. Contains 2 HEAT repeats.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Domain	Repeat
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	microtubule nucleation Inferred from physical interaction PubMed 9153752. Source: SGD mitotic spindle organization in nucleus Inferred from genetic interaction PubMed 11756468. Source: SGD
Cellular_component	cell cortex Inferred from direct assay PubMed 11511347. Source: SGD condensed nuclear chromosome kinetochore Inferred from direct assay PubMed 11511347. Source: SGD spindle microtubule Inferred from direct assay PubMed 11511347. Source: SGD spindle pole body Inferred from direct assay Ref.4. Source: SGD
Molecular_function	microtubule binding Inferred from mutant phenotype PubMed 11553724. Source: SGD structural constituent of cytoskeleton Inferred from physical interaction Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 888

888

Protein STU2

PRO_0000072295

Regions

Repeat

453 – 490

HEAT 1

Repeat

498 – 535

HEAT 2

Amino acid modifications

Modified residue

Phosphoserine Ref.9

Modified residue

251

Phosphoserine Ref.7

Modified residue

603

Phosphoserine Ref.7 Ref.8 Ref.9

Modified residue

812

Phosphoserine Ref.8 Ref.9

Modified residue

813

Phosphoserine Ref.6 Ref.8 Ref.9

Modified residue

815

Phosphoserine Ref.9

Modified residue

825

Phosphoserine Ref.9

Secondary structure

888

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P46675 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 7A49C3702E21C7BF
Show »

FASTA

888

100,918

        10         20         30         40         50         60 
MSGEEEVDYT TLPLEERLTY KLWKARLEAY KELNQLFRNS VGDISRDDNI QIYWRDPTLF 

        70         80         90        100        110        120 
AQYITDSNVV AQEQAIVALN SLIDAFASSS LKNAHNITLI STWTPLLVEK GLTSSRATTK 

       130        140        150        160        170        180 
TQSMSCILSL CGLDTSITQS VELVIPFFEK KLPKLIAAAA NCVYELMAAF GLTNVNVQTF 

       190        200        210        220        230        240 
LPELLKHVPQ LAGHGDRNVR SQTMNLIVEI YKVTGNNSDL LEEILFKKLK PIQVKDLHKL 

       250        260        270        280        290        300 
FAKVGDEPSS SKMLFEWEKR ELEKKRSQEE EARKRKSILS NDEGEYQIDK DGDTLMGMET 

       310        320        330        340        350        360 
DMPPSKQQSG VQIDTFSMLP EETILDKLPK DFQERITSSK WKDRVEALEE FWDSVLSQTK 

       370        380        390        400        410        420 
KLKSTSQNYS NLLGIYGHII QKDANIQAVA LAAQSVELIC DKLKTPGFSK DYVSLVFTPL 

       430        440        450        460        470        480 
LDRTKEKKPS VIEAIRKALL TICKYYDPLA SSGRNEDMLK DILEHMKHKT PQIRMECTQL 

       490        500        510        520        530        540 
FNASMKEEKD GYSTLQRYLK DEVVPIVIQI VNDTQPAIRT IGFESFAILI KIFGMNTFVK 

       550        560        570        580        590        600 
TLEHLDNLKR KKIEETVKTL PNFSIASGST HSTIETNKQT GPMENKFLLK KSSVLPSKRV 

       610        620        630        640        650        660 
ASSPLRNDNK SKVNPIGSVA SASKPSMVAA NNKSRVLLTS KSLATPKNVV ANSTDKNEKL 

       670        680        690        700        710        720 
IEEYKYRLQK LQNDEMIWTK ERQSLLEKMN NTENYKIEMI KENEMLREQL KEAQSKLNEK 

       730        740        750        760        770        780 
NIQLRSKEID VNKLSDRVLS LENELRNMEI ELDRNKKRND TNLQSMGTIS SYSIPSSTVS 

       790        800        810        820        830        840 
SNYGVKSLSS ALPFKEEEDV RRKEDVNYER RSSESIGDLP HRVNSLNIRP YRKNGTGVSS 

       850        860        870        880 
VSDDLDIDFN DSFASEESYK RAAAVTSTLK ARIEKMKAKS RREGTTRT

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Stu2p: a microtubule-binding protein that is an essential component of the yeast spindle pole body." Wang P.J., Huffaker T.C. J. Cell Biol. 139:1271-1280(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CUY696.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The yeast spindle pole body component Spc72p interacts with Stu2p and is required for proper microtubule assembly." Chen X.P., Yin H., Huffaker T.C. J. Cell Biol. 141:1169-1179(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SPC72.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-813, MASS SPECTROMETRY. Strain: ADR376.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-251 AND SER-603, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-603; SER-812 AND SER-813, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2; SER-603; SER-812; SER-813; SER-815 AND SER-825, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U35247 Genomic DNA. Translation: AAA79057.1.
X94607 Genomic DNA. Translation: CAA64292.1.
Z73217 Genomic DNA. Translation: CAA97574.1.
BK006945 Genomic DNA. Translation: DAA09363.1.

PIR

S61619.

RefSeq

NP_013146.1. NM_001181932.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2QK1	X-ray	1.70	A	317-560	[»]
4FFB	X-ray	2.88	C	1-272	[»]

ProteinModelPortal

P46675.

SMR

P46675. Positions 11-272, 317-559.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2462N.

IntAct

P46675. 44 interactions.

MINT

MINT-501107.

STRING

4932.YLR045C.

Proteomic databases

PaxDb

P46675.

PeptideAtlas

P46675.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YLR045C; YLR045C; YLR045C.

GeneID

850734.

KEGG

sce:YLR045C.

Organism-specific databases

CYGD

YLR045c.

SGD

S000004035. STU2.

Phylogenomic databases

eggNOG

NOG253097.

GeneTree

ENSGT00390000014757.

HOGENOM

HOG000216643.

K16803.

OMA

SKWKDRV.

OrthoDB

EOG4NGKWH.

Enzyme and pathway databases

BioCyc

YEAST:G3O-32201-MONOMER.

Gene expression databases

Genevestigator

P46675.

GermOnline

YLR045C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.25.10.10. 2 hits.

InterPro

IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
[Graphical view]

SUPFAM

SSF48371. ARM-type_fold. 1 hit.

PROSITE

PS50077. HEAT_REPEAT. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P46675.

NextBio

966834.

Entry information

Entry name

STU2_YEAST

Accession

Primary (citable) accession number: P46675
Secondary accession number(s): D6VY47

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 29, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents